Biotechnological potential of salt tolerant and xerophilic species of Aspergillus DOI Creative Commons
István Pócsi, Jan Dijksterhuis, Jos Houbraken

и другие.

Applied Microbiology and Biotechnology, Год журнала: 2024, Номер 108(1)

Опубликована: Ноя. 19, 2024

Abstract Xerophilic fungi occupy versatile environments owing to their rich arsenal helping them successfully adapt water constraints as a result of low relative humidity, high-osmolarity, and high-salinity conditions. The general term xerophilic relates organisms that tolerate and/or require reduced activity, while halophilic osmophilic are applied specialized groups high salt concentrations or increased osmotic pressure, respectively. Species belonging the family Aspergillaceae , especially those classified in Aspergillus subgenus (sections Restricti ) Polypaecilum particularly enriched group salt-tolerant filamentous fungi. They produce an unprecedently wide spectrum tolerant enzymes including proteases, peptidases, glutaminases, γ-glutamyl transpeptidases, various glycosidases such cellulose-decomposing starch-degrading hydrolases, lipases, tannases, oxidareductases. These extremophilic also represent huge untapped treasure chest yet-to-be-discovered, highly valuable, biologically active secondary metabolites. Furthermore, these indispensable agents decolorizing textile dyes, degrading xenobiotics removing excess ions high-salt environments. could play role fermentation processes at activity leading preparation daqu, meju, tea. Considering current future agricultural applications, Aspergilli may contribute biosolubilization phosphate soil amelioration stress crops. Transgenes from halophile find promising applications engineering drought-tolerant spoil feed food raise mycotoxin above permissible doses and, therefore, development novel preservation technologies against spp. is urgently needed. On other hand, some have been shown be biological control mites. Key points • Salt can found resources valuable metabolites Biotechnological expanding

Язык: Английский

OMICS and Other Advanced Technologies in Mycological Applications DOI Creative Commons
Nalin N. Wijayawardene, Nattawut Boonyuen, C. B. Ranaweera

и другие.

Journal of Fungi, Год журнала: 2023, Номер 9(6), С. 688 - 688

Опубликована: Июнь 19, 2023

Fungi play many roles in different ecosystems. The precise identification of fungi is important aspects. Historically, they were identified based on morphological characteristics, but technological advancements such as polymerase chain reaction (PCR) and DNA sequencing now enable more accurate taxonomy, higher-level classifications. However, some species, referred to "dark taxa", lack distinct physical features that makes their challenging. High-throughput metagenomics environmental samples provide a solution identifying new lineages fungi. This paper discusses approaches including PCR amplification rDNA, multi-loci phylogenetic analyses, the importance various omics (large-scale molecular) techniques for understanding fungal applications. use proteomics, transcriptomics, metatranscriptomics, metabolomics, interactomics provides comprehensive These advanced technologies are critical expanding knowledge Kingdom Fungi, its impact food safety security, edible mushrooms foodomics, secondary metabolites, mycotoxin-producing fungi, biomedical therapeutic applications, antifungal drugs drug resistance, data novel development. also highlights exploring from extreme environments understudied areas identify dark taxa.

Язык: Английский

Процитировано

32

Statistical optimization of pectinases from thermophilic Aspergillus fumigatus BT-4 employing response surface methodology through submerged fermentation using agricultural wastes DOI Creative Commons
Imran Ali, Roheena Abdullah,

Sana Ullah Saqib

и другие.

BMC Biotechnology, Год журнала: 2025, Номер 25(1)

Опубликована: Янв. 4, 2025

In this study, thermophilic pectinase-producing strains were isolated. Among all the isolates, strain No. 4 was identified as Aspergillus fumigatus BT-4 based on its morphology and 18 S rDNA analysis. This employed to screen various fermentation media enhance pectinase production. Pectinases are crucial enzymes with significant industrial applications, particularly in food textile industries. Identifying efficient producers optimizing their production processes essential for improving applications. Maximum observed using 1% grapefruit peel M5 media. Shake flask kinetics demonstrated highest values of specific rate constant (qp), growth (µ), product yield coefficient (Yp/x), volumetric formation (Qp), biomass (Qx) after 72 h incubation. Furthermore, Optimization components via Response Surface Methodology (RSM) improved by 50%, showcasing effectiveness factorial central composite designs fine-tuning parameters. The use agricultural waste (grapefruit peel) significantly reduced costs, offering an economically viable substrate alternative. enzyme purified through ammonium sulfate precipitation, gel filtration, ion-exchange chromatography, resulting a 2.3-fold purification. molecular weight determined be 48 kDa. Enzyme kinetics, Lineweaver-Burk plot at pectin concentrations, showed Vmax 32.7 UmL− 1 Km 0.3 mg mL− 1. Thermodynamic parameters, including activation energy (Ea), enthalpy (ΔH), entropy (ΔS), measured 41.74 kJmol− 1, 39.53 46.9 respectively. study successfully isolated potent producer. process enhanced Using reduces costs eliminating need expensive raw materials utilizing low-cost, sustainable, locally available substrate. approach also minimizes disposal expenses, making more economical. effectively purified, kinetic thermodynamic properties thoroughly characterized, revealing potential comprehensive analysis optimization strategies provides robust foundation scaling up production, contributing cost-effective processes.

Язык: Английский

Процитировано

2

Reducing the number of accepted species inAspergillusseriesNigri DOI Creative Commons
Chao Bian, Yoko Kusuya, F. Sklenář

и другие.

Studies in Mycology, Год журнала: 2022, Номер 102(1), С. 95 - 132

Опубликована: Июнь 30, 2022

Язык: Английский

Процитировано

30

Know the enemy and know yourself: Addressing cryptic fungal pathogens of humans and beyond DOI Creative Commons
Jacob L. Steenwyk, Antonis Rokas, Gustavo H. Goldman

и другие.

PLoS Pathogens, Год журнала: 2023, Номер 19(10), С. e1011704 - e1011704

Опубликована: Окт. 19, 2023

If you know the enemy and yourself, need not fear result of a hundred battles.If yourself but enemy, for every victory gained will also suffer defeat.If neither nor succumb in battle."-SunTzu, The Art War Fungal pathogens threaten human welfare, including animal health global food security.For example, ameliorating crop loss due to fungal plant would feed nearly 600 million more people [1].Fungal infections humans are difficult diagnose treat, potentially leading serious illness death, especially immunocompromised individuals; case point, there an estimated 1.7 deaths per year than 150 severe worldwide [2].These issues may be exacerbated climate change.Reflecting importance combatting preventing infection diseases caused by fungi, World Health Organization recently released list priority (https://www.who.int/publications/i/item/9789240060241).Accordingly, fungi that cause disease have become major research focus, resulting deeper, still incomplete, understanding virulence cadre pathogenic species.Most opportunistic affect individuals.This suggests any fungus capable growing body overcoming or evading compromised immune system could disease.Supporting this notion, advances DNA sequencing clinical isolates led realization cryptic speciesmorphologically indistinguishable from known genetically distinct-are underappreciated source [3][4][5][6].Cryptic species been identified several genera agricultural biomedical significance, such as (e.g., Fusarium) [7] Aspergillus; Table 1) [3].Due morphological similarities (Fig 1 ), using typical microbiology techniques setting, incomplete their epidemiology burden.Here, avenues aim enrich our pathogens-as well other pathogens-and diminish impact on welfare discussed.Cryptic genus Aspergillus used example.We argue infections, among which little is known, can reduced if we in-depth pathogens, humans, interaction between two.

Язык: Английский

Процитировано

12

Multi-omics insights into the response of Aspergillus parasiticus to long-chain alkanes in relation to polyethylene modification DOI Creative Commons
Romanos Siaperas, George Taxeidis, Αnastasia Gioti

и другие.

Environmental Pollution, Год журнала: 2025, Номер 376, С. 126386 - 126386

Опубликована: Май 8, 2025

Язык: Английский

Процитировано

0

Additions to Pleosporalean Taxa Associated with Xanthoceras sorbifolium from Jilin and Hebei, China DOI Creative Commons
Rong Xu, Yu Li

Microorganisms, Год журнала: 2025, Номер 13(6), С. 1296 - 1296

Опубликована: Май 31, 2025

Pleosporalean fungi play significant roles as plant pathogens, saprobes, and endophytes in a wide variety of economically important hosts. During an investigation saprobic from Jilin Hebei, China, five pleosporalean isolates were obtained the dead stems Xanthoceras sorbifolium. Morphological evidence multi-locus sequence analyses using combined dataset ITS, LSU, SSU, rpb2, tef1-α, tub2 indicate that these represent two new species (Alloleptosphaeria xanthoceratis Lophiostoma multiforme) record montanae. Full morphological descriptions illustrations are provided herein, phylogenetic relationships three taxa also discussed. Detailed presented, along with affiliations taxa.

Язык: Английский

Процитировано

0

PhyKIT: A Multitool for Phylogenomics DOI Creative Commons
Jacob L. Steenwyk, Gemma I. Martínez‐Redondo, Thomas J. Buida

и другие.

Current Protocols, Год журнала: 2024, Номер 4(11)

Опубликована: Окт. 30, 2024

Abstract Multiple sequence alignments and phylogenetic trees are rich in biological information fundamental to research biology. PhyKIT is a tool for processing analyzing the content of multiple trees. Here, we describe how use diverse analyses, including (i) constructing phylogenomic supermatrix, (ii) detecting errors orthology inference, (iii) quantifying biases data sets, (iv) identifying radiation events or lack resolution using gene support frequencies, (v) conducting evolution‐based screens facilitate function prediction. Several functions that streamline alignment processing—such as renaming FASTA entries tree tips—are also discussed. These protocols demonstrate simple command‐line operations unified framework analysis processing, from supermatrix construction diagnosis gaining clues about function. © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1 : Installing syntax usage 2 Constructing 3 Detecting anomalies relationships 4 Quantifying matrices related measures 5 Identifying polytomies 6 Assessing gene‐gene coevolution genetic screen

Язык: Английский

Процитировано

3

The relevance of genomics in food mycology with a focus on food safety DOI
Jéssica Gil-Serna, Covadonga Vázquez, Belén Patiño

и другие.

Current Opinion in Food Science, Год журнала: 2024, Номер 59, С. 101213 - 101213

Опубликована: Авг. 23, 2024

Язык: Английский

Процитировано

1

Strain heterogeneity in a non-pathogenic Aspergillus fungus highlights factors associated with virulence DOI Creative Commons
David C. Rinker, Thomas J. C. Sauters, Karin Steffen

и другие.

Communications Biology, Год журнала: 2024, Номер 7(1)

Опубликована: Сен. 4, 2024

Язык: Английский

Процитировано

1

Reticulate evolution: Detection and utility in the phylogenomics era DOI Creative Commons

Saelin Bjornson,

Heroen Verbruggen, Nathan S. Upham

и другие.

Molecular Phylogenetics and Evolution, Год журнала: 2024, Номер 201, С. 108197 - 108197

Опубликована: Сен. 11, 2024

Язык: Английский

Процитировано

1