Frontiers for Young Minds,
Год журнала:
2023,
Номер
11
Опубликована: Окт. 12, 2023
Salmonella
enterica
is
a
type
of
bacteria
that
can
cause
vomiting
and
diarrhea
in
humans.
Normally,
dies
when
it
on
the
surface
healthy
plant
leaves.
However,
has
“friends”
plants
help
survive.
Plant-eating
insects
phytobacteria
diseases
reach
new
places
access
food
sources.
move
from
one
to
another
provide
with
valuable
nutrients
through
their
poop.
Phytobacteria
sicken
leaves,
creating
an
environment
helps
enter
leaf
protection.
Both
plant-eating
leaves
release
nourish
dangerous
like
.
Together,
combination
or
crops
may
create
perfect
recipe
for
contaminated
produce
leads
disease
humans
they
eat
fruit
veggies.
Food Microbiology,
Год журнала:
2023,
Номер
117, С. 104389 - 104389
Опубликована: Сен. 21, 2023
Ready-to-eat
fruit
and
vegetables
are
a
convenient
source
of
nutrients
fibre
for
consumers,
generally
safe
to
eat,
but
vulnerable
contamination
with
human
enteric
bacterial
pathogens.
Over
the
last
decade,
Salmonella
spp.,
pathogenic
Escherichia
coli,
Listeria
monocytogenes
have
been
linked
most
outbreaks
foodborne
illness
associated
fresh
produce.
The
origins
these
traced
multiple
sources
from
pre-harvest
(soil,
seeds,
irrigation
water,
domestic
wild
animal
faecal
matter)
or
post-harvest
operations
(storage,
preparation
packaging).
These
pathogens
developed
processes
successful
attachment,
survival
colonization
conferring
them
ability
adapt
environments.
However,
differ
across
strains
same
species,
different
plant
species
cultivars.
In
competitive
environment,
additional
risk
factors
microbiome
phyllosphere
responses;
both
directly
modulate
on
leaf's
surface.
Understanding
mechanisms
involved
in
attachment
to,
of,
proliferation,
produce
role
resisting
is
therefore
crucial
reducing
future
outbreaks.
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 3, 2025
Bacterial
disease
alters
the
infection
court
creating
new
niches.
The
apoplast
is
an
oasis
from
hardships
of
leaf
surface
and
generally
inaccessible
to
nonpathogenic
members
phyllosphere
bacterial
community.
Previously,
we
demonstrated
that
Salmonella
enterica
serovar
Typhimurium
(
S.
Typhimurium)
immigrants
can
both
enter
replicate
due
conditions
created
by
established
Xanthomonas
hortorum
pv.
gardneri
(Xhg)
in
tomato.
Here,
have
expanded
our
investigation
how
changes
host
examining
effects
another
water-soaking
pathogen,
Pseudomonas
syringae
tomato
(Pst),
on
immigrating
bacteria.
We
discovered
that,
despite
causing
macroscopically
similar
symptoms
as
Xhg,
Pst
disrupts
colonization
apoplast.
To
determine
if
these
were
broadly
applicable
bacteria,
examined
fates
immigrant
Xhg
arriving
infected
leaf.
found
this
effect
not
specific
Typhimurium,
but
or
also
struggled
fully
join
infecting
population
identify
mechanisms
underlying
results,
quantified
macroscopic
symptoms,
stomata
a
pinch
point
entry,
characterized
aspects
interbacterial
competition.
While
it
may
be
considered
common
knowledge
hosts
are
fundamentally
altered
following
infection,
drive
remain
poorly
understood.
investigated
pathogens
reach
deeper
understanding
rarely
accessible,
inhabitable
environment
obtainable,
habitable
niche.
Molecular Plant Pathology,
Год журнала:
2023,
Номер
25(1)
Опубликована: Окт. 17, 2023
Abstract
Plant‐pathogenic
Ralstonia
strains
cause
bacterial
wilt
disease
by
colonizing
xylem
vessels
of
many
crops,
including
tomato.
Host
resistance
is
the
best
control
for
wilt,
but
mechanisms
widely
used
Hawaii
7996
tomato
breeding
line
(H7996)
are
unknown.
Using
growth
in
ex
vivo
sap
as
a
proxy
host
xylem,
we
found
that
strain
GMI1000
grows
from
both
healthy
plants
and
‐infected
susceptible
plants.
However,
H7996
inhibited
growth,
suggesting
response
to
infection,
resistant
increase
inhibitors
their
sap.
Consistent
with
this,
reciprocal
grafting
defence
gene
expression
experiments
indicated
acts
above‐
belowground
plant
parts.
Concerningly,
broken
UW551
pandemic
lineage
threatens
highland
tropical
agriculture.
Unlike
other
,
grew
well
Moreover,
could
grow
previously
infected
UW551.
Thus,
overcomes
part
detoxifying
Testing
panel
compounds
identified
metabolomics
revealed
no
single
chemical
differentially
inhibits
cannot
infect
H7996.
contained
more
phenolic
compounds,
which
known
be
involved
antimicrobial
defence.
Culturing
this
reduced
total
levels,
indicating
resistance‐breaking
degrades
these
defences.
Together,
results
suggest
depends
on
inducible
Microbiology Spectrum,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 22, 2024
Bacteria
can
coordinate
behavior
in
response
to
population
density
through
the
production,
release,
and
detection
of
small
molecules,
a
phenomenon
known
as
quorum
sensing.
Frontiers in Microbiology,
Год журнала:
2023,
Номер
14
Опубликована: Сен. 7, 2023
Salmonella
enterica,
a
foodborne
and
human
pathogen,
is
constant
threat
to
health.
Agricultural
environments,
for
example,
soil
plants,
can
be
ecological
niches
vectors
transmission.
persistence
in
such
environments
increases
the
risk
consumers.
Therefore,
it
necessary
investigate
mechanisms
used
by
adapt
agricultural
environments.
We
assessed
adaptation
strategy
of
S.
enterica
serovar
Typhimurium
strain
14028s
agricultural-relevant
situations
analyzing
abundance
intermediates
glycolysis
tricarboxylic
acid
pathway
tested
(diluvial
sand
suspension
leaf-based
media
from
tomato
lettuce),
as
well
bacterial
cells
grown
conditions.
By
reanalyzing
transcriptome
data
those
using
an
independent
RT-qPCR
approach
verification,
several
genes
were
identified
important
root
or
leaf
tissues,
including
pyruvate
dehydrogenase
subunit
E1
encoding
gene
aceE.
In
vivo
assay
leaves
confirmed
crucial
role
A
mutant
another
persistence-related
gene,
aceB,
malate
synthase
A,
displayed
opposite
features.
comparing
metabolites
expression
wild-type
its
aceB
mutant,
fumarate
accumulation
was
discovered
potential
way
replenish
effects
mutation.
Our
research
interprets
mechanism
agriculture
adapting
carbon
metabolism
sources
available
environment.
These
insights
may
assist
development
strategies
aimed
at
diminishing
food
production
systems.
Phytobiomes Journal,
Год журнала:
2023,
Номер
7(2), С. 151 - 159
Опубликована: Июнь 15, 2023
This
Meeting
Review
article
offers
a
synthesis
of
the
science
presented
and
discussed
at
recently
held
11th
International
Symposium
on
Leaf
Surface
Microbiology,
also
known
as
Phyllosphere
2022,
University
California–Davis
(UC
Davis),
17
to
21
July
2022.
Twice
postponed
due
Covid-19,
this
in-person
conference
covered
wide-ranging
but
intersecting
topics
related
microbiology
leaves
(and
other
aboveground
parts
plants),
including
phytopathology,
food
safety,
plant
physiology,
microbial
ecology,
fluid
physics,
vegetation
science,
single-cell
biology,
canopy
architecture,
human
health.
The
overarching
theme
meeting
(“understanding
rules
phyllospheric
life”)
was
explored
in
46
podium
12
poster
presentations.
It
fueled
formal
informal
discussions
among
90-plus
participants
about
existing
new
questions
phyllosphere
microbiology.
Are
there
first
principles
underlying
acquisition,
assembly
succession
communities
phyllosphere?
How
best
define,
recognize,
exploit
fitness
microscopic
leaf
dwellers?
At
what
scales
do
or
should
we
sample,
interrogate,
understand
What
still
needs
be
learned
that
keeps
us
from
insights,
resources,
tools
produce
healthier
more
nutritious
foliage?
Having
UC
Davis
summer
unique
opportunity
“vertically
integrate”
high
school
students
COSMOS
program
into
2022
allow
face-to-face
interactions
with
early-career
scientists
prominent
senior
career
professionals
Students
thus
experienced
first-hand
ways
which
address
problems
affect
society
seek
understanding
solutions
those
problems.
represented
an
effective
approach
engage
younger
generation
thinking
research
stewardship
plants
their
foliage
and,
generally,
merits
career.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 30, 2024
ABSTRACT
Bacterial
disease
alters
the
infection
court
creating
new
niches.
The
apoplast
is
an
oasis
from
hardships
of
leaf
surface
and
generally
inaccessible
to
nonpathogenic
members
phyllosphere
bacterial
community.
Previously,
we
demonstrated
that
Salmonella
enterica
immigrants
can
both
enter
replicate
due
conditions
created
by
established
Xanthomonas
hortorum
pv.
gardneri
(Xhg)
infection.
Here,
have
expanded
our
investigation
how
changes
host
examining
effects
another
water-soaking
pathogen,
Pseudomonas
syringae
pv
tomato
(Pst),
on
immigrating
bacteria.
We
discovered
that,
despite
causing
macroscopically
similar
symptoms
as
Xhg,
Pst
disrupts
S.
colonization
apoplast.
To
determine
if
these
were
broadly
applicable
bacteria,
examined
fates
immigrant
Xhg
arriving
infected
leaf.
found
this
effect
not
specific
,
but
or
also
struggled
fully
join
infecting
population
in
identify
mechanisms
underlying
results,
quantified
macroscopic
symptoms,
stomata
a
pinch
point
entry,
characterized
aspects
interbacterial
competition.
While
it
may
be
considered
common
knowledge
hosts
are
fundamentally
altered
following
infection,
drive
remain
poorly
understood.
investigated
pathogens
reach
deeper
understanding
rarely
accessible,
inhabitable
environment
obtainable,
habitable
niche.
IMPORTANCE
Pathogens
dramatically
alter
during
Changes
physical
biochemical
characteristics
benefit
pathogen
reshape
composition
In
fact,
rare
plant
microbiota,
namely
human
pathogens,
such
enterica,
thrive
some
courts.
increased
success
results
conversion
niche
compared
two
phytopathogens,
within
uncovered
relevant
potentially
overlooked
similarity
symptoms.
used
pathogen’s
either
failure
newly
This
study
reveals
information
about
leaves
key
remodel
inhospitable
niches
new,
conducive
environments
diseased
host.