From field to plate: How do bacterial enteric pathogens interact with ready-to-eat fruit and vegetables, causing disease outbreaks?

Food Microbiology, Год журнала: 2023, Номер 117, С. 104389 - 104389

Опубликована: Сен. 21, 2023

Ready-to-eat fruit and vegetables are a convenient source of nutrients fibre for consumers, generally safe to eat, but vulnerable contamination with human enteric bacterial pathogens. Over the last decade, Salmonella spp., pathogenic Escherichia coli, Listeria monocytogenes have been linked most outbreaks foodborne illness associated fresh produce. The origins these traced multiple sources from pre-harvest (soil, seeds, irrigation water, domestic wild animal faecal matter) or post-harvest operations (storage, preparation packaging). These pathogens developed processes successful attachment, survival colonization conferring them ability adapt environments. However, differ across strains same species, different plant species cultivars. In competitive environment, additional risk factors microbiome phyllosphere responses; both directly modulate on leaf's surface. Understanding mechanisms involved in attachment to, of, proliferation, produce role resisting is therefore crucial reducing future outbreaks.

Язык: Английский

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Established Pseudomonas syringae pv. tomato infection disrupts immigration of leaf surface bacteria to the apoplast

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Фев. 3, 2025

Bacterial disease alters the infection court creating new niches. The apoplast is an oasis from hardships of leaf surface and generally inaccessible to nonpathogenic members phyllosphere bacterial community. Previously, we demonstrated that Salmonella enterica serovar Typhimurium ( S. Typhimurium) immigrants can both enter replicate due conditions created by established Xanthomonas hortorum pv. gardneri (Xhg) in tomato. Here, have expanded our investigation how changes host examining effects another water-soaking pathogen, Pseudomonas syringae tomato (Pst), on immigrating bacteria. We discovered that, despite causing macroscopically similar symptoms as Xhg, Pst disrupts colonization apoplast. To determine if these were broadly applicable bacteria, examined fates immigrant Xhg arriving infected leaf. found this effect not specific Typhimurium, but or also struggled fully join infecting population identify mechanisms underlying results, quantified macroscopic symptoms, stomata a pinch point entry, characterized aspects interbacterial competition. While it may be considered common knowledge hosts are fundamentally altered following infection, drive remain poorly understood. investigated pathogens reach deeper understanding rarely accessible, inhabitable environment obtainable, habitable niche.

Язык: Английский

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Time of arrival during plant disease progression and humidity additively influence Salmonella enterica colonization of lettuce

Applied and Environmental Microbiology, Год журнала: 2024, Номер 90(9)

Опубликована: Авг. 29, 2024

The interplay between plant hosts, phytopathogenic bacteria, and enteric human pathogens in the phyllosphere has consequences for health.

Язык: Английский

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Lettuce immune responses and apoplastic metabolite profile contribute to reduced internal leaf colonization by human bacterial pathogens

BMC Plant Biology, Год журнала: 2025, Номер 25(1)

Опубликована: Май 14, 2025

Язык: Английский

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Ralstonia solanacearum pandemic lineage strain UW551 overcomes inhibitory xylem chemistry to break tomato bacterial wilt resistance

Molecular Plant Pathology, Год журнала: 2023, Номер 25(1)

Опубликована: Окт. 17, 2023

Abstract Plant‐pathogenic Ralstonia strains cause bacterial wilt disease by colonizing xylem vessels of many crops, including tomato. Host resistance is the best control for wilt, but mechanisms widely used Hawaii 7996 tomato breeding line (H7996) are unknown. Using growth in ex vivo sap as a proxy host xylem, we found that strain GMI1000 grows from both healthy plants and ‐infected susceptible plants. However, H7996 inhibited growth, suggesting response to infection, resistant increase inhibitors their sap. Consistent with this, reciprocal grafting defence gene expression experiments indicated acts above‐ belowground plant parts. Concerningly, broken UW551 pandemic lineage threatens highland tropical agriculture. Unlike other , grew well Moreover, could grow previously infected UW551. Thus, overcomes part detoxifying Testing panel compounds identified metabolomics revealed no single chemical differentially inhibits cannot infect H7996. contained more phenolic compounds, which known be involved antimicrobial defence. Culturing this reduced total levels, indicating resistance‐breaking degrades these defences. Together, results suggest depends on inducible

Язык: Английский

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Identification of new SdiA regulon members of Escherichia coli , Enterobacter cloacae , and Salmonella enterica serovars Typhimurium and Typhi

Microbiology Spectrum, Год журнала: 2024, Номер unknown

Опубликована: Окт. 22, 2024

Bacteria can coordinate behavior in response to population density through the production, release, and detection of small molecules, a phenomenon known as quorum sensing.

Язык: Английский

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Salmonella enterica relies on carbon metabolism to adapt to agricultural environments

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Сен. 7, 2023

Salmonella enterica, a foodborne and human pathogen, is constant threat to health. Agricultural environments, for example, soil plants, can be ecological niches vectors transmission. persistence in such environments increases the risk consumers. Therefore, it necessary investigate mechanisms used by adapt agricultural environments. We assessed adaptation strategy of S. enterica serovar Typhimurium strain 14028s agricultural-relevant situations analyzing abundance intermediates glycolysis tricarboxylic acid pathway tested (diluvial sand suspension leaf-based media from tomato lettuce), as well bacterial cells grown conditions. By reanalyzing transcriptome data those using an independent RT-qPCR approach verification, several genes were identified important root or leaf tissues, including pyruvate dehydrogenase subunit E1 encoding gene aceE. In vivo assay leaves confirmed crucial role A mutant another persistence-related gene, aceB, malate synthase A, displayed opposite features. comparing metabolites expression wild-type its aceB mutant, fumarate accumulation was discovered potential way replenish effects mutation. Our research interprets mechanism agriculture adapting carbon metabolism sources available environment. These insights may assist development strategies aimed at diminishing food production systems.

Язык: Английский

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Phyllosphere 2022: 11th International Symposium on Leaf Surface Microbiology

Phytobiomes Journal, Год журнала: 2023, Номер 7(2), С. 151 - 159

Опубликована: Июнь 15, 2023

This Meeting Review article offers a synthesis of the science presented and discussed at recently held 11th International Symposium on Leaf Surface Microbiology, also known as Phyllosphere 2022, University California–Davis (UC Davis), 17 to 21 July 2022. Twice postponed due Covid-19, this in-person conference covered wide-ranging but intersecting topics related microbiology leaves (and other aboveground parts plants), including phytopathology, food safety, plant physiology, microbial ecology, fluid physics, vegetation science, single-cell biology, canopy architecture, human health. The overarching theme meeting (“understanding rules phyllospheric life”) was explored in 46 podium 12 poster presentations. It fueled formal informal discussions among 90-plus participants about existing new questions phyllosphere microbiology. Are there first principles underlying acquisition, assembly succession communities phyllosphere? How best define, recognize, exploit fitness microscopic leaf dwellers? At what scales do or should we sample, interrogate, understand What still needs be learned that keeps us from insights, resources, tools produce healthier more nutritious foliage? Having UC Davis summer unique opportunity “vertically integrate” high school students COSMOS program into 2022 allow face-to-face interactions with early-career scientists prominent senior career professionals Students thus experienced first-hand ways which address problems affect society seek understanding solutions those problems. represented an effective approach engage younger generation thinking research stewardship plants their foliage and, generally, merits career.

Язык: Английский

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EstablishedPseudomonas syringaepv. tomato infection disrupts immigration of leaf surface bacteria to the apoplast

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 30, 2024

ABSTRACT Bacterial disease alters the infection court creating new niches. The apoplast is an oasis from hardships of leaf surface and generally inaccessible to nonpathogenic members phyllosphere bacterial community. Previously, we demonstrated that Salmonella enterica immigrants can both enter replicate due conditions created by established Xanthomonas hortorum pv. gardneri (Xhg) infection. Here, have expanded our investigation how changes host examining effects another water-soaking pathogen, Pseudomonas syringae pv tomato (Pst), on immigrating bacteria. We discovered that, despite causing macroscopically similar symptoms as Xhg, Pst disrupts S. colonization apoplast. To determine if these were broadly applicable bacteria, examined fates immigrant Xhg arriving infected leaf. found this effect not specific , but or also struggled fully join infecting population in identify mechanisms underlying results, quantified macroscopic symptoms, stomata a pinch point entry, characterized aspects interbacterial competition. While it may be considered common knowledge hosts are fundamentally altered following infection, drive remain poorly understood. investigated pathogens reach deeper understanding rarely accessible, inhabitable environment obtainable, habitable niche. IMPORTANCE Pathogens dramatically alter during Changes physical biochemical characteristics benefit pathogen reshape composition In fact, rare plant microbiota, namely human pathogens, such enterica, thrive some courts. increased success results conversion niche compared two phytopathogens, within uncovered relevant potentially overlooked similarity symptoms. used pathogen’s either failure newly This study reveals information about leaves key remodel inhospitable niches new, conducive environments diseased host.

Язык: Английский

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Exploring the potential of plant astrobiology: adapting flora for extra-terrestrial habitats: a review

Biologia Futura, Год журнала: 2024, Номер unknown

Опубликована: Сен. 20, 2024

Язык: Английский

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