Journal of Clinical Investigation,
Год журнала:
2024,
Номер
134(8)
Опубликована: Фев. 29, 2024
Staphylococcus
aureus
is
a
leading
cause
of
biofilm-associated
prosthetic
joint
infection
(PJI).
A
primary
contributor
to
chronicity
an
expansion
granulocytic
myeloid-derived
suppressor
cells
(G-MDSCs)
that
are
critical
for
orchestrating
the
anti-inflammatory
biofilm
milieu.
Single-cell
sequencing
and
bioinformatic
metabolic
algorithms
were
used
explore
link
between
G-MDSC
metabolism
S.
PJI
outcome.
Glycolysis
hypoxia
response
through
hypoxia-inducible
factor-1
alpha
(HIF-1a)
significantly
enriched
in
G-MDSCs.
Interfering
with
both
pathways
vivo,
using
2-deoxyglucose
nanopreparation
granulocyte-targeted
HIF-1a
conditional
knockout
mice,
respectively,
attenuated
G-MDSC-mediated
immunosuppression
reduced
bacterial
burden
mouse
model
PJI.
In
addition,
scRNA-seq
analysis
granulocytes
from
patients
also
showed
enrichment
glycolysis
genes.
These
findings
support
importance
glycolysis/HIF-1a
axis
promoting
activity
persistence
during
Biochemical Journal,
Год журнала:
2024,
Номер
481(4), С. 245 - 263
Опубликована: Фев. 15, 2024
Numerous
bacteria
naturally
occur
within
spatially
organised,
multicellular
communities
called
biofilms.
Moreover,
most
bacterial
infections
proceed
with
biofilm
formation,
posing
major
challenges
to
human
health.
Within
biofilms,
cells
are
embedded
in
a
primarily
self-produced
extracellular
matrix,
which
is
defining
feature
of
all
The
matrix
complex,
viscous
mixture
composed
polymeric
substances
such
as
polysaccharides,
filamentous
protein
fibres,
and
DNA.
structured
arrangement
the
bestows
beneficial
emergent
properties
that
not
displayed
by
planktonic
cells,
conferring
protection
against
physical
chemical
stresses,
including
antibiotic
treatment.
However,
lack
multi-scale
information
at
molecular
level
has
prevented
better
understanding
this
its
properties.
Here,
we
review
recent
progress
on
characterisation
components
their
three-dimensional
spatial
organisation
As
the
global
population
ages,
bone
diseases
have
become
increasingly
prevalent
in
clinical
settings.
These
conditions
often
involve
detrimental
factors
such
as
infection,
inflammation,
and
oxidative
stress
that
disrupt
homeostasis.
Addressing
these
disorders
requires
exogenous
strategies
to
regulate
osteogenic
microenvironment
(OME).
The
regulation
of
OME
can
be
divided
into
four
processes:
induction,
modulation,
protection,
support,
each
serving
a
specific
purpose.
To
this
end,
metal-organic
frameworks
(MOFs)
are
an
emerging
focus
nanomedicine,
which
show
tremendous
potential
due
their
superior
delivery
capability.
MOFs
play
numerous
roles
metal
ion
donors,
drug
carriers,
nanozymes,
photosensitizers,
been
extensively
explored
recent
studies.
This
review
presents
comprehensive
introduction
by
MOF-based
nanomaterials.
By
discussing
various
functional
MOF
composites,
work
aims
inspire
guide
creation
sophisticated
efficient
nanomaterials
for
disease
management.
Journal of Clinical Investigation,
Год журнала:
2024,
Номер
134(8)
Опубликована: Фев. 29, 2024
Staphylococcus
aureus
is
a
leading
cause
of
biofilm-associated
prosthetic
joint
infection
(PJI).
A
primary
contributor
to
chronicity
an
expansion
granulocytic
myeloid-derived
suppressor
cells
(G-MDSCs)
that
are
critical
for
orchestrating
the
anti-inflammatory
biofilm
milieu.
Single-cell
sequencing
and
bioinformatic
metabolic
algorithms
were
used
explore
link
between
G-MDSC
metabolism
S.
PJI
outcome.
Glycolysis
hypoxia
response
through
hypoxia-inducible
factor-1
alpha
(HIF-1a)
significantly
enriched
in
G-MDSCs.
Interfering
with
both
pathways
vivo,
using
2-deoxyglucose
nanopreparation
granulocyte-targeted
HIF-1a
conditional
knockout
mice,
respectively,
attenuated
G-MDSC-mediated
immunosuppression
reduced
bacterial
burden
mouse
model
PJI.
In
addition,
scRNA-seq
analysis
granulocytes
from
patients
also
showed
enrichment
glycolysis
genes.
These
findings
support
importance
glycolysis/HIF-1a
axis
promoting
activity
persistence
during
