Frontiers in Plant Science, Год журнала: 2024, Номер 15
Опубликована: Ноя. 25, 2024
Current eggplant variety breeding is still mainly based on conventional methods, and there remains a lack of effective molecular systems for complex traits controlled by multiple genes, such as yield quality. To accelerate the research progress genetics breeding, it necessary to implement genome-based strategy.
Язык: Английский
Theoretical and Applied Genetics, Год журнала: 2024, Номер 137(9)
Опубликована: Авг. 22, 2024
Язык: Английский
Процитировано
7
BMC Plant Biology, Год журнала: 2025, Номер 25(1)
Опубликована: Фев. 3, 2025
Язык: Английский
Процитировано
0
The Nucleus, Год журнала: 2023, Номер 66(3), С. 341 - 353
Опубликована: Окт. 27, 2023
Язык: Английский
Процитировано
7
Frontiers in Plant Science, Год журнала: 2024, Номер 15
Опубликована: Апрель 26, 2024
High fiber strength (FS) premium cotton has significant market demand. Consequently, enhancing FS is a major objective in breeding quality cotton. However, there notable lack of known functionally applicable genes that can be targeted for breeding. To address this issue, our study used specific length–amplified fragment sequencing combined with bulk segregant analysis to trait an F 2 population. Subsequently, we integrated these results previous quantitative locus mapping regarding quality, which simple sequence repeat markers , 2:3 and recombinant inbred line populations. We identified stable qFS A06 associated located on chromosome (90.74–90.83 Mb). Within interval, cloned gene, GhALDH7B4_A06 harbored critical mutation site coding sequences distinct the two parents tested line. In paternal parent Ji228, gene normal referred as O ; however, nonsense maternal Ji567 premature termination protein translation, designated truncated S . Validation using lines expression revealed correlated FS. Virus-induced silencing GhALDH7B4 caused decreases micronaire. Conversely, overexpression Arabidopsis boosted cell wall component contents stem. The findings provide candidate improving through molecular
Язык: Английский
Процитировано
1
Industrial Crops and Products, Год журнала: 2024, Номер 216, С. 118706 - 118706
Опубликована: Май 11, 2024
Язык: Английский
Процитировано
1
Electronic Journal of Biotechnology, Год журнала: 2024, Номер 70, С. 14 - 22
Опубликована: Май 23, 2024
Cold damage of maize during germination is a global problem, it occurs frequently in northeast China, and leads to large scale reduction yield. Low temperature tolerance complex quantitative trait controlled by multi genes, no major QTLs or key genes have been identified. A F2 isolation population with S319 R144 as parents was constructed. The bulked segregant analysis (BSA) specific-locus amplified fragment-sequencing (SLAF-seq) methods were applied locate the chromosomal association regions related low germination. Sequencing obtained 221.72 Gbp clean data, an average sequencing depth 25.96X. Four candidate associated obtained, total length 25.71 Mb 1513 annotated including 456 nonsynonymous mutant 111 frameshift genes. This study aimed lay foundation for mining germination, accelerate process targeted improvement molecular marker assisted breeding.
Язык: Английский
Процитировано
1
Опубликована: Июнь 25, 2024
Superior fiber quality is one of the most important objectives in cotton breeding. To detect genetic basis underlying quality, an F2 population containing 413 plants was constructed by crossing Jifeng 914 and 173, both which have superior with 173 being better. Five traits were investigated F2, F2:3, F2:4, F2:5 populations. Quantitative trait loci (QTL) mapping conducted based on a high-density map 11,488 single nucleotide polymorphisms (SNPs) spanning 4,202.12 cM length. Transgressive segregation patterns complex correlations five tested observed. A total 108 QTLs found, including 27 for length (FL), 16 strength (FS), 24 micronaire (MC), 17 uniformity (FU), elongation rate (FE). Chromosome A7 contained 12 QTL, ranking first. No QTL found chromosome D1 D11. Each contributed 1.98%–21.45% to phenotypic variation (PV), 13 major effect that more than 10% toward PV. Two could be repeatedly detected three populations, qFL-D3-2 9.18%–21.45% PV, qFS-A11-1 F2:4 6.05%–10.41% Another seven stable two four QTLs: qFL-A12-3, qFS-D10-2, qMC-D6-2, qMC-D8-1. Fourteen QTL-overlapping regions might explain among traits. Four A11, D3, D6, D10 covered are promising further fine mapping. The genomic populations contain 810 genes. Gene functional analysis revealed annotated genes mainly involved protein binding metabolic pathways. Fifteen candidate region highly expressed or ovules during initiation, elongation, secondary cell wall thickening, maturation stages. qRT-PCR Ghir_D03G005440.1 Ghir_D03G011310.1 may play role promoting Ghir_D03G006470.1 beneficial elongation. This study provides information revealing molecular quality.
Язык: Английский
Процитировано
1
Physiologia Plantarum, Год журнала: 2024, Номер 176(4)
Опубликована: Июль 1, 2024
Abstract Cotton plays a crucial role in the progress of textile industry and betterment human life by providing natural fibers. In our study, we explored genetic determinants cotton architecture fiber yield quality crossbreeding Gossypium hirsutum barbadense , creating recombinant inbred line (RIL) population. Utilizing SNP markers, constructed an extensive map encompassing 7,730 markers over 2,784.2 cM. We appraised two architectural seven traits within six environments, identifying 58 QTLs, which 49 demonstrated stability across these environments. These encompassed QTLs for such as lint percentage (LP), boll weight (BW), strength (STRENGTH), seed index (SI), micronaire (MIC), primarily located on chromosomes chr‐A07, chr‐D06, chr‐D07. Notably, chr‐D07 houses QTL region affecting SI, corroborated multiple studies. Within this region, genes BZIP043 SEP2 were identified pivotal, with particularly showing augmented expression developing ovules. discoveries contribute significantly to marker‐assisted selection, potentially elevating both production. findings provide valuable insights into breeding strategies, offering information enhance
Язык: Английский
Процитировано
1
BMC Genomics, Год журнала: 2024, Номер 25(1)
Опубликована: Авг. 24, 2024
As a Brassica crop, napus typically has single flowers that contain four petals. The double-flower phenotype of rapeseed been desirable trait in China because its potential commercial value ornamental tourism. However, few double-flowered germplasms have documented B. napus, and knowledge the underlying genes is limited. Here, D376 was characterized as strain presented an average 10.92 ± 1.40 petals other normal floral organs. F1, F2 BC1 populations were constructed by crossing with single-flowered line reciprocally. Genetic analysis revealed recessive controlled multiple genes. To identify key controlling trait, bulk segregant sequencing (BSA-seq) RNA-seq analyses conducted on individual bulks opposite extreme phenotypes. Through BSA-seq, one candidate interval mapped at region chromosome C05: 14.56–16.17 Mb. GO KEGG enrichment DEGs significantly enriched carbohydrate metabolic processes, notably starch sucrose metabolism. Interestingly, five thirty-six associated development up- down-regulated, respectively, plants. A combined BSA-seq data candidates double flower BnaC05.ERS2 most promising gene. These findings provide novel insights into breeding varieties lay theoretical foundation for unveiling molecular mechanisms napus.
Язык: Английский
Процитировано
1
Euphytica, Год журнала: 2024, Номер 220(10)
Опубликована: Сен. 12, 2024
Язык: Английский
Процитировано
1