Comparative transcriptome analysis to reveal the genes and pathways associated with adaptation strategies in two different populations of Manila clam (Ruditapes philippinarum) under acute temperature challenge

Aquaculture, Год журнала: 2022, Номер 552, С. 737999 - 737999

Опубликована: Фев. 5, 2022

Язык: Английский

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Retrospect of fish meal substitution in Pacific white shrimp (Litopenaeus vannamei) feed: Alternatives, limitations and future prospects

Reviews in Aquaculture, Год журнала: 2023, Номер 16(1), С. 382 - 409

Опубликована: Июнь 27, 2023

Abstract Pacific white shrimp ( Litopenaeus vannamei ), the most cultured crustacean in world, has become one of key species international aquatic trade accounting for 11.96% total aquaculture production value 2020. The development aquafeed industry, especially large‐scale use fish meal (FM) and oil, contributed to promoting expansion production. However, supply FM fluctuated at about 5 million tons per year affected by El Nino during last decades. This finite combination with continued demand contributes high prices, prompting alternative protein sources. review appropriate replacement strategies feed summarises status research on sources, including plant, animal single‐cell proteins commercial feeds shrimp. Furthermore, several aspects nutritional balances were mentioned amino acid profiles, fatty nutrients trace elements low diets as well some easily overlooked concepts that deserve attention such epigenetics, processing characteristics, growth stages, improvement. Also, prospects future are proposed application advanced technologies (16S rRNA sequencing, transcriptomics, proteomics metabolomics), developments new additives, along optimisation sources establishment a precise programming

Язык: Английский

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A review of the quantitative real-time PCR and Omics approaches applied to study the effects of dietary selenium nanoparticles (nano-Se) on fish

Comparative Immunology Reports, Год журнала: 2024, Номер 6, С. 200127 - 200127

Опубликована: Янв. 12, 2024

Selenium (Se) is an essential trace microelement required for the overall health of humans and animals. The importance Se mainly related to its participation in structure selenoproteins with diverse biological functions, including antioxidant defense, immunity, thyroid hormone metabolism. functionality depends on chemical form (inorganic organic Se). Due low toxicity higher efficacy, nanoparticles (nano-Se) have been recently applied aquafeeds enhance fish performance. New technological advances offered different Omics approaches, such as transcriptomics, proteomics, metabolomics, realize molecular mechanisms underlying processes. In recent years, approaches employed study nano-Se effects fish. present article summarizes impacts supplementation performance, then reviews qRT-PCR assay Omics-based used dietary

Язык: Английский

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Integration of Omics Tools for Understanding the Fish Immune Response Due to Microbial Challenge

Frontiers in Marine Science, Год журнала: 2021, Номер 8

Опубликована: Июнь 21, 2021

Nowadays, fish production through aquaculture and wild capture is escalating due to the higher protein demand for human consumption. Thus, needs products be improved in scale quality are becoming increasingly important meet nutrition requirements. The negative impacts caused by pathogens infection both hatchery grow-out ponds, have resulted low productivity economic losses farmers. Therefore, a systematic study exploring relevant omics datasets systems biology approach will enable scientist describe complexity characteristics of interactions host-pathogen network, leading identifying new targets or biomarkers diseases bacteria, viruses, parasites. use different approaches emerging technologies as powerful tool obtaining knowledge sustainable has been applied many researchers over years. In this paper, several used assess immune response were reviewed. A brief description provided applicable aquacultures, such genomics, transcriptomics, proteomics, metabolomics. Each contributes develop better treatment prevention strategies overcome disease microbial infections. genomics transcriptomics analysis, their parallel involving gene expression studies play role immune-related genes host, infectious diseases. While proteomics metabolomics approaches, these provide information on biomarker discovery understanding biosynthetic pathway during pathogen infection. Additionally, examples integrative analysis multi-omics tools required understand biological aquatic animals especially fish, also provided.

Язык: Английский

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Proteomics in fish health and aquaculture productivity management: Status and future perspectives

Aquaculture, Год журнала: 2022, Номер 566, С. 739159 - 739159

Опубликована: Дек. 15, 2022

Язык: Английский

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Single cell genomics as a transformative approach for aquaculture research and innovation

Reviews in Aquaculture, Год журнала: 2023, Номер 15(4), С. 1618 - 1637

Опубликована: Март 7, 2023

Abstract Single cell genomics encompasses a suite of rapidly maturing technologies that measure the molecular profiles individual cells within target samples. These approaches provide large up‐step in biological information compared to long‐established ‘bulk’ methods profile average all sample, and have led transformative advances understanding cellular biology, particularly humans model organisms. The application single is fast expanding non‐model taxa, including aquaculture species, where numerous research applications are underway with many more envisaged. In this review, we highlight potential research, considering barriers solutions broad uptake these technologies. Focusing on transcriptomics, outline considerations for experimental design, essential requirement obtain high quality cells/nuclei sequencing ectothermic aquatic species. We further data analysis bioinformatics considerations, tailored studies under‐characterized genomes our knowledge heterogeneity marker genes immature. Overall, review offers useful source researchers aiming apply address challenges faced by global sector though an improved biology.

Язык: Английский

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Dietary antarctic krill improves antioxidant capacity, immunity and reduces lipid accumulation, insights from physiological and transcriptomic analysis of Plectropomus leopardus

BMC Genomics, Год журнала: 2024, Номер 25(1)

Опубликована: Фев. 26, 2024

Abstract Background Due to its enormous biomass, Antarctic krill ( Euphausia superba ) plays a crucial role in the Ocean ecosystem. In recent years, has found extensive application aquaculture, emerging as sustainable source of aquafeed with ideal nutritional profiles. However, comprehensive study focused on detailed effects dietary aquaculture animals, especially farmed marine fishes, is yet be demonstrated. Results this study, comparative experiment was performed using juvenile P. leopardus , fed diets supplemented (the group) or without control group). Histological observation revealed that could reduce lipid accumulation liver while intestine exhibited no obvious changes. Enzyme activity measurements demonstrated had an inhibitory effect oxidative stress both and liver. By transcriptome analysis, total 1,597 1,161 differentially expressed genes (DEGs) were identified liver, respectively. Functional analysis DEGs showed multiple enriched terms significantly related cholesterol metabolism, antioxidants, immunity. Furthermore, expression profiles representative DEGs, such dhcr7 apoa4 sc5d scarf1 validated by qRT-PCR fluorescence situ hybridization. Finally, demonstrate biased astaxanthin . Conclusions Our enhance antioxidant capacities exhibit molecular-level improvements immunity, antioxidants. It will contribute understanding protective provide insights into strategies.

Язык: Английский

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Tracking Biomarkers for the Health and Welfare of Aquaculture Fish

Fishes, Год журнала: 2024, Номер 9(7), С. 289 - 289

Опубликована: Июль 19, 2024

Aquaculture production has been growing consistently over the last few decades to meet increasing animal protein demand of human population. However, increased and rearing intensities raise challenges guaranteeing fish health welfare, which is essential avoid losses ensure product quality. Biomarkers can provide insights into fish’s nutritional, physiological, status, aid in evaluation early nutritional physiological imbalances, distress conditions, pathological diagnosis. The discovery validation biomarkers rely mostly on use information provided by different parameters, including biochemical, metabolic, or immunologic, as well several omics, from genomics transcriptomics proteomics metabolomics. In this review, a summary main used aquaculture along with an overview omics technologies available for further biomarker research. This review also highlights need develop non-lethal that easily quickly be measured prompt response producers.

Язык: Английский

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Multi-omics integrative analysis reveals the molecular mechanisms of muscle adaptive changes in largemouth bass (Micropterus salmoides) under water flow stress in recirculating aquaculture

Aquaculture, Год журнала: 2025, Номер unknown, С. 742172 - 742172

Опубликована: Янв. 1, 2025

Язык: Английский

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Dietary Tannic Acid Promotes Growth Performance and Resistance Against Aeromonas hydrophila Infection by Improving the Antioxidative Capacity and Intestinal Health in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Antioxidants, Год журнала: 2025, Номер 14(1), С. 112 - 112

Опубликована: Янв. 20, 2025

To investigate the effect of tannic acid (TA) on growth, disease resistance, and intestinal health Chinese soft-shelled turtles, individual turtles were fed with 0 g/kg (CG), 0.5 g/kg, 1 2 4 TA diets for 98 days. Afterwards, turtles’ resistance was tested using Aeromonas hydrophila. The results showed that 0.5–4 dietary increased growth performance feed utilization (p < 0.05), 2.38 being optimal level specific rate (SGR). addition in mucosal fold height submucosa thickness small intestine, which reached a maximum g/kg. 0.5–2 effectively reduced cumulative mortality had been induced by A. hydrophila, dosage leading to lowest mortality. Additionally, 1–4 improved T-SOD, CAT, GSH-Px activities during infection, while enhanced richness diversity microbiota, example, increasing Actinobacteria but inhibiting Firmicutes. transcriptome demonstrated predominant differentially expressed genes (DEGs) TA2 mainly enriched PPAR signaling pathway (Acsl5, Apoa2, Apoa5, Fabp1, Fabp2, Fabp6); glycine, serine, threonine metabolism (Chdh, Gatm, Shmt1); steroid biosynthesis (Cel, Hsd17b7, Soat2, Sqle). main metabolites (DEMs) discovered means metabolome analysis included cholylhistidine, calcipotriol, 13-O-tetradecanoylphorbol 12-acetate, hexahomomethionine CG vs. TA2. Integrative analyses two omics revealed mitigated inflammation activating regulating lipid via multiple pathways, such as α-linolenic metabolism. In general, inclusion turtle can optimally promote bacterial maintaining improving antioxidant capacity.

Язык: Английский

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