Advanced Healthcare Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 19, 2024
Abstract
Recently,
it
has
been
recognized
that
natural
extracellular
matrix
(ECM)
and
tissues
are
viscoelastic,
while
only
elastic
properties
have
investigated
in
the
past.
How
viscoelastic
regulates
stem
cell
patterning
is
critical
for
cell‐ECM
mechano‐transduction.
Here,
this
study
fabricated
different
methacrylated
hyaluronic
acid
(HA)
hydrogels
using
covalent
cross–linking,
consisting
of
two
gels
with
similar
elasticity
(stiffness)
but
viscoelasticity,
viscoelasticity
(stiffness).
Meanwhile,
a
second
set
dual
network
containing
both
coordinated
cross–links.
Human
spinal
cord
organoid
(hSCO)
HA
co‐culture
isogenic
human
blood
vessel
organoids
(hBVOs)
investigated.
The
promote
regional
hSCO
compared
to
hydrogels.
More
can
dorsal
marker
expression,
softer
result
higher
interneuron
expression.
effects
become
more
dominant
than
stiffness
hSCOs
hBVOs.
In
addition,
lead
Yes‐associated
protein
nuclear
translocation,
revealing
mechanism
This
research
provides
insights
into
behaviors
during
ECM‐mimicking
vitro
microenvironments
applications
regenerative
medicine.
Advanced Functional Materials,
Год журнала:
2024,
Номер
34(36)
Опубликована: Апрель 27, 2024
Abstract
An
asymmetrical
wound
dressing
functions
akin
to
human
skin
by
serving
as
a
protective
barrier
between
and
its
immediate
environment.
However,
significant
challenges
persist
concerning
the
robust
adhesion
properties
of
hydrogels,
particularly
when
applied
in
emergency
hemostasis
healing
contexts.
Herein,
study
has
successfully
synthesized
hydrogel
patches
with
Janus
asymmetric‐adhesion,
denoted
HGO‐C,
exclusively
comprised
natural
polymers.
This
achievement
is
realized
through
assembly
adhesive
(HGO)
non‐adhesive
(CGC),
thereby
amalgamating
their
distinct
functionalities.
The
component
served
physical
shield
safeguarding
against
contamination,
while
hydrogel,
contacted
surface,
firmly
adhered
it,
swiftly
arresting
bleeding
facilitating
healing.
Cytocompatibility
tests,
hemolysis
antibacterial
assays,
coagulation
assays
demonstrated
excellent
biocompatibility,
antibacterial,
hemostatic
HGO‐C.
Finally,
vivo
experiments,
including
liver
hemorrhage
assay
assay,
unequivocally
showed
rapid
enhanced
capabilities
Consequently,
these
distinctive
patches,
derived
from
polymers
characterized
asymmetric
properties,
may
have
great
potential
for
real‐life
usage
clinical
patients.
Abstract
The
viscoelasticity
of
mechanically
sensitive
tissues
such
as
periodontal
ligaments
(PDLs)
is
key
in
maintaining
mechanical
homeostasis.
Unfortunately,
PDLs
easily
lose
(e.g.,
stress
relaxation)
during
periodontitis
or
dental
trauma,
which
disrupt
cell–extracellular
matrix
(ECM)
interactions
and
accelerates
tissue
damage.
Here,
Pluronic
F127
diacrylate
(F127DA)
hydrogels
with
PDL‐matched
relaxation
rates
high
elastic
moduli
are
developed.
hydrogel
modulated
without
chemical
cross‐linking
by
controlling
precursor
concentrations.
Under
cytomechanical
loading,
F127DA
fast
significantly
improved
the
fibrogenic
differentiation
potential
PDL
stem
cells
(PDLSCs),
while
cultured
on
various
exhibited
similar
potentials
limited
cell
spreading
traction
forces
under
static
conditions.
Mechanically,
faster‐relaxing
leveraged
loading
to
activate
PDLSC
mechanotransduction
upregulating
integrin–focal
adhesion
kinase
pathway
thus
cytoskeletal
rearrangement,
reinforcing
cell–ECM
interactions.
In
vivo
experiments
confirm
that
promoted
repair
reduced
abnormal
healing
root
resorption
ankyloses)
delayed
replantation
avulsed
teeth.
This
study
firstly
investigated
how
nonlinear
influences
fibrogenesis
PDLSCs
stimuli,
it
reveals
underlying
mechanobiology,
suggests
novel
strategies
for
regeneration.
Advanced Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 19, 2024
Synthetic
extracellular
matrix
(ECM)
mimics
that
can
recapitulate
the
complex
biochemical
and
mechanical
nature
of
native
tissues
are
needed
for
advanced
models
development
disease.
Biomedical
research
has
heavily
relied
on
use
animal-derived
biomaterials,
which
is
now
impeding
their
translational
potential
convoluting
biological
insights
gleaned
from
in
vitro
tissue
models.
Natural
hydrogels
have
long
served
as
a
convenient
effective
cell
culture
tool,
but
advances
materials
chemistry
fabrication
techniques
present
promising
new
avenues
creating
xenogenic-free
ECM
substitutes
appropriate
organotypic
microphysiological
systems.
However,
significant
challenges
remain
synthetic
matrices
approximate
structural
sophistication,
complexity,
dynamic
functionality
tissues.
This
review
summarizes
key
properties
ECM,
discusses
recent
approaches
used
to
systematically
decouple
tune
these
matrices.
The
importance
mechanics,
such
viscoelasticity
plasticity,
also
discussed,
particularly
within
context
organoid
engineered
Emerging
design
strategies
mimic
reviewed,
multi-network
hydrogels,
supramolecular
chemistry,
assembled
monomers.
Carbohydrate Polymers,
Год журнала:
2024,
Номер
338, С. 122173 - 122173
Опубликована: Апрель 17, 2024
The
dynamic
interplay
between
cells
and
their
native
extracellular
matrix
(ECM)
influences
cellular
behavior,
imposing
a
challenge
in
biomaterial
design.
Dynamic
covalent
hydrogels
are
viscoelastic
show
self-healing
ability,
making
them
potential
scaffold
for
recapitulating
ECM
properties.
We
aimed
to
implement
kinetically
thermodynamically
distinct
crosslinkers
prepare
explore
the
arising
properties
effects
on
behavior.
To
do
so,
aldehyde-substituted
hyaluronic
acid
(HA)
was
synthesized
generate
imine,
hydrazone,
oxime
crosslinked
hydrogels.
Differences
equilibrium
constants
of
these
bonds
yielded
including
stiffness,
stress
relaxation,
ability.
degree
substitution
(DS),
polymer
concentration,
crosslinker
aldehyde
ratio,
functionality
hydrogel
were
evaluated.
ability
investigated
samples
same
different
DS
obtain
with
gradient
Subsequently,
human
dermal
fibroblasts
cultured
2D
3D
assess
response
considering
Moreover,
assessing
cell
spreading
morphology
having
similar
modulus
but
relaxation
rates
showed
viscoelasticity
higher
slower
relaxing
Advanced Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 5, 2024
Abstract
The
biochemical
and
biophysical
properties
of
the
extracellular
matrix
(ECM)
play
a
pivotal
role
in
regulating
cellular
behaviors
such
as
proliferation,
migration,
differentiation.
Engineered
protein‐based
hydrogels,
with
highly
tunable
multifunctional
properties,
have
potential
to
replicate
key
features
native
ECM.
Formed
by
self‐assembly
or
crosslinking,
engineered
hydrogels
can
induce
range
cell
through
bioactive
functional
domains
incorporated
into
polymer
backbone.
Using
recombinant
techniques,
amino
acid
sequence
protein
backbone
be
designed
precise
control
over
chain‐length,
folded
structure,
cell‐interaction
sites.
In
this
review,
modular
design
from
both
molecular‐
network‐level
perspective
are
discussed,
summarize
recent
progress
case
studies
highlight
diverse
strategies
used
construct
biomimetic
scaffolds.
This
review
focuses
on
sequences
that
form
structural
blocks,
stimuli‐responsive
blocks
for
scaffold
properties.
Both
physical
chemical
methods
stabilize
dynamic
networks
defined
structure
bioactivity
culture
applications
discussed.
Finally,
discussion
future
directions
scaffolds
is
concluded.
CNS Neuroscience & Therapeutics,
Год журнала:
2025,
Номер
31(1)
Опубликована: Янв. 1, 2025
ABSTRACT
Traumatic
brain
injury
(TBI)
is
one
of
the
most
traumatizing
and
poses
serious
health
risks
to
people's
bodies
due
its
unique
pathophysiological
characteristics.
The
investigations
on
pathological
mechanism
valid
interventions
TBI
have
attracted
widespread
attention
worldwide.
With
bio‐mimic
mechanic
cues,
dynamic
hydrogels
with
stiffness
changes
or
reversible
crosslinking
been
suggested
construct
in
vitro
disease
models
novel
therapeutic
agents
for
TBI.
However,
there
a
lack
clarification
currently
reported
their
biomedical
applications
Our
review
starts
introducing
native
mechanical
characters
then
summarizes
common
chemical
strategies
dynamically
tunable
networks
modeling
therapy.
Finally,
we
prospect
future
development
TBI,
providing
new
insights
guidance
tailored
brain‐targeted
biomaterials.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Май 10, 2024
Lytic
bacteriophages,
viruses
that
lyse
(kill)
bacteria,
hold
great
promise
for
treating
infections,
including
wound
infections
caused
by
antimicrobial-resistant
Biomaterials Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Injectable
hydrogels
have
spawned
great
interest
as
vehicles
for
cell
therapy.
We
find
that
crosslink
strength
controls
the
solid-to-liquid
yielding
transition;
further,
stronger
crosslinks
lead
to
brittle
yielding,
reducing
injected
viability.
