Histone post-translational modifications — cause and consequence of genome function

Nature Reviews Genetics, Journal Year: 2022, Volume and Issue: 23(9), P. 563 - 580

Published: March 25, 2022

Language: Английский

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Insights into epigenetic patterns in mammalian early embryos

Protein & Cell, Journal Year: 2020, Volume and Issue: 12(1), P. 7 - 28

Published: July 15, 2020

Mammalian fertilization begins with the fusion of two specialized gametes, followed by major epigenetic remodeling leading to formation a totipotent embryo. During development pre-implantation embryo, precise reprogramming progress is prerequisite for avoiding developmental defects or embryonic lethality, but underlying molecular mechanisms remain elusive. For past few years, unprecedented breakthroughs have been made in mapping regulatory network dynamic epigenomes during mammalian early embryo development, taking advantage multiple advances and innovations low-input genome-wide chromatin analysis technologies. The aim this review highlight most recent understanding embryogenesis mammals, including DNA methylation, histone modifications, accessibility 3D organization.

Language: Английский

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Rolling back human pluripotent stem cells to an eight-cell embryo-like stage

Nature, Journal Year: 2022, Volume and Issue: 605(7909), P. 315 - 324

Published: March 21, 2022

Language: Английский

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Regulation, functions and transmission of bivalent chromatin during mammalian development

Nature Reviews Molecular Cell Biology, Journal Year: 2022, Volume and Issue: 24(1), P. 6 - 26

Published: Aug. 26, 2022

Language: Английский

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Single-cell multiomics sequencing reveals the functional regulatory landscape of early embryos

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: Feb. 23, 2021

Abstract Extensive epigenetic reprogramming occurs during preimplantation embryo development. However, it remains largely unclear how the drastic contributes to transcriptional regulatory network this period. Here, we develop a single-cell multiomics sequencing technology (scNOMeRe-seq) that enables profiling of genome-wide chromatin accessibility, DNA methylation and RNA expression in same individual cell. We apply method depict map mouse find remodeling facilitates reconstruction genetic lineages early embryos. Further, construct zygotic genome activation (ZGA)-associated reveal coordination among multiple layers, transcription factors repeat elements instruct proper ZGA. Cell fates associated cis-regulatory are activated stepwise post-ZGA stages. Trophectoderm (TE)-specific play dual roles promoting TE program while repressing inner cell mass (ICM) ICM/TE separation.

Language: Английский

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Distinct dynamics and functions of H2AK119ub1 and H3K27me3 in mouse preimplantation embryos

Nature Genetics, Journal Year: 2021, Volume and Issue: 53(4), P. 551 - 563

Published: April 1, 2021

Language: Английский

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Translatome and transcriptome co-profiling reveals a role of TPRXs in human zygotic genome activation

Science, Journal Year: 2022, Volume and Issue: 378(6615)

Published: Sept. 8, 2022

Translational regulation plays a critical role during the oocyte-to-embryo transition (OET) and zygotic genome activation (ZGA). Here, we integrated ultra-low-input ribosome profiling (Ribo-lite) with messenger RNA sequencing to co-profile translatome transcriptome in human oocytes early embryos. Comparison mouse counterparts identified widespread differentially translated gene functioning epigenetic reprogramming, transposon defense, small biogenesis, part driven by species-specific regulatory elements 3' untranslated regions. Moreover, PRD-like homeobox transcription factors, including TPRXL, TPRX1, TPRX2, are highly around ZGA. TPRX1/2/L knockdown leads defective ZGA preimplantation development. Ectopically expressed TPRXs bind activate key genes embryonic stem cells. These data reveal conservation divergence of translation landscapes OET identify regulators

Language: Английский

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Zygotic genome activation by the totipotency pioneer factor Nr5a2

Science, Journal Year: 2022, Volume and Issue: 378(6626), P. 1305 - 1315

Published: Nov. 24, 2022

Life begins with a switch in genetic control from the maternal to embryonic genome during zygotic activation (ZGA). Despite its importance, essential regulators of ZGA remain largely unknown mammals. On basis de novo motif searches, we identified orphan nuclear receptor Nr5a2 as key activator major mouse two-cell embryos. is required for progression beyond stage. It binds within SINE B1/Alu retrotransposable elements found cis-regulatory regions genes. Chemical inhibition suggests that 72% genes are regulated by and potentially other receptors. promotes chromatin accessibility nucleosomal DNA vitro. We conclude an pioneer factor regulates ZGA.

Language: Английский

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Stage-specific H3K9me3 occupancy ensures retrotransposon silencing in human pre-implantation embryos

Cell stem cell, Journal Year: 2022, Volume and Issue: 29(7), P. 1051 - 1066.e8

Published: July 1, 2022

Language: Английский

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The landscape of pioneer factor activity reveals the mechanisms of chromatin reprogramming and genome activation

Molecular Cell, Journal Year: 2022, Volume and Issue: 82(5), P. 986 - 1002.e9

Published: Feb. 18, 2022

Language: Английский

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