OMICs, Epigenetics, and Genome Editing Techniques for Food and Nutritional Security DOI Creative Commons
Yuri Gogolev, Sunny Ahmar, Bala Anı Akpınar

и другие.

Plants, Год журнала: 2021, Номер 10(7), С. 1423 - 1423

Опубликована: Июль 12, 2021

The incredible success of crop breeding and agricultural innovation in the last century greatly contributed to Green Revolution, which significantly increased yields ensures food security, despite population explosion. However, new challenges such as rapid climate change, deteriorating soil, accumulation pollutants require much faster responses more effective solutions that cannot be achieved through traditional breeding. Further prospects for increasing efficiency agriculture are undoubtedly associated with inclusion strategy knowledge obtained using high-throughput technologies tools future ensure design plant genomes predict desired phenotype. This article provides an overview current state research these areas, well study soil microbiomes, prospective use their potential a field microbiome engineering. In terms genomic phenomic predictions, we also propose integrated approach combines high-density genotyping phenotyping techniques, can improve prediction accuracy quantitative traits species.

Язык: Английский

CRISPR/Cas genome editing in soybean: Challenges and new insights to overcome existing bottlenecks DOI Creative Commons

Nayara S. Freitas-Alves,

Clídia E. Moreira-Pinto,

Fabiano T. P. K. Távora

и другие.

Journal of Advanced Research, Год журнала: 2024, Номер unknown

Опубликована: Авг. 1, 2024

Soybean is a worldwide-cultivated crop due to its applications in the food, feed, and biodiesel industries. Genome editing soybean began with ZFN TALEN technologies; however, CRISPR/Cas has emerged shortly became preferable approach for genome manipulation since it more precise, easy handle, cost-effective. Recent reports have focused on conventional Cas9 nuclease, nickase (nCas9) derived base editors, Cas12a (formally Cpf1) as most commonly used editors soybean. Nonetheless, several challenges complex plant genetic engineering pipeline need be overcome effectively edit of an elite cultivar. These include (1) optimizing CRISPR cassette design (i.e., gRNA Cas promoters, testing, number gRNAs, binary vector), (2) improving transformation frequency, (3) increasing efficiency ratio targeted cells, (4) production.

Язык: Английский

Процитировано

6
EDS1 complexes are not required for PRR responses and execute TNL‐ETI from the nucleus in Nicotiana benthamiana DOI Creative Commons
Josua Zönnchen, Johannes Gantner, Dmitry Lapin

и другие.

New Phytologist, Год журнала: 2022, Номер 236(6), С. 2249 - 2264

Опубликована: Сен. 24, 2022

Heterodimeric complexes incorporating the lipase-like proteins EDS1 with PAD4 or SAG101 are central hubs in plant innate immunity. functions encompass signal relay from TIR domain-containing intracellular NLR-type immune receptors (TNLs) towards RPW8-type helper NLRs (RNLs) and, Arabidopsis thaliana, bolstering of signaling and resistance mediated by cell-surface pattern recognition (PRRs). Increasing evidence points to activation small molecule binding. We used CRISPR/Cas-generated mutant lines agroinfiltration-based complementation assays interrogate Nicotiana benthamiana. did not detect impaired PRR N. benthamiana deficient RNLs. Intriguingly, monitoring SlEDS1-NbEDS1 benthamiana, mutations within SlEDS1 catalytic triad could abolish enhance TNL Furthermore, nuclear accumulation was sufficient for (Roq1) Reinforcing might be a derived function TNL/EDS1 sector. Although Solanaceae functionally depends on residues some contexts, our data do support binding TNL-derived environment. Whether how activity connects membrane pore-forming RNLs remains unknown.

Язык: Английский

Процитировано

27
Effector XopQ-induced stromule formation inNicotiana benthamianadepends on ETI signaling components ADR1 and NRG1 DOI Creative Commons
Jennifer Prautsch, Jessica L. Erickson, Sedef Özyürek

и другие.

PLANT PHYSIOLOGY, Год журнала: 2022, Номер 191(1), С. 161 - 176

Опубликована: Окт. 19, 2022

In Nicotiana benthamiana, the expression of Xanthomonas effector XANTHOMONAS OUTER PROTEIN Q (XopQ) triggers RECOGNITION OF XOPQ1 (ROQ1)-dependent effector-triggered immunity (ETI) responses accompanied by accumulation plastids around nucleus and formation stromules. Both plastid clustering stromules were proposed to contribute ETI-related hypersensitive cell death thereby plant immunity. Whether these reactions are directly connected ETI signaling events has not been tested. Here, we utilized transient experiments determine whether XopQ-triggered a result XopQ perception immune receptor ROQ1 or consequence virulence activity. We found that N. benthamiana mutants lacking ROQ1, ENHANCED DISEASE SUSCEPTIBILITY 1, helper NUCLEOTIDE-BINDING LEUCINE-RICH REPEAT IMMUNE RECEPTORS (NLRs) N-REQUIRED GENE 1 (NRG1) ACTIVATED RESISTANCE (ADR1), fail elicit XopQ-dependent host stromule formation. Mutants only NRG1 lost but retained some induction was abolished in nrg1_adr1 double mutant. This analysis aligns with cascade programmed death. Furthermore, data reveal is strictly linked during ETI. Our suggest formation, contrast chloroplast perinuclear dynamics, an integral part response both ADR1 hNLRs play role this response.

Язык: Английский

Процитировано

23
Current Advancements and Limitations of Gene Editing in Orphan Crops DOI Creative Commons

Matthew Venezia,

Kate M. Creasey Krainer

Frontiers in Plant Science, Год журнала: 2021, Номер 12

Опубликована: Сен. 22, 2021

Gene editing provides precise, heritable genome mutagenesis without permanent transgenesis, and has been widely demonstrated applied in planta . In the past decade, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) revolutionized application of gene crops, with mechanistic advances expanding its potential, including prime base editing. To date, CRISPR/Cas utilized over a dozen orphan crops diverse genetic backgrounds, leading to novel alleles beneficial phenotypes for breeders, growers, consumers. conjunction adoption science-based regulatory practices, there is potential CRISPR/Cas-mediated crop improvement programs solve plethora agricultural problems, especially impacting developing countries. Genome sequencing progressed, becoming more affordable applicable crops. Open-access resources allow target identification guide RNA (gRNA) design evaluation, modular cloning systems enzyme screening methods providing experimental feasibility. While genomic limitations are being overcome, transformation regeneration continue be bottleneck applications. International collaboration between all stakeholders involved vital provide equitable access bridge scientific gap world’s most economically important under-researched This review describes mechanisms workflow addresses challenges, current applications, future prospects

Язык: Английский

Процитировано

31
OMICs, Epigenetics, and Genome Editing Techniques for Food and Nutritional Security DOI Creative Commons
Yuri Gogolev, Sunny Ahmar, Bala Anı Akpınar

и другие.

Plants, Год журнала: 2021, Номер 10(7), С. 1423 - 1423

Опубликована: Июль 12, 2021

The incredible success of crop breeding and agricultural innovation in the last century greatly contributed to Green Revolution, which significantly increased yields ensures food security, despite population explosion. However, new challenges such as rapid climate change, deteriorating soil, accumulation pollutants require much faster responses more effective solutions that cannot be achieved through traditional breeding. Further prospects for increasing efficiency agriculture are undoubtedly associated with inclusion strategy knowledge obtained using high-throughput technologies tools future ensure design plant genomes predict desired phenotype. This article provides an overview current state research these areas, well study soil microbiomes, prospective use their potential a field microbiome engineering. In terms genomic phenomic predictions, we also propose integrated approach combines high-density genotyping phenotyping techniques, can improve prediction accuracy quantitative traits species.

Язык: Английский

Процитировано

28