Target DNA-dependent activation mechanism of the prokaryotic immune system SPARTA DOI Creative Commons
Giada Finocchio, Balwina Koopal, Ana Potocnik

и другие.

Nucleic Acids Research, Год журнала: 2024, Номер 52(4), С. 2012 - 2029

Опубликована: Янв. 15, 2024

In both prokaryotic and eukaryotic innate immune systems, TIR domains function as NADases that degrade the key metabolite NAD+ or generate signaling molecules. Catalytic activation of requires oligomerization, but how this is achieved varies in distinct systems. Short Argonaute (pAgo)/TIR-APAZ (SPARTA) system, NADase activity triggered upon guide RNA-mediated recognition invading DNA by an unknown mechanism. Here, we describe cryo-EM structures SPARTA inactive monomeric target DNA-activated tetrameric states. The structure reveals absence DNA, a C-terminal tail TIR-APAZ occupies nucleic acid binding cleft formed pAgo subunits, inhibiting activation. active complex, displaces induces conformational changes facilitate SPARTA-SPARTA dimerization. Concurrent release rotation one domain allow it to form composite catalytic site with other within dimer, self-complementary interface mediates cooperative tetramerization. Combined, study provides critical insights into structural architecture molecular mechanism underlying DNA-dependent oligomerization

Язык: Английский

Structure, biochemical function, and signaling mechanism of plant NLRs DOI Creative Commons
Jizong Wang, Wen Song, Jijie Chai

и другие.

Molecular Plant, Год журнала: 2022, Номер 16(1), С. 75 - 95

Опубликована: Ноя. 22, 2022

To counter pathogen invasion, plants have evolved a large number of immune receptors, including membrane-resident pattern recognition receptors (PRRs) and intracellular nucleotide-binding leucine-rich repeat (NLRs). Our knowledge about PRR NLR signaling mechanisms has expanded significantly over the past few years. Plant NLRs form multi-protein complexes called resistosomes in response to effectors, mediated by converges on Ca2+-permeable channels. channels important for also been identified. These findings highlight crucial role Ca2+ triggering plant signaling. In this review, we first discuss structural biochemical non-canonical then summarize our immune-related their roles We potential intricate interaction between

Язык: Английский

Процитировано

53
Small holes, big impact: Stomata in plant–pathogen–climate epic trifecta DOI Creative Commons
Shuguo Hou, Olivier Rodrigues, Zunyong Liu

и другие.

Molecular Plant, Год журнала: 2023, Номер 17(1), С. 26 - 49

Опубликована: Дек. 1, 2023

Язык: Английский

Процитировано

35
Structural insights into mechanisms of Argonaute protein-associated NADase activation in bacterial immunity DOI Creative Commons
Xiaoshen Wang, Xuzichao Li, Guimei Yu

и другие.

Cell Research, Год журнала: 2023, Номер 33(9), С. 699 - 711

Опубликована: Июнь 13, 2023

Nicotinamide adenine dinucleotide (NAD+) is a central metabolite in cellular processes. Depletion of NAD+ has been demonstrated to be prevalent theme both prokaryotic and eukaryotic immune responses. Short Argonaute proteins (Agos) are associated with NADase domain-containing (TIR-APAZ or SIR2-APAZ) encoded the same operon. They confer immunity against mobile genetic elements, such as bacteriophages plasmids, by inducing depletion upon recognition target nucleic acids. However, molecular mechanisms underlying activation NADase/Ago systems remain unknown. Here, we report multiple cryo-EM structures complexes from two distinct (TIR-APAZ/Ago SIR2-APAZ/Ago). Target DNA binding triggers tetramerization TIR-APAZ/Ago complex cooperative self-assembly mechanism, while heterodimeric SIR2-APAZ/Ago does not assemble into higher-order oligomers binding. activities these unleashed via similar closed-to-open transition catalytic pocket, albeit different mechanisms. Furthermore, functionally conserved sensor loop employed inspect guide RNA-target base pairing facilitate conformational rearrangement Ago required for systems. Overall, our study reveals mechanistic diversity similarity protein-associated response.

Язык: Английский

Процитировано

31
Pangenomic analysis reveals plant NAD + manipulation as an important virulence activity of bacterial pathogen effectors DOI Creative Commons
Michelle T. Hulin, Lionel Hill, Jonathan D. G. Jones

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2023, Номер 120(7)

Опубликована: Фев. 8, 2023

Nicotinamide adenine dinucleotide (NAD + ) has emerged as a key component in prokaryotic and eukaryotic immune systems. The recent discovery that Toll/interleukin-1 receptor (TIR) proteins function NAD hydrolases (NADase) links -derived small molecules with signaling. We investigated pathogen manipulation of host metabolism virulence strategy. Using the pangenome model bacterial Pseudomonas syringae , we conducted structure-based similarity search from 35,000 orthogroups for type III effectors (T3Es) potential NADase activity. Thirteen T3Es, including five newly identified candidates, were possess domain(s) characteristic seven -hydrolyzing enzyme families. Most strains depend on secretion system to cause disease, encode at least one -manipulating T3E, many have several. experimentally confirmed III-dependent novel named HopBY, which shows structural both TIR adenosine diphosphate ribose (ADPR) cyclase. Homologs HopBY predicted be VI diverse species, indicating recruitment this activity by microbial secreted during various interspecies interactions. efficiently hydrolyzes specifically produces 2′cADPR, can also produced receptors plants other bacteria. Intriguingly, effector promoted virulence, 2′cADPR may not signaling molecule directly initiates immunity. This study highlights host-pathogen battleground centered around provides insight into involved plant

Язык: Английский

Процитировано

30
Assembly and Architecture of NLR Resistosomes and Inflammasomes DOI Creative Commons
Zehan Hu, Jijie Chai

Annual Review of Biophysics, Год журнала: 2023, Номер 52(1), С. 207 - 228

Опубликована: Янв. 10, 2023

Nucleotide-binding and leucine-rich repeat (NLR) proteins are critical intracellular immune receptors in both animals plants. Perception of pathogen-derived or stress-associated signals induces NLR oligomerization to form multiprotein complexes called inflammasomes resistosomes plants mediate host response. Significant progress has been made during the past few years our understanding biology, particularly structural perspective these two types NLR-containing complexes. In this article, we review latest advances knowledge how activated assembled information provides insight into their distinct mechanisms action. Commonalities differences between also discussed.

Язык: Английский

Процитировано

28
TIR-domain enzymatic activities at the heart of plant immunity DOI Creative Commons
Federica Locci, Junli Wang, Jane E. Parker

и другие.

Current Opinion in Plant Biology, Год журнала: 2023, Номер 74, С. 102373 - 102373

Опубликована: Май 5, 2023

Toll/interleukin-1/resistance (TIR) domain proteins contribute to innate immunity in all cellular kingdoms. TIR modules are activated by self-association and plants, mammals bacteria, some TIRs have enzymatic functions that crucial for disease resistance and/or cell death. Many plant TIR-only pathogen effector-activated TIR-domain NLR receptors NAD+ hydrolysing enzymes. Biochemical, structural functional studies established both TIR-protein types, certain bacterial TIRs, NADase activity generates bioactive signalling intermediates which promote resistance. A set of TIR-catalysed nucleotide isomers was discovered bind activate EDS1 complexes, promoting their interactions with co-functioning helper NLRs. Analysis enzymes across kingdoms fills an important gap understanding how disturbance induces TIR-regulated immune responses.

Язык: Английский

Процитировано

26
New Biochemical Principles for NLR Immunity in Plants DOI Creative Commons
Jijie Chai, Wen Song, Jane E. Parker

и другие.

Molecular Plant-Microbe Interactions, Год журнала: 2023, Номер 36(8), С. 468 - 475

Опубликована: Авг. 1, 2023

While working for the United States Department of Agriculture on North Dakota Agricultural College campus in Fargo, Dakota, 1940s and 1950s, Harold H. Flor formulated genetic principles coevolving plant host-pathogen interactions that govern disease resistance or susceptibility. His ‘gene-for-gene’ legacy runs deep modern pathology continues to inform molecular models immune recognition signaling. In this review, we discuss recent biochemical insights immunity conferred by nucleotide-binding domain/leucine-rich-repeat (NLR) receptors, which are major gene-for-gene determinants nature cultivated crops. Structural analyses pathogen-activated NLR oligomers (resistosomes) reveal how different subtypes converge various ways calcium (Ca 2+ ) signaling promote pathogen host cell death. Especially striking is identification nucleotide-based signals generated enzymatically toll-interleukin 1 receptor (TIR) domain NLRs. These small molecules part an emerging family TIR-produced cyclic noncyclic nucleotide steer cell-death responses bacteria, mammals, plants. A combined genetic, molecular, understanding activation provides exciting new opportunities combatting diseases [Formula: see text] Copyright © 2023 The Author(s). This open access article distributed under CC BY-NC-ND 4.0 International license .

Язык: Английский

Процитировано

26
Nucleic-acid-triggered NADase activation of a short prokaryotic Argonaute DOI
Xiaopan Gao, Kun Shang, Kaixiang Zhu

и другие.

Nature, Год журнала: 2023, Номер 625(7996), С. 822 - 831

Опубликована: Окт. 2, 2023

Язык: Английский

Процитировано

25
Multiple enzymatic activities of a Sir2-HerA system cooperate for anti-phage defense DOI Creative Commons
Dongmei Tang, Yijun Chen, Hao Chen

и другие.

Molecular Cell, Год журнала: 2023, Номер 83(24), С. 4600 - 4613.e6

Опубликована: Дек. 1, 2023

Язык: Английский

Процитировано

25
TIR domains produce histidine-ADPR conjugates as immune signaling molecules in bacteria DOI Creative Commons

Dziugas Sabonis,

Carmel Avraham, Allen Lu

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 3, 2024

Abstract TIR domains are central components of pattern recognition immune proteins across all life. In both bacteria and plants, TIR-domain were shown to recognize pathogen invasion then produce signaling molecules exclusively comprising nucleotide moieties. Here we show that the domain protein type II Thoeris defense system in produces a unique molecule amino acid histidine conjugated ADP-ribose (His-ADPR). His-ADPR is generated response phage infection activates cognate effector by binding Macro located at C-terminus protein. By determining crystal structure ligand-bound domain, describe structural basis for recognition. Our analyses furthermore reveal family bind sequester molecules, allowing phages evade TIR- mediated immunity. These data demonstrate diversity bacterial new class TIR-derived combining

Язык: Английский

Процитировано

15