Parkin-mediated mitophagy as a potential therapeutic target for intervertebral disc degeneration

Cell Death and Disease, Год журнала: 2018, Номер 9(10)

Опубликована: Сен. 24, 2018

Intervertebral disc degeneration (IDD) is a complicated pathological condition blamed for low back pain. Mitochondrion of vital importance cellular homeostasis, and mitochondrial dysfunction considered to be one the major causes damage. Mitophagy process eliminate impaired mitochondria showed protective effects in various diseases; however, its role IDD still not clear. Here, we explore Parkin-mediated mitophagy IDD. In this study, found that Parkin was upregulated degenerative nucleus pulposus (NP) tissues vivo as well TNF-α stimulated NP cells vitro. Knockdown by siRNA crucial apoptosis mitochondrion homeostasis cells. Further study upregulation salidroside may promote survival through activation could inhibit ameliorate progression These results suggested involved pathogenesis potential therapeutic target

Язык: Английский

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The Potential Use of Mesenchymal Stem Cells and Their Derived Exosomes for Orthopedic Diseases Treatment

Stem Cell Reviews and Reports, Год журнала: 2021, Номер 18(3), С. 933 - 951

Опубликована: Июнь 24, 2021

Язык: Английский

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Extracellular Vesicles as an Emerging Treatment Option for Intervertebral Disc Degeneration: Therapeutic Potential, Translational Pathways, and Regulatory Considerations

Advanced Healthcare Materials, Год журнала: 2021, Номер 11(5)

Опубликована: Июль 23, 2021

Emergent approaches in regenerative medicine look toward the use of extracellular vesicles (EVs) as a next-generation treatment strategy for intervertebral disc (IVD) degeneration (IVDD) because their ability to attenuate chronic inflammation, reduce apoptosis, and stimulate proliferation number tissue systems. Yet, there are no Food Drug Administration (FDA)-approved EV therapeutics market with an indication IVDD, which motivates this article review current state field provide IVD-specific framework assess its efficacy. In systematic review, 29 preclinical studies that investigate EVs relation IVD identified, additionally, regulatory approval process is reviewed effort accelerate emerging EV-based FDA submission timeline-to-market. The majority focus on nucleus pulposus responses treatment, where main findings show stem cell-derived can decelerate progression IVDD molecular, cellular, organ level. also highlight importance parent cell's pathophysiological differentiation state, affects downstream therapeutic outcomes. This substantiates promising cell-free treat enhance endogenous repair.

Язык: Английский

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Exhausted local lactate accumulation via injectable nanozyme-functionalized hydrogel microsphere for inflammation relief and tissue regeneration

Bioactive Materials, Год журнала: 2021, Номер 12, С. 153 - 168

Опубликована: Окт. 22, 2021

Local lactate accumulation greatly hinders tissue repair and regeneration under ischemic condition. Herein, an injectable microsphere (MS@MCL) for local exhaustion was constructed by grafting manganese dioxide (MnO2) -lactate oxidase (LOX) composite nanozyme on microfluidic hyaluronic acid methacrylate (HAMA) microspheres via chemical bonds, achieving a long-term oxygen-promoted effect long half-life in vivo. The uniform porous synthesized technology is beneficial to situ injection therapy improving encapsulation efficiency. Furthermore, into HAMA through amide reactions promoted enzymatic concentration activity enhancement. It showed that the MS@MCL eliminated oxidative inflammatory stress extracellular matrix metabolism cell survival when co-cultured with nucleus pulposus cells (NPCs) vitro. In rat degenerative intervertebral disc model caused injection, therapeutic reducing height narrowing preventing (ECM) degradation as well damage Altogether, this study confirms nanozyme-functionalized effectively improves regenerative reparative tissues disposing of enriched microenvironment.

Язык: Английский

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Berberine ameliorates oxidative stress-induced apoptosis by modulating ER stress and autophagy in human nucleus pulposus cells

Life Sciences, Год журнала: 2019, Номер 228, С. 85 - 97

Опубликована: Апрель 30, 2019

Язык: Английский

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