Vaccine Insights, Год журнала: 2023, Номер 02(08), С. 317 - 321
Опубликована: Авг. 23, 2023
VIEWPOINT "There is interest in the possibility of developing mucosally targeted vaccines hope these may increase mucosal antibody levels, improve protection and reduce shedding virus..."
Язык: Английский
Nature Communications, Год журнала: 2024, Номер 15(1)
Опубликована: Апрель 10, 2024
Several studies have suggested the imprinting of SARS-CoV-2 immunity by original immune challenge without addressing formation de novo response to successive antigen exposures. As this is crucial for development antigenic sin, we assessed against mutated epitopes omicron after vaccine breakthrough. Our data demonstrate a robust humoral in thrice-vaccinated individuals following breakthrough which recall vaccine-induced memory. The and memory B cell responses altered regions surface proteins are impaired. T spike protein present due high cross-reactivity cells rather than response. findings, therefore, underpin speculation that vaccination may lead sin if future variants overcome immunity.
Язык: Английский
Процитировано
10
ACS Nano, Год журнала: 2024, Номер unknown
Опубликована: Сен. 19, 2024
Lipid nanoparticle mRNA vaccines are an exciting but emerging technology used in humans. There is limited understanding of the factors that influence their biodistribution and immunogenicity. Antibodies to poly(ethylene glycol) (PEG), which on surface lipid nanoparticle, detectable humans boosted by human vaccination. We hypothesized PEG-specific antibodies could increase clearance vaccines. To test this, we developed methods quantify both vaccine ionizable frequent serial blood samples from 19 subjects receiving Moderna SPIKEVAX booster immunization. Both peaked 1-2 days post vaccination (median peak level 0.19 3.22 ng mL
Язык: Английский
Процитировано
8
JCI Insight, Год журнала: 2023, Номер 8(18)
Опубликована: Сен. 21, 2023
Understanding mucosal antibody responses from SARS-CoV-2 infection and/or vaccination is crucial to develop strategies for longer term immunity, especially against emerging viral variants. We profiled serial paired and plasma antibodies COVID-19 vaccinated only vaccinees (vaccinated, uninfected), COVID-19-recovered (recovered, vaccinated), individuals with breakthrough Delta or Omicron BA.2 infections infected). Saliva displayed improved antibody-neutralizing activity, Fcγ receptor (FcγR) engagement, IgA levels compared COVID-19-uninfected vaccinees. Furthermore, repeated mRNA boosted SARS-CoV-2-specific IgG2 IgG4 in both mucosa biofluids (saliva tears) plasma; however, these rises negatively correlated FcγR engagement plasma. IgG but not IgA, variants were dampened narrowed by increased preexisting vaccine-induced immunity the ancestral strain. Salivary delayed initiation following infection, BA.2, rose rapidly thereafter. Importantly, salivary engagements enhanced infections. Our data highlight how shapes has implications long-term protection COVID-19.
Язык: Английский
Процитировано
11
Vaccines, Год журнала: 2024, Номер 12(4), С. 352 - 352
Опубликована: Март 25, 2024
Knowledge of the antibody response to third dose inactivated SARS-CoV-2 vaccines is crucial because it subject one largest global vaccination programs. This study integrated microsampling with optical biosensors profile neutralizing antibodies (NAbs) in fifteen vaccinated healthy donors, followed by application machine learning predict at given timepoints. Over a nine-month duration, and venipuncture were conducted seven individual A refined iteration fiber optic biolayer interferometry (FO-BLI) biosensor was designed, enabling rapid multiplexed biosensing NAbs both wild-type Omicron variants minutes. Findings revealed strong correlation (Pearson r 0.919, specificity 100%) between variant NAb levels microsamples sera. Following dose, sera increased 2.9-fold after days 3.3-fold within month, subsequently waning becoming undetectable three months. Considerable but incomplete evasion latest subvariants from booster vaccine-elicited confirmed, although higher number binding (BAbs) identified another FO-BLI Significantly, highly correlated pseudovirus neutralization assay identifying capacities 0.983). Additionally, demonstrated exceptional accuracy predicting levels, an error level <5% for BAbs across multiple Microsample-driven enables individuals access their results hours self-collection, while precise models could guide personalized strategies. The technology’s innate adaptability means has potential effective translation disease prevention vaccine development.
Язык: Английский
Процитировано
4
Cell Reports Medicine, Год журнала: 2024, Номер 5(6), С. 101583 - 101583
Опубликована: Май 22, 2024
Little is known about the effect of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 or SARS2) vaccine breakthrough infections (BTIs) on magnitude and breadth T cell repertoire after exposure to different variants. We studied samples from individuals who experienced symptomatic BTIs during Delta Omicron waves. In pre-BTI samples, 30% donors exhibited substantial immune memory against non-S (spike) SARS2 antigens, consistent with previous undiagnosed asymptomatic infections. Following BTI, we observed (1) enhanced S-specific CD4 CD8 responses in without infection, (2) expansion targets (M, N, nsps) independent variant, (3) generation novel epitopes recognizing variant-specific mutations. These accounted for 9%-15% total epitope repertoire. Overall, boost vaccine-induced by increasing broadening antigens recognized.
Язык: Английский
Процитировано
4
iScience, Год журнала: 2024, Номер 27(7), С. 110283 - 110283
Опубликована: Июнь 15, 2024
The emergence of novel Omicron subvariants has raised concerns regarding the efficacy immunity induced by prior breakthrough infection (BTI) or reinfection against current circulating subvariants. Here, we prospectively investigated durability antibody and T cell responses in individuals post BA.2.2 BTI, with without subsequent BA.5 reinfection. Our findings reveal that emerging subvariants, including CH.1.1, XBB, JN.1, exhibit extensive immune evasion previous infections. Notably, level IgG neutralizing antibodies were found to correlate Fortunately, recognizing both BA.2 CH.1.1 peptides observed. Furthermore, may alleviate imprinting WT-vaccination, bolster virus-specific ICS
Язык: Английский
Процитировано
4
Journal of Clinical Investigation, Год журнала: 2024, Номер 134(17)
Опубликована: Июль 11, 2024
BACKGROUNDThere is uncertainty about the timing of booster vaccination against COVID-19 in highly vaccinated populations during present endemic phase COVID-19. Studies focused on primary have previously suggested improved immunity with a longer interval between first and second vaccine doses.METHODSWe conducted randomized, controlled trial (November 2022-August 2023) assigned 52 fully adults to an immediate or 3-month delayed bivalent Spikevax mRNA vaccine. Follow-up visits were completed for 48 participants (n = 24 per arm), collection saliva plasma samples following each visit.RESULTSThe rise neutralizing antibody responses ancestral Omicron strains almost identical arms. Analyses salivary (IgG, IgA), antibody-dependent phagocytic activity, decay kinetics similar 2 Symptomatic asymptomatic SARS-CoV-2 infections occurred 49% (21 49) over median 11.5 months follow-up also arms.CONCLUSIONSOur data suggest that there was no benefit delaying preimmune COVID-19.TRIAL REGISTRATIONAustralian New Zealand Clinical Trials Registry number 12622000411741 (https://anzctr.org.au/Trial/Registration/TrialReview.aspx?ACTRN=12622000411741).FUNDINGNational Health Medical Research Council, Australia (program grant App1149990) Future Fund (App2005544).
Язык: Английский
Процитировано
4
JCI Insight, Год журнала: 2025, Номер unknown
Опубликована: Янв. 9, 2025
BACKGROUND. The immunogenicity of current influenza vaccines need improvement. Inactivated and COVID-19 mRNA can be co-administered but randomized controlled trial data is lacking on whether the two are more immunogenic if given in same or opposite arms. Murine studies suggest adjuvant when co-formulated delivered together.
Язык: Английский
Процитировано
0
The Journal of Immunology, Год журнала: 2025, Номер unknown
Опубликована: Март 9, 2025
Abstract Human clinical trials have reported immunological outcomes can differ between ipsilateral (same side) and contralateral (alternate sides) prime-boost vaccination. However, our mechanistic understanding of how keeping or shifting the anatomical sites immunization impacts resultant germinal centers (GCs) antibody responses is limited. Here, we use an adjuvanted SARS-CoV-2 spike vaccine to dissect GC dynamics in draining lymph nodes serological following vaccination C57BL/6 mice. Contralateral elicited independent GCs at distinct nodes, where robust secondary only appeared upon distal vaccination, while ongoing from primary site were not boosted. In contrast, resulted sustained activity. Ipsilateral accelerated development titers against ancestral (wild-type [WT]), Beta, BA.1 but later comparable groups terms magnitude, durability, neutralization capacity beyond 28 d. Using a heterologous WT/BA.1 model, cross-reactive generated WT spike, with analogous homologous model. Within B cells, differential recognition antigens was observed further compartmentalized GCs, depending on regimes. Collectively, maintaining common augments kinetics memory cell recall transiently drive higher titers, longer-term are unaffected by localization immunization.
Язык: Английский
Процитировано
0
Emerging Microbes & Infections, Год журнала: 2025, Номер unknown
Опубликована: Апрель 15, 2025
The dynamics of the immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) breakthrough infections remain unclear, particularly when compared responses in naive individuals. In this longitudinal prospective cohort study, 13 participants were recruited. Peripheral blood samples collected every other day until 7 after symptom onset. Transcriptome sequencing, single-cell T-cell receptor (TCR) B-cell (BCR) Olink proteomics, and antigen-antibody binding experiments then performed. During incubation periods infections, peripheral exhibited type cytokine response, which shifted 1 upon Plasma levels C-X-C motif chemokine ligand 10, monocyte chemoattractant protein-1, interferon-γ, interleukin-6 show larger changes than naïve infections. inflammatory rapidly subsided, returning homeostasis by 5 Notably, monocyte-derived S100A8/A9, previously considered a marker disease, physiologically significantly increased early stages mild cases persisted 7, suggesting specific biological function. Longitudinal tracking also revealed that antibodies anti-Receptor Binding Domain (anti-RBD) onset, whereas cytotoxic T lymphocytes appeared 5. This study presents reference for interpreting immunological infectious disease humans.
Язык: Английский
Процитировано
0