Oral Oncology,
Год журнала:
2021,
Номер
120, С. 105404 - 105404
Опубликована: Июль 2, 2021
Oral
microbiome
plays
an
important
role
in
oral
diseases.
Among
them,
proliferative
verrucous
leucoplakia
(PVL)
is
uncommon
form
of
progressive
multifocal
leukoplakia
with
a
worryingly
rate
malignant
transformation.
Here,
we
aimed
to
characterize
the
PVL
patients
and
compare
it
those
healthy
controls.Oral
biopsies
from
ten
five
individuals
were
obtained
used
their
microbial
communities.
The
sequence
V3-V4
region
16S
rRNA
gene
was
as
taxonomic
basis
estimate
analyze
composition
diversity
bacterial
populations
present
samples.Our
results
show
that
are
significantly
different
among
donors.
average
number
observed
operational
units
(OTUs)
higher
for
donors
than
PVL,
proving
loss
PVL.
Several
OTUs
found
be
more
abundant
either
group.
enriched
patients,
potential
protumorigenic
pathogens
like
Oribacterium
sp.
taxon
108,
Campylobacter
jejuni,
uncultured
Eubacterium
sp.,
Tannerella,
Porphyromonas
identified.Oral
dysbiosis
suffering
To
best
our
knowledge,
this
first
study
investigating
alterations
and,
due
limited
participants,
additional
studies
needed.
microbiota-based
biomarkers
may
helpful
predicting
risks
development
Oral Diseases,
Год журнала:
2023,
Номер
30(3), С. 1072 - 1083
Опубликована: Март 9, 2023
Abstract
Objective
Proliferative
verrucous
leukoplakia
(PVL)
has
high
rates
of
malignant
transformation
into
oral
squamous
cell
carcinoma
(OSCC),
but
the
clinical
and
evolutionary
pattern
OSCC
from
PVL
(PVL‐OSCC)
is
more
favorable
than
that
not
preceded
by
(OSCC).
Here,
we
aimed
to
explore
pathophysiologic
differences
between
PVL‐OSCC
through
transcriptomic
DNA
methylation
analyses.
Materials
methods
In
this
case‐control
study,
biopsies
8
10
patients
were
obtained
for
global
sequencing
using
RNAseq
a
genome‐wide
analysis
via
Infinium
EPIC
Platform
(graphical
abstract).
Results
One
hundred
thirty‐three
differentially
expressed
genes
(DEGs)
detected,
94
them
upregulated
in
OSCC.
Most
these
previously
described
cancer
associated
with
prognosis.
The
integrative
revealed
26
DEGs,
corresponding
37
CpGs,
whose
promoters
regulated
methylation.
Twenty‐nine
CpGs
found
as
hypermethylated
PVL‐OSCC.
Only
5
aberrantly
methylated
patients,
whereas
21
underexpressed.
Conclusions
presented
lower
expression
cancer‐related
genes.
Hypermethylation
promoter
region
many
was
also
noticed,
indicating
could
be
regulatory
mechanism.
Proliferative
verrucous
leukoplakia
(PVL),
distinguished
by
its
malignant
transformation
rate
of
43.87%
to
65.8%,
stands
as
the
oral
potentially
disorder
with
highest
propensity
for
malignancy.
PVL
is
marked
distinctive
heterogeneity
regarding
clinical
or
histopathological
characteristics
well
prognostic
factors
pertinent
this
condition.
The
purpose
study
compile
and
assess
clinicopathological
features,
transformation,
associated
risk
in
patients
diagnosed
PVL.
Cancers,
Год журнала:
2021,
Номер
13(15), С. 3669 - 3669
Опубликована: Июль 21, 2021
Proliferative
verrucous
leukoplakia
(PVL)
is
considered
as
an
oral
potentially
malignant
disorder
(OPMD)
that
presents
with
a
high
tendency
to
recurrence
after
treatment
and
has
the
highest
transformation
ratio
among
all
OPMD
(50%).
Evidence-based
publications
have
indicated
evolution
reported
significantly
related
inconsistent
diagnostic
criteria
used
in
primary-level
studies;
so,
it
been
hypothesized
risk
of
cancer
for
this
disease
could
even
be
underestimated.
This
important
because
PVL
requires
specific
management
protocols,
evidence-based,
aimed
at
early
diagnosis
developing
these
lesions.
We
present
scoping
review-a
novel
approach
mapping
available
literature
on
given
topic
provide
overview
research
evidence
highlight
possible
gaps
evidence-especially
our
study
aspects
PVL,
issue
conceptual
proposal
PVL.
conclude
white,
multifocal
progressive
lesion
rate
which
diagnosed
mainly
around
age
60
years
without
any
histological
characterization.
also
advise
personal
reflection
level
certainty
clinician
makes
particular
case
Oral Oncology,
Год журнала:
2021,
Номер
120, С. 105404 - 105404
Опубликована: Июль 2, 2021
Oral
microbiome
plays
an
important
role
in
oral
diseases.
Among
them,
proliferative
verrucous
leucoplakia
(PVL)
is
uncommon
form
of
progressive
multifocal
leukoplakia
with
a
worryingly
rate
malignant
transformation.
Here,
we
aimed
to
characterize
the
PVL
patients
and
compare
it
those
healthy
controls.Oral
biopsies
from
ten
five
individuals
were
obtained
used
their
microbial
communities.
The
sequence
V3-V4
region
16S
rRNA
gene
was
as
taxonomic
basis
estimate
analyze
composition
diversity
bacterial
populations
present
samples.Our
results
show
that
are
significantly
different
among
donors.
average
number
observed
operational
units
(OTUs)
higher
for
donors
than
PVL,
proving
loss
PVL.
Several
OTUs
found
be
more
abundant
either
group.
enriched
patients,
potential
protumorigenic
pathogens
like
Oribacterium
sp.
taxon
108,
Campylobacter
jejuni,
uncultured
Eubacterium
sp.,
Tannerella,
Porphyromonas
identified.Oral
dysbiosis
suffering
To
best
our
knowledge,
this
first
study
investigating
alterations
and,
due
limited
participants,
additional
studies
needed.
microbiota-based
biomarkers
may
helpful
predicting
risks
development
