Cellular and Molecular Life Sciences,
Год журнала:
2023,
Номер
80(8)
Опубликована: Июль 28, 2023
Abstract
Microenvironmental
factors
are
known
fundamental
regulators
of
the
phenotype
and
aggressiveness
glioblastoma
(GBM),
most
lethal
brain
tumor,
characterized
by
fast
progression
marked
resistance
to
treatments.
In
this
context,
extracellular
matrix
(ECM)
is
heavily
influence
behavior
cancer
cells
from
several
origins,
contributing
stem
cell
niches,
influencing
tumor
invasiveness
response
chemotherapy,
mediating
survival
signaling
cascades,
modulating
inflammatory
recruitment.
Here,
we
show
that
collagen
VI
(COL6),
an
ECM
protein
widely
expressed
in
both
normal
pathological
tissues,
has
a
distinctive
distribution
within
GBM
mass,
strongly
correlated
with
aggressive
phenotypically
immature
cells.
Our
data
demonstrate
COL6
sustains
stem-like
properties
supports
maintenance
transcriptional
program
promoting
proliferation
survival.
particular,
identified
specific
subset
COL6-transcriptionally
co-regulated
genes,
required
for
replicative
stress
DNA
damage,
supporting
concept
essential
stimulus
activation
through
ATM/ATR
axis.
Altogether,
these
findings
indicate
plays
pivotal
role
biology,
exerting
pleiotropic
action
across
different
hallmarks,
including
phenotypic
identity
gene
transcription,
as
well
treatments,
thus
providing
valuable
information
understanding
complex
microenvironmental
cues
underlying
malignancy.
Biomaterials Science,
Год журнала:
2022,
Номер
11(2), С. 400 - 431
Опубликована: Дек. 2, 2022
Decellularized
extracellular
matrix
hydrogels
are
tissue-derived
materials
that
with
proper
processing
can
be
used
for
tissue
engineering
applications
and
to
build
microenvironments
in
vitro
.
Figure
was
partly
created
Servier
Medical
Art.
Cancer Research,
Год журнала:
2022,
Номер
82(10), С. 2031 - 2044
Опубликована: Март 8, 2022
Abstract
Triple-negative
breast
cancer
(TNBC)
is
the
most
aggressive
and
deadly
subtype
of
cancer,
accounting
for
30,000
cases
annually
in
United
States.
While
there
are
several
clinical
trials
ongoing
to
identify
new
agents
treat
TNBC,
majority
patients
with
TNBC
treated
anthracycline-
or
taxane-based
chemotherapies
neoadjuvant
setting,
followed
by
surgical
resection
adjuvant
chemotherapy.
many
respond
well
this
approach,
as
25%
will
suffer
local
metastatic
recurrence
within
5
years.
Understanding
mechanisms
that
drive
after
chemotherapy
treatment
critical
improving
survival
TNBC.
It
established
extracellular
matrix
(ECM),
which
provides
structure
support
tissues,
a
major
driver
tumor
growth,
invasion,
dissemination
cells
distant
sites.
In
present
study,
we
show
decellularized
ECM
(dECM)
obtained
from
chemotherapy-treated
mice
increases
motility
treatment-naïve
compared
vehicle-treated
dECM.
Tandem-mass–tag
proteomics
revealed
induce
drug-specific
changes
composition.
The
basement
membrane
protein
collagen
IV
was
significantly
upregulated
Collagen
drove
invasion
via
activation
Src
focal
adhesion
kinase
signaling
downstream
integrin
α1
α2,
inhibition
IV–driven
decreased
These
studies
provide
novel
mechanism
may
metastasis
its
effects
on
Significance:
Cytotoxic
induces
significant
composition
ECM,
inducing
more
invasive
phenotype
residual
following
Biomedicine & Pharmacotherapy,
Год журнала:
2023,
Номер
166, С. 115390 - 115390
Опубликована: Сен. 4, 2023
The
tumor
microenvironment
(TME)
is
crucial
in
cancer
progression,
and
the
extracellular
matrix
(ECM)
an
important
TME
component.
Collagen
a
major
ECM
component
that
contributes
to
cell
infiltration,
expansion,
distant
metastasis
during
progression.
Recent
studies
reported
collagen
deposited
form
wall
along
which
cells
can
infiltrate
prevent
drugs
from
working
on
cells.
Collagen-tumor
interaction
complex
requires
activation
of
multiple
signaling
pathways
for
biochemical
mechanical
interventions.
In
this
review,
we
examine
effect
deposition
progression
discuss
between
This
review
aims
illustrate
functions
mechanisms
its
role
therapy.
findings
indicated
appears
be
better
target
British Journal of Cancer,
Год журнала:
2023,
Номер
129(12), С. 1877 - 1892
Опубликована: Окт. 4, 2023
Abstract
Thioredoxin-interacting
protein
(TXNIP)
is
commonly
considered
a
master
regulator
of
cellular
oxidation,
regulating
the
expression
and
function
Thioredoxin
(Trx).
Recent
work
has
identified
that
TXNIP
far
wider
range
additional
roles:
from
glucose
lipid
metabolism,
to
cell
cycle
arrest
inflammation.
Its
increased
by
stressors
found
in
neoplastic
cells
tumor
microenvironment
(TME),
and,
as
such,
been
extensively
studied
cancers.
In
this
review,
we
evaluate
current
literature
regarding
regulation
TXNIP,
highlighting
its
emerging
role
modulating
signaling
between
different
types
within
TME.
We
then
assess
future
translational
opportunities
associated
challenges
area.
An
improved
understanding
functions
mechanisms
cancers
may
enhance
suitability
therapeutic
target.
Breast Cancer Research,
Год журнала:
2024,
Номер
26(1)
Опубликована: Март 11, 2024
Abstract
Background
Metastasis
is
the
leading
cause
of
death
in
breast
cancer
patients.
For
metastasis
to
occur,
tumor
cells
must
invade
locally,
intravasate,
and
colonize
distant
tissues
organs,
all
steps
that
require
cell
migration.
The
majority
studies
on
invasion
rely
human
lines.
While
it
known
these
have
different
properties
abilities
for
growth
metastasis,
vitro
morphological,
proliferative,
migratory,
invasive
behavior
lines
their
correlation
vivo
poorly
understood.
Thus,
we
sought
classify
each
line
as
or
highly
metastatic
by
characterizing
a
murine
model
six
commonly
used
triple-negative
xenografts,
well
determine
which
assays
study
motility
best
predict
metastasis.
Methods
We
evaluated
liver
lung
TNBC
MDA-MB-231,
MDA-MB-468,
BT549,
Hs578T,
BT20,
SUM159
immunocompromised
mice.
characterized
line's
morphology,
proliferation,
2D
3D
variation
parameters
between
Results
identified
BT549
tumorigenic
metastatic,
Hs578T
BT20
intermediate
with
poor
lungs
but
livers,
livers.
showed
metrics
characterize
morphology
are
most
predictive
potential
liver.
Further,
found
no
single
assay
significantly
correlated
.
Conclusions
Our
results
provide
an
important
resource
research
community,
identifying
6
findings
also
support
use
morphological
analysis
investigate
emphasize
need
multiple
using
represent
heterogeneity
vivo.
Materials Today Bio,
Год журнала:
2024,
Номер
25, С. 101004 - 101004
Опубликована: Фев. 16, 2024
Extracellular
matrix
(ECM)
stiffening
is
a
common
occurrence
during
the
progression
of
many
diseases,
such
as
breast
cancer.
To
accurately
mimic
pathophysiological
context
disease
within
3D
in
vitro
models,
there
high
demand
for
smart
biomaterials
which
replicate
dynamic
and
temporal
mechanical
cues
diseased
states.
This
study
describes
preclinical
model,
using
cancer
an
example,
replicates
plasticity
tumour
microenvironment
by
incorporating
(3-week
progression)
biomechanical
tissue-specific
hydrogel
microenvironment.
The
composite
formulation,
integrating
adipose-derived
decellularised
ECM
(AdECM)
silk
fibroin,
was
initially
crosslinked
visible
light-mediated
system,
then
progressively
stiffened
through
spontaneous
secondary
structure
interactions
inherent
between
polymer
chains
(∼10–15
kPa
increase,
with
final
stiffness
25
kPa).
When
encapsulated
cultured
vitro,
MCF-7
cells
formed
numerous,
large
spheroids
(>1000
μm2
area),
however,
progressive
stiffening,
demonstrated
growth
arrest
underwent
phenotypic
changes
resulting
intratumoral
heterogeneity.
Unlike
widely-investigated
static
this
allowed
to
be
observed,
fostered
development
mature
organoid-like
spheroids,
mimicked
both
organisation
acinar-structures
epithelium.
contained
central
population
expressed
aggressive
cellular
programs,
evidenced
increased
fibronectin
expression
reduction
E-cadherin.
heterogeneity
observed
model
more
reflective
physiological
tumours,
demonstrating
importance
establishing
models
future
work.
Overall,
developed
novel
strategy
uncouple
properties
from
complexities
offers
potential
wide
applicability
other
addition
dECM
materials.
Journal of Cell Communication and Signaling,
Год журнала:
2024,
Номер
18(2)
Опубликована: Июнь 1, 2024
Abstract
Obesity,
a
rapidly
expanding
epidemic
worldwide,
is
known
to
exacerbate
many
medical
conditions,
making
it
significant
factor
in
multiple
diseases
and
their
associated
complications.
This
threatening
linked
various
harmful
conditions
such
as
type
2
diabetes
mellitus,
hypertension,
metabolic
dysfunction‐associated
steatotic
liver
disease,
polycystic
ovary
syndrome,
cardiovascular
(CVDs),
dyslipidemia,
cancer.
The
rise
urbanization
sedentary
lifestyles
creates
an
environment
that
fosters
obesity,
leading
both
psychosocial
To
identify
individuals
at
risk
ensure
timely
treatment,
crucial
have
better
understanding
of
the
pathophysiology
obesity
its
comorbidities.
comprehensive
review
highlights
relationship
between
obesity‐associated
complications,
including
diabetes,
gastrointestinal
obstructive
sleep
apnea.
It
also
explores
potential
mechanisms
underlying
these
associations.
A
thorough
analysis
interplay
complications
vital
developing
effective
therapeutic
strategies
combat
exponential
increase
global
rates
mitigate
deadly
consequences
this
polygenic
condition.
Colorectal
cancer
(CRC)
is
the
third
most
common
worldwide
and
has
one
of
highest
mortality
rates.
Considering
its
nonlinear
etiology,
many
risk
factors
are
associated
with
CRC
formation
development,
obesity
at
forefront.
Obesity
regarded
as
key
environmental
determinants
for
pathogenesis
CRC.
Excessive
food
intake
a
sedentary
lifestyle,
together
genetic
predispositions,
lead
to
overgrowth
adipose
tissue
along
disruption
in
number
function
building
cells.
Adipose
tissue-resident
cells
may
constitute
part
microenvironment.
Alterations
their
physiology
secretory
profiles
observed
further
contribute
progression,
despite
similar
localization,
contributions
not
equivalent.
They
can
interact
cells,
either
directly
or
indirectly,
influencing
various
processes
that
tumorigenesis.
The
main
aim
this
review
provide
insights
into
diversity
resident
namely,
adipocytes,
stromal
immunological
regarding
role
particular
cell
types
co-forming
scope
study
was
also
devoted
abnormalities
states
impact
on
development.
