Optical sectioning methods in three-dimensional bioimaging

Light Science & Applications, Год журнала: 2025, Номер 14(1)

Опубликована: Янв. 1, 2025

Abstract In recent advancements in life sciences, optical microscopy has played a crucial role acquiring high-quality three-dimensional structural and functional information. However, the quality of 3D images is often compromised due to intense scattering effect biological tissues, compounded by several issues such as limited spatiotemporal resolution, low signal-to-noise ratio, inadequate depth penetration, high phototoxicity. Although various sectioning techniques have been developed address these challenges, each method adheres distinct imaging principles for specific applications. As result, effective selection suitable across diverse scenarios become yet challenging. This paper comprehensively overviews existing guidance under different scenarios. Specifically, we categorize microscope design based on spatial relationship between illumination detection axis, i.e., on-axis off-axis. classification provides unique perspective compare implementation performances approaches. Lastly, integrate selected methods custom-built off-axis system present future development techniques.

Язык: Английский

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Projectome-based characterization of hypothalamic peptidergic neurons in male mice

Nature Neuroscience, Год журнала: 2025, Номер unknown

Опубликована: Март 26, 2025

Язык: Английский

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An integrated single-nucleus and spatial transcriptomics atlas reveals the molecular landscape of the human hippocampus

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 28, 2024

Abstract The hippocampus contains many unique cell types, which serve the structure’s specialized functions, including learning, memory and cognition. These cells have distinct spatial topography, morphology, physiology, connectivity, highlighting need for transcriptome-wide profiling strategies that retain cytoarchitectural organization. Here, we generated spatially-resolved transcriptomics (SRT) single-nucleus RNA-sequencing (snRNA-seq) data from adjacent tissue sections of anterior human across ten adult neurotypical donors. We defined molecular profiles hippocampal types domains. Using non-negative matrix factorization transfer integrated these to define gene expression patterns within snRNA-seq infer in SRT data. With this approach, leveraged existing rodent datasets feature information on circuit connectivity neural activity induction make predictions about axonal projection targets likelihood ensemble recruitment spatially-defined cellular populations hippocampus. Finally, genome-wide association studies with transcriptomic identify enrichment genetic components neurodevelopmental, neuropsychiatric, neurodegenerative disorders domains, To comprehensive atlas accessible scientific community, both raw processed are freely available, through interactive web applications.

Язык: Английский

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Hippocampal Neuroanatomy

Oxford University Press eBooks, Год журнала: 2025, Номер unknown, С. 37 - 120

Опубликована: Янв. 1, 2025

Abstract This chapter provides an overview of the neurons and intrinsic extrinsic connections rodent, nonhuman primate, human hippocampal formation. While hippocampus related cortical regions have many same excitatory inhibitory as other regions, it also has at least a few neuron types, like mossy cell, that do not occur in areas. What is most distinctive about formation organization its connections, which are generally, though exclusively, unidirectional. there similarities features formation, substantive differences.

Язык: Английский

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Connecto-informatics at the mesoscale: current advances in image processing and analysis for mapping the brain connectivity

Brain Informatics, Год журнала: 2024, Номер 11(1)

Опубликована: Июнь 4, 2024

Abstract Mapping neural connections within the brain has been a fundamental goal in neuroscience to understand better its functions and changes that follow aging diseases. Developments imaging technology, such as microscopy labeling tools, have allowed researchers visualize this connectivity through high-resolution brain-wide imaging. With this, image processing analysis become more crucial. However, despite wealth of images generated, access an integrated pipeline process these data is challenging due scattered information on available tools methods. To map connections, registration atlases feature extraction segmentation signal detection are necessary. In review, our provide updated overview recent advances image-processing methods, with particular focus fluorescent mouse brain. Our outline pathway toward tailored for connecto-informatics. An workflow will facilitate researchers’ approach mapping complex networks their underlying functions. By highlighting fluroscent brain, review contribute deeper grasp connecto-informatics, paving way comprehension implications.

Язык: Английский

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Human hippocampal CA3 uses specific functional connectivity rules for efficient associative memory

Cell, Год журнала: 2024, Номер unknown

Опубликована: Дек. 1, 2024

Язык: Английский

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Study of tree shrew biology and models: A booming and prosperous field for biomedical research

动物学研究, Год журнала: 2024, Номер 45(4), С. 877 - 909

Опубликована: Янв. 1, 2024

The tree shrew (Tupaia belangeri) has long been proposed as a suitable alternative to non-human primates (NHPs) in biomedical and laboratory research due its close evolutionary relationship with primates. In recent years, significant advances have facilitated studies, including the determination of genome, genetic manipulation using spermatogonial stem cells, viral vector-mediated gene delivery, mapping brain atlas. However, limited availability shrews globally remains substantial challenge field. Additionally, determining key questions best answered constitutes another difficulty. Tree models historically used study hepatitis B virus (HBV) C (HCV) infection, myopia, psychosocial stress-induced depression, more studies focusing on developing animal for infectious neurodegenerative diseases. Despite these efforts, impact not yet matched that rodent or NHP research. This review summarizes prominent advancements reflects biological addressed this model. We emphasize intensive dedication robust international collaboration are essential achieving breakthroughs studies. use unique resource is expected gain considerable attention application advanced techniques development viable models, meeting increasing demands life science

Язык: Английский

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The interhemispheric amygdala-accumbens circuit encodes negative valence in mice

Science, Год журнала: 2024, Номер 386(6722)

Опубликована: Ноя. 7, 2024

The structurally symmetric mammalian brain hemispheres are interconnected by commissural axons across the midline. However, functions of interhemispheric connectivity remain largely unknown. We found that in mice, transection anterior commissure (AC), which connects rostroventral forebrain, impaired avoidant behaviors. basolateral amygdala (BLA) mouse projects to contralateral nucleus accumbens (NAc) through AC, independent its ipsilateral projections. Aversive stimuli activated BLA-NAc Positive stimuli, however, Selective activation projections D2-positive medium spiny neurons (D2-MSNs), reduced NAc dopamine levels, and caused aversion, whereas selective D1-MSNs, increased induced reward. BLA-AC-NAc pathway is crucial for encoding negative valence, demonstrating distinct intra- circuits physiology.

Язык: Английский

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Neuronal diversity and stereotypy at multiple scales through whole brain morphometry

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Ноя. 26, 2024

We conducted a large-scale whole-brain morphometry study by analyzing 3.7 peta-voxels of mouse brain images at the single-cell resolution, producing one largest multi-morphometry databases mammalian brains to date. registered 204 three major imaging modalities Allen Common Coordinate Framework (CCF) atlas, annotated 182,497 neuronal cell bodies, modeled 15,441 dendritic microenvironments, characterized full morphology 1876 neurons along with their axonal motifs, and detected 2.63 million varicosities that indicate potential synaptic sites. Our analyzed six levels information related populations, morphology, arborization, varicosities, sub-neuronal structural quantification diversity stereotypy patterns each level. This integrative provides key anatomical descriptions types across multiple scales features, contributing substantial resource for understanding in brains.

Язык: Английский

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Activation of hypothalamic-pontine-spinal pathway promotes locomotor initiation and functional recovery after spinal cord injury in mice

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

The hypothalamus is critical for regulating behaviors essential survival and locomotion, but how it integrates internal needs transmits locomotion commands to the spinal cord (SC) remains unclear. We found that glutamatergic neurons in lateral hypothalamic area (LHA) are motivated locomotor activity. Using single-neuron projectome analysis, trans-synaptic tracing, optogenetic manipulation, we showed LHA facilitates during food seeking via pontine oral part (PnO) projection neurons, rather than direct SC projections or indirect stress signaling medial septum diagonal band. Activating PnO-SC also initiated locomotion. Importantly, LHA-PnO were crucial recovery following mouse injury (SCI). Motor cortex signals gated deep brain stimulation treatment markedly promoted long-term restoration of hindlimb motor functions after severe SCI. Thus, have identified a hypothalamic-pontine-spinal pathway paradigm potential therapeutic intervention

Язык: Английский

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