Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Март 15, 2024
Dengue
has
had
a
significant
global
health
impact,
with
dramatic
increase
in
incidence
over
the
past
50
years,
affecting
more
than
100
countries.
The
absence
of
specific
treatment
or
widely
applicable
vaccine
emphasizes
urgent
need
for
innovative
strategies.
This
perspective
reevaluates
current
evidence
supporting
concept
dual
protection
against
dengue
virus
(DENV)
through
natural
antibodies
(NAbs),
particularly
anti-α-Gal
induced
by
host’s
gut
microbiome
(GM).
These
serve
purpose.
Firstly,
they
can
directly
identify
DENV,
as
mosquito-derived
viral
particles
have
been
observed
to
carry
α-Gal,
thereby
providing
safeguard
human
infections.
Secondly,
possess
potential
impede
development
vector
interacting
vector’s
and
triggering
infection-refractory
states.
intricate
interplay
between
GM
NAbs
on
one
side
DENV
other
suggests
novel
approach,
using
target
simultaneously
disrupt
decrease
pathogen
transmission
competence,
blocking
cycles.
The
human
gut
microbiome
plays
an
important
role
in
resisting
colonization
of
the
host
by
pathogens,
but
we
lack
ability
to
predict
which
communities
will
be
protective.
We
studied
how
bacteria
influence
two
major
bacterial
both
vitro
and
gnotobiotic
mice.
Whereas
single
species
alone
had
negligible
effects,
resistance
greatly
increased
with
community
diversity.
Moreover,
this
community-level
rested
critically
upon
certain
being
present.
explained
these
ecological
patterns
through
collective
resistant
consume
nutrients
that
overlap
those
used
pathogen.
Furthermore,
applied
our
findings
successfully
resist
a
novel
target
strain.
Our
work
provides
reason
why
diversity
is
beneficial
suggests
route
for
rational
design
pathogen-resistant
communities.
Immunity,
Год журнала:
2024,
Номер
57(4), С. 613 - 631
Опубликована: Апрель 1, 2024
SummaryWhile
largely
neglected
over
decades
during
which
adaptive
immunity
captured
most
of
the
attention,
innate
immune
mechanisms
have
now
become
central
to
our
understanding
immunology.
Innate
provides
first
barrier
infection
in
vertebrates,
and
it
is
sole
mechanism
host
defense
invertebrates
plants.
also
plays
a
critical
role
maintaining
homeostasis,
shaping
microbiota,
disease
contexts
such
as
cancer,
neurodegeneration,
metabolic
syndromes,
aging.
The
emergence
field
has
led
an
expanded
view
system,
no
longer
restricted
vertebrates
instead
concerns
all
metazoans,
plants,
even
prokaryotes.
study
given
rise
new
concepts
language.
Here,
we
review
history
definition
core
immunity,
discussing
their
value
fruitfulness
long
run.
Many
species,
including
humans,
host
communities
of
symbiotic
microbes.
There
is
a
vast
literature
on
the
ways
these
microbiomes
affect
hosts,
but
here
we
argue
for
an
increased
focus
how
hosts
their
microbiomes.
Hosts
exert
control
over
symbionts
through
diverse
mechanisms,
immunity,
barrier
function,
physiological
homeostasis,
and
transit.
These
mechanisms
enable
to
shape
ecology
evolution
generate
natural
selection
microbial
traits
that
benefit
host.
Our
result
from
perpetual
tension
between
symbiont
evolution,
can
leverage
host's
evolved
abilities
regulate
microbiota
prevent
treat
disease.
The
study
will
be
central
our
ability
both
understand
manipulate
microbiotas
better
health.
Persistent
colonization
and
outgrowth
of
potentially
pathogenic
organisms
in
the
intestine
can
result
from
long-term
antibiotic
use
or
inflammatory
conditions,
may
perpetuate
dysregulated
immunity
tissue
damage
Ulcerative
colitis
(UC)
is
often
accompanied
by
intestinal
inflammation
and
disruption
of
epithelial
structures,
which
are
closely
associated
with
changes
in
the
microbiota.
We
previously
revealed
that
Min
pigs,
a
native
Chinese
breed,
more
resistant
to
dextran
sulfate
sodium
(DSS)-induced
than
commercial
Yorkshire
pigs.
Characterizing
microbiota
pigs
would
allow
identification
core
microbes
confer
resistance.
By
analyzing
linked
disease
course
we
observed
The
widespread
selective
pressure
of
antibiotics
in
the
environment
has
led
to
propagation
antibiotic
resistance
genes
(ARGs).
However,
mechanisms
by
which
microbes
balance
population
growth
with
enrichment
ARGs
remain
poorly
understood.
To
address
this,
we
employed
microcosm
cultivation
at
different
(i.e.,
Oxytetracycline,
OTC)
stresses
across
concentrations
from
environmental
clinical.
Paired
shot-gun
metagenomics
analysis
and
quantification
bacterial
growth,
trait-based
assessment
soil
microbiota
was
applied
reveal
association
between
key
ARG
subtypes,
representative
taxa,
functional-gene
features
that
drive
ARGs.
Our
results
illuminate
resistome
variation
is
closely
associated
growth.
A
non-monotonic
change
abundance
richness
observed
over
a
concentration
gradient
none
10
mg/l.
Soil
exposed
intermediate
OTC
0.1
0.5
mg/l)
showed
greater
increases
total
Community
compositionally,
i.e.,
Pseudomonadaceae
considered
boost
increase
It
chromosomally
carried
kinds
multidrug
such
as
mexAB-oprM
mexCD-oprJ
could
mediate
intrinsic
OTC.
Streptomycetaceae
shown
better
adaptive
ability
than
other
clinical
concentrations.
it
contributed
less
represents
stress-tolerant
lifestyle
grows
slowly
carries
fewer
In
terms
community
genetic
features,
aggregated
traits
further
indicates
enhancement
resource
acquisition
yield
driving
abundance.
Moreover,
optimizations
energy
production
conversion,
alongside
streamlining
bypass
metabolic
pathways,
sub-inhibitory
conditions.
this
study
suggest
competitive
lifestyles
are
selected
under
stress
nutrient
scarcity.
They
possess
substrate
utilization
capacity
carry
more
ARGs,
due
they
were
faster
growing
leading
This
expanded
application
assessments
understanding
ecology
propagation.
And
finding
illustrated
changes
accompanied
switching
microbiome,
theoretically
supports
control
approach
based
on
principle
species
exclusion.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Март 22, 2024
Abstract
Microbiome
engineering
–
the
targeted
manipulation
of
microbial
communities
is
considered
a
promising
strategy
to
restore
ecosystems,
but
experimental
support
and
mechanistic
understanding
are
required.
Here,
we
show
that
bacterial
inoculants
for
soil
microbiome
may
fail
establish
because
they
inadvertently
facilitate
growth
native
resident
microbiomes.
By
generating
microcosms
in
presence
or
absence
standardized
communities,
how
different
nutrient
availabilities
limit
outgrowth
focal
(three
Pseudomonads),
this
might
be
improved
by
adding
an
artificial,
inoculant-selective
niche.
Through
random
paired
interaction
assays
agarose
micro-beads,
demonstrate
that,
addition
direct
competition,
lose
competitiveness
facilitating
bacteria.
Metatranscriptomics
experiments
with
toluene
as
selective
niche
inoculant
Pseudomonas
veronii
indicate
facilitation
due
loss
uptake
excreted
metabolites
taxa.
Generation
niches
help
favor
their
proliferation
duration
intended
action
while
limiting
competitive
loss.
Nature Microbiology,
Год журнала:
2024,
Номер
9(7), С. 1792 - 1811
Опубликована: Июнь 11, 2024
The
Klebsiella
oxytoca
species
complex
is
part
of
the
human
microbiome,
especially
during
infancy
and
childhood.
K.
strains
can
produce
enterotoxins,
namely,
tilimycin
tilivalline,
while
also
contributing
to
colonization
resistance
(CR).
relationship
between
these
seemingly
contradictory
roles
not
well
understood.
Here,
by
coupling
ex
vivo
assays
with
CRISPR-mutagenesis
various
mouse
models,
we
show
that
provides
CR
against
Salmonella
Typhimurium.
In
vitro,
antimicrobial
activity
depended
on
production
was
induced
simple
carbohydrates.
vivo,
toxin
in
germ-free
mice,
it
largely
toxin-independent
mice
residual
microbiota.
This
linked
relative
levels
toxin-inducing
carbohydrates
vivo.
Finally,
dulcitol
utilization
essential
for
gnotobiotic
mice.
Together,
this
demonstrates
nutrient
availability
key
both
toxin-dependent
substrate-driven
competition
Salmonella.