Genetic Variation in Brassicaceae unlocks Diversity for Enhanced Glucosinolate Metabolism

Vegetable Research, Год журнала: 2024, Номер 4(1), С. 0 - 0

Опубликована: Янв. 1, 2024

Glucosinolates are a class of secondary metabolites derived from amino acids that widely distributed in Brassicaceae family plants such as cabbage and broccoli. An S-β-d-glucopyrano unit is anomerically linked to an O-sulfated (Z)-thiohydroximate moiety form glucosinolates. Despite the biological inactivity intact glucosinolates, action myrosinase on glucosinolate generates glucosinolates hydrolysis products, which increase tolerance abiotic stress, biotic stress improve human health. Here, we investigate possible applications bioactive functions, with aim harnessing them for advancement sustainable agriculture future. In order obtain advanced sources various methods have been utilized, traditional breeding, transgenic techniques, hairy root plant cell cultures, microbial host-based engineering, biotechnological applied Brassica crops. We can examine assess possibility manipulating genes related biosynthesis, hydrolysis, transport currently either underutilized or exploited, considering potential targets GSL engineering. This synthesis aims contribute deeper understanding intricate relationships within glucosinolate-related processes their mitigation strategies plants. conclusion, this study provides comprehensive insight into genetic diversity its influence metabolism. It opens up new avenues targeted breeding crops enhanced nutritional ecological benefits, underlining importance agricultural sustainability.

Язык: Английский

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Advancements in balancing glucosinolate production in plants to deliver effective defense and promote human health

Agriculture Communications, Год журнала: 2024, Номер 2(2), С. 100040 - 100040

Опубликована: Май 16, 2024

Glucosinolates (GSLs) are a prototypical group of bioactive compounds found in the Brassicaceae family that promote human health and plant defense. The GSL-myrosinase system can be induced to release multiple products when plants subjected mechanical damage, environmental stress, or pathogen infection. While many GSLs health, some cause deleterious effects ingested. To engineer crops with lower levels harmful without sacrificing health-promoting requires complete understanding origin advances GSL modification. Extensive early domestication studies were conducted using classic breeding nutrition. More recently, genetic modification specific groups tissues has been partially successful. However, efforts have fallen short delivering reduction potentially concomitant losses latest work synthetically express biosynthesis pathways non-host microbial species. yields far from economically sustainable. This review discusses key made promising for precise content composition optimal defense health.

Язык: Английский

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Studying Salt-Induced Shifts in Gene Expression Patterns of Glucosinolate Transporters and Glucosinolate Accumulation in Two Contrasting Brassica Species

Metabolites, Год журнала: 2024, Номер 14(4), С. 179 - 179

Опубликована: Март 22, 2024

Brassica crops are well known for the accumulation of glucosinolates-secondary metabolites crucial plants' adaptation to various stresses. Glucosinolates also functioning as defence compounds pose challenges food quality due their goitrogenic properties. Their disruption leaves plants susceptible insect pests and diseases. Hence, a targeted reduction in seed glucosinolate content is paramount importance increase acceptance. GLUCOSINOLATE TRANSPORTERS (GTRs) present promising avenue selectively reducing concentrations seeds while preserving biosynthesis elsewhere. In this study, 54 putative GTR protein sequences found were retrieved, employing Arabidopsis GTR1 GTR2 templates. Comprehensive bioinformatics analyses, encompassing gene structure organization, domain analysis, motif assessments, promoter cis-regulatory elements, affirmed existence transporter domains stress-related regulatory elements. Phylogenetic analysis revealed patterns conservation divergence across species. have been shown under stress conditions, indicating potential role response. To elucidate GTRs transportation NaCl two distinct species, B. juncea napus, subjected 0, 100, or 200 mM NaCl. Based on literature, key genes chosen expression plant parts was assessed. Both species displayed divergent trends biochemical profiles contents elevated salt conditions. Statistical modelling identified significant contributors variations, guiding development breeding strategies low-glucosinolate varieties. Notably, GTR2A2 exhibited pronounced expressions stems, contributing approximately 52% variance, GTR2B1/C2 flowers. Additionally, GTR2A1 GTR1A2/B1 demonstrated noteworthy roots. This study enhances our understanding regulation offering avenues improve crop resilience.

Язык: Английский

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Breeding and biotechnology approaches to enhance the nutritional quality of rapeseed byproducts for sustainable alternative protein sources- a critical review

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Ноя. 11, 2024

Global protein consumption is increasing exponentially, which requires efficient identification of potential, healthy, and simple sources to fulfil the demands. The existing animal proteins are high in fat low fiber composition, might cause serious health risks when consumed regularly. Moreover, production from can negatively affect environment, as it often more energy natural resources contributes greenhouse gas emissions. Thus, finding alternative plant-based becomes indispensable. Rapeseed an important oilseed crop world's third leading oil source. byproducts, such seed cakes or meals, considered best source after soybean owing their promising profile (30%-60% crude protein) supplement dietary requirements. After extraction, these rapeseed byproducts be utilized food for human feed. However, anti-nutritional factors (ANFs) like glucosinolates, phytic acid, tannins, sinapines make them unsuitable direct consumption. Techniques microbial fermentation, advanced breeding, genome editing improve quality, reduce ANFs facilitate usage feed industry. This review summarizes approaches offers bio-nutrition breakthroughs develop nutrient-rich sources.

Язык: Английский

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Crop genome editing through tissue-culture-independent transformation methods

Frontiers in Genome Editing, Год журнала: 2024, Номер 6

Опубликована: Дек. 5, 2024

Genome editing and plant transformation are crucial techniques in biotechnology, allowing for the precise modification of genomes to enhance agronomically essential traits. The advancement CRISPR-based genome tools plants is limited, among others, by developing novel

Язык: Английский

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Artificial Fluorescent Glucosinolates (F-GSLs) Are Transported by the Glucosinolate Transporters GTR1/2/3

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(2), С. 920 - 920

Опубликована: Янв. 4, 2023

The glucosinolate transporters 1/2/3 (GTR1/2/3) from the Nitrate and Peptide transporter Family (NPF) play an essential role in transport, accumulation, distribution of specialized plant metabolite glucosinolates. Due to representing both antinutritional health-promoting compounds, there is increasing interest characterizing GTRs various species. We generated seven artificial glucosinolates (either aliphatic or benzenic) bearing different fluorophores (Fluorescein, BODIPY, Rhodamine, Dansylamide, NBD) investigated ability GTR1/2/3 Arabidopsis thaliana import fluorescent (F-GSLs) into oocytes Xenopus laevis. Five out F-GSLs synthesized were imported by at least one GTRs. GTR1 GTR2 able three actively above external concentration, while GTR3 only actively. Competition assays indicate that are transported same mechanism as non-tagged natural GTR-mediated F-GSL uptake detected via a rapid sensitive assay requiring simple fluorescence measurements on standard plate reader. This highly useful investigations transport function provides critical prerequisite for elucidating relationship between structure through high-throughput screening GTR mutant libraries. themselves may also be suitable future studies vivo.

Язык: Английский

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Multi-omic analyses unveil temporal and spatial distribution of specialized metabolites in seeds ofCamelina sativa

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 6, 2024

Abstract Seeds of Brassicaceae species produce a large diversity specialized metabolites (SMs) that strongly influence their quality, with beneficial or toxic effects on human and animal nutrition, provide resistance to biotic abiotic stresses. While the distribution these compounds has been described in leaves roots tissues, very limited information is available about spatio-temporal accumulation seeds model crop plants. Camelina sativa (camelina) an oilseed cultivated for industrial uses. we previously explored detail SM plasticity, no expression SM-related proteins genes camelina seeds. In this study used untargeted metabolomics (LC-MS/MS), proteomics (DIA) transcriptomics (RNA-Seq) analyse synthesis, transport, modifications degradations SMs are accumulated different seed tissues (i.e. coat, endosperm, embryo) at 6 developmental 2 germination stages. Our results showed specific patterns many SMs, related genes, during coat embryo development. We also that, differently from Arabidopsis thaliana seeds, defence antinutritional glucosinolates were both corresponding degradation products isothiocyanates present high level embryos dry C. . Characterizing spatial dynamics will contribute development crops optimized quality nutrition.

Язык: Английский

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Boosting Salt Tolerance in Camelina cultivars through synergistic Impact of Exogenous Silicon and Selenium by modulating physiological attributes and Antioxidant activities

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Июнь 13, 2024

Salt stress (SS) has emerged as one of the most pressing environmental threats to production sustainability oil-seed crops like camelina which necessitates finding out pro-environment and biologically feasible amelioration approaches. A study was performed investigate effects exogenously applied silicon (Si) selenium (Se) on two varieties (Australian Canadian) under SS conditions. The trial comprised doses Si Se (5 10 ppm each) along with co-application treatments entailing (5pp) + (5ppm) (10 pp) ppm), whereas control were kept for comparison purpose. response variables included root-shoot length, fresh dry weights, well leaf photosynthetic pigment content (chlorophyll a, chlorophyll b, total chlorophyll, carotenoids), gas exchange attributes, biochemical characters (total proline, soluble sugar, protein, free amino acid) antioxidant activities (APX, POD, CAT, SOD). Results showed that significantly reduced all growth attributes camelina, Australian variety demonstrated greater salinity tolerance in Canadian variety. ppm) outperformed rest by recording highest a (1.69 mg/g FW), b (1.02 carotenoids (2.89 rate (17.02 µmole CO₂ m^{− 2} s^{− 1}), transpiration (3.65 stomatal conductance (0.38 proline (199.38 µmole/g DW), sugar (120.95 protein (12.02 DW) acid (37.15 compared camelina. same treatment also remained effective triggering activity APX (3.89 Unit/min/g CAT (165.19 POD (178.95 SOD (237.63 FW). Thus, combined exogenous application holds bright perspectives alleviating deleterious impact especially variety, could be recommended growers after thorough field investigations.

Язык: Английский

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Identification of clusters of secondary metabolite biosynthetic genes in the Camelina sativa genome

Natural Product Research, Год журнала: 2024, Номер unknown, С. 1 - 7

Опубликована: Авг. 5, 2024

Multidrug-resistant pathogens pose an earnest risk to human health. Therefore, new antibiotics need be developed quickly. Most of the we use today are derived from secondary metabolites, which produced by plants. Genome mining tools allow us detect biosynthetic gene clusters (BGCs) responsible for production metabolites. Focusing on most promising BGCs-coding with unique pathways is currently a challenge.

Язык: Английский

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Accumulation of Anthocyanin in the Aleurone of Barley Grains by Targeted Restoration of the MYC2 Gene

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12705 - 12705

Опубликована: Ноя. 26, 2024

Blue barley grain pigmentation results from anthocyanin accumulation in the aleurone layer. Anthocyanins are known for their beneficial effects on human health. The gene encoding MYELOCYTOMATOSIS 2 (MYC2) transcription factor is potentially responsible blue coloration of aleurone. In non-pigmented barley, a single nucleotide insertion this causes frameshift mutation with premature stop codon. It was hypothesized that restoring MYC2 reading frame could activate Using targeted mutagenesis approach present study, restored cultivar Golden Promise. Genetic constructs harboring cas9 and gRNA expression units were developed, pre-validated protoplasts, then functional alleles generated at plant level via Agrobacterium-mediated transformation. Anthocyanin layer grains these mutants confirmed through microscopy chemical analysis. biosynthesis genes analyzed, revealing restoration led to increased transcript levels F3H ANS genes. These confirm critical role trait provide biotechnological solution enriching anthocyanins.

Язык: Английский

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