Frontiers in Cellular and Infection Microbiology,
Год журнала:
2024,
Номер
14
Опубликована: Авг. 16, 2024
Background
Vaginal
microbiota
is
involved
in
human
papillomavirus
(HPV)
infection
and
cervical
cancer
(CC)
progression,
the
specific
changes
vaginal
microbial
composition
during
this
process
remains
uncertain.
Objective
This
study
aimed
to
observe
of
microorganisms
different
lesions
identify
biomarkers
at
stages
lesions.
Methods
In
we
used
illumina
high-throughput
gene
sequencing
technology
determine
V4
region
16SrRNA
observed
Results
The
patients
with
high-risk
HPV
significantly
from
that
normal
population,
but
there
no
significant
difference
richness
microbes.
diversity
species
CC
higher
than
or
CIN
patients.
main
manifestation
an
increase
microbes,
a
decrease
relative
abundance
cyanobacteria
Lactobacillus,
dialister,
peptonephila
other
miscellaneous
bacteria.
There
are
characteristic
biomarker
women,
high
risk
detail,
group
was
varibaculum,
saccharopolyspora,
Proteobacteria,
Corynebacterium,
Coprococcus,
Peptococcus
Ruminococcus.
Conclusions
indicated
compositions
microbes
different.
has
certain
diagnostic
effect
on
healthy
These
may
serve
as
potential
for
CC.
It
also
provided
effective
way
treatment
infections
Chinese Journal of Chemistry,
Год журнала:
2024,
Номер
42(13), С. 1515 - 1522
Опубликована: Март 9, 2024
Comprehensive
Summary
We
have
compiled
eight
promising
strategies
for
enhancing
the
specificity
and
selectivity
of
nanozymes,
as
depicted
in
comprehensive
summary
above.
Enzymes
exhibit
intricate
sophisticated
structures,
including
substrate
channels
active
sites,
which
can
inform
design
nanozymes.
Replication
these
structural
features
application
facet
engineering/doping
techniques
significantly
enhance
catalytic
Alternatively,
use
Molecularly
Imprinted
Polymers
(MIPs)
to
coat
nanozymes
represents
an
effective
approach
impart
specificity.
Furthermore,
several
straightforward
stopgap
been
devised
improve
nanozyme
analytical
applications,
such
integration
biorecognition
elements
sensor
arrays
through
surface
modification.
image
Key
Scientists
Advanced Materials,
Год журнала:
2024,
Номер
36(33)
Опубликована: Июнь 14, 2024
Infectious
diseases
caused
by
bacterial,
viral,
and
fungal
pathogens
present
significant
global
health
challenges.
The
rapid
emergence
of
antimicrobial
resistance
exacerbates
this
issue,
leading
to
a
scenario
where
effective
antibiotics
are
increasingly
scarce.
Traditional
antibiotic
development
strategies
proving
inadequate
against
the
swift
evolution
microbial
resistance.
Therefore,
there
is
an
urgent
need
develop
novel
with
mechanisms
distinct
from
those
existing
antibiotics.
Nanozybiotics,
which
nanozyme-based
antimicrobials,
mimic
catalytic
action
lysosomal
enzymes
in
innate
immune
cells
kill
infectious
pathogens.
This
review
reinforces
concept
nanozymes
provides
comprehensive
summary
recent
research
advancements
on
potential
candidates.
Initially,
nanozybiotics
categorized
based
their
activities,
mimicking
either
oxidoreductase-like
or
hydrolase-like
functions,
thereby
highlighting
superior
combating
then
discusses
progress
treating
infections,
confirming
as
translational
nanozybiotic-based
products,
including
hydrogels,
nanorobots,
sprays,
bandages,
masks,
protective
clothing,
also
considered.
Finally,
current
challenges
future
prospects
nanozybiotic-related
products
explored,
emphasizing
design
capabilities
for
applications.
The
global
spread
of
multidrug-resistant
pathogenic
fungi
presents
a
serious
threat
to
human
health,
necessitating
the
discovery
antifungals
with
unique
modes
action1.
However,
conventional
activity-based
screening
for
previously
undescribed
antibiotics
has
been
hampered
by
high-frequency
rediscovery
known
compounds
and
lack
new
antifungal
targets2.
Here
we
report
polyene
antibiotic,
mandimycin,
using
phylogeny-guided
natural-product
platform.
Mandimycin
is
biosynthesized
mand
gene
cluster,
evolved
in
distinct
manner
from
macrolide
modified
three
deoxy
sugars.
It
demonstrated
potent
broad-spectrum
fungicidal
activity
against
wide
range
fungal
pathogens
both
vitro
vivo
settings.
In
contrast
that
target
ergosterol,
mandimycin
mode
action
involves
targeting
various
phospholipids
cell
membranes,
resulting
release
essential
ions
cells.
This
ability
bind
multiple
targets
gives
it
robust
as
well
capability
evade
resistance.
identification
strategy
represents
an
important
advancement
uncovering
antimicrobial
action,
which
could
be
developed
combat
pathogens.
Mandimycin,
macrolide,
exhibits
strong
possesses
other
this
class.
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 28, 2025
Abstract
Reducing
L‐lactate
levels
in
tumors
is
crucial
for
alleviating
immunosuppression
and
enhancing
treatment
efficacy.
Recently,
bacteria
have
great
potential
improving
lactate
the
tumor
microenvironment
due
to
their
physiological
properties,
tropism,
immunogenicity.
However,
developing
bacterial‐based
regulation
platforms
still
facing
challenges
bacterial
modification
impacts
on
activity,
macrophage
phagocytosis,
complex
microenvironment.
Herein,
an
engineered
Lactobacillus
acidophilus
(LH@LA)
developed,
armed
with
a
polymeric
stealth
coating
that
co‐loads
oxidase
(LOx)
horseradish
peroxidase
(HRP).
This
protects
from
maintaining
activity
deep
drug
delivery.
And
then,
LOx
HRP
can
consume
much
site
through
enzyme
cascade
reaction
improve
immunosuppressive
environment,
while
causing
oxidative
stress
reduced
ATP
supply,
thereby
reversing
AKT‐mTOR
metabolic
pathway
activation
inhibit
growth.
More
interestingly,
LA
not
only
acts
as
natural
enzyme's
carrier,
but
also
induces
anti‐inflammatory
M2
macrophages
polarize
into
pro‐inflammatory
M1
by
secreting
D‐lactate,
antitumor
immunotherapy.
probiotic
design
provides
new
idea
building
safe
efficient
live
delivery
platform,
providing
reference
cancer
strategies
clinical
translation
prospects.
Nano Letters,
Год журнала:
2024,
Номер
24(7), С. 2289 - 2298
Опубликована: Фев. 11, 2024
Antibiotic
therapeutics
to
combat
intestinal
pathogen
infections
often
exacerbate
microbiota
dysbiosis
and
impair
mucosal
barrier
functions.
Probiotics
are
promising
strategies,
because
they
inhibit
colonization
improve
imbalance.
Nevertheless,
their
limited
targeting
ability
susceptibility
oxidative
stress
have
hindered
therapeutic
potential.
To
tackle
these
challenges,
Ces3
is
synthesized
by
in
situ
growth
of
CeO2
nanozymes
with
positive
charges
on
probiotic
spores,
facilitating
electrostatic
interactions
negatively
charged
pathogens
possessing
a
high
reactive
oxygen
species
(ROS)
scavenging
activity.
Importantly,
can
resist
the
harsh
environment
gastrointestinal
tract.
In
mice
S.
Typhimurium-infected
acute
gastroenteritis,
shows
potent
anti-S.
Typhimurium
activity,
thereby
alleviating
dissemination
into
other
organs.
Additionally,
owing
its
O2
deprivation
capacity,
promotes
proliferation
anaerobic
probiotics,
reshaping
healthy
microbiota.
This
work
demonstrates
promise
combining
antibacterial,
anti-inflammatory,
content
regulation
properties
for
gastroenteritis
therapy.
Biomedical Journal,
Год журнала:
2024,
Номер
47(4), С. 100699 - 100699
Опубликована: Янв. 24, 2024
The
development
of
nanotechnology
has
brought
about
groundbreaking
advancements
in
diseases'
diagnostics
and
therapeutics.
Among
them,
multifunctional
nanomaterials
with
enzyme-like
activities
(i.e.,
nanozymes)
featured
high
stability,
large
surface
area
for
bioconjugation,
easy
storage,
offer
unprecedented
opportunities
disease
treatment.
Recent
years
have
witnessed
the
great
progress
nanozyme-based
theranostics.
To
highlight
these
achievements,
this
review
first
introduces
recent
on
nanozymes
biosensing
diagnostics.
Then,
it
summarizes
applications
therapeutics
including
anti-tumor
antibacterial
treatment,
anti-inflammatory
other
diseases
In
addition,
several
targeted
strategies
to
improve
therapeutic
efficacy
nanozyme
are
discussed.
Finally,
challenges
field
diagnosis
therapy
summarized.
Processes,
Год журнала:
2024,
Номер
12(2), С. 316 - 316
Опубликована: Фев. 2, 2024
Probiotics,
living
microorganisms
with
demonstrated
health
benefits
when
administered
in
sufficient
quantities,
have
a
rich
history
as
dietary
supplements
to
benefit
human
health.
Recently,
understanding
of
their
mechanisms
the
gastrointestinal
tract
has
prompted
exploration
probiotics
treating
diseases.
However,
effective
and
precise
delivery
remains
significant
challenge
therapeutic
applications.
Here,
we
review
action
most
advanced
strategies
for
efficient
probiotic
delivery.
We
also
discuss
potential
applications
engineered
disease
treatment.
This
contributes
insights
into
evolving
landscape
research
