MiniXL: An open-source, large field-of-view epifluorescence miniature microscope for mice capable of single-cell resolution and multi-brain region imaging DOI Creative Commons

Pingping Zhao,

Changliang Guo,

Mian Xie

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 19, 2024

Abstract Capturing the intricate dynamics of neural activity in freely behaving animals is essential for understanding mechanisms underpinning specific behaviors. Miniaturized microscopy enables investigators to track population at cellular level, but field view (FOV) these microscopes have been limited and does not allow multiple-brain region imaging. To fill this technological gap, we developed eXtra Large field-of-view Miniscope (MiniXL), a 3.5g lightweight miniaturized microscope with an FOV measuring 3.5 mm diameter electrically adjustable working distance 1.9 ± 200 μm. We demonstrated capability MiniXL recording large neuronal both subcortical area (hippocampal dorsal CA1) deep brain regions (medial prefrontal cortex, mPFC nucleus accumbens, NAc). The allows simultaneous imaging multiple such as bilateral mPFCs or NAc during complex social behavior tracking cells across sessions. As all UCLA ecosystem, fully open-source will be shared neuroscience community lower barriers adoption technology.

Язык: Английский

Large-scale two-photon calcium imaging in freely moving mice DOI Creative Commons
Weijian Zong, Horst A. Obenhaus,

Emilie Ranheim Skytøen

и другие.

Cell, Год журнала: 2022, Номер 185(7), С. 1240 - 1256.e30

Опубликована: Март 1, 2022

We developed a miniaturized two-photon microscope (MINI2P) for fast, high-resolution, multiplane calcium imaging of over 1,000 neurons at time in freely moving mice. With weight below 3 g and highly flexible connection cable, MINI2P allowed stable with no impediment behavior variety assays compared to untethered, unimplanted animals. The improved cell yield was achieved through optical system design featuring an enlarged field view (FOV) microtunable lens increased z-scanning range speed that allows fast multiple interleaved planes, as well 3D functional imaging. Successive across multiple, adjacent FOVs enabled recordings from more than 10,000 the same animal. Large-scale proof-of-principle data were obtained populations visual cortex, medial entorhinal hippocampus, revealing spatial tuning cells all areas.

Язык: Английский

Процитировано

228
High-resolution Fourier light-field microscopy for volumetric multi-color live-cell imaging DOI Creative Commons
Xuanwen Hua, Wenhao Liu, Shu Jia

и другие.

Optica, Год журнала: 2021, Номер 8(5), С. 614 - 614

Опубликована: Март 18, 2021

Volumetric interrogation of the organization and processes intracellular organelles molecules in cellular systems with a high spatiotemporal resolution is essential for understanding cell physiology, development, pathology. Here, we report high-resolution Fourier light-field microscopy (HR-FLFM) fast volumetric live-cell imaging. HR-FLFM transforms conventional enables exploration less accessible spatiotemporal-limiting regimes single-cell studies. The results present near-diffraction-limited all three dimensions, five-fold extended focal depth to several micrometers, scanning-free volume acquisition time up milliseconds. system demonstrates instrumentation accessibility, low photo damage continuous observation, compatibility general assays. We anticipate offer promising methodological pathway investigating wide range functions exquisite contextual details.

Язык: Английский

Процитировано

59
Multiregion neuronal activity: the forest and the trees DOI
Timothy A. Machado, Isaac Kauvar, Karl Deisseroth

и другие.

Nature reviews. Neuroscience, Год журнала: 2022, Номер 23(11), С. 683 - 704

Опубликована: Окт. 3, 2022

Язык: Английский

Процитировано

42
An optical design enabling lightweight and large field-of-view head-mounted microscopes DOI
Joseph R. Scherrer, Galen F. Lynch, Jie Zhang

и другие.

Nature Methods, Год журнала: 2023, Номер 20(4), С. 546 - 549

Опубликована: Март 16, 2023

Язык: Английский

Процитировано

25
Mesoscale volumetric light-field (MesoLF) imaging of neuroactivity across cortical areas at 18 Hz DOI
Tobias Nöbauer, Yuanlong Zhang, Hyewon Kim

и другие.

Nature Methods, Год журнала: 2023, Номер 20(4), С. 600 - 609

Опубликована: Фев. 23, 2023

Язык: Английский

Процитировано

24
Random-access wide-field mesoscopy for centimetre-scale imaging of biodynamics with subcellular resolution DOI Creative Commons
Ruheng Shi, Xinyue Chen, Junhao Deng

и другие.

Nature Photonics, Год журнала: 2024, Номер 18(7), С. 721 - 730

Опубликована: Апрель 17, 2024

Abstract Benefitting from the advantages of high imaging throughput and low cost, wide-field microscopy has become indispensable in biomedical studies. However, it remains challenging to record biodynamics with a large field view spatiotemporal resolution due limited space–bandwidth product. Here we propose random-access (RA-WiFi) mesoscopy for vivo over 163.84 mm 2 area spatial ~2.18 μm. We extend beyond nominal value objective by enlarging object distance, which leads lower angle, followed correction optical aberrations. also implement scanning structured illumination, enables optical-sectioning capability contrast. The multi-plane makes technique suitable curved-surface samples. demonstrate RA-WiFi multi-modal imaging, including bright-field, dark-field multi-colour fluorescence imaging. Specifically, apply calcium cortex-wide neural network activities awake mice vivo, under both physiological pathological conditions. show its unique three-dimensional random access irregular regions interest via biodynamic mouse spinal cords vivo. As compact, low-cost mesoscope capability, will enable broad applications study biological systems.

Язык: Английский

Процитировано

11
EventLFM: event camera integrated Fourier light field microscopy for ultrafast 3D imaging DOI Creative Commons
Ruipeng Guo,

Qianwan Yang,

Andrew S. Chang

и другие.

Light Science & Applications, Год журнала: 2024, Номер 13(1)

Опубликована: Июнь 26, 2024

Abstract Ultrafast 3D imaging is indispensable for visualizing complex and dynamic biological processes. Conventional scanning-based techniques necessitate an inherent trade-off between acquisition speed space-bandwidth product (SBP). Emerging single-shot wide-field offer a promising alternative but are bottlenecked by the synchronous readout constraints of conventional CMOS systems, thus restricting data throughput to maintain high SBP at limited frame rates. To address this, we introduce EventLFM, straightforward cost-effective system that overcomes these challenges integrating event camera with Fourier light field microscopy (LFM), state-of-the-art technique. The operates on novel asynchronous architecture, thereby bypassing rate limitations systems. We further develop simple robust event-driven LFM reconstruction algorithm can reliably reconstruct dynamics from unique spatiotemporal measurements captured EventLFM. Experimental results demonstrate EventLFM robustly fast-moving rapidly blinking fluorescent samples kHz Furthermore, highlight EventLFM’s capability neuronal signals in scattering mouse brain tissues tracking GFP-labeled neurons freely moving C. elegans . believe combined ultrafast large offered may open up new possibilities across many biomedical applications.

Язык: Английский

Процитировано

11
Long-term mesoscale imaging of 3D intercellular dynamics across a mammalian organ DOI Creative Commons
Yuanlong Zhang, Mingrui Wang,

Qiyu Zhu

и другие.

Cell, Год журнала: 2024, Номер unknown

Опубликована: Сен. 1, 2024

Язык: Английский

Процитировано

11
Deep-learning-augmented computational miniature mesoscope DOI Creative Commons
Yujia Xue,

Qianwan Yang,

Guorong Hu

и другие.

Optica, Год журнала: 2022, Номер 9(9), С. 1009 - 1009

Опубликована: Авг. 3, 2022

Fluorescence microscopy is essential to study biological structures and dynamics. However, existing systems suffer from a tradeoff between field-of-view (FOV), resolution, complexity, thus cannot fulfill the emerging need of miniaturized platforms providing micron-scale resolution across centimeter-scale FOVs. To overcome this challenge, we developed Computational Miniature Mesoscope (CM$^2$) that exploits computational imaging strategy enable single-shot 3D high-resolution wide FOV in platform. Here, present CM$^2$ V2 significantly advances both hardware computation. We complement 3$\times$3 microlens array with new hybrid emission filter improves contrast by 5$\times$, design 3D-printed freeform collimator for LED illuminator excitation efficiency 3$\times$. reconstruction large volume, develop an accurate efficient linear shift-variant (LSV) model characterizes spatially varying aberrations. then train multi-module deep learning model, CM$^2$Net, using only 3D-LSV simulator. show CM$^2$Net generalizes well experiments achieves $\sim$7-mm 800-$\mu$m depth, provides $\sim$6-$\mu$m lateral $\sim$25-$\mu$m axial resolution. This $\sim$8$\times$ better localization $\sim$1400$\times$ faster speed as compared previous model-based algorithm. anticipate simple low-cost miniature system will be impactful many large-scale fluorescence applications.

Язык: Английский

Процитировано

39
Neurophotonic Tools for Microscopic Measurements and Manipulation: Status Report DOI Creative Commons
Ahmed S. Abdelfattah,

Srinivasa Rao Allu,

Robert E. Campbell

и другие.

Neurophotonics, Год журнала: 2022, Номер 9(S1)

Опубликована: Апрель 27, 2022

Neurophotonics was launched in 2014 coinciding with the launch of BRAIN Initiative focused on development technologies for advancement neuroscience. For last seven years, Neurophotonics' agenda has been well aligned this focus neurotechnologies featuring new optical methods and tools applicable to brain studies. While 2.0 is pivoting towards applications these novel quest understand brain, article we review an extensive diverse toolkit explore function that have emerged from related large-scale efforts measurement manipulation structure function. Here, neurophotonic mostly animal A companion article, scheduled appear later year, will cover diffuse imaging noninvasive human each domain, outline current state-of-the-art respective technologies, identify areas where innovation needed provide outlook future directions.

Язык: Английский

Процитировано

33