ACS ES&T Engineering,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 15, 2024
Phototrophs
with
heterotrophic
bacterial
consortium
as
an
electrode
biocatalyst
are
emerging
concept
for
developing
naturally
sustained
biophotovoltaic
systems.
Herein,
Spirulina
subsalsa-based
mixed
community
anodic
catalyst
in
a
microbial
fuel
cell
(MFC)
setup
ferricyanide
catholyte
78
days
light–dark
(16–8
h)
cycle-based
operation
was
investigated.
The
biofilm
developed
inducted
recalcitrant
comprising
Halomonas,
Alcanivorax,
Pelagibacterium,
and
Rhizobiales
the
major
genera.
In
extended
dark
phase
(9
days)
within
cyclic
operation,
sequential
shift
of
metabolism
from
photosynthesis
to
fermentative
states
increased
population
were
observed.
Under
direct
contact
graphite
anode,
initiated
oscillating
open-circuit
potentials
MFC
response
circadian
trend.
delivered
maxima
587
μW
m–2
418
(at
10
kΩ)
under
corresponding
phases,
respectively.
potential
shifted
more
negative
value,
reaching
−415.5
mV
starvation
period.
Analyses
reaction
rates
(extracted
Tafel
plots),
corrosion
potential,
current,
polarization
resistance,
residual
redox
charges
voltammograms)
performed
understand
processes.
Two
peaks
0.6
V
(irreversible,
extracellular)
0.26
(reversible,
cell-surface
attached)
attributed
mediation
this
process.
Additionally,
catholyte-diffused
interacts
biofilm,
getting
trapped
matrix
polymeric
structures,
thus
preventing
sudden
cytotoxic
elimination
cells
promoting
oxidative
charge
accumulation
over
its
surface,
improving
potential.
Rapid
respiratory
oxygen
consumption,
biofilm's
structural
reorganization,
ferricyanide's
chemical
speciation
inside
primary
factors
that
govern
performance
biofuel
during
prolonged
operations.
critical
findings
unveiled
through
study
advance
our
understanding
resilience
phototroph-based
multispecies
catalysts
devices
long-term
Abstract
Background
Phosphonates
are
the
main
components
in
global
phosphorus
redox
cycle.
Little
is
known
about
phosphonate
metabolism
freshwater
ecosystems,
although
rapid
consumption
of
phosphonates
has
been
observed
frequently.
Cyanobacteria
often
dominant
primary
producers
freshwaters;
yet,
only
a
few
strains
cyanobacteria
encode
phosphonate-degrading
(C-P
lyase)
gene
clusters.
The
phycosphere
defined
as
microenvironment
which
extensive
phytoplankton
and
heterotrophic
bacteria
interactions
occur.
It
demonstrated
that
may
recruit
phycospheric
based
on
their
own
needs.
Therefore,
establishment
community
rich
phosphonate-degrading-bacteria
likely
facilitates
cyanobacterial
proliferation,
especially
waters
with
scarce
phosphorus.
We
characterized
distribution
field
Microcystis
bloom
samples
laboratory
“phycospheres”
by
qPCR
metagenomic
analyses.
role
proliferation
was
determined
through
coculturing
an
axenic
aeruginosa
strain
metatranscriptomic
analysis
using
aggregate
.
Results
Abundant
carry
C-P
lyase
clusters
were
identified
plankton
from
Lakes
Dianchi
Taihu
during
periods.
Metagenomic
162
non-axenic
(consortia
cultures
containing
bacteria)
showed
20%
(128/647)
high-quality
bins
eighty
these
consortia
intact
clusters,
abundance
ranging
up
to
nearly
13%.
Phycospheric
bacterial
catabolism
genes
expressed
continually
across
seasons,
sixteen
samples.
Coculturing
experiments
revealed
did
not
catabolize
methylphosphonate
when
axenic,
they
sustained
growth
cocultured
phosphonate-utilizing
medium
sole
source
Conclusions
recruitment
hedge
against
scarcity
facilitating
availability.
Cyanobacterial
contributors
aquatic
mineralization,
thereby
growth,
even
maintenance,
phosphate-deficient
waters.
Environmental Science & Technology,
Год журнала:
2024,
Номер
58(13), С. 5796 - 5810
Опубликована: Март 20, 2024
Globally
kelp
farming
is
gaining
attention
to
mitigate
land-use
pressures
and
achieve
carbon
neutrality.
However,
the
influence
of
environmental
perturbations
on
remains
largely
unknown.
Recently,
a
severe
disease
outbreak
caused
extensive
mortality
in
Sanggou
Bay,
China,
one
world's
largest
high-density
areas.
Here,
through
situ
investigations
simulation
experiments,
we
find
indications
that
an
anomalously
dramatic
increase
elevated
coastal
seawater
light
penetration
may
have
contributed
dysbiosis
Saccharina
japonica's
microbiome.
This
promoted
proliferation
opportunistic
pathogenic
Enterobacterales,
mainly
including
genera
Colwellia
Pseudoalteromonas.
Using
transcriptomic
analyses,
revealed
high-light
conditions
likely
induced
oxidative
stress
kelp,
potentially
facilitating
bacterial
Enterobacterales
attack
activates
terrestrial
plant-like
pattern
recognition
receptor
system
kelp.
Furthermore,
uncover
crucial
genotypic
determinants
dominance
pathogenicity
within
tissue,
pathogen-associated
molecular
patterns,
potential
membrane-damaging
toxins,
alginate
mannitol
lysis
capability.
Finally,
analysis
kelp-associated
microbiome
data
sets
under
ocean
warming
acidification,
conclude
such
favoring
shifts
are
become
more
prevalent
future
conditions.
Our
study
highlights
need
for
understanding
complex
influences
health
associated
microbiomes
sustainable
development
seaweed
farming.
The ISME Journal,
Год журнала:
2023,
Номер
17(11), С. 1979 - 1992
Опубликована: Сен. 8, 2023
Abstract
Algae
and
bacteria
have
complex
intimate
interactions
in
the
ocean.
Besides
mutualism,
evolved
a
variety
of
molecular-based
anti-algal
strategies.
However,
limited
by
unknown
mechanism
synthesis
action
these
molecules,
strategies
their
global
prevalence
remain
unknown.
Here
we
identify
novel
strategy
through
which
marine
representative
Gammaproteobacteria
produced
3,3’,5,5’-tetrabromo-2,2’-biphenyldiol
(4-BP),
that
kills
or
inhibits
diverse
phytoplankton
inhibiting
plastoquinone
its
effect
cascades
to
many
other
key
metabolic
processes
algae.
Through
comparative
genomic
analysis
between
4-BP-producing
bacterium
algicidally
inactive
mutant,
combined
with
gene
function
verification,
identified
cluster
responsible
for
4-BP
synthesis,
contains
genes
encoding
chorismate
lyase,
flavin-dependent
halogenase
cytochrome
P450.
We
demonstrated
near
situ
simulated
algal
blooming
seawater,
even
low
concentrations
can
cause
changes
overall
community
structure
decline
dinoflagellates
diatoms.
Further
analyses
sequences
from
Tara
Oceans
expeditions
2750
whole
genome
confirmed
ubiquitous
presence
synthetic
bacterial
members
ocean,
suggesting
it
is
tool
potentially
widely
used
oceans
mediate
bacteria-algae
antagonistic
relationships.
Environment International,
Год журнала:
2024,
Номер
188, С. 108753 - 108753
Опубликована: Май 15, 2024
Fermentation
broth
from
fruit
and
vegetable
waste
(FFVW)
has
demonstrated
remarkable
ability
as
a
soil
amendment
in
reducing
antibiotic
resistance
genes
(ARGs)
pollution.
However,
the
potential
of
FFVW
to
mitigate
other
microbial
contamination
such
human
bacterial
pathogens
(HBPs)
virulence
factor
(VFGs),
which
are
closely
associated
with
health,
remains
unknown.
In
this
study,
metagenomic
analysis
revealed
that
reduced
HBPs
high-risk
ARGs
VFGs
including
Klebsiella
pneumoniae
(reduced
by
40.4
%),
Mycobacterium
tuberculosis
21.4
%)
Streptococcus
38.7
%).
Correspondingly,
VFG
abundance
decreased
3.40
copies/cell
2.99
copies/cell.
Further
illustrated
these
was
mainly
attributed
inhibition
quorum
sensing
(QS).
QS
signals,
synthesis
rpaI
luxS,
well
receptor
rpfC
fusK,
resulting
risk
VFGs.
The
pure
culture
experiment
expression
related
QS,
VFGs,
mobile
genetic
elements
(MGEs)
were
downregulated
Pseudomonas
aeruginosa,
Staphylococcus
aureus,
Escherichia
coli
K.
treated
FFVW,
consistent
result
analysis.
This
study
suggested
an
environmentally
friendly
approach
for
controlling
VFGs/ARGs-carrying
HBPs,
is
crucial
both
health
under
framework
"One
Health".
Picocyanobacteria
from
the
genera
Prochlorococcus,
Synechococcus,
and
Cyanobium
are
most
widespread
photosynthetic
organisms
in
aquatic
ecosystems.
However,
their
freshwater
populations
remain
poorly
explored,
due
to
uneven
insufficient
sampling
across
diverse
inland
waterbodies.
