Advancements in tissue and organ 3D bioprinting: Current techniques, applications, and future perspectives

Materials & Design, Год журнала: 2024, Номер 240, С. 112853 - 112853

Опубликована: Март 19, 2024

3D bioprinting techniques have emerged as a flexible tool in tissue engineering and regenerative medicine to fabricate or pattern functional bio-structures with precise geometric designs, bridging the divergence between engineered natural constructs. A significantly increasing development has been achieved understanding relationship 3D-printing process structures, properties, applications of objects created. The ongoing advancement novel biomaterial inks enabled manufacturing models vitro implants capable achieving some level success preclinical trials. Remarkable progress cell biology biology-inspired computational design assisted latest milestone planned tissue- organ-like constructs having specific levels functionality. However, biofabricated still long way go before reaching clinics. This review presents picture context medicine, focus on biomaterials-related design-centred aspects. Biomedical are described detail relation major tissues organs considered human body. Current technical limitations, challenges, future prospects improvements critically outlined discussed.

Язык: Английский

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Advances in Regenerative Dentistry Approaches: An Update

International Dental Journal, Год журнала: 2023, Номер 74(1), С. 25 - 34

Опубликована: Авг. 2, 2023

Regenerative dentistry is a rapidly evolving field in dentistry, which has been driven by advancements biomedical engineering research and the rising treatment expectations demands that exceed scope of conventional approaches. Tissue engineering, foundation regenerative mainly focuses on 3 key components: stem cells, bioactive molecules, scaffolds. Dental tissue-derived cells are especially significant this regard due to their remarkable properties. techniques have provided novel approaches many strategies various disciplines dentistry. For instance, endodontic procedures such as pulp revascularisation an alternative approach root canal treatment. In addition, surgical nonsurgical periodontal being taken over modified guided tissue regeneration with aid 3-dimensional bioprinting computer-aided design, revolutionised oral maxillofacial engineering. This review presents concise overview latest emerged into clinical practice, potential future technologies, role dental

Язык: Английский

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Injectable decellularized extracellular matrix hydrogel promotes salivary gland regeneration via endogenous stem cell recruitment and suppression of fibrogenesis

Acta Biomaterialia, Год журнала: 2023, Номер 169, С. 256 - 272

Опубликована: Авг. 8, 2023

Язык: Английский

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Progress in salivary glands: Endocrine glands with immune functions

Frontiers in Endocrinology, Год журнала: 2023, Номер 14

Опубликована: Фев. 3, 2023

The production and secretion of saliva is an essential function the salivary glands. Saliva a complicated liquid with different functions, including moistening, digestion, mineralization, lubrication, mucosal protection. This review focuses on mechanism neural regulation secretion, secreted in response to various stimuli, odor, taste, vision, mastication. chemical physical properties change dynamically during physiological pathophysiological processes. Moreover, central nervous system modulates via neurotransmitters neuroreceptors. Smell, taste have been investigated for connection between salivation brain function. immune endocrine functions glands explored recently. Salivary play role innate adaptive immunity Various cells such as B cells, T macrophages, dendritic well immunoglobins like IgA IgG found Evidence supports synthesis corticosterone, testosterone, melatonin contains many potential biomarkers derived from epithelial gingival crevicular fluid, serum. High level matrix metalloproteinases cytokines are markers oral carcinoma, infectious disease cavity, systemic disease. Further research required monitor predict health clinical practice precision medicine.

Язык: Английский

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Xerostomia and Its Cellular Targets

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(6), С. 5358 - 5358

Опубликована: Март 10, 2023

Xerostomia, the subjective feeling of a dry mouth associated with dysfunction salivary glands, is mainly caused by radiation and chemotherapy, various systemic autoimmune diseases, drugs. As saliva plays numerous essential roles in oral health, xerostomia significantly reduces quality life, but its prevalence increasing. Salivation depends on parasympathetic sympathetic nerves, glands responsible for this secretion move fluid unidirectionally through structural features such as polarity acinar cells. Saliva initiated binding released neurotransmitters from nerves to specific G-protein-coupled receptors (GPCRs) This signal induces two intracellular calcium (Ca2+) pathways (Ca2+ release endoplasmic reticulum Ca2+ influx across plasma membrane), increased concentration ([Ca2+]i) causes translocation water channel aquaporin 5 (AQP5) apical membrane. Consequently, GPCR-mediated [Ca2+]i cells promotes secretion, moves into cavity ducts. In review, we seek elucidate potential GPCRs, inositol 1,4,5-trisphosphate receptor (IP3R), store-operated entry (SOCE), AQP5, which are salivation, cellular targets etiology xerostomia.

Язык: Английский

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Bioengineering in salivary gland regeneration

Journal of Biomedical Science, Год журнала: 2022, Номер 29(1)

Опубликована: Июнь 6, 2022

Abstract Salivary gland (SG) dysfunction impairs the life quality of many patients, such as patients with radiation therapy for head and neck cancer Sjögren’s syndrome. Multiple SG engineering strategies have been considered regeneration, repair, or whole organ replacement. An in-depth understanding development differentiation epithelial stem progenitor cells niche during branching morphogenesis signaling pathways involved in cell–cell communication constitute a prerequisite to suitable bioengineering solutions. This review summarizes essential features be fabricate an engineered functional model using various cell types, biomaterials, active agents, matrix fabrication methods. Furthermore, recent innovative promising approaches models are described. Finally, this discusses different challenges future perspectives bioengineering.

Язык: Английский

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Current developments and opportunities of pluripotent stem cells-based therapies for salivary gland hypofunction

Frontiers in Cell and Developmental Biology, Год журнала: 2024, Номер 12

Опубликована: Янв. 19, 2024

Salivary gland hypofunction (SGH) caused by systemic disease, drugs, aging, and radiotherapy for head neck cancer can cause dry mouth, which increases the risk of disorders such as periodontitis, taste disorders, pain burning sensations in dental caries, dramatically reduces quality life patients. To date, treatment SGH is still aimed at relieving patients’ clinical symptoms improving their life, not able to repair regenerate damaged salivary glands. Pluripotent stem cells (PSCs), including embryonic (ESCs), induced pluripotent (iPSCs), extended (EPSCs), are an emerging source cellular therapies that capable unlimited proliferation differentiation into all three germ layers. In recent years, immunomodulatory tissue regenerative effects PSCs, derived cells, paracrine products these have received increasing attention demonstrated promising therapeutic some preclinical studies targeting SGH. This review outlined etiologies available treatments The existing efficacy potential role summarized, with a focus on PSC-derived stem/progenitor (SGS/PCs) mesenchymal (MSCs). this Review, we provide conceptual outline our current understanding PSCs-based therapy its importance treatment, may inform serve design future studies.

Язык: Английский

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High-Resolution Transcriptomic Landscape of the Human Submandibular Gland

Journal of Dental Research, Год журнала: 2023, Номер 102(5), С. 525 - 535

Опубликована: Фев. 1, 2023

Saliva-secreting and transporting cells are part of the complex cellular milieu human salivary gland, where they play important roles in normal glandular physiology diseased states. However, comprehensive molecular characterization, particularly at single-cell resolution, is still incomplete, due to difficulty procuring tissues. Here, we perform an in-depth analysis male female adult submandibular gland (SMG) samples by bulk RNA sequencing (RNA-seq) examine underpinnings heterogeneous cell populations (sc) RNA-seq. Our results from scRNA-seq highlight remarkable diversity clusters epithelial nonepithelial that reside SMG also faithfully recapitulated deconvolution bulk-RNA data sets. analyses reveal transcriptomic heterogeneity within both ductal acinar subpopulations identify atypical types, such as mucoacinar unique humans ionocytes have been recently described mouse. We use CellChat explore ligand–receptor interactome predictions likely mediate crucial cell–cell communications between various clusters. Finally, apply a trajectory inference method investigate specific branching points topology offers insights into dynamic differentiation process SMG. The sets herein comprise extensive wealth high-resolution information valuable resource for deeper mechanistic understanding biology pathophysiology.

Язык: Английский

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Slow hydrogel matrix degradation enhances salivary gland mimetic phenotype

Acta Biomaterialia, Год журнала: 2023, Номер 166, С. 187 - 200

Опубликована: Май 6, 2023

We recently developed a salivary gland tissue mimetic (SGm), comprised of cells encapsulated in matrix metalloproteinase (MMP)-degradable poly(ethylene glycol) hydrogels within arrays ∼320 µm diameter spherical cavities molded PDMS. The SGm provides functional and physiologically relevant platform well-suited to high-throughput drug screening for radioprotective compounds. However, the utility would benefit from improved retention acinar cell phenotype function. hypothesized that tuning biochemical cues presented PEG hydrogel improve maintenance function by mimicking natural extracellular microenvironment intact gland. Hydrogels formed using slower-degrading MMP-sensitive peptide crosslinkers showed >2-fold increase sphere number at 48 h, increased expression markers, more robust response calcium stimulation secretory agonist, carbachol, with reduced cluster disruption outgrowth during prolonged culture. incorporation adhesive peptides containing RGD or IKVAV flux agonists 14 days Tuning aggregation, promoted phenotype, stability over Furthermore, combining this optimized media conditions synergistically SGm. STATEMENT OF SIGNIFICANCE: Salivary (SG) dysfunction occurs due off-target radiation head neck cancer treatments. Progress understanding developing therapeutic strategies SG are hampered lack vitro models, as rapidly lose critical 24 hours vitro. Herein, we identify properties matrices enhance mimetics previously-described SG-microbubble chip environment. Combining slow-degrading marker further enhanced expression.

Язык: Английский

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GZMK+CD8+ T cells Target a Specific Acinar Cell Type in Sjögren’s Disease

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Дек. 19, 2023

Sjögren's Disease (SjD) is a systemic autoimmune disease without clear etiology or effective therapy. Utilizing unbiased single-cell and spatial transcriptomics to analyze human minor salivary glands in health we developed comprehensive understanding of the cellular landscape healthy how that changes SjD patients. We identified novel seromucous acinar cell types population

Язык: Английский

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