A linkage map of Aegilops biuncialis reveals significant genomic rearrangements compared to bread wheat DOI Creative Commons
Adam Lampar, András Farkas, László Ivanizs

et al.

The Plant Genome, Journal Year: 2025, Volume and Issue: 18(1)

Published: Feb. 26, 2025

Abstract Goatgrasses with U‐ and M‐genomes are important sources of new alleles for wheat breeding to maintain yield quality under extreme conditions. However, the introgression beneficial traits from wild Aegilops species into has been limited by poor knowledge their genomes scarcity molecular tools. Here, we present first linkage map allotetraploid biuncialis Vis., developed using 224 F 2 individuals derived a cross between MvGB382 MvGB642 accessions. The comprises 5663 DArTseq markers assigned 15 groups corresponding 13 chromosomes. Chromosome 1M b could not be constructed due lack recombination caused rearrangements in accession. genetic spans 2518 cM an average marker density 2.79 cM. skeleton contains 920 segregating markers, divided M sub‐genome (425 markers) U (495 markers). Chromosomes sub‐genome, originating comosa Sm. Sibth. et Sm., show well‐preserved collinearity Triticum aestivum L. In contrast, chromosomes umbellulata Zhuk., exhibit varying degree collinearity, 1U , 3U 5U retaining substantial level while 2U 4U 6U 7U significant rearrangements. A quantitative trait locus affecting fertility was identified near centromere on long arm chromosome 3M explaining 23.5% variance. genome structure highlighted map, provides insights speciation within will support alien gene transfer wheat.

Language: Английский

Evolution of wheat blast resistance gene Rmg8 accompanied by differentiation of variants recognizing the powdery mildew fungus DOI
Soichiro Asuke,

Kohei Morita,

Motoki Shimizu

et al.

Nature Plants, Journal Year: 2024, Volume and Issue: 10(6), P. 971 - 983

Published: June 19, 2024

Language: Английский

Citations

10
Heat stress during male meiosis impairs cytoskeletal organization, spindle assembly and tapetum degeneration in wheat DOI Creative Commons
Attila Fábián,

Barbara Krárné Péntek,

Vilmos Soós

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 8, 2024

The significance of heat stress in agriculture is ever-increasing with the progress global climate changes. Due to a negative effect on yield staple crops, including wheat, impairment plant reproductive development triggered by high ambient temperature became restraint food production. Although sensitivity male meiosis and following gamete wheat has long been recognized, detailed structural characterization combined comprehensive gene expression analysis not done about this phenomenon. We demonstrate here that severely alters cytoskeletal configuration, triggers failure meiotic division wheat. Moreover, it changes genes related meiocytes tapetum layer genotype-dependent manner. ‘Ellvis’, heat-tolerant winter cultivar, showed spikelet fertility rate only scarce aberrations upon exposure temperature. In addition, shock involved scavenging reactive oxygen species were significantly upregulated meiosis-specific major developmental stability cultivar. heat-sensitive ‘Mv 17-09’, however, participating fiber nucleation, spindle assembly checkpoint genes, tapetum-specific regulators downregulated. These alterations may be decreased cytoskeleton content, frequent micronuclei formation, erroneous persistence observed sensitive genotype. Our results suggest understanding regulation these functions would an essential contribution new, cultivars.

Language: Английский

Citations

9
Identification of leaf rust resistance loci in hard winter wheat using genome‐wide association mapping DOI Creative Commons

Indira Priyadarshini Lakkakula,

J. A. Kolmer, R. Sharma

et al.

The Plant Genome, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 5, 2025

Abstract Leaf rust, caused by Puccinia triticina ( Pt ), is a serious constraint to wheat production. Developing resistant varieties the best approach managing this disease. Wheat leaf rust resistance Lr ) genes have been classified into either all‐stage (ASR) or adult‐plant (APR). The objectives of study were identify sources in contemporary US hard winter (HWW) and dissect genetic basis underlying HWW. A panel 732 elite HWW genotypes was evaluated for response races at seedling stage adult plant nurseries Oklahoma, Texas, Kansas. Further, genotyped using multiplex restriction amplicon sequencing (MRA‐Seq) DNA markers linked known ASR Lr18 , Lr19 Lr21 Lr24 Lr37 Lr42 APR Lr34 Lr46 Lr67 Lr68 Lr77 Lr78 . Single nucleotide polymorphism (SNP) derived from MRA‐Seq, genes, phenotypic data used genome‐wide association (GWAS) associated with response. Gene postulation based on reactions, markers, GWAS suggested presence Lr1 Lr2a Lr10 Lr14a Lr16 Lr26 Lr39 panel. identified 59 SNPs significantly response, which 20 likely novel loci can be enhance resistance.

Language: Английский

Citations

1
Development of Genome-Wide Unique Indel Markers for a Heat-Sensitive Genotype in Wheat (Triticum aestivum L.) DOI Creative Commons

Huijie Zhai,

Ke Xu, Meng Wang

et al.

Agronomy, Journal Year: 2025, Volume and Issue: 15(1), P. 169 - 169

Published: Jan. 11, 2025

A chromosome segment substituted line (CSSL) represents an ideal resource for studying quantitative traits like thermotolerance. To develop wheat inter-varietal CSSLs with E6015-3S (a heat-sensitive genotype) being the recipient parent, genome-wide unique DNA markers are urgently needed marker-assisted selection. In this study, 11,016 primer pairs targeting 5036 indel sites were successfully designed E6015-3S, average density of 0.36 indels per Mbp. These believed to be and polymorphic in genome; as gathered from evidence, (i) 76.18 99.34% yielded a single hit during sequence alignment 18 sequenced genomes, (ii) 83.59 90.98% 1042 synthesized amplified band 16 accessions, (iii) 59.69 99.81% tested between 15 individual accessions. also anticipated excellent resolvability on agarose or polyacrylamide gels, since most them have sizes 46 bp, amplicon 141 250 polymorphism ratios 6.0 25.0%. Collectively, these CSSL development more broad applications, germplasm classification, seed purity testing, genetic linkage mapping, breeding wheat, owing their uniqueness, polymorphism, easy-to-use characteristics.

Language: Английский

Citations

1
A linkage map of Aegilops biuncialis reveals significant genomic rearrangements compared to bread wheat DOI Creative Commons
Adam Lampar, András Farkas, László Ivanizs

et al.

The Plant Genome, Journal Year: 2025, Volume and Issue: 18(1)

Published: Feb. 26, 2025

Abstract Goatgrasses with U‐ and M‐genomes are important sources of new alleles for wheat breeding to maintain yield quality under extreme conditions. However, the introgression beneficial traits from wild Aegilops species into has been limited by poor knowledge their genomes scarcity molecular tools. Here, we present first linkage map allotetraploid biuncialis Vis., developed using 224 F 2 individuals derived a cross between MvGB382 MvGB642 accessions. The comprises 5663 DArTseq markers assigned 15 groups corresponding 13 chromosomes. Chromosome 1M b could not be constructed due lack recombination caused rearrangements in accession. genetic spans 2518 cM an average marker density 2.79 cM. skeleton contains 920 segregating markers, divided M sub‐genome (425 markers) U (495 markers). Chromosomes sub‐genome, originating comosa Sm. Sibth. et Sm., show well‐preserved collinearity Triticum aestivum L. In contrast, chromosomes umbellulata Zhuk., exhibit varying degree collinearity, 1U , 3U 5U retaining substantial level while 2U 4U 6U 7U significant rearrangements. A quantitative trait locus affecting fertility was identified near centromere on long arm chromosome 3M explaining 23.5% variance. genome structure highlighted map, provides insights speciation within will support alien gene transfer wheat.

Language: Английский

Citations

1