Protein oxidation - Formation mechanisms, detection and relevance as biomarkers in human diseases

Redox Biology, Год журнала: 2021, Номер 42, С. 101901 - 101901

Опубликована: Фев. 21, 2021

Generation of reactive oxygen species and related oxidants is an inevitable consequence life. Proteins are major targets for oxidation reactions, because their rapid reaction rates with high abundance in cells, extracellular tissues, body fluids. Additionally, oxidative stress able to degrade lipids carbohydrates highly intermediates, which eventually attack proteins at various functional sites. Consequently, a wide variety distinct posttranslational protein modifications formed by oxidation, glycoxidation, lipoxidation. Reversible relevant physiological processes constitute signaling mechanisms ("redox signaling"), while non-reversible may contribute pathological situations several diseases. A rising number publications provide evidence involvement the onset progression diseases as well aging processes. Certain products chemically stable large quantity, makes them promising candidates become biomarkers damage. Moreover, progress development detection quantification methods facilitates analysis time effort contributes future applicability clinical routine. The present review outlines most important classes selected examples modifications, elucidates chemistry beyond formation discusses available analysis. Furthermore, relevance potential context disease summarized.

Язык: Английский

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Protective Effects of Estrogen on Cardiovascular Disease Mediated by Oxidative Stress

Oxidative Medicine and Cellular Longevity, Год журнала: 2021, Номер 2021(1)

Опубликована: Янв. 1, 2021

Perimenopause is an important stage of female senescence. Epidemiological investigation has shown that the incidence cardiovascular disease in premenopausal women lower than men, and postmenopausal significantly higher men. This phenomenon reveals estrogen a definite protective effect on system. In system, oxidative stress considered pathogenesis atherosclerosis, myocardial dysfunction, cardiac hypertrophy, heart failure, ischemia. From perspective stress, plays regulatory role system through receptor, providing strategies for treatment menopausal with diseases.

Язык: Английский

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The Importance of Nitrate Reduction for Oral Health

Journal of Dental Research, Год журнала: 2022, Номер 101(8), С. 887 - 897

Опубликована: Фев. 23, 2022

Salivary glands concentrate plasma nitrate into saliva, leading to high concentrations that can reach the millimolar range after a nitrate-rich vegetable meal. Whereas human cells cannot reduce nitrite effectively, certain oral bacteria can. This leads an increase in systemic improve conditions such as hypertension and diabetes through nitric oxide availability. Apart from benefits, it has been proposed microbial reduction also promote health. In this review, we discuss evidence associating dietary with Oral oxide, free radical antimicrobial properties capable of inhibiting sensitive species anaerobes involved periodontal diseases. Nitrate shown resilience against salivary acidification vivo vitro, thus preventing caries development. One potential mechanism is proton consumption during denitrification and/or bacterial ammonium. Additionally, lactic acid (organic acidification) hydrogen sulfide (volatile compound halitosis) act electron donors for these processes. The nitrate-reducing Rothia Neisseria are consistently found at higher levels individuals disease (vs. caries, periodontitis, when consumed clinical studies. Preliminary vitro show normally associated disease, Veillonella (caries) Prevotella (periodontal diseases halitosis), decrease presence nitrate. We propose ecologic factor stimulating eubiosis (i.e., health-associated functions). Finally, preventive therapeutic potential, well safety issues, related use limited; therefore, robust studies required confirm benefits on

Язык: Английский

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PINK1/Parkin-mediated mitophagy inhibits osteoblast apoptosis induced by advanced oxidation protein products

Cell Death and Disease, Год журнала: 2023, Номер 14(2)

Опубликована: Фев. 7, 2023

Osteoblast apoptosis plays an important role in age-related bone loss and osteoporosis. Our previous study revealed that advanced oxidation protein products (AOPPs) could induce nicotinamide adenine dinucleotide phosphate oxidase (NOX)-derived reactive oxygen species (ROS) production, cause mitochondrial membrane potential (ΔΨm) depolarization, trigger the mitochondria-dependent intrinsic pathway, lead to osteoblast ultimately osteopenia microstructural destruction. In this study, we found AOPPs also induced ROS (mtROS) generation osteoblastic MC3T3-E1 cells, which was closely related NOX-derived ROS, aggravated oxidative stress condition, thereby further promoting apoptosis. Removing excessive damaged mitochondria is key factor reversing AOPP-induced Here, by vitro studies, showed rapamycin activated PINK1/Parkin-mediated mitophagy AOPP-stimulated cells significantly alleviated cell eliminating mitochondria. vivo studies decrease plasma AOPP concentration inhibit apoptosis, thus ameliorating accumulation-related loss, destruction mineral density (BMD) loss. Together, our indicated therapeutic strategies aimed at upregulating preserving function might have for treating

Язык: Английский

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Ozone: a natural bioactive molecule with antioxidant property as potential new strategy in aging and in neurodegenerative disorders

Ageing Research Reviews, Год журнала: 2020, Номер 63, С. 101138 - 101138

Опубликована: Авг. 15, 2020

Systems medicine is founded on a mechanism-based approach and identifies in this way specific therapeutic targets. This has been applied for the transcription factor nuclear (erythroid-derived 2)-like 2 (Nrf2). Nrf2 plays central role different pathologies including neurodegenerative disorders (NDs), which are characterized by common pathogenetic features. We here present wide scientific background indicating how natural bioactive molecule with antioxidant/anti-apoptotic pro-autophagy properties such as ozone (O3) can represent potential new strategy to delay neurodegeneration. Our hypothesis based evidence demonstrating interaction between O3 system. Through meta-analytic approach, we found significant modulation of endogenous antioxidant-Nrf2 (p < 0.00001, Odd Ratio (OR) = 1.71 95%CI:1.17-2.25) vitagene-Nrf2 systems OR 1.80 95%CI:1.05-2.55). activates also immune, anti-inflammatory signalling, proteasome, releases growth factors, improves blood circulation, antimicrobial activity, effects gut microbiota. Thus, provide consistent rationale implement future clinical studies apply oxygen-ozone (O2-O3) therapy an early phase aging decline, when it still possible intervene before potentially develop more severe pathology. suggest that along other antioxidants (polyphenols, mushrooms) implicated same Nrf2-mechanisms, show neurogenic potential, providing preventive strategies NDs.

Язык: Английский

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Oxidative Stress Biomarkers among Schizophrenia Inpatients

Brain Sciences, Год журнала: 2023, Номер 13(3), С. 490 - 490

Опубликована: Март 14, 2023

Finding the associations between schizophrenia symptoms and biomarkers of inflammation, oxidative stress kynurenine pathway may lead to individualization treatment increase its effectiveness.The study group included 82 inpatients. The Positive Negative Symptoms Scale (PANSS), Brief Assessment Cognition in Schizophrenia (BACS) Calgary Depression were used for symptom evaluation. Biochemical analyses parameters brain-derived neurotrophic factor (BDNF).Linear models revealed following: (1) malondiadehyde (MDA), N-formylkynurenine (N-formKYN), advanced oxidation protein products (AOPP), glycation end-products proteins (AGE) total status (TOS) levels are related PANSS-total score; (2) MDA, reduced glutathione (GSH) BDNF PANSS-negative (3) TOS (KYN) PANSS-positive (4) antioxidant (TAS) AOPP along with CDSS score BACS-total (5) TAS N-formKYN BACS-working memory score.Oxidative be associated severity positive, negative cognitive dimensions. identification biochemical markers specific clusters understanding profiles patients.

Язык: Английский

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Salivary Antioxidants and Oxidative Stress in Psoriatic Patients: Can Salivary Total Oxidant Status and Oxidative Status Index Be a Plaque Psoriasis Biomarker?

Oxidative Medicine and Cellular Longevity, Год журнала: 2020, Номер 2020, С. 1 - 12

Опубликована: Янв. 4, 2020

The aim of our research was to evaluate redox balance parameters and biomarkers oxidative stress (OS) in nonstimulated stimulated saliva as well the blood patients with plaque psoriasis compared healthy controls. study involved 40 generally subjects matched by age gender group patients. We assayed concentration/activity antioxidant enzymes: salivary peroxidase (Px), catalase (CAT), superoxide dismutase (SOD) measured unstimulated (NWS), (SWS), erythrocytes. In plasma NWS SWS, we reduced glutathione (GSH), total potential (TAC), status (TOS), index (OSI), markers modification proteins: advanced glycation end products (AGE), oxidation protein (AOPP), lipid products: malondialdehyde (MDA) hydroperoxide (LOOH). also evaluated rate reactive oxygen species (ROS) production. concentration Px, CAT, SOD significantly higher vs. subjects. SWS psoriatic patients, observed considerably Px erythrocytes psoriasis, GPx CAT that levels AOPP, AGE, MDA, LOOH were NWS, amylase lower control. course imbalances prevalence reactions. Mechanisms synthesis/secretion proteins activity depressed both glands patients; however, they more inhibited parotid gland submandibular gland. TOS OSI value may serve diagnostic psoriasis.

Язык: Английский

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Salivary Redox Biomarkers in Selected Neurodegenerative Diseases

Journal of Clinical Medicine, Год журнала: 2020, Номер 9(2), С. 497 - 497

Опубликована: Фев. 12, 2020

Neurodegenerative diseases (NDDs), such as Alzheimer’s disease, Parkinson’s and Huntington’s are disorders, which cause irreversible progressive deterioration of the central nervous system. The pathophysiology NDDs is still not fully explained; nevertheless, oxidative stress considered a critical mediator cerebral degeneration, brain inflammation, well neuronal apoptosis. Therefore, it surprising that redox biomarkers increasingly used in diagnosis neurodegenerative diseases. As saliva very easy to obtain bioliquid, seems promising use this biomaterial NDDs. Saliva collection easy, cheap, stress-free, non-infectious, does require help specialised medical personnel. Additionally, concentrations many salivary correlate with their content blood serum degree disease progression, makes them non-invasive indicators This paper reviews latest knowledge concerning prognosis selected

Язык: Английский

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Salivary Oxidative Stress Increases with the Progression of Chronic Heart Failure

Journal of Clinical Medicine, Год журнала: 2020, Номер 9(3), С. 769 - 769

Опубликована: Март 12, 2020

The aim of the study was to evaluate rate reactive oxygen species (ROS) production, antioxidant barrier, and oxidative damage in non-stimulated (NWS) stimulated (SWS) saliva as well plasma/erythrocytes 50 patients with chronic heart failure (HF) divided into two subgroups: NYHA II (33 patients) III (17 patients). activity superoxide dismutase catalase statistically increased NWS HF compared healthy controls. free radical formation, total oxidant status, level uric acid, advanced glycation end products (AGE), oxidation protein malondialdehyde significantly elevated NWS, SWS, plasma We were first demonstrate that progression HF, disturbances enzymatic non-enzymatic defense, proteins lipids occur at both central (plasma/erythrocytes) local (saliva) levels. In group, we also observed a decrease secretion, salivary amylase age- gender-matched control which indicates secretory dysfunction glands HF. Salivary AGE may be potential biomarker differential diagnosis

Язык: Английский

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Aging-Related Metabolic Dysfunction in the Salivary Gland: A Review of the Literature

International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(11), С. 5835 - 5835

Опубликована: Май 29, 2021

Aging-related salivary dysfunction commonly induces the poor oral health, including decreased saliva flow and dental caries. Although clinical significance of glands is well-known, complex metabolic pathways contributing to aging-dysfunction process are only beginning be uncovered. Here, we provide a comprehensive overview changes in aging-mediated gland as key aspect physiology. Several neuropeptides or hormones involved causing dysfunction, hyposalivation age-related diseases. Thus, aging-related metabolism holds promise for early diagnosis, increased choice therapy identification new that could potentially targeted dysfunction.

Язык: Английский

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