GhRCD1 promotes cotton tolerance to cadmium by regulating the GhbHLH12–GhMYB44–GhHMA1 transcriptional cascade

Plant Biotechnology Journal, Год журнала: 2024, Номер 22(7), С. 1777 - 1796

Опубликована: Фев. 13, 2024

Summary Heavy metal pollution poses a significant risk to human health and wreaks havoc on agricultural productivity. Phytoremediation, plant‐based, environmentally benign, cost‐effective method, is employed remove heavy metals from contaminated soil, particularly in or metal‐sensitive lands. However, the phytoremediation capacity of various plant species germplasm resources display genetic diversity, mechanisms underlying these differences remain hitherto obscure. Given its potential benefits, improvement plants essential for enhancing their uptake metals, tolerance harmful levels, as well overall growth development soil. In this study, we uncover molecular cascade that regulates cadmium (Cd 2+ ) cotton, involving GhRCD1, GhbHLH12, GhMYB44, GhHMA1. We identified Cd ‐sensitive cotton T‐DNA insertion mutant with disrupted GhRCD1 expression. Genetic knockout by CRISPR/Cas9 technology resulted reduced seedlings, while overexpression enhanced tolerance. Through interaction studies, demonstrated that, response presence, directly interacts GhbHLH12. This activates which subsequently transporter, GhHMA1 , binding G‐box cis ‐element promoter. These findings provide critical insights into novel GhRCD1–GhbHLH12–GhMYB44–GhHMA1 regulatory module responsible cotton. Furthermore, our study paves way elite ‐tolerant cultivars elucidating governing control

Язык: Английский

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QTL mapping and BSA-seq map a major QTL for the node of the first fruiting branch in cotton

Frontiers in Plant Science, Год журнала: 2023, Номер 14

Опубликована: Янв. 25, 2023

Understanding the genetic basis of node first fruiting branch (NFFB) improves early-maturity cotton breeding. Here we report QTL mapping on 200 F 2 plants and derivative 2:3 2:4 populations by genotyping sequencing (GBS). BC 1 population was constructed backcrossing one line with maternal parent JF914 used for BSA-seq further mapping. A total 1,305,642 SNPs were developed between parents GBS, 2,907,790 detected BSA-seq. high-density map containing 11,488 spanning 4,202.12 cM in length. 13 mapped 3 tested populations. conferred favorable alleles 11 QTL, JF173 other QTL. Two stable repeatedly 2:4, including qNFFB-D3-1 qNFFB-D6-1 . Only contributed more than 10% phenotypic variation. This covered about 24.7 Mb (17,130,008–41,839,226 bp) chromosome D3. regions D3 (41,779,195–41,836,120 bp, 41,836,768–41,872,287 found 92.4 Kb. Kb region overlapped contained 8 annotated genes. By qRT-PCR, Ghir_D03G012430 showed a lower expression level from 1- to 2-leaf stage higher 3- 6-leaf buds that JF914. Ghir_D03G012390 reached highest at 5-leaf stages JF914, respectively. As has NFFB early maturity these two genes might be important cell division differentiation during formation seedling stage. The results this study will facilitate better understanding benefit molecular breeding improving earliness traits.

Язык: Английский

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Morphological and physio-biochemical responses under heat stress in cotton: Overview

Biotechnology Reports, Год журнала: 2023, Номер 40, С. e00813 - e00813

Опубликована: Сен. 14, 2023

Cotton is an important cash crop in addition to being a fiber commodity, and it plays essential part the economies of numerous nations. High temperature most critical element affecting its yield from fertilization harvest. The optimal for root formation 30 C -35 °C; however, development ends around 40 °C. Increased temperature, particular, influences different biochemical physiological processes associated with cotton plant, resulting low seed production. Many studies various agroecological zones used agronomic strategies contemporary breeding techniques reduce heat stress improve productivity. To attain desired traits, breeders should investigate all potential possibilities, such as generating superior cultivars by traditional breeding, employing molecular transgenic methods, using genome editing techniques. main objective this review provide recent information on environmental factors, drought, influence growth development, morphology physio-chemical alteration cotton. Furthermore, advancement combat serious threat drought stress.

Язык: Английский

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Synergistic optimization of crops by combining early maturation with other agronomic traits

Trends in Plant Science, Год журнала: 2023, Номер 28(10), С. 1178 - 1191

Опубликована: Май 18, 2023

Язык: Английский

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Identification of SSR markers linked to the abscission of cotton boll traits and mining germplasm in Cotton

Journal of Cotton Research, Год журнала: 2024, Номер 7(1)

Опубликована: Июнь 5, 2024

Abstract Background Cotton is an economically important crop. It crucial to find effective method improve cotton yield, and one approach decrease the abscission of bolls buds. However, lack knowledge genetic molecular mechanisms underlying boll traits has hindered improvements. Results Pearson’s correlation analysis revealed a significant positive between rates 1 (AR1) 2 (AR2). A genome-wide association study was conducted on 145 loci that exhibited high polymorphism were uniformly distributed across 26 chromosomes (pair). The 18, 46, 62 markers significantly associated with abscission, fiber quality, yield ( P < 0.05), explaining 1.75%–7.13%, 1.16%–9.58%, 1.40%–5.44% phenotypic variation, respectively. Notably, marker MON_SHIN-1584b trait, whereas MON_CGR5732a quality traits. Thirteen identified in this had been previously reported. Based effects, six typical cultivars elite alleles related identified. These hold great promise for widespread utilization breeding programs. Conclusions results lay foundation understanding regulatory mechanism provide data future improvement breeding.

Язык: Английский

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Xyloglucan endotransglucosylase-hydrolase 22 positively regulates response to cold stress in upland cotton (Gossypium hirsutum L.)

Industrial Crops and Products, Год журнала: 2024, Номер 220, С. 119273 - 119273

Опубликована: Июль 23, 2024

Язык: Английский

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GhGASA14 regulates the flowering time of upland cotton in response to GA3

Plant Cell Reports, Год журнала: 2024, Номер 43(7)

Опубликована: Июнь 13, 2024

Язык: Английский

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A MADS-box protein GhAGL8 promotes early flowering and increases yield without compromising fiber quality in cotton

Industrial Crops and Products, Год журнала: 2025, Номер 225, С. 120545 - 120545

Опубликована: Янв. 24, 2025

Язык: Английский

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Characterization and Functional Analysis of CONSTANS-like 3 involved in photoperiodic flowering of Gossypium hirsutum

Plant Physiology and Biochemistry, Год журнала: 2025, Номер 221, С. 109643 - 109643

Опубликована: Фев. 12, 2025

Язык: Английский

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Late sowing with reduced nitrogen rate promotes relative maturation and nitrogen use efficiency without compromising yield in cotton

Field Crops Research, Год журнала: 2025, Номер 324, С. 109790 - 109790

Опубликована: Фев. 14, 2025

Язык: Английский

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