International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(20), С. 15256 - 15256
Опубликована: Окт. 17, 2023
Dysbiosis,
generally
defined
as
the
disruption
to
gut
microbiota
composition
or
function,
is
observed
in
most
diseases,
including
allergies,
cancer,
metabolic
neurological
disorders
and
diseases
associated
with
autoimmunity.
Dysbiosis
commonly
reduced
levels
of
beneficial
microbiota-derived
metabolites
such
short-chain
fatty
acids
(SCFA)
indoles.
Supplementation
these
metabolites,
interventions
increase
their
microbial
production,
has
been
shown
ameliorate
a
variety
inflammatory
diseases.
Conversely,
production
'dysbiotic'
by-products
by
may
contribute
disease
development.
This
review
summarizes
various
gut-derived
products
cardiovascular
bowel
disease,
non-alcoholic
steatohepatitis
autoimmune
multiple
sclerosis.
The
increased
dysbiotic
products,
trimethylamine,
hydrogen
sulphide,
amino
acid
metabolism
p-Cresyl
sulphate
phenylacetic
acid,
secondary
bile
deoxycholic
across
simultaneous
impaired
modern
lifestyle,
partially
explain
high
prevalence
western
countries.
Frontiers in Immunology,
Год журнала:
2023,
Номер
14
Опубликована: Сен. 4, 2023
To
determine
the
roles
of
endoplasmic
reticulum
(ER)
stress
and
trained
immunity,
we
performed
transcriptome
analyses
on
thoracic
aorta
(TA)
abdominal
(AA)
from
angiotensin
II
(Ang
II)-HFD-ApoE-KO
aneurysm
model
made
significant
findings:
1)
Ang
bypassed
HFD-induced
metabolic
reprogramming
induced
stronger
inflammation
in
AA
than
TA;
2)
HFD
upregulated
890
genes
versus
TA
cytokine
signaling;
3)
73
68
cytokines,
scRNA-Seq
identified
markers
macrophages
immune
cells,
cell
death
regulators,
respectively;
transdifferentiation
neuron,
glial,
squamous
epithelial
cells
were
by
II-AA
pyroptosis
signaling
with
IL-1β
caspase-4
more
4)
Six
transcriptomes
patients
AAA,
AA,
TA,
additional
models,
PPE-AAA
BAPN-Ang
II-AAA,
partially
overlapped
10
lists
new
ER
gene
sets
including
3
interaction
protein
regulators
ATF6,
PERK,
IRE1,
HPA
localization
genes,
KEGG
signal
XBP1
transcription
targets,
ATF4
(PERK)
ATF6
thapsigargin
tunicamycin-ER
5)
ROS
MitoCarta
immunity
glycolysis
genes;
6)
Gene
KO
indicated
that
PERK
played
IRE1
promoting
AAA
whereas
antioxidant
NRF2
inhibited
them.
Our
unprecedented
ER-focused
transcriptomic
have
provided
novel
insights
as
an
organelle
sensing
various
DAMPs
initiating
triggers
II-accelerated
differs
susceptibilities
aortas
to
diseases.
Frontiers in Pharmacology,
Год журнала:
2024,
Номер
15
Опубликована: Июнь 20, 2024
Heart
failure
(HF)
is
a
chronic
condition
that
progressively
worsens
and
continues
to
be
major
financial
burden
public
health
concern.
The
“gut-heart”
axis
provides
an
innovative
perspective
therapeutic
strategy
for
preventing
treating
heart
failure.
Shenfu
injection
(SFI)
Traditional
Chinese
Medicine-based
treatment
demonstrating
potential
as
However,
the
precise
mechanisms
of
SFI
in
are
not
completely
characterized.
In
this
study,
HF
models
were
established
utilizing
subcutaneous
multipoint
isoproterenol
(ISO)
at
dosage
5
mg
kg
−1
·d
7
days.
Serum
levels
inflammatory
biomarkers
quantified
using
protein
microarrays.
Rat
feces
analyzed
untargeted
metabolomics
research
16S
rRNA
sequencing.
link
between
gut
microbiota
metabolites
was
examined
MetOrigin
Spearman
correlation
analysis.
Our
results
show
effectively
enhances
cardiac
function
rats
with
ISO-induced
by
potentially
modulating
pro-/anti-inflammatory
imbalance
reducing
serum
urine
Trimethylamine-N-oxide
(TMAO)
levels.
Moreover,
significantly
increases
abundance
Bacteroidota
phylum
level,
thereby
improving
disrupted
composition.
Additionally,
supplementation
enriches
specific
genera
known
their
capacity
produce
short-chain
fatty
acids.
found
associated
three
key
metabolic
pathways,
revealed
fecal
metabonomics
analysis,
including
pentose
phosphate
pathway,
pyrimidine
metabolism,
purine
metabolism.
Metabolite
tracing
analysis
Taurine
hypotaurine
metabolism
microbial
community.
biosynthesis
Pyrimidine
Purine
beta-alanine
Naphthalene
degradation,
Pantothenate,
CoA
identified
co-metabolic
pathways
microbes
host.
also
correlated
differentially
expressed
regulated
microbiota.
These
suggest
improves
co-metabolism
regulating
TMAO-inflammation
axis.
Heliyon,
Год журнала:
2024,
Номер
10(15), С. e35461 - e35461
Опубликована: Июль 30, 2024
Trimethylamine-N-oxide
(TMAO)
is
a
harmful
metabolite
dependent
on
the
intestinal
microbiota
and
excreted
through
kidneys.
According
to
numerous
investigations,
rich
circulation
concentrations
of
TMAO
have
been
linked
kidney
gastrointestinal
disorders.
Through
"gut-kidney
axis"
mediated
by
TMAO,
this
research
attempted
clarify
microbiological
causes
kidney-yang
deficiency
syndrome
diarrhea.
To
determine
whether
hyperlipidemia
and
chronic
kidney
disease
(CKD)
have
a
synergy
in
accelerating
vascular
inflammation
via
trained
immunity
(TI),
we
performed
aortic
pathological
analysis
RNA-sequencing
of
high-fat
diet
(HFD)-fed
5/6
nephrectomy
CKD
(HFD+CKD)
mice.
We
made
the
following
findings:
1)
HFD+CKD
increased
cytosolic
lipopolysaccharide
(LPS)
levels,
caspase-11
(CASP11)
activation,
998
gene
expressions
TI
pathways
aorta
(first-tier
mechanism);
2)
CASP11–/–
decreased
neointima
hyperplasia,
recruitment
macrophages,
casp11-gasdermin
D-mediated
cytokine
secretion;
3)
N-terminal
gasdermin
D
(N-GSDMD)
membrane
expression
on
endothelial
cells
IL-1B
levels;
4)
LPS
transfection
into
human
resulted
CASP4
(human)/CASP11
(mouse)
activation
N-GSDMD
expression;
5)
served
as
second-tier
mechanism
underlying
HFD+CKD-promoted
TI.
Taken
together,
accelerated
by
promoting
two-tier
immunity.
Redox Biology,
Год журнала:
2024,
Номер
76, С. 103331 - 103331
Опубликована: Авг. 29, 2024
Mitochondria,
traditionally
recognized
as
cellular
'powerhouses'
due
to
their
pivotal
role
in
energy
production,
have
emerged
multifunctional
organelles
at
the
intersection
of
bioenergetics,
metabolic
signaling,
and
immunity.
However,
understanding
exact
contributions
immunity
inflammation
is
still
developing.
This
review
first
introduces
innovative
concept
intracellular
immunity,
emphasizing
how
mitochondria
serve
critical
immune
signaling
hubs.
They
are
instrumental
recognizing
responding
pathogen
danger
signals,
modulating
responses.
We
also
propose
leading
organelles,
drawing
parallels
with
broader
system
functions
antigen
presentation,
regulation,
response.
Our
comprehensive
explores
mitochondrial
pathways,
therapeutic
potential
managing
chronic
diseases,
discusses
cutting-edge
methodologies
for
research.
Targeting
a
broad
readership
both
experts
newcomers
field,
this
sets
forth
new
directions
that
could
transform
our
integrated
organelles.
International Dental Journal,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Periodontitis
(PD)
is
a
common
chronic
inflammatory
oral
disease
that
severely
affects
patients'
quality
of
life.
Fisetin
has
been
shown
to
possess
antioxidant
and
anti-inflammatory
properties
in
various
biological
systems.
Trimethylamine
N-oxide
(TMAO)
is
a
metabolite
produced
by
gut
microbiota,
and
its
potential
impact
on
lipid
metabolism
in
mammals
has
garnered
widespread
attention
the
scientific
community.
Bovine
fatty
liver
disease,
metabolic
disorder
that
severely
affects
health
productivity
of
dairy
cows,
poses
significant
economic
burden
global
industry.
However,
specific
role
pathogenesis
TMAO
bovine
disease
remain
unclear,
limiting
our
understanding
treatment
condition.
This
study
aims
to
construct
cell
model
using
an
integrated
approach
combines
transcriptomic,
proteomic,
metabolomic
data.
The
objective
investigate
at
molecular
level
explore
regulatory
mechanisms.
We
established
vitro
conducted
comprehensive
analysis
cells
treated
with
high-throughput
omics
sequencing
technologies.
Bioinformatics
methods
were
employed
delve
into
effects
metabolism,
several
key
genes
validated
through
RT-qPCR.
Treatment
significantly
affected
4790
genes,
397
proteins,
137
metabolites.
KEGG
enrichment
revealed
altered
molecules
primarily
involved
pathways
related
pathology
such
as
pathways,
insulin
resistance,
hepatitis
B,
AMPK
signaling
pathway.
Moreover,
joint
analysis,
we
further
uncovered
interaction
between
TMAO-mediated
oxidative
phosphorylation
might
be
mechanism
promoting
accumulation
liver.
Our
provides
new
insights
offers
basis
for
developing
more
effective
strategies.
