Plants,
Год журнала:
2025,
Номер
14(4), С. 504 - 504
Опубликована: Фев. 7, 2025
The
TaVP1-B
gene,
located
on
the
3B
chromosome
of
wheat,
is
a
homolog
Viviparous-1
(VP-1)
gene
maize
and
was
reported
to
confer
resistance
pre-harvest
sprouting
(PHS)
in
wheat.
In
this
study,
structure
analyzed
using
wheat
pan-genome
consisting
20
released
cultivars
(19
are
from
China),
3
single
nucleotide
polymorphisms
(SNPs),
which
were
identified
at
496
bp,
524
1548
bp
CDS
region,
respectively.
Haplotypes
analysis
showed
that
these
SNPs
complete
linkage
disequilibrium
only
two
haplotypes
designated
as
hap1
(TGG)
hap2
(GAA)
present.
Association
between
PHS
four
experiment
environments
revealed
average
accessions
with
significantly
better
than
hap2,
infers
effects
resistance.
To
further
investigate
impacts
alleles
locus
resistance,
SNP
region
converted
KASP
marker,
used
for
genotyping
304
Chinese
cultivars,
whose
evaluated
three
environments.
rates
(SRs)
135
lower
169
validating
present
study
provided
breeding-friendly
marker
functional
variants
can
be
genetic
improvement
Frontiers in Plant Science,
Год журнала:
2024,
Номер
14
Опубликована: Янв. 8, 2024
The
significance
of
heat
stress
in
agriculture
is
ever-increasing
with
the
progress
global
climate
changes.
Due
to
a
negative
effect
on
yield
staple
crops,
including
wheat,
impairment
plant
reproductive
development
triggered
by
high
ambient
temperature
became
restraint
food
production.
Although
sensitivity
male
meiosis
and
following
gamete
wheat
has
long
been
recognized,
detailed
structural
characterization
combined
comprehensive
gene
expression
analysis
not
done
about
this
phenomenon.
We
demonstrate
here
that
severely
alters
cytoskeletal
configuration,
triggers
failure
meiotic
division
wheat.
Moreover,
it
changes
genes
related
meiocytes
tapetum
layer
genotype-dependent
manner.
‘Ellvis’,
heat-tolerant
winter
cultivar,
showed
spikelet
fertility
rate
only
scarce
aberrations
upon
exposure
temperature.
In
addition,
shock
involved
scavenging
reactive
oxygen
species
were
significantly
upregulated
meiosis-specific
major
developmental
stability
cultivar.
heat-sensitive
‘Mv
17-09’,
however,
participating
fiber
nucleation,
spindle
assembly
checkpoint
genes,
tapetum-specific
regulators
downregulated.
These
alterations
may
be
decreased
cytoskeleton
content,
frequent
micronuclei
formation,
erroneous
persistence
observed
sensitive
genotype.
Our
results
suggest
understanding
regulation
these
functions
would
an
essential
contribution
new,
cultivars.
Abstract
Leaf
rust,
caused
by
Puccinia
triticina
(
Pt
),
is
a
serious
constraint
to
wheat
production.
Developing
resistant
varieties
the
best
approach
managing
this
disease.
Wheat
leaf
rust
resistance
Lr
)
genes
have
been
classified
into
either
all‐stage
(ASR)
or
adult‐plant
(APR).
The
objectives
of
study
were
identify
sources
in
contemporary
US
hard
winter
(HWW)
and
dissect
genetic
basis
underlying
HWW.
A
panel
732
elite
HWW
genotypes
was
evaluated
for
response
races
at
seedling
stage
adult
plant
nurseries
Oklahoma,
Texas,
Kansas.
Further,
genotyped
using
multiplex
restriction
amplicon
sequencing
(MRA‐Seq)
DNA
markers
linked
known
ASR
Lr18
,
Lr19
Lr21
Lr24
Lr37
Lr42
APR
Lr34
Lr46
Lr67
Lr68
Lr77
Lr78
.
Single
nucleotide
polymorphism
(SNP)
derived
from
MRA‐Seq,
genes,
phenotypic
data
used
genome‐wide
association
(GWAS)
associated
with
response.
Gene
postulation
based
on
reactions,
markers,
GWAS
suggested
presence
Lr1
Lr2a
Lr10
Lr14a
Lr16
Lr26
Lr39
panel.
identified
59
SNPs
significantly
response,
which
20
likely
novel
loci
can
be
enhance
resistance.
Agronomy,
Год журнала:
2025,
Номер
15(1), С. 169 - 169
Опубликована: Янв. 11, 2025
A
chromosome
segment
substituted
line
(CSSL)
represents
an
ideal
resource
for
studying
quantitative
traits
like
thermotolerance.
To
develop
wheat
inter-varietal
CSSLs
with
E6015-3S
(a
heat-sensitive
genotype)
being
the
recipient
parent,
genome-wide
unique
DNA
markers
are
urgently
needed
marker-assisted
selection.
In
this
study,
11,016
primer
pairs
targeting
5036
indel
sites
were
successfully
designed
E6015-3S,
average
density
of
0.36
indels
per
Mbp.
These
believed
to
be
and
polymorphic
in
genome;
as
gathered
from
evidence,
(i)
76.18
99.34%
yielded
a
single
hit
during
sequence
alignment
18
sequenced
genomes,
(ii)
83.59
90.98%
1042
synthesized
amplified
band
16
accessions,
(iii)
59.69
99.81%
tested
between
15
individual
accessions.
also
anticipated
excellent
resolvability
on
agarose
or
polyacrylamide
gels,
since
most
them
have
sizes
46
bp,
amplicon
141
250
polymorphism
ratios
6.0
25.0%.
Collectively,
these
CSSL
development
more
broad
applications,
germplasm
classification,
seed
purity
testing,
genetic
linkage
mapping,
breeding
wheat,
owing
their
uniqueness,
polymorphism,
easy-to-use
characteristics.
Plants,
Год журнала:
2025,
Номер
14(4), С. 504 - 504
Опубликована: Фев. 7, 2025
The
TaVP1-B
gene,
located
on
the
3B
chromosome
of
wheat,
is
a
homolog
Viviparous-1
(VP-1)
gene
maize
and
was
reported
to
confer
resistance
pre-harvest
sprouting
(PHS)
in
wheat.
In
this
study,
structure
analyzed
using
wheat
pan-genome
consisting
20
released
cultivars
(19
are
from
China),
3
single
nucleotide
polymorphisms
(SNPs),
which
were
identified
at
496
bp,
524
1548
bp
CDS
region,
respectively.
Haplotypes
analysis
showed
that
these
SNPs
complete
linkage
disequilibrium
only
two
haplotypes
designated
as
hap1
(TGG)
hap2
(GAA)
present.
Association
between
PHS
four
experiment
environments
revealed
average
accessions
with
significantly
better
than
hap2,
infers
effects
resistance.
To
further
investigate
impacts
alleles
locus
resistance,
SNP
region
converted
KASP
marker,
used
for
genotyping
304
Chinese
cultivars,
whose
evaluated
three
environments.
rates
(SRs)
135
lower
169
validating
present
study
provided
breeding-friendly
marker
functional
variants
can
be
genetic
improvement
