Biomedical Applications of Microfluidic Devices: A Review

Biosensors, Год журнала: 2022, Номер 12(11), С. 1023 - 1023

Опубликована: Ноя. 16, 2022

Both passive and active microfluidic chips are used in many biomedical chemical applications to support fluid mixing, particle manipulations, signal detection. Passive devices geometry-dependent, their uses rather limited. Active include sensors or detectors that transduce chemical, biological, physical changes into electrical optical signals. Also, they transduction detect biological applications, highly versatile tools for disease diagnosis organ modeling. This review provides a comprehensive overview of the significant advances have been made development microfluidics devices. We will discuss function as micromixers sorters cells substances (e.g., microfiltration, flow displacement, trapping). Microfluidic fabricated using range techniques, including molding, etching, three-dimensional printing, nanofabrication. Their broad utility lies detection diagnostic biomarkers organ-on-chip approaches permit modeling cancer, well neurological, cardiovascular, hepatic, pulmonary diseases. Biosensor allow point-of-care testing, assays based on enzymes, nanozymes, antibodies, nucleic acids (DNA RNA). An anticipated field includes optimization techniques fabrication biocompatible materials. These developments increase versatility, reduce costs, accelerate time technology.

Язык: Английский

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Biomaterials for Tissue Engineering Applications and Current Updates in the Field: A Comprehensive Review

AAPS PharmSciTech, Год журнала: 2022, Номер 23(7)

Опубликована: Сен. 26, 2022

Abstract Tissue engineering has emerged as an interesting field nowadays; it focuses on accelerating the auto-healing mechanism of tissues rather than organ transplantation. It involves implanting In Vitro cultured initiative tissue or a scaffold loaded with regenerating ingredients at damaged area. Both techniques are based use biodegradable , biocompatible polymers scaffolding materials which either derived from natural (e.g. alginates, celluloses, and zein) synthetic sources PLGA, PCL, PLA). This review discusses in detail recent applications different biomaterials highlighting targeted besides vitro vivo key findings. As well, smart chitosan) fascinating candidates they capable elucidating chemical physical transformation response to external stimuli temperature, pH, magnetic electric fields). Recent trends summarized this stem cells, 3D printing techniques, most 4D approach relies produce dynamic resembling tissue. Furthermore, application advanced provides hope for researchers recognize COVID-19/host interaction, also, presents promising solution rejuvenate destroyed lung tissues. Graphical abstract

Язык: Английский

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Hydrogel composite scaffolds achieve recruitment and chondrogenesis in cartilage tissue engineering applications

Journal of Nanobiotechnology, Год журнала: 2022, Номер 20(1)

Опубликована: Янв. 6, 2022

The regeneration and repair of articular cartilage remains a major challenge for clinicians scientists due to the poor intrinsic healing this tissue. Since injuries are often clinically irregular, tissue-engineered scaffolds that can be easily molded fill defects any shape fit tightly into host needed.In study, bone marrow mesenchymal stem cell (BMSC) affinity peptide sequence PFSSTKT (PFS)-modified chondrocyte extracellular matrix (ECM) particles combined with GelMA hydrogel were constructed.In vitro experiments showed pore size porosity solid-supported composite appropriate provided three-dimensional microenvironment supporting adhesion, proliferation chondrogenic differentiation. In also GelMA/ECM-PFS could regulate migration rabbit BMSCs. Two weeks after implantation in vivo, functional scaffold system promoted recruitment endogenous cells from defect site. achieved successful hyaline rabbits while control treatment mostly resulted fibrous tissue repair.This combination chondrogenesis is an ideal strategy repairing irregular defects.

Язык: Английский

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Cellular therapy and tissue engineering for cartilage repair

Osteoarthritis and Cartilage, Год журнала: 2022, Номер 30(12), С. 1547 - 1560

Опубликована: Сен. 20, 2022

Articular cartilage (AC) has limited capacity for repair. The first attempt to repair using tissue engineering was reported in 1977. Since then, cell-based interventions have entered clinical practice orthopaedics, and several approaches are the translational pipeline towards application. Classically, these involve a scaffold, substrate or matrix provide structure, cells such as chondrocytes mesenchymal stromal generate tissue. We discuss advantages drawbacks of use various cell types, natural synthetic scaffolds, multiphasic gradient-based self-organizing self-assembling scaffold-free systems, constructs. Several challenges persist including achieving zonal organization integration with surrounding upon implantation. Approaches improve thickness, mechanical properties include stimulation, culture under hypoxic conditions, stimulation growth factors other macromolecules. In addition, advanced technologies bioreactors, biosensors 3D bioprinting actively being explored. Understanding underlying mechanisms action therapy will help refine development. Finally, we recent studies intrinsic cellular molecular that identified novel signals targets inspiring development therapies enhance recruitment reparative activity joint-resident stem progenitor cells. A one-fits-all solution is unrealistic, identifying patients who respond specific targeted treatment be critical.

Язык: Английский

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3D Bioprinting in Otolaryngology: A Review

Advanced Healthcare Materials, Год журнала: 2023, Номер 12(19)

Опубликована: Март 15, 2023

The evolution of tissue engineering and 3D bioprinting has allowed for increased opportunities to generate musculoskeletal grafts that can enhance functional aesthetic outcomes in otolaryngology-head neck surgery. Despite literature reporting successes the fabrication cartilage bone scaffolds applications head neck, full potential this technology yet be realized. Otolaryngology as a field always been at forefront new advancements is well poised spearhead clinical application these engineered tissues. In review, current methods are described an overview cell types, bioinks, bioactive factors available using presented. otologic, nasal, tracheal, craniofacial with focus on graft implantation animal models highlight status vivo; necessary step future translation reviewed. Continued multidisciplinary efforts between material chemistry, biological sciences, otolaryngologists will play key role engineered, bioprinted constructs

Язык: Английский

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Advancements in hydrogel design for articular cartilage regeneration: A comprehensive review

Bioactive Materials, Год журнала: 2024, Номер 43, С. 1 - 31

Опубликована: Сен. 14, 2024

Язык: Английский

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Biomaterial composition and stiffness as decisive properties of 3D bioprinted constructs for type II collagen stimulation

Acta Biomaterialia, Год журнала: 2022, Номер 152, С. 221 - 234

Опубликована: Авг. 30, 2022

Gelatin methacrylate (GelMA) and hyaluronic acid (HAMA) are frequently used biomaterials for 3D bioprinting, with individual well-established material characteristics. To identify an ideal combination of GelMA HAMA chondrogenesis, a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc) was developed. With this non-destructive, high-throughput temporal assay, Gaussia is secreted from the cells as proxy measuring type II collagen production. GelMA:HAMA ratios were screened using before proceeding to bioprinting. This method efficient, saving on time materials, resulting in streamlined process biomaterial optimization. The screen revealed that addition improved chondrogenesis over (15%) alone. Storage moduli measured dynamic mechanical analysis same established initial threshold ∼30kPa. determine if storage impact cell mobility, chondrocytes transduced green fluorescent protein (GFP) bioprinted either 1:1 or 2:1 32kPa 57.9kPa, respectively. We found reduced stiffer biomaterial, had higher expression, than softer more mobility. Finally, after bioprinting HuCol2gLuc we successfully identified optimal (2:1) photo-crosslinking (38s) chondrogenesis. STATEMENT OF SIGNIFICANCE: One challenge identifying stimulate articular cartilage development. gelatin developed (HuCol2gLuc). assay uses streamlines optimization also it level stiffness required And first time, quantified mobility construct. Together these results indicate modulus less improves

Язык: Английский

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Bioreactors for engineering patient-specific tissue grafts

Nature Reviews Bioengineering, Год журнала: 2023, Номер 1(5), С. 361 - 377

Опубликована: Март 2, 2023

Язык: Английский

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Hydrogels in the treatment of rheumatoid arthritis: drug delivery systems and artificial matrices for dynamic in vitro models

Journal of Materials Science Materials in Medicine, Год журнала: 2021, Номер 32(7)

Опубликована: Июнь 22, 2021

Abstract Rheumatoid arthritis (RA) is an autoimmune and chronic inflammatory disorder that mostly affects the synovial joints can promote both cartilage bone tissue destruction. Several conservative treatments are available to relieve pain control inflammation; however, traditional drugs administration not fully effective present severe undesired side effects. Hydrogels a very attractive platform as drug delivery system guarantee these handicaps reduced, therapeutic effect from maximized. Furthermore, hydrogels mimic physiological microenvironment have mechanical behavior needed for use in vitro model. The testing of advanced systems still bound animal disease models shown low predictability. Alternatively, hydrogel-based human dynamic be used model diseases, bypassing some problems. RA embryonary stage since advances regarding healthy inflamed currently giving first steps complexity increase. Herein, recent studies using treatment RA, featuring different hydrogel formulations discussed. Besides, their artificial extracellular matrices articular also reviewed.

Язык: Английский

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Three-dimensional bio-printing of decellularized extracellular matrix-based bio-inks for cartilage regeneration: a systematic review.

PubMed, Год журнала: 2022, Номер 3(2), С. 105 - 115

Опубликована: Янв. 1, 2022

Cartilage injuries are common problems that increase with the population aging. is an avascular tissue a relatively low level of cellular mitotic activity, which makes it impossible to heal spontaneously. To compensate for this problem, three-dimensional bio-printing has attracted great deal attention in cartilage engineering. This emerging technology aims create functional scaffolds by accurately depositing layer-by-layer bio-inks composed biomaterial and cells. As novel bio-ink, decellularized extracellular matrix can serve as appropriate substrate contains all necessary biological cues interactions. Here, review intended provide overview matrix-based their properties, sources, preparation process. Following this, engineering discussed, emphasizing cell behavior in-vivo applications. Afterward, current challenges future outlook will be discussed determine conclusing remarks.

Язык: Английский

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