Novel Trends in Hydrogel Development for Biomedical Applications: A Review

Polymers, Год журнала: 2022, Номер 14(15), С. 3023 - 3023

Опубликована: Июль 26, 2022

Nowadays, there are still numerous challenges for well-known biomedical applications, such as tissue engineering (TE), wound healing and controlled drug delivery, which must be faced solved. Hydrogels have been proposed excellent candidates these they promising properties the mentioned including biocompatibility, biodegradability, great absorption capacity tunable mechanical properties. However, depending on material or manufacturing method, resulting hydrogel may not up to specific task it is designed, thus different approaches enhance performance requirements of application in question. The main purpose this review article was summarize most recent trends technology, going through used polymeric materials popular synthesis methods years, strategies enhancing hydrogels' properties, cross-linking manufacture composite hydrogels. In addition, secondary objective briefly discuss other novel applications hydrogels that past few years drawn a lot attention.

Язык: Английский

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Gelatin Methacryloyl Hydrogel, from Standardization, Performance, to Biomedical Application

Advanced Healthcare Materials, Год журнала: 2023, Номер 12(23)

Опубликована: Апрель 28, 2023

Gelatin methacryloyl (GelMA), a photocurable hydrogel, is widely used in 3D culture, particularly bioprinting, due to its high biocompatibility, tunable physicochemical properties, and excellent formability. However, as the properties performances of GelMA vary under different synthetic conditions, there lack standardization, leading conflicting results. In this study, uniform standard established understand enhance applications. First, basic concept density molecular network (DMN) are defined. Second, two degrees substitution ratio solid content, main measurable parameters determining DMN used. Third, mechanisms relationships between performance various applications terms porosity, viscosity, formability, mechanical strength, swelling, biodegradation, cytocompatibility theoretically explained. The questions that answered: what does mean, why it important, how optimize improve performance, characterize reasonably accurately? Finally, hoped knowledge will eliminate need for researchers conduct tedious repetitive pre-experiments, enable easy communication achievements groups same standard, fully explore potential hydrogel.

Язык: Английский

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Harnessing knee joint resident mesenchymal stem cells in cartilage tissue engineering

Acta Biomaterialia, Год журнала: 2023, Номер 168, С. 372 - 387

Опубликована: Июль 21, 2023

Osteoarthritis (OA) is a widespread clinical disease characterized by cartilage degeneration in middle-aged and elderly people. Currently, there no effective treatment for OA apart from total joint replacement advanced stages. Mesenchymal stem cells (MSCs) are type of adult cell with diverse differentiation capabilities immunomodulatory potentials. MSCs known to effectively regulate the microenvironment, promote regeneration, alleviate symptoms. As result, they promising sources therapy. Recent studies have revealed presence resident synovial fluid, membrane, articular cartilage, which can be collected as knee joint-derived (KJD-MSC). Several preclinical demonstrated that KJD-MSCs great potential treatment, whether applied alone, combination biomaterials, or exocrine MSCs. In this article, we will review characteristics joints, including their cytological characteristics, such proliferation, differentiation, abilities, well biological function MSC exosomes. We also discuss use tissue engineering introduce concept new generation cell-based therapy, engineering, gene editing techniques create KJD-MSC derivative exosomes improved functionality targeted delivery. These advances aim maximize efficiency provide strategies overcome bottleneck This research insights into medicinal benefit Joint Stem Cells (MSCs), specifically on its ability. Through review, community further realize promoting mesenchymal cells, especially progenitor/MSC-like progenitor (CPSC), preventive measure against osteoarthritis injury. People medical institutions may consider derived an alternative approach degeneration. Moreover, discussion presented study convey valuable information future explore benefits MSC.

Язык: Английский

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Strategies of functionalized GelMA-based bioinks for bone regeneration: Recent advances and future perspectives

Bioactive Materials, Год журнала: 2024, Номер 38, С. 346 - 373

Опубликована: Май 9, 2024

Gelatin methacryloyl (GelMA) hydrogels is a widely used bioink because of its good biological properties and tunable physicochemical properties, which has been in variety tissue engineering regeneration. However, pure GelMA limited by the weak mechanical strength lack continuous osteogenic induction environment, difficult to meet needs bone repair. Moreover, are unable respond complex stimuli therefore adapt physiological pathological microenvironments. This review focused on functionalization strategies hydrogel based bioinks for The synthesis process was described details, various functional methods requirements regeneration, including strength, porosity, vascularization, differentiation, immunoregulation patient specific repair, etc. In addition, response smart GelMA-based external physical stimulation internal microenvironment stimulation, as well achieve both disease treatment regeneration presence common diseases (such inflammation, infection, tumor) also briefly reviewed. Finally, we emphasized current challenges possible exploration directions

Язык: Английский

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Research Progress in Hydrogels for Cartilage Organoids

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(22)

Опубликована: Май 21, 2024

The repair and regeneration of cartilage has always been a hot topic in medical research. Cartilage organoids (CORGs) are special tissue created using engineering techniques outside the body. These engineered tissues provide models that simulate complex biological functions cartilage, opening new possibilities for regenerative medicine treatment strategies. However, it is crucial to establish suitable matrix scaffolds cultivation CORGs. In recent years, utilizing hydrogel culture stem cells induce their differentiation into chondrocytes emerged as promising method vitro construction this review, methods establishing CORGs summarized an overview advantages limitations matrigel such provided. Furthermore, importance ECM alternative substitutes Matrigel, alginate, peptides, silk fibroin, DNA derivatives discussed, pros cons these hydrogels outlined. Finally, challenges future directions research discussed. It hoped article provides valuable references design development

Язык: Английский

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Advancements in tissue engineering for articular cartilage regeneration

Heliyon, Год журнала: 2024, Номер 10(3), С. e25400 - e25400

Опубликована: Фев. 1, 2024

Articular cartilage injury is a prevalent clinical condition resulting from trauma, tumors, infection, osteoarthritis, and other factors. The intrinsic lack of blood vessels, nerves, lymphatic vessels within tissue severely limits its self-regenerative capacity after injury. Current treatment options, such as conservative drug therapy joint replacement, have inherent limitations. Achieving perfect regeneration repair articular remains an ongoing challenge in the field regenerative medicine. Tissue engineering has emerged key focus research, aiming to utilize cultured expanded cells combined with suitable scaffold materials create viable, functional tissues. This review article encompasses latest advancements seed cells, scaffolds, cytokines. Additionally, role stimulatory factors including cytokines growth factors, genetic techniques, biophysical stimulation, bioreactor systems, well scaffolding natural synthetic nanostructured scaffolds tissues are discussed. Finally, we also outline signaling pathways involved regeneration. Our provides valuable insights for scholars address complex problem repair.

Язык: Английский

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Recent advancements in cartilage tissue engineering innovation and translation

Nature Reviews Rheumatology, Год журнала: 2024, Номер 20(6), С. 323 - 346

Опубликована: Май 13, 2024

Язык: Английский

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Injectable hydrogels as promising in situ therapeutic platform for cartilage tissue engineering

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 261, С. 129537 - 129537

Опубликована: Янв. 25, 2024

Язык: Английский

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Functional acellular matrix for tissue repair

Materials Today Bio, Год журнала: 2022, Номер 18, С. 100530 - 100530

Опубликована: Дек. 28, 2022

In view of their low immunogenicity, biomimetic internal environment, tissue- and organ-like physicochemical properties, functionalization potential, decellularized extracellular matrix (dECM) materials attract considerable attention are widely used in tissue engineering. This review describes the composition matrices role stem-cell differentiation, discusses advantages disadvantages existing decellularization techniques, presents methods for characterization scaffolds. addition, we discuss progress use dECMs cartilage, skin, nerve, muscle repair transplantation or regeneration different whole organs (e.g., kidneys, liver, uterus, lungs, heart), summarize shortcomings using organ after refunctionalization, examine corresponding future prospects. Thus, present helps to further systematize application functionalized tissue/organ keep researchers up date on recent dECM usage.

Язык: Английский

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An injectable photo-cross-linking silk hydrogel system augments diabetic wound healing in orthopaedic surgery through spatiotemporal immunomodulation

Journal of Nanobiotechnology, Год журнала: 2022, Номер 20(1)

Опубликована: Май 14, 2022

The complicated hyperglycaemic and chronic inflammation of diabetic wounds in orthopaedic surgery leads to dysregulated immune cell function potential infection risk. Immune interventions face a possible contradiction between simultaneous establishment the pro-inflammatory microenvironment response bacterial invasion anti-inflammatory required for tissue repair. To study this accelerate diabetic-wound healing, we developed photocurable methacryloxylated silk fibroin hydrogel (Sil-MA) system, co-encapsulated with metformin-loaded mesoporous silica microspheres (MET@MSNs) silver nanoparticles (Ag NPs).The system (M@M-Ag-Sil-MA) enhanced healing via spatiotemporal immunomodulation. Sil-MA imparts rapid situ Ultra-Violet-photocurable capability allows preliminary controlled release Ag NPs, which can inhibit aggregation create stable, sterile microenvironment. results confirmed involvement Met immunomodulatory effects following dual-controlled Sil-MA. Hysteresis-released from shifts M1 phenotype macrophages regions trauma an M2 phenotype. Simultaneously, M@M-Ag-Sil-MA inhibited formation neutrophil extracellular traps (NETs) decreased elastase, myeloperoxidase, NETs-induced factors. As result modulating microenvironmental, promoted fibroblast migration endothelial angiogenesis vivo, verification accompanied immunoregulation NETs mouse model.Our findings demonstrated that resolved through immunomodulation NETs, suggesting its as promising engineered nano-dressing treatment surgery.

Язык: Английский

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