Analysis of the correlation between cervical HPV infection, cervical lesions and vaginal microecology DOI Creative Commons
Zhongru Fan,

Dongyu Han,

Xinxiang Fan

и другие.

Frontiers in Cellular and Infection Microbiology, Год журнала: 2024, Номер 14

Опубликована: Авг. 16, 2024

Background Vaginal microbiota is involved in human papillomavirus (HPV) infection and cervical cancer (CC) progression, the specific changes vaginal microbial composition during this process remains uncertain. Objective This study aimed to observe of microorganisms different lesions identify biomarkers at stages lesions. Methods In we used illumina high-throughput gene sequencing technology determine V4 region 16SrRNA observed Results The patients with high-risk HPV significantly from that normal population, but there no significant difference richness microbes. diversity species CC higher than or CIN patients. main manifestation an increase microbes, a decrease relative abundance cyanobacteria Lactobacillus, dialister, peptonephila other miscellaneous bacteria. There are characteristic biomarker women, high risk detail, group was varibaculum, saccharopolyspora, Proteobacteria, Corynebacterium, Coprococcus, Peptococcus Ruminococcus. Conclusions indicated compositions microbes different. has certain diagnostic effect on healthy These may serve as potential for CC. It also provided effective way treatment infections

Язык: Английский

Nanozymes for nanohealthcare DOI
Yihong Zhang, Gen Wei, W. Liu

и другие.

Nature Reviews Methods Primers, Год журнала: 2024, Номер 4(1)

Опубликована: Май 30, 2024

Язык: Английский

Процитировано

81

Designing nanozymes for in vivo applications DOI
Ruofei Zhang, Bing Jiang, Kelong Fan

и другие.

Nature Reviews Bioengineering, Год журнала: 2024, Номер 2(10), С. 849 - 868

Опубликована: Июль 18, 2024

Язык: Английский

Процитировано

79

On the Specificity of Nanozymes: A Perspective DOI
Gen Wei, Shujie Liu, Yung‐Kang Peng

и другие.

Chinese Journal of Chemistry, Год журнала: 2024, Номер 42(13), С. 1515 - 1522

Опубликована: Март 9, 2024

Comprehensive Summary We have compiled eight promising strategies for enhancing the specificity and selectivity of nanozymes, as depicted in comprehensive summary above. Enzymes exhibit intricate sophisticated structures, including substrate channels active sites, which can inform design nanozymes. Replication these structural features application facet engineering/doping techniques significantly enhance catalytic Alternatively, use Molecularly Imprinted Polymers (MIPs) to coat nanozymes represents an effective approach impart specificity. Furthermore, several straightforward stopgap been devised improve nanozyme analytical applications, such integration biorecognition elements sensor arrays through surface modification. image Key Scientists

Язык: Английский

Процитировано

37

Nanozybiotics: Advancing Antimicrobial Strategies Through Biomimetic Mechanisms DOI
Caiyu Zhou, Qian Wang,

Haolin Cao

и другие.

Advanced Materials, Год журнала: 2024, Номер 36(33)

Опубликована: Июнь 14, 2024

Infectious diseases caused by bacterial, viral, and fungal pathogens present significant global health challenges. The rapid emergence of antimicrobial resistance exacerbates this issue, leading to a scenario where effective antibiotics are increasingly scarce. Traditional antibiotic development strategies proving inadequate against the swift evolution microbial resistance. Therefore, there is an urgent need develop novel with mechanisms distinct from those existing antibiotics. Nanozybiotics, which nanozyme-based antimicrobials, mimic catalytic action lysosomal enzymes in innate immune cells kill infectious pathogens. This review reinforces concept nanozymes provides comprehensive summary recent research advancements on potential candidates. Initially, nanozybiotics categorized based their activities, mimicking either oxidoreductase-like or hydrolase-like functions, thereby highlighting superior combating then discusses progress treating infections, confirming as translational nanozybiotic-based products, including hydrogels, nanorobots, sprays, bandages, masks, protective clothing, also considered. Finally, current challenges future prospects nanozybiotic-related products explored, emphasizing design capabilities for applications.

Язык: Английский

Процитировано

20

A polyene macrolide targeting phospholipids in the fungal cell membrane DOI Creative Commons
Qun Deng, Y. Li,

Wenyan He

и другие.

Nature, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

The global spread of multidrug-resistant pathogenic fungi presents a serious threat to human health, necessitating the discovery antifungals with unique modes action1. However, conventional activity-based screening for previously undescribed antibiotics has been hampered by high-frequency rediscovery known compounds and lack new antifungal targets2. Here we report polyene antibiotic, mandimycin, using phylogeny-guided natural-product platform. Mandimycin is biosynthesized mand gene cluster, evolved in distinct manner from macrolide modified three deoxy sugars. It demonstrated potent broad-spectrum fungicidal activity against wide range fungal pathogens both vitro vivo settings. In contrast that target ergosterol, mandimycin mode action involves targeting various phospholipids cell membranes, resulting release essential ions cells. This ability bind multiple targets gives it robust as well capability evade resistance. identification strategy represents an important advancement uncovering antimicrobial action, which could be developed combat pathogens. Mandimycin, macrolide, exhibits strong possesses other this class.

Язык: Английский

Процитировано

5

Natural Enzyme‐Loaded Polymeric Stealth Coating‐Armed Engineered Probiotics by Disrupting Tumor Lactate Homeostasis to Synergistic Metabolism‐Immuno‐Enzyme Dynamic Therapy DOI Creative Commons

Liuzhou Mao,

Bahriman Xarpidin,

Rui Shi

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Фев. 28, 2025

Abstract Reducing L‐lactate levels in tumors is crucial for alleviating immunosuppression and enhancing treatment efficacy. Recently, bacteria have great potential improving lactate the tumor microenvironment due to their physiological properties, tropism, immunogenicity. However, developing bacterial‐based regulation platforms still facing challenges bacterial modification impacts on activity, macrophage phagocytosis, complex microenvironment. Herein, an engineered Lactobacillus acidophilus (LH@LA) developed, armed with a polymeric stealth coating that co‐loads oxidase (LOx) horseradish peroxidase (HRP). This protects from maintaining activity deep drug delivery. And then, LOx HRP can consume much site through enzyme cascade reaction improve immunosuppressive environment, while causing oxidative stress reduced ATP supply, thereby reversing AKT‐mTOR metabolic pathway activation inhibit growth. More interestingly, LA not only acts as natural enzyme's carrier, but also induces anti‐inflammatory M2 macrophages polarize into pro‐inflammatory M1 by secreting D‐lactate, antitumor immunotherapy. probiotic design provides new idea building safe efficient live delivery platform, providing reference cancer strategies clinical translation prospects.

Язык: Английский

Процитировано

3

Nanozyme-Enhanced Probiotic Spores Regulate the Intestinal Microenvironment for Targeted Acute Gastroenteritis Therapy DOI
Gen Wei, Wanling Liu, Yihong Zhang

и другие.

Nano Letters, Год журнала: 2024, Номер 24(7), С. 2289 - 2298

Опубликована: Фев. 11, 2024

Antibiotic therapeutics to combat intestinal pathogen infections often exacerbate microbiota dysbiosis and impair mucosal barrier functions. Probiotics are promising strategies, because they inhibit colonization improve imbalance. Nevertheless, their limited targeting ability susceptibility oxidative stress have hindered therapeutic potential. To tackle these challenges, Ces3 is synthesized by in situ growth of CeO2 nanozymes with positive charges on probiotic spores, facilitating electrostatic interactions negatively charged pathogens possessing a high reactive oxygen species (ROS) scavenging activity. Importantly, can resist the harsh environment gastrointestinal tract. In mice S. Typhimurium-infected acute gastroenteritis, shows potent anti-S. Typhimurium activity, thereby alleviating dissemination into other organs. Additionally, owing its O2 deprivation capacity, promotes proliferation anaerobic probiotics, reshaping healthy microbiota. This work demonstrates promise combining antibacterial, anti-inflammatory, content regulation properties for gastroenteritis therapy.

Язык: Английский

Процитировано

15

Multifunctional nanozymes for disease diagnosis and therapy DOI Creative Commons
Qingzhi Han, Di Huang, Sijie Li

и другие.

Biomedical Journal, Год журнала: 2024, Номер 47(4), С. 100699 - 100699

Опубликована: Янв. 24, 2024

The development of nanotechnology has brought about groundbreaking advancements in diseases' diagnostics and therapeutics. Among them, multifunctional nanomaterials with enzyme-like activities (i.e., nanozymes) featured high stability, large surface area for bioconjugation, easy storage, offer unprecedented opportunities disease treatment. Recent years have witnessed the great progress nanozyme-based theranostics. To highlight these achievements, this review first introduces recent on nanozymes biosensing diagnostics. Then, it summarizes applications therapeutics including anti-tumor antibacterial treatment, anti-inflammatory other diseases In addition, several targeted strategies to improve therapeutic efficacy nanozyme are discussed. Finally, challenges field diagnosis therapy summarized.

Язык: Английский

Процитировано

10

Review of the Potential of Probiotics in Disease Treatment: Mechanisms, Engineering, and Applications DOI Open Access

Mingkang Liu,

Jinjin Chen,

Ida Putu Wiweka Dharmasiddhi

и другие.

Processes, Год журнала: 2024, Номер 12(2), С. 316 - 316

Опубликована: Фев. 2, 2024

Probiotics, living microorganisms with demonstrated health benefits when administered in sufficient quantities, have a rich history as dietary supplements to benefit human health. Recently, understanding of their mechanisms the gastrointestinal tract has prompted exploration probiotics treating diseases. However, effective and precise delivery remains significant challenge therapeutic applications. Here, we review action most advanced strategies for efficient probiotic delivery. We also discuss potential applications engineered disease treatment. This contributes insights into evolving landscape research

Язык: Английский

Процитировано

10

Nanozymes: an emerging arsenal for the treatment of Candida albicans infection DOI Creative Commons
Yanni Song, Mengyuan Chang, Heng Dong

и другие.

Fundamental Research, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

2