Spirulina-Based Multispecies Phototrophic Biofilm Anodic Biocatalyst Endures a Prolonged Dark Phase within Light–Dark Cycle Operations and Enhances Anodic Performance in Biophotovoltaic Cells DOI

Mohd Golam Abdul Quadir,

Nabajyoti Kalita,

Pranab Goswami

et al.

ACS ES&T Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 15, 2024

Phototrophs with heterotrophic bacterial consortium as an electrode biocatalyst are emerging concept for developing naturally sustained biophotovoltaic systems. Herein, Spirulina subsalsa-based mixed community anodic catalyst in a microbial fuel cell (MFC) setup ferricyanide catholyte 78 days light–dark (16–8 h) cycle-based operation was investigated. The biofilm developed inducted recalcitrant comprising Halomonas, Alcanivorax, Pelagibacterium, and Rhizobiales the major genera. In extended dark phase (9 days) within cyclic operation, sequential shift of metabolism from photosynthesis to fermentative states increased population were observed. Under direct contact graphite anode, initiated oscillating open-circuit potentials MFC response circadian trend. delivered maxima 587 μW m–2 418 (at 10 kΩ) under corresponding phases, respectively. potential shifted more negative value, reaching −415.5 mV starvation period. Analyses reaction rates (extracted Tafel plots), corrosion potential, current, polarization resistance, residual redox charges voltammograms) performed understand processes. Two peaks 0.6 V (irreversible, extracellular) 0.26 (reversible, cell-surface attached) attributed mediation this process. Additionally, catholyte-diffused interacts biofilm, getting trapped matrix polymeric structures, thus preventing sudden cytotoxic elimination cells promoting oxidative charge accumulation over its surface, improving potential. Rapid respiratory oxygen consumption, biofilm's structural reorganization, ferricyanide's chemical speciation inside primary factors that govern performance biofuel during prolonged operations. critical findings unveiled through study advance our understanding resilience phototroph-based multispecies catalysts devices long-term

Language: Английский

Dissolved organic carbon spurs bacterial-algal competition and phosphorus-paucity adaptation: Boosting Microcystis' phosphorus uptake capacity DOI
Tingting Li, Longqian Xu, Wenxuan Li

et al.

Water Research, Journal Year: 2024, Volume and Issue: 255, P. 121465 - 121465

Published: March 13, 2024

Language: Английский

Citations

10
The facilitating role of phycospheric heterotrophic bacteria in cyanobacterial phosphonate availability and Microcystis bloom maintenance DOI Creative Commons

Liang Zhao,

Lizhou Lin,

Ying Zeng

et al.

Microbiome, Journal Year: 2023, Volume and Issue: 11(1)

Published: June 26, 2023

Abstract Background Phosphonates are the main components in global phosphorus redox cycle. Little is known about phosphonate metabolism freshwater ecosystems, although rapid consumption of phosphonates has been observed frequently. Cyanobacteria often dominant primary producers freshwaters; yet, only a few strains cyanobacteria encode phosphonate-degrading (C-P lyase) gene clusters. The phycosphere defined as microenvironment which extensive phytoplankton and heterotrophic bacteria interactions occur. It demonstrated that may recruit phycospheric based on their own needs. Therefore, establishment community rich phosphonate-degrading-bacteria likely facilitates cyanobacterial proliferation, especially waters with scarce phosphorus. We characterized distribution field Microcystis bloom samples laboratory “phycospheres” by qPCR metagenomic analyses. role proliferation was determined through coculturing an axenic aeruginosa strain metatranscriptomic analysis using aggregate . Results Abundant carry C-P lyase clusters were identified plankton from Lakes Dianchi Taihu during periods. Metagenomic 162 non-axenic (consortia cultures containing bacteria) showed 20% (128/647) high-quality bins eighty these consortia intact clusters, abundance ranging up to nearly 13%. Phycospheric bacterial catabolism genes expressed continually across seasons, sixteen samples. Coculturing experiments revealed did not catabolize methylphosphonate when axenic, they sustained growth cocultured phosphonate-utilizing medium sole source Conclusions recruitment hedge against scarcity facilitating availability. Cyanobacterial contributors aquatic mineralization, thereby growth, even maintenance, phosphate-deficient waters.

Language: Английский

Citations

22
Adverse Environmental Perturbations May Threaten Kelp Farming Sustainability by Exacerbating Enterobacterales Diseases DOI
Yongyu Zhang, Shailesh Nair, Zenghu Zhang

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(13), P. 5796 - 5810

Published: March 20, 2024

Globally kelp farming is gaining attention to mitigate land-use pressures and achieve carbon neutrality. However, the influence of environmental perturbations on remains largely unknown. Recently, a severe disease outbreak caused extensive mortality in Sanggou Bay, China, one world's largest high-density areas. Here, through situ investigations simulation experiments, we find indications that an anomalously dramatic increase elevated coastal seawater light penetration may have contributed dysbiosis Saccharina japonica's microbiome. This promoted proliferation opportunistic pathogenic Enterobacterales, mainly including genera Colwellia Pseudoalteromonas. Using transcriptomic analyses, revealed high-light conditions likely induced oxidative stress kelp, potentially facilitating bacterial Enterobacterales attack activates terrestrial plant-like pattern recognition receptor system kelp. Furthermore, uncover crucial genotypic determinants dominance pathogenicity within tissue, pathogen-associated molecular patterns, potential membrane-damaging toxins, alginate mannitol lysis capability. Finally, analysis kelp-associated microbiome data sets under ocean warming acidification, conclude such favoring shifts are become more prevalent future conditions. Our study highlights need for understanding complex influences health associated microbiomes sustainable development seaweed farming.

Language: Английский

Citations

8
Plastoquinone synthesis inhibition by tetrabromo biphenyldiol as a widespread algicidal mechanism of marine bacteria DOI Creative Commons
Zenghu Zhang, Dehai Li, Ruize Xie

et al.

The ISME Journal, Journal Year: 2023, Volume and Issue: 17(11), P. 1979 - 1992

Published: Sept. 8, 2023

Abstract Algae and bacteria have complex intimate interactions in the ocean. Besides mutualism, evolved a variety of molecular-based anti-algal strategies. However, limited by unknown mechanism synthesis action these molecules, strategies their global prevalence remain unknown. Here we identify novel strategy through which marine representative Gammaproteobacteria produced 3,3’,5,5’-tetrabromo-2,2’-biphenyldiol (4-BP), that kills or inhibits diverse phytoplankton inhibiting plastoquinone its effect cascades to many other key metabolic processes algae. Through comparative genomic analysis between 4-BP-producing bacterium algicidally inactive mutant, combined with gene function verification, identified cluster responsible for 4-BP synthesis, contains genes encoding chorismate lyase, flavin-dependent halogenase cytochrome P450. We demonstrated near situ simulated algal blooming seawater, even low concentrations can cause changes overall community structure decline dinoflagellates diatoms. Further analyses sequences from Tara Oceans expeditions 2750 whole genome confirmed ubiquitous presence synthetic bacterial members ocean, suggesting it is tool potentially widely used oceans mediate bacteria-algae antagonistic relationships.

Language: Английский

Citations

13
The novel application of violacein produced by a marine Duganella strain as a promising agent for controlling Heterosigma akashiwo bloom: Algicidal mechanism, fermentation optimization and agent formulation DOI
Guanjing Cai, Xujun Yang, Xiaoqi Yu

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 466, P. 133548 - 133548

Published: Jan. 18, 2024

Language: Английский

Citations

5
Intensified anthropogenic disturbances impair planktonic algae in an urban river DOI
Jiawen Wang,

Shanqing Yang,

Yucheng Tian

et al.

Journal of Cleaner Production, Journal Year: 2024, Volume and Issue: 468, P. 143091 - 143091

Published: July 6, 2024

Language: Английский

Citations

4
Understanding the roles of viruses as key players in environmental dynamics and ecosystem functioning DOI Creative Commons
M. Abbasi, Masrure Alam

Discover Viruses., Journal Year: 2025, Volume and Issue: 2(1)

Published: April 17, 2025

Language: Английский

Citations

0
Fermentation broth from fruit and vegetable waste works: Reducing the risk of human bacterial pathogens in soil by inhibiting quorum sensing DOI Creative Commons
Lin Zhu, Jingpeng Li, Jian Yang

et al.

Environment International, Journal Year: 2024, Volume and Issue: 188, P. 108753 - 108753

Published: May 15, 2024

Fermentation broth from fruit and vegetable waste (FFVW) has demonstrated remarkable ability as a soil amendment in reducing antibiotic resistance genes (ARGs) pollution. However, the potential of FFVW to mitigate other microbial contamination such human bacterial pathogens (HBPs) virulence factor (VFGs), which are closely associated with health, remains unknown. In this study, metagenomic analysis revealed that reduced HBPs high-risk ARGs VFGs including Klebsiella pneumoniae (reduced by 40.4 %), Mycobacterium tuberculosis 21.4 %) Streptococcus 38.7 %). Correspondingly, VFG abundance decreased 3.40 copies/cell 2.99 copies/cell. Further illustrated these was mainly attributed inhibition quorum sensing (QS). QS signals, synthesis rpaI luxS, well receptor rpfC fusK, resulting risk VFGs. The pure culture experiment expression related QS, VFGs, mobile genetic elements (MGEs) were downregulated Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli K. treated FFVW, consistent result analysis. This study suggested an environmentally friendly approach for controlling VFGs/ARGs-carrying HBPs, is crucial both health under framework "One Health".

Language: Английский

Citations

2
Uncovering the genomic basis of symbiotic interactions and niche adaptations in freshwater picocyanobacteria DOI Creative Commons

Hongjae Park,

Paul‐Adrian Bulzu, Tanja Shabarova

et al.

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: Aug. 10, 2024

Picocyanobacteria from the genera Prochlorococcus, Synechococcus, and Cyanobium are most widespread photosynthetic organisms in aquatic ecosystems. However, their freshwater populations remain poorly explored, due to uneven insufficient sampling across diverse inland waterbodies.

Language: Английский

Citations

2
Light-dark fluctuated metabolic features of diazotrophic and non-diazotrophic cyanobacteria and their coexisting bacteria DOI
Hao Zhang, Yehui Tan, Youping Zhou

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 910, P. 168702 - 168702

Published: Nov. 20, 2023

Language: Английский

Citations

6