Journal of the American Chemical Society,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 31, 2024
Photochemically
triggered,
transient,
and
temporally
oscillatory-modulated
transcription
machineries
are
introduced.
The
resulting
dynamic
circuits
implemented
to
guide
photochemically
oscillatory
modulation
of
thrombin
toward
temporal
control
over
fibrinogenesis.
One
system
describes
the
assembly
a
reaction
module
leading
triggered
formation
an
active
machinery
that,
in
presence
RNase
H,
guides
transient
activation
A
second
introduces
photochemical
triggering
circuit
consisting
two
coupled
machineries,
depletion
intermediate
product.
concept
is
applied
develop
leads
generation
anti-thrombin
aptamer-modified
oscillating
product
induces
rhythmic
inhibition
thrombin,
accompanied
by
cyclic
deactivation
fibrinogenesis
process.
operation
computational
kinetic
models,
allowing
predict
behaviors
under
different
auxiliary
conditions.
phototriggered
circuit-guided
examined
physiological-like
conditions
within
human
plasma
environment.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 13, 2025
An
entropy-driven
catalysis
(EDC)
strategy
is
appealing
for
amplified
bioimaging
of
microRNAs
in
living
cells;
yet,
complex
operation
procedures,
lacking
cell
selectivity,
and
insufficient
accuracy
hamper
its
further
applications.
Here,
we
introduce
an
ingenious
all-in-one
DNA
nanomachine
(termed
as
AIO-EDN),
which
can
be
triggered
by
endogenous
apurinic/apyrimidinic
endonuclease
1
(APE1)
to
achieve
tumor
cell-selective
dual-mode
imaging
microRNA.
Compared
with
the
traditional
EDC
strategy,
integrated
design
AIO-EDN
achieves
autocatalytic
signal
amplification
without
extra
fuel
strands.
Moreover,
leverages
APE1
overexpressed
cancer
cells
activate
reaction,
which,
however,
exerts
no
target
sensing
activity
normal
cells.
Combining
fluorescence-
surface-enhanced
Raman
scattering
(FL/SERS)
techniques,
this
exhibits
significantly
improved
selectivity
microRNA
This
study
provides
a
new
paradigm
develop
EDC-based
platform
shows
great
potential
in-depth
diagnosis
high
precision.
ACS Synthetic Biology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 2, 2025
We
report
here
the
use
of
antibody-DNA
conjugates
(Ab-DNA)
to
activate
collateral
cleavage
activity
CRISPR-Cas12a
enzyme.
Our
findings
demonstrate
that
Ab-DNA
effectively
trigger
CRISPR-Cas12a,
enabling
transduction
antibody-mediated
recognition
events
into
fluorescence
outputs.
developed
two
different
immunoassays
using
an
as
activator
Cas12a:
CRISPR-based
immunosensing
assay
(CIA)
for
detecting
SARS-CoV-2
spike
S
protein,
which
shows
superior
sensitivity
compared
with
traditional
enzyme-linked
immunosorbent
(ELISA),
and
immunomagnetic
(CIMA).
Notably,
CIMA
successfully
detected
protein
in
undiluted
saliva
a
limit
detection
(LOD)
890
pM
2
h
assay.
results
underscore
benefits
integrating
Cas12a-based
signal
amplification
antibody
methods.
The
potential
conjugates,
combined
CRISPR
technology,
offers
promising
alternative
conventional
enzymes
used
could
facilitate
development
versatile
analytical
platforms
non-nucleic
acid
targets.
Journal of the American Chemical Society,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 11, 2025
Precise
imaging
of
atherosclerotic
plaques
using
biomarkers
could
prompt
the
diagnosis
and
clinical
management
atherosclerosis
(AS)-driven
cardiovascular
diseases.
MicroRNA-155
(miR-155)
plays
a
critical
role
in
AS
development,
with
its
expression
notably
upregulated
foam
cells
within
plaques.
However,
miRNA
methods
for
face
significant
challenges,
including
low
specificity,
inefficient
delivery,
poor
cell
selectivity.
Herein,
we
develop
an
endogenous
hypochlorous
acid
(HClO)-gated
cascade
signal
amplification
strategy
precise
miR-155
living
cells,
enabling
accurate
vivo
ex
detection
This
utilizes
phosphorothioate
(PT)-modified
hairpin
probe
that
is
specifically
deprotected
by
HClO
uncaged
miR-155,
triggering
catalytic
assembly
(CHA)
to
amplify
fluorescence
signals.
The
PT-CHA
probes
are
encapsulated
lipid
nanoparticles
(LNs),
followed
conjugating
phosphatidylserine
(PS)-binding
peptide
(PBP)
selectively
targeting
intensity
PT-CHA@LN-PBP
aorta
region
shows
clear
differentiation
among
AS-bearing
mice,
miR-155-/-
healthy
mice.
Moreover,
strongly
correlates
plaque
area
progression
can
discriminate
vulnerability
risk
under
curve
(AUC)
0.94.
Imaging
human
aortic
tissues
further
validates
probe's
capacity
distinguish
from
normal
endarterium.
These
findings
establish
as
noninvasive,
reliable
diagnostic
tool
assessment
AS.
Frontiers in Cell and Developmental Biology,
Journal Year:
2025,
Volume and Issue:
13
Published: April 7, 2025
MicroRNAs
(miRNAs)
are
small,
non-coding
RNA
molecules
that
play
a
pivotal
role
in
the
post-transcriptional
regulation
of
gene
expression.
Over
past
decade,
they
have
emerged
as
key
regulators
cancer
progression,
influencing
different
cellular
processes
such
proliferation,
apoptosis,
metastasis,
and
immune
evasion.
Their
unique
ability
to
target
multiple
genes
simultaneously
makes
miRNAs
highly
attractive
potential
therapeutic
agents
oncology.
However,
several
challenges
hindered
their
direct
clinical
application,
most
notably
inherent
instability
biological
fluids,
rapid
degradation
by
nucleases,
inefficient
delivery
specific
tumor
sites.
Additionally,
off-target
effects
for
toxicity
further
complicate
use
miRNAs.
Nanomedicine
offers
promising
solution
these
enabling
development
advanced
platforms
stable,
safe,
targeted
Nanoparticle-based
systems,
liposomes,
polymeric
nanoparticles,
inorganic
nanocarriers,
can
protect
from
degradation,
improve
bioavailability,
allow
precise
targeting
through
passive
or
active
mechanisms.
These
nanocarriers
also
be
engineered
release
response
stimuli
within
microenvironment,
enhancing
efficacy
while
minimizing
side
effects.
This
review
will
explore
integration
with
nanotechnology,
focusing
on
various
nanoparticle
formulations
roles
miRNA
stability,
specificity,
function
treatment.
In
addition,
we
discuss
current
advances
preclinical
applications,
highlight
tumor-targeting
strategies,
address
remaining
toxicity,
immunogenicity,
scalability.
Future
research
should
focus
overcoming
barriers,
ultimately
paving
way
widespread
adoption
personalized
miRNA-based
nanomedicine
therapy.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 24, 2025
CRISPR/Cas12a-powered
biosensors
with
guanine
(G)-rich
sequence
reporters
(e.g.,
G-quadruplex
and
G-triplex)
are
widely
used
in
detection
applications
due
to
their
simplicity
sensitivity.
However,
when
these
employed
for
molecular
complex
samples,
they
may
encounter
difficulties
such
as
high
background
signal
susceptibility
interference
because
of
the
"turn-off"
output.
Herein,
we
explore,
first
time,
a
set
phosphorothioate
(ps)-modified
(G4)
G-triplex
(G3)
sequences
that
can
bind
thioflavin
T
(ThT)
an
active
split
CRISPR/Cas12a
system
(SCas12a)
generate
"turn-on"
fluorescent
signal.
To
apply
this
new
phenomenon,
develop
universal
SCas12a-powered
biosensor
nucleic
acid
(miRNA-21)
non-nucleic
(kanamycin)
targets
by
using
ps-modified
hairpin
G3
reporter
(SCas12a/psHG3).
Target
recognition
activates
SCas12a's
trans-cleavage
activity,
leading
cleavage
at
loop
region
psHG3
reporter.
The
released
prelocked
psG3
DNA
binds
ThT
produce
strong
fluorescence
Without
preamplification,
strategy
detect
miRNA-21
limit
100
fM.
Moreover,
SCas12a/psHG3
was
further
utilized
detecting
kanamycin
incorporating
its
aptamers,
enabling
concentrations
low
pM.
This
work
is
system,
showcasing
improved
performance
wide
range
synthetic
biology-based
sensing
technology.
Science Advances,
Journal Year:
2025,
Volume and Issue:
11(20)
Published: May 16, 2025
Synthetic
gene
networks
hold
promise
for
genetic
diagnostics
and
therapy
but
face
limitations
due
to
insufficient
molecular
tools.
Gene-encoded
circular
single-stranded
DNA
(Css
DNA)
has
been
developed
as
a
switchable
vector
enrich
regulatory
components
beyond
protein/RNA-based
systems
in
mammalian
cells.
However,
the
previous
Css
regulator
suffered
from
constrained
sequence
flexibility,
disability
of
multiple-input
multiple-output
(MIMO)
signals,
lack
endogenous
orthogonal
regulation.
Here,
we
address
these
challenges
by
engineering
“bridge”
design
into
regulator.
These
bridges
function
sequence-programmable
switches
control
expression,
responding
signals
(such
ATP,
APE1,
RNase
H)
enabling
trans-regulation
within
or
between
DNAs.
We
exploit
orthogonality
construct
three-input
three-output
circuits.
The
upgraded
DNA–based
strategy
represents
versatile
powerful
platform
regulation
provides
promising
avenue
development
synthetic
networks.
Journal of the American Chemical Society,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 10, 2024
DNA
information
storage
provides
an
excellent
solution
for
metadata
due
to
its
high
density,
programmability,
and
long-term
stability.
However,
current
research
primarily
focuses
on
the
processes
of
storing
reading
data,
lacking
comprehensive
solutions
secure
wiping.
Herein,
we
present
a
method
random
sanitization
in
using
CRISPR-Cas12a
(RSDISC)
based
precise
control
thermodynamic
energy
primer-template
hybridization.
We
utilize
collateral
cleavage
(trans-activity)
single-stranded
(ssDNA)
by
achieve
selective
files
metadata.
This
enables
ssDNA
degradation
with
different
GC
contents,
lengths,
secondary
structures
efficiency
up
99.9%
28,258
oligonucleotides
within
one
round.
demonstrate
that
number
erasable
could
reach
10
