Excessive Ethanol Intake in Mice Does Not Impair Recovery of Torque after Repeated Bouts of Eccentric Contractions

Medicine & Science in Sports & Exercise, Год журнала: 2023, Номер 55(5), С. 873 - 883

Опубликована: Янв. 5, 2023

Alcoholics develop muscle atrophy and weakness from excessive ethanol (EtOH) intake. To date, most research has examined outcomes of alcohol-induced under basal or unstressed conditions despite physical stress being a normal occurrence in physiological setting. Therefore, this study set out to determine if recovery torque is impaired after repetitive bouts skeletal during short-term (experiment 1) long-term 2) EtOH consumption.Twenty male female mice were assigned receive either 20% their drinking water 100% water. Short- consumption was predetermined be intake starting at 4 26 wk, respectively. Anterior crural muscles performed repeated using vivo eccentric contractions, with tetanic isometric measured immediately pre- postinjury. A total 10 completed 14 d between each bout within 1-5 6-10 2), 12 wk 5 6.Mice consuming had blood alcohol concentrations up 270 mg·dL -1 . In experiment 1, five contractions did not reduce torque, regardless sex treatment ( P ≥ 0.173). Similarly, 2, preinjury torques differ day values 0.322). However, there group effect for 6 weaker than controls ≤ 0.002).Excessive short- misuse mouse model affect the muscle's ability regain strength suggesting that may as detrimental once predicted.

Язык: Английский

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Chronic alcohol feeding alters lymph and plasma proteome in a rodent model

Life Sciences, Год журнала: 2023, Номер 327, С. 121818 - 121818

Опубликована: Июнь 1, 2023

Chronic alcohol consumption in rodents induces mesenteric collecting lymphatic vessel hyperpermeability, lymph leakage, and consequent immunometabolic dysregulation of the perilymphatic adipose tissue (PLAT). The specific components mediating PLAT remain to be identified. Specifically, whether impacts composition is unknown. This study aimed determine associated changes plasma proteome. Adult male rats were fed a Lieber–DeCarli liquid diet containing 36 % calories from for 10 weeks. Time-matched control animals pair-fed. At sacrifice was collected 2 h using lymph-fistula technique prior sacrifice. Quantitative discovery-based proteomics identified total 703 proteins. An integrative approach combining Ingenuity Pathway Analysis (IPA) an unbiased network analysis WGCNA (Weighted Gene Co-expression Network Analysis) used analyze data. IPA results significant upregulation cluster apolipoproteins alcohol-fed compared with pair-fed controls downregulation 34 proteins animals. several candidate hub that also significantly differentially expressed controls. module without enrichment Of 59 contained within this module, only Future studies will investigate further functionality affected by feeding both plasma.

Язык: Английский

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Inter- and transgenerational heritability of preconception chronic stress or alcohol exposure: Translational outcomes in brain and behavior

Neurobiology of Stress, Год журнала: 2023, Номер 29, С. 100603 - 100603

Опубликована: Дек. 25, 2023

Chronic stress and alcohol (ethanol) use are highly interrelated can change an individual's behavior through molecular adaptations that do not the DNA sequence, but instead gene expression. A recent wealth of research has found these nongenomic changes be transmitted across generations, which could partially account for "missing heritability" observed in genome-wide association studies disorder other stress-related neuropsychiatric disorders. In this review, we summarize behavioral outcomes inheritance chronic ethanol exposure germline mechanisms give rise to heritability. doing so, outline need further to: (1) Investigate individual paternal, maternal, biparental stress- ethanol-related inheritance; (2) Synthesize dissect cross-generational exposure; (3) Determine preconception contribute alcohol-related disease risk, using cancer as example. detailed understanding effects and/or will yield novel insight into impact ancestral perturbations on risk generations uncover actionable targets improve human health.

Язык: Английский

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Impacto do álcool no organismo: uma revisão dos mecanismos fisiopatológicos envolvidos na toxicidade sistêmica

Brazilian Journal of Health Review, Год журнала: 2024, Номер 7(1), С. 7660 - 7684

Опубликована: Фев. 29, 2024

Introdução: O álcool tem sido consumido desde os primórdios da civilização e se tornou parte integrante de diferentes culturas ao longo do tempo, sendo seu consumo excessivo reconhecido como uma doença na era contemporânea. Apesar disso, o número usuários crescido cada vez mais. alcoolismo representa um desafio significativo saúde pública, que está associado a série desequilíbrios nos sistemas orgânicos. Metodologia: estudo trata-se revisão integrativa literatura acerca dos mecanismos fisiopatológicos envolvem formação danos celulares induzidos pela toxicidade álcool. A estratégia usada para levantamento artigos foi busca ativa por estudos base dados PubMed. Resultados Discussões: Os pelo podem ser divididos em três principais, são direta, modulação processos injúrias via radicais livres, todos podendo causados etanol, bem seus resíduos metabólicos com destaque acetaldeído. Praticamente orgânicos humanos sofrem relacionados bebidas alcoólicas, variando acordo dose, padrão ingestão metabolismo inato indivíduo. Conclusão: efeitos adversos todo corpo, afetando principalmente sistema cardiovascular, digestório, endócrino, nervoso, musculoesquelético, imunológico respiratório, tornando necessária abordagem multidisciplinar entender mitigar esses efeitos, promovendo pública.

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Mesenchymal stem/stromal cells armored by FGF21 ameliorate alcohol-induced liver injury through modulating polarization of macrophages

Hepatology Communications, Год журнала: 2024, Номер 8(4)

Опубликована: Март 29, 2024

Background: Alcohol-associated liver disease (ALD) is a major health care challenge worldwide with limited therapeutic options. Although mesenchymal stem/stromal cells (MSCs) represent newly emerging approach to treat ALD, thus far, there have been extensive efforts try and enhance their efficacy, including genetically engineering MSCs. FGF21, an endocrine stress-responsive hormone, has shown regulate energy balance, glucose, lipid metabolism the homing of MSCs toward injured sites. Therefore, purpose this study was investigate whether that overexpress FGF21 (FGF21-MSCs) improve effect in treating ALD. Methods: Human umbilical cord-derived served as gene delivery vehicle for gene. were transduced using lentiviral vectors mediate overexpression. We utilized both chronic Lieber-DeCarli Gao-binge models ethanol-induced injury observe FGF21-MSCs. Liver phenotypically evaluated by performing biochemical methods, histology, inflammatory cytokine levels. Results: Compared alone, administration overexpressing FGF21(FGF21-MSCs) treatment significantly enhanced ALD mice, indicated alleviation reduced steatosis, infiltration, oxidative stress, hepatic apoptosis, promotion regeneration. Mechanistically, could facilitate immunomodulatory function on macrophages setting metabolic commitment phosphorylation, which enables exhibit anti-inflammatory inclination. Conclusions: Our data elucidate MSC modification may help exploration effective MSCs-based cell therapies

Язык: Английский

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