
Cell stem cell, Год журнала: 2022, Номер 29(7), С. 1031 - 1050.e12
Опубликована: Июль 1, 2022
Язык: Английский
Cell stem cell, Год журнала: 2022, Номер 29(7), С. 1031 - 1050.e12
Опубликована: Июль 1, 2022
Язык: Английский
Nature Reviews Genetics, Год журнала: 2022, Номер 23(9), С. 563 - 580
Опубликована: Март 25, 2022
Язык: Английский
Процитировано
625Protein & Cell, Год журнала: 2020, Номер 12(1), С. 7 - 28
Опубликована: Июль 15, 2020
Mammalian fertilization begins with the fusion of two specialized gametes, followed by major epigenetic remodeling leading to formation a totipotent embryo. During development pre-implantation embryo, precise reprogramming progress is prerequisite for avoiding developmental defects or embryonic lethality, but underlying molecular mechanisms remain elusive. For past few years, unprecedented breakthroughs have been made in mapping regulatory network dynamic epigenomes during mammalian early embryo development, taking advantage multiple advances and innovations low-input genome-wide chromatin analysis technologies. The aim this review highlight most recent understanding embryogenesis mammals, including DNA methylation, histone modifications, accessibility 3D organization.
Язык: Английский
Процитировано
161Nature, Год журнала: 2022, Номер 605(7909), С. 315 - 324
Опубликована: Март 21, 2022
Язык: Английский
Процитировано
157Nature Reviews Molecular Cell Biology, Год журнала: 2022, Номер 24(1), С. 6 - 26
Опубликована: Авг. 26, 2022
Язык: Английский
Процитировано
116Nature Communications, Год журнала: 2021, Номер 12(1)
Опубликована: Фев. 23, 2021
Abstract Extensive epigenetic reprogramming occurs during preimplantation embryo development. However, it remains largely unclear how the drastic contributes to transcriptional regulatory network this period. Here, we develop a single-cell multiomics sequencing technology (scNOMeRe-seq) that enables profiling of genome-wide chromatin accessibility, DNA methylation and RNA expression in same individual cell. We apply method depict map mouse find remodeling facilitates reconstruction genetic lineages early embryos. Further, construct zygotic genome activation (ZGA)-associated reveal coordination among multiple layers, transcription factors repeat elements instruct proper ZGA. Cell fates associated cis-regulatory are activated stepwise post-ZGA stages. Trophectoderm (TE)-specific play dual roles promoting TE program while repressing inner cell mass (ICM) ICM/TE separation.
Язык: Английский
Процитировано
114Nature Genetics, Год журнала: 2021, Номер 53(4), С. 551 - 563
Опубликована: Апрель 1, 2021
Язык: Английский
Процитировано
114Science, Год журнала: 2022, Номер 378(6615)
Опубликована: Сен. 8, 2022
Translational regulation plays a critical role during the oocyte-to-embryo transition (OET) and zygotic genome activation (ZGA). Here, we integrated ultra-low-input ribosome profiling (Ribo-lite) with messenger RNA sequencing to co-profile translatome transcriptome in human oocytes early embryos. Comparison mouse counterparts identified widespread differentially translated gene functioning epigenetic reprogramming, transposon defense, small biogenesis, part driven by species-specific regulatory elements 3' untranslated regions. Moreover, PRD-like homeobox transcription factors, including TPRXL, TPRX1, TPRX2, are highly around ZGA. TPRX1/2/L knockdown leads defective ZGA preimplantation development. Ectopically expressed TPRXs bind activate key genes embryonic stem cells. These data reveal conservation divergence of translation landscapes OET identify regulators
Язык: Английский
Процитировано
110Science, Год журнала: 2022, Номер 378(6626), С. 1305 - 1315
Опубликована: Ноя. 24, 2022
Life begins with a switch in genetic control from the maternal to embryonic genome during zygotic activation (ZGA). Despite its importance, essential regulators of ZGA remain largely unknown mammals. On basis de novo motif searches, we identified orphan nuclear receptor Nr5a2 as key activator major mouse two-cell embryos. is required for progression beyond stage. It binds within SINE B1/Alu retrotransposable elements found cis-regulatory regions genes. Chemical inhibition suggests that 72% genes are regulated by and potentially other receptors. promotes chromatin accessibility nucleosomal DNA vitro. We conclude an pioneer factor regulates ZGA.
Язык: Английский
Процитировано
103Cell stem cell, Год журнала: 2022, Номер 29(7), С. 1051 - 1066.e8
Опубликована: Июль 1, 2022
Язык: Английский
Процитировано
72Molecular Cell, Год журнала: 2022, Номер 82(5), С. 986 - 1002.e9
Опубликована: Фев. 18, 2022
Язык: Английский
Процитировано
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