Materials & Design, Journal Year: 2024, Volume and Issue: 240, P. 112853 - 112853
Published: March 19, 2024
3D bioprinting techniques have emerged as a flexible tool in tissue engineering and regenerative medicine to fabricate or pattern functional bio-structures with precise geometric designs, bridging the divergence between engineered natural constructs. A significantly increasing development has been achieved understanding relationship 3D-printing process structures, properties, applications of objects created. The ongoing advancement novel biomaterial inks enabled manufacturing models vitro implants capable achieving some level success preclinical trials. Remarkable progress cell biology biology-inspired computational design assisted latest milestone planned tissue- organ-like constructs having specific levels functionality. However, biofabricated still long way go before reaching clinics. This review presents picture context medicine, focus on biomaterials-related design-centred aspects. Biomedical are described detail relation major tissues organs considered human body. Current technical limitations, challenges, future prospects improvements critically outlined discussed.
Language: Английский
Citations
37Advances in Regenerative Dentistry Approaches: An Update
International Dental Journal, Journal Year: 2023, Volume and Issue: 74(1), P. 25 - 34
Published: Aug. 2, 2023
Regenerative dentistry is a rapidly evolving field in dentistry, which has been driven by advancements biomedical engineering research and the rising treatment expectations demands that exceed scope of conventional approaches. Tissue engineering, foundation regenerative mainly focuses on 3 key components: stem cells, bioactive molecules, scaffolds. Dental tissue-derived cells are especially significant this regard due to their remarkable properties. techniques have provided novel approaches many strategies various disciplines dentistry. For instance, endodontic procedures such as pulp revascularisation an alternative approach root canal treatment. In addition, surgical nonsurgical periodontal being taken over modified guided tissue regeneration with aid 3-dimensional bioprinting computer-aided design, revolutionised oral maxillofacial engineering. This review presents concise overview latest emerged into clinical practice, potential future technologies, role dental
Language: Английский
Citations
28Xerostomia and Its Cellular Targets
International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(6), P. 5358 - 5358
Published: March 10, 2023
Xerostomia, the subjective feeling of a dry mouth associated with dysfunction salivary glands, is mainly caused by radiation and chemotherapy, various systemic autoimmune diseases, drugs. As saliva plays numerous essential roles in oral health, xerostomia significantly reduces quality life, but its prevalence increasing. Salivation depends on parasympathetic sympathetic nerves, glands responsible for this secretion move fluid unidirectionally through structural features such as polarity acinar cells. Saliva initiated binding released neurotransmitters from nerves to specific G-protein-coupled receptors (GPCRs) This signal induces two intracellular calcium (Ca2+) pathways (Ca2+ release endoplasmic reticulum Ca2+ influx across plasma membrane), increased concentration ([Ca2+]i) causes translocation water channel aquaporin 5 (AQP5) apical membrane. Consequently, GPCR-mediated [Ca2+]i cells promotes secretion, moves into cavity ducts. In review, we seek elucidate potential GPCRs, inositol 1,4,5-trisphosphate receptor (IP3R), store-operated entry (SOCE), AQP5, which are salivation, cellular targets etiology xerostomia.
Language: Английский
Citations
17Injectable decellularized extracellular matrix hydrogel promotes salivary gland regeneration via endogenous stem cell recruitment and suppression of fibrogenesis
Acta Biomaterialia, Journal Year: 2023, Volume and Issue: 169, P. 256 - 272
Published: Aug. 8, 2023
Language: Английский
Citations
17Current developments and opportunities of pluripotent stem cells-based therapies for salivary gland hypofunction
Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12
Published: Jan. 19, 2024
Salivary gland hypofunction (SGH) caused by systemic disease, drugs, aging, and radiotherapy for head neck cancer can cause dry mouth, which increases the risk of disorders such as periodontitis, taste disorders, pain burning sensations in dental caries, dramatically reduces quality life patients. To date, treatment SGH is still aimed at relieving patients’ clinical symptoms improving their life, not able to repair regenerate damaged salivary glands. Pluripotent stem cells (PSCs), including embryonic (ESCs), induced pluripotent (iPSCs), extended (EPSCs), are an emerging source cellular therapies that capable unlimited proliferation differentiation into all three germ layers. In recent years, immunomodulatory tissue regenerative effects PSCs, derived cells, paracrine products these have received increasing attention demonstrated promising therapeutic some preclinical studies targeting SGH. This review outlined etiologies available treatments The existing efficacy potential role summarized, with a focus on PSC-derived stem/progenitor (SGS/PCs) mesenchymal (MSCs). this Review, we provide conceptual outline our current understanding PSCs-based therapy its importance treatment, may inform serve design future studies.
Language: Английский
Citations
6Bioengineering in salivary gland regeneration
Journal of Biomedical Science, Journal Year: 2022, Volume and Issue: 29(1)
Published: June 6, 2022
Abstract Salivary gland (SG) dysfunction impairs the life quality of many patients, such as patients with radiation therapy for head and neck cancer Sjögren’s syndrome. Multiple SG engineering strategies have been considered regeneration, repair, or whole organ replacement. An in-depth understanding development differentiation epithelial stem progenitor cells niche during branching morphogenesis signaling pathways involved in cell–cell communication constitute a prerequisite to suitable bioengineering solutions. This review summarizes essential features be fabricate an engineered functional model using various cell types, biomaterials, active agents, matrix fabrication methods. Furthermore, recent innovative promising approaches models are described. Finally, this discusses different challenges future perspectives bioengineering.
Language: Английский
Citations
27Progress in salivary glands: Endocrine glands with immune functions
Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14
Published: Feb. 3, 2023
The production and secretion of saliva is an essential function the salivary glands. Saliva a complicated liquid with different functions, including moistening, digestion, mineralization, lubrication, mucosal protection. This review focuses on mechanism neural regulation secretion, secreted in response to various stimuli, odor, taste, vision, mastication. chemical physical properties change dynamically during physiological pathophysiological processes. Moreover, central nervous system modulates via neurotransmitters neuroreceptors. Smell, taste have been investigated for connection between salivation brain function. immune endocrine functions glands explored recently. Salivary play role innate adaptive immunity Various cells such as B cells, T macrophages, dendritic well immunoglobins like IgA IgG found Evidence supports synthesis corticosterone, testosterone, melatonin contains many potential biomarkers derived from epithelial gingival crevicular fluid, serum. High level matrix metalloproteinases cytokines are markers oral carcinoma, infectious disease cavity, systemic disease. Further research required monitor predict health clinical practice precision medicine.
Language: Английский
Citations
15Permanent and decidua dentition as chronological biomarkers of heavy metal contamination: A review of the forensic literature
Journal of Trace Elements in Medicine and Biology, Journal Year: 2024, Volume and Issue: 84, P. 127435 - 127435
Published: March 19, 2024
Language: Английский
Citations
5Slow hydrogel matrix degradation enhances salivary gland mimetic phenotype
Acta Biomaterialia, Journal Year: 2023, Volume and Issue: 166, P. 187 - 200
Published: May 6, 2023
We recently developed a salivary gland tissue mimetic (SGm), comprised of cells encapsulated in matrix metalloproteinase (MMP)-degradable poly(ethylene glycol) hydrogels within arrays ∼320 µm diameter spherical cavities molded PDMS. The SGm provides functional and physiologically relevant platform well-suited to high-throughput drug screening for radioprotective compounds. However, the utility would benefit from improved retention acinar cell phenotype function. hypothesized that tuning biochemical cues presented PEG hydrogel improve maintenance function by mimicking natural extracellular microenvironment intact gland. Hydrogels formed using slower-degrading MMP-sensitive peptide crosslinkers showed >2-fold increase sphere number at 48 h, increased expression markers, more robust response calcium stimulation secretory agonist, carbachol, with reduced cluster disruption outgrowth during prolonged culture. incorporation adhesive peptides containing RGD or IKVAV flux agonists 14 days Tuning aggregation, promoted phenotype, stability over Furthermore, combining this optimized media conditions synergistically SGm. STATEMENT OF SIGNIFICANCE: Salivary (SG) dysfunction occurs due off-target radiation head neck cancer treatments. Progress understanding developing therapeutic strategies SG are hampered lack vitro models, as rapidly lose critical 24 hours vitro. Herein, we identify properties matrices enhance mimetics previously-described SG-microbubble chip environment. Combining slow-degrading marker further enhanced expression.
Language: Английский
Citations
12GZMK+CD8+ T cells Target a Specific Acinar Cell Type in Sjögren’s Disease
Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown
Published: Dec. 19, 2023
Sjögren's Disease (SjD) is a systemic autoimmune disease without clear etiology or effective therapy. Utilizing unbiased single-cell and spatial transcriptomics to analyze human minor salivary glands in health we developed comprehensive understanding of the cellular landscape healthy how that changes SjD patients. We identified novel seromucous acinar cell types population
Language: Английский
Citations
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