The seminal odorant binding protein Obp56g is required for mating plug formation and male fertility in Drosophila melanogaster

eLife, Год журнала: 2023, Номер 12

Опубликована: Дек. 21, 2023

In Drosophila melanogaster and other insects, the seminal fluid proteins (SFPs) male sex pheromones that enter female with sperm during mating are essential for fertility induce profound post-mating effects on physiology. The SFPs in D. taxa include several members of large gene family known as odorant binding (Obps). Work has shown some Obp genes highly expressed antennae can mediate behavioral responses to odorants, potentially by carrying these molecules receptors. These observations have led hypothesis Obps might act molecular carriers or compounds important fertility, though functional evidence any species is lacking. Here, we used genetics test role seven response (PMR). We found Obp56g required induction PMR, whereas six dispensable. males lacking fail form a plug mated female's reproductive tract, leading ejaculate loss reduced storage, likely due its expression ejaculatory bulb. also examined evolutionary history genes, studies documented rapid evolution turnover SFP across taxa. extensive lability copy number positive selection acting two Obp22a Obp51a. Comparative RNAseq data from tract multiple revealed shows high subset taxa, conserved head phylogeny. Together, suggest may been co-opted function over time.

Язык: Английский

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Evolution and genetics of accessory gland transcriptome divergence between Drosophila melanogaster and D. simulans

Genetics, Год журнала: 2024, Номер 227(2)

Опубликована: Март 22, 2024

Abstract Studies of allele-specific expression in interspecific hybrids have provided important insights into gene-regulatory divergence and hybrid incompatibilities. Many such investigations Drosophila used transcriptome data from complex mixtures many tissues or gonads, however, regulatory may vary widely among species, sexes, tissues. Thus, we lack sufficiently broad sampling to be confident about the general biological principles divergence. Here, seek fill some these gaps literature by characterizing evolution misexpression a somatic male sex organ, accessory gland, F1 between melanogaster D. simulans. The gland produces seminal fluid proteins, which play an role female fertility subject adaptive due male–male male–female interactions. We find that trans differences are relatively more abundant than cis, contrast most literature, though large effect-size rare. Seminal protein genes significantly elevated levels tend regulated through both cis limited (over- underexpression relative parents) this organ compared other studies. As previous studies, male-biased overrepresented misexpressed much likely underexpressed. ATAC-Seq show chromatin accessibility is correlated with species expression. This work identifies unique properties suggests importance tissue-specific

Язык: Английский

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Male-derived transcripts isolated from the mated female reproductive tract in Drosophila melanogaster

G3 Genes Genomes Genetics, Год журнала: 2023, Номер 13(11)

Опубликована: Сен. 19, 2023

In species with internal fertilization, sperm, and seminal fluid are transferred from male to female during mating. While both sperm contain various types of molecules, including RNA, the role most these molecules in coordination fertilization or other possible functions is poorly understood. Drosophila, exosomes accessory gland, which produces fluid, females, but their potential cargoes have not been described. Moreover, while RNA composition has described several mammalian species, little work on this problem occurred Drosophila. Here we use single nucleotide polymorphism differences between males females a set highly inbred lines D. melanogaster, transcriptome data reproductive tract, testis, investigate origin, vs female, isolated 3 tract organs, receptacle spermatheca, store parovaria, does not. We find that mated carry male-derived transcripts many genes, those markers gland known proteins. Our observations also support idea intact can be storage organs.

Язык: Английский

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The carboxypeptidase B and carbonic anhydrase genes play a reproductive regulatory role during multiple matings in Ophraella communa

Frontiers in Molecular Biosciences, Год журнала: 2023, Номер 10

Опубликована: Май 17, 2023

Seminal fluid proteins (SFPs) are key factors in sexual reproduction and transferred to females during mating with sperm. SFPs have a nutritional value because they protect activate sperm storage release optimize fecundity. Multiple matings promote ovipositioning several insect species. Therefore, insects may obtain more SFP through multiple maximize reproduction, but this process has not yet been clearly confirmed. Here, the relationship between Ophraella communa (Coleoptera: Chrysomelidae), biological control agent of common ragweed Ambrosia artemisiifolia (Asterales: Asteraceae), was studied. significantly increased female fecundity ovary egg deposition. Carboxypeptidase B (OcCpb) carbonic anhydrase (OcCa) genes were identified as putative O. showed strong male-biased expression. Additionally, OcCpb OcCa expression upregulated bursa copulatrix compared that virgin females, their gradually declined after copulation. Furthermore, knockdown males led decrease control. The reproductive tract mated dsRNA-treated dissected observed and, notably, ovaries produced fewer eggs. These data suggest play regulatory roles communa.

Язык: Английский

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Long mounting with repeated copulations in Zygogramma bicolorata: A test of adaptive significance of a curious mating behaviour

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 11, 2024

Long matings are abundant in insects despite the range of costs involved. The causes and consequences evolution long remain an interesting problem for behavioural ecologists. We studied Parthenium beetle (Zygogramma bicolorata), which is known to show extraordinarily copulations. Our detailed investigation showed, first time, that mounting this species entails repeated copulation. Each these copulations preceded succeeded by a leg-rubbing behaviour. found same characteristic pattern laboratory semi-natural conditions. conducted series interrupted assays examine fitness duration mounting. tested multiple hypotheses regarding transfer sperm non-sperm components, function leg rubbing modulating female copulatory Sperm fertility did not exhibit linear increase with number There was also no evidence nutrient males. In contrast, our experiments reveal significant cost beetles. showed remating behaviour can be modulated previous data suggest may reduce resistance mating. While mating possibly serves as mate guarding, males their competitive ability through remating. propose courtship study provides preliminary insights into such systems necessitates further research on sexual selection.

Язык: Английский

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Genotype-by-environment interactions influence the composition of the Drosophila seminal proteome

Proceedings of the Royal Society B Biological Sciences, Год журнала: 2023, Номер 290(2006)

Опубликована: Сен. 13, 2023

Ejaculate proteins are key mediators of post-mating sexual selection and conflict, as they can influence both male fertilization success female reproductive physiology. However, the extent sources genetic variation condition dependence ejaculate proteome largely unknown. Such knowledge could reveal targets mechanisms inform about relative costs allocation different components, each with its own potential fitness consequences. Here, we used liquid chromatography coupled tandem mass spectrometry to characterize whole-ejaculate protein composition across 12 isogenic lines Drosophila melanogaster that were reared on a high- or low-quality diet. We discovered new in transferred inferred their origin system. further found was mainly determined by genotype identity genotype-specific responses larval diet, no clear overall diet effect. Nutrient restriction increased proteolytic activity shifted balance between function RNA metabolism. Our results open avenues for exploring intricate role genotypes environment shaping composition, studying functional dynamics evolutionary multivariate complexity.

Язык: Английский

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The effect of seminal fluid gene expression on paternity

Evolution Letters, Год журнала: 2023, Номер 7(5), С. 361 - 369

Опубликована: Авг. 7, 2023

When females mate with more than one male, competition between rival ejaculates is expected to favor adaptations that promote fertilization success. There now compelling evidence sperm selects for increased production and allocation of sperm. However, comes packaged in also contain protein-rich seminal fluids. Predicting how males should allocate individual fluid proteins response hampered by our limited knowledge their precise function. We use gene expression studies interference RNA ask the ejaculate a cricket,

Язык: Английский

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Does perception of female cues modulate male short‐term fitness components in Drosophila melanogaster?

Ecology and Evolution, Год журнала: 2022, Номер 12(9)

Опубликована: Сен. 1, 2022

Phenotypic plasticity in reproductive behavior can be a strong driver of individual fitness. In species with high intra-sexual competition, changes socio-sexual context trigger quick adaptive plastic responses males. particular, recent study the vinegar fly (

Язык: Английский

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A covariant model of molecular and diversification patterns through time and the history of large clades

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 6, 2024

A bstract Rate shifts in speciation and extinction have been recognised as important contributors to the creation of evolutionary patterns. In particular, distribution modern clade sizes is difficult reconcile with models that do not include them. Although recent advances allowed rate be integrated into models, these largely for purpose inferring historical across phylogenetic trees. addition, typically assumed an independence between patterns diversification rates molecular morphological evolution, despite there being mounting evidence a connection Here, we develop new model two principal goals: first, explore general implied by constantly changing rates, secondly integrate diversification, evolution single coherent framework. We thus analyse covariant birth-death process which all processes (i.e. speciation, change) covary continuously, both each species through time. use this show diversity likely dominated small number extremely large clades at any epoch; are expected characterised explosive early radiations accompanied elevated evolution; extant organisms evolved from unusually fast rates. under such model, amount change along particular lineage essentially independent its height, further weakens clock hypothesis. Finally, our predicts existence “living fossil” sister groups poor exhibited slow change. highly stochastic, it includes no special moments or epochs. Our results demonstrate observed can modelled without invoking mechanisms innovations unique specific times taxa, even when they non-uniform: instead could emerge fundamentally homogeneous throughout

Язык: Английский

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Mating system variation and gene expression in the male reproductive tract of Peromyscus mice

Authorea (Authorea), Год журнала: 2024, Номер unknown

Опубликована: Июнь 2, 2024

Genes involved in reproduction often evolve rapidly due to postcopulatory sexual selection (PCSS) driven by male-male competition and male-female conflict, but the impact of PCSS on gene expression has been under-explored. Further, though multiple tissues contribute male reproductive success, most studies have focused testes. To explore influence mating system variation Peromyscus tract, we generated RNAseq libraries quantified testis, seminal vesicle, epididymis, liver wild-caught monogamous P. californicus polygynandrous boylii maniculatus males tested for positive relaxed Peromyscus-expressed genes using published rodent genomes. We identified 3,627 mating-system associated differentially expressed (MS-DEGs), where shifted same direction relative californicus. Gene was strongly with behavior vesicles, 89% were MS-DEGs, including two key fluid proteins, Svs2 Pate4. Though showing evidence or differential did not overlap, 203 MS-DEGs showed selection. Additionally, fourteen under tree-wide convergent Microtus ochrogaster, a distantly related species. Changes transcript abundance sequence evolution association suggest that mice may respond altering aspects sperm motility, sperm-egg binding, copulatory plug formation.

Язык: Английский

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