BIFROST: A method for registering diverse imaging datasets of the Drosophila brain DOI Creative Commons

Bella E. Brezovec,

Andrew B. Berger,

Yukun A. Hao

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(47)

Published: Nov. 14, 2024

Imaging methods that span both functional measures in living tissue and anatomical fixed have played critical roles advancing our understanding of the brain. However, making direct comparisons between different imaging modalities, particularly spanning tissue, has remained challenging. For example, comparing brain-wide neural dynamics across experiments aligning such data to resources, as gene expression patterns or connectomes, requires precise alignment a common set coordinates. reaching this goal is difficult because registering vivo ex reference atlases accommodating differences modality, microscope specification, sample preparation. We overcome these challenges

Language: Английский

A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution DOI Creative Commons
Geoffrey W. Meissner, Aljoscha Nern, Zachary Dorman

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Feb. 23, 2023

Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of

Language: Английский

Citations

89
Converting an allocentric goal into an egocentric steering signal DOI Creative Commons
Peter Mussells Pires, Lingwei Zhang,

Victoria Parache

et al.

Nature, Journal Year: 2024, Volume and Issue: 626(8000), P. 808 - 818

Published: Feb. 7, 2024

Neuronal signals that are relevant for spatial navigation have been described in many species

Language: Английский

Citations

46
A split-GAL4 driver line resource for Drosophila neuron types DOI Creative Commons
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: Jan. 24, 2025

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system (CNS) have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult CNS 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal cell-type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Language: Английский

Citations

3
An unbiased template of the Drosophila brain and ventral nerve cord DOI Creative Commons
John Bogovic, Hideo Otsuna, Larissa Heinrich

et al.

PLoS ONE, Journal Year: 2020, Volume and Issue: 15(12), P. e0236495 - e0236495

Published: Dec. 31, 2020

The fruit fly Drosophila melanogaster is an important model organism for neuroscience with a wide array of genetic tools that enable the mapping individual neurons and neural subtypes. Brain templates are essential comparative biological studies because they analyzing many individuals in common reference space. Several central brain exist Drosophila, but every one either biased, uses sub-optimal tissue preparation, imaged at low resolution, or does not account artifacts. No publicly available ventral nerve cord template currently exists. In this work, we created high-resolution using best-available technologies imaging, artifact correction, stitching, construction groupwise registration. We evaluated our against four most competitive, demonstrate ours enables more accurate registration fewer local deformations shorter time.

Language: Английский

Citations

124
Connectomics and the neural basis of behaviour DOI Creative Commons
Dana S. Galili, Gregory S.X.E. Jefferis, Marta Costa

et al.

Current Opinion in Insect Science, Journal Year: 2022, Volume and Issue: 54, P. 100968 - 100968

Published: Sept. 13, 2022

Methods to acquire and process synaptic-resolution electron-microscopy datasets have progressed very rapidly, allowing production annotation of larger, more complete connectomes. More accurate neuronal matching techniques are enriching cell type data with gene expression, neuron activity, behaviour developmental information, providing ways test hypotheses circuit function. In a variety behaviours such as learned innate olfaction, navigation sexual behaviour, connectomics has already revealed interconnected modules hierarchical structure, recurrence integration sensory streams. Comparing individual connectomes determine which features robust variable is one key research area; new work in comparative across development, experience, sex species will establish strong links between connectivity brain

Language: Английский

Citations

44
Virtual Fly Brain—An interactive atlas of the Drosophila nervous system DOI Creative Commons
Robert Court, Marta Costa, Clare Pilgrim

et al.

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 14

Published: Jan. 26, 2023

As a model organism,

Language: Английский

Citations

40
Comparative connectomics of the descending and ascending neurons of theDrosophilanervous system: stereotypy and sexual dimorphism DOI Creative Commons
Tomke Stürner, P. D. Brooks, Laia Serratosa Capdevila

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 6, 2024

In most complex nervous systems there is a clear anatomical separation between the nerve cord, which contains of final motor outputs necessary for behaviour, and brain. insects, neck connective both physical information bottleneck connecting brain ventral cord (VNC, spinal analogue) comprises diverse populations descending (DN), ascending (AN) sensory neurons, are crucial sensorimotor signalling control. Integrating three separate EM datasets, we now provide complete connectomic description neurons female system

Language: Английский

Citations

13
A split-GAL4 driver line resource forDrosophilaneuron types DOI Creative Commons
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 10, 2024

Abstract Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Language: Английский

Citations

12
Long-term neuropeptide modulation of female sexual drive via the TRP channel in Drosophila melanogaster DOI Creative Commons
Do‐Hyoung Kim, Yong-Hoon Jang, Minsik Yun

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(10)

Published: Feb. 27, 2024

Connectomics research has made it more feasible to explore how neural circuits can generate multiple outputs. Female sexual drive provides a good model for understanding reversible, long-term functional changes in motivational circuits. After emerging, female flies avoid male courtship, but they become sexually receptive over 2 d. Mating causes females reject further mating several days. Here, we report that pC1 neurons, which process courtship and regulate copulation behavior, exhibit increased CREB (cAMP response element binding protein) activity during maturation decreased after mating. This requires the neuropeptide Dh44 (Diuretic hormone 44) its receptors. A subset of neurons secretes Dh44, stimulates increases expression TRP channel Pyrexia (Pyx) neurons. This, turn, excitability drive. suppresses pyx excitability. is orthologous conserved corticotrophin-releasing family, suggesting similar roles other species.

Language: Английский

Citations

8
A central steering circuit inDrosophila DOI Creative Commons
Kai Feng, Mariam Khan, Ryo Minegishi

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 2, 2024

Abstract Locomotion steering control enables animals to pursue targets, evade threats, avoid obstacles, and explore their environment. Steering commands are generated in the brain communicated via descending neurons leg or wing motor circuits. The diversity of ways which turns triggered executed has led view that might rely on distributed neural processing across multiple Here, however, we present evidence for a central circuit Drosophila is used both goal-directed exploratory capable eliciting ranging from subtle course corrections rapid saccades. organized hierarchy, top layer comprises reciprocally connected DNa03 LAL013 neurons. Our data suggest initiated by reinforced stabilized through winner-take-all mechanism involving LAL013. DNa11 form an intermediate layer. They receive input target circuits directly as well indirectly subordinate activation coordinately changes stepping directions all six legs generate saccadic turns. Together, these define flexibly fly exploits explores its

Language: Английский

Citations

8