A systematic review of the research progress of non-coding RNA in neuroinflammation and immune regulation in cerebral infarction/ischemia-reperfusion injury

Frontiers in Immunology, Год журнала: 2022, Номер 13

Опубликована: Окт. 7, 2022

Cerebral infarction/ischemia-reperfusion injury is currently the disease with highest mortality and disability rate of cardiovascular disease. Current studies have shown that nerve cells die ischemia several hours after ischemic stroke, which activates innate immune response in brain, promotes production neurotoxic substances such as inflammatory cytokines, chemokines, reactive oxygen species − nitrogen oxide, mediates destruction blood-brain barrier occurrence a series cascade reactions. Meanwhile, expression adhesion molecules cerebral vascular endothelial increased, polymorphonuclear neutrophils, lymphocytes mononuclear macrophages passed through entered brain tissue. These recognize antigens exposed by central nervous system activate adaptive responses, further mediate secondary neuronal damage, aggravating neurological deficits. In order to reduce above-mentioned body induces peripheral immunosuppressive responses negative feedback, increases incidence post-stroke infection. This process accompanied changes status tissue local systemic systems. A growing number implicate noncoding RNAs (ncRNAs) novel epigenetic regulatory elements dysfunction various cell subsets neurovascular unit injury. particular, recent revealed advances ncRNA biology greatly expand understanding regulation inflammation Identification aberrant patterns associated biological effects ncRNAs patients their potential biomarkers therapeutic targets for Therefore, this review systematically presents on involvement neuroimmune cascades, elucidates functions mechanisms infarction/ischemia-reperfusion-related ncRNAs, providing new opportunities discovery targeted therapy. Furthermore, introduces clustered regularly interspaced short palindromic repeats (CRISPR)-Display possible transformative tool studying lncRNAs. future, expected be used target diagnosing injury, judging its prognosis treatment, thereby significantly improving patients.

Язык: Английский

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The multifaceted actions of the lncRNA H19 in cardiovascular biology and diseases

Clinical Science, Год журнала: 2022, Номер 136(15), С. 1157 - 1178

Опубликована: Авг. 1, 2022

Abstract Cardiovascular diseases are the leading cause of death and debility worldwide. Various molecular mechanisms have been studied to better understand development progression cardiovascular pathologies with hope eradicate these diseases. With advancement sequencing technology, it is revealed that majority our genome non-coding. A growing body literature demonstrates critical role long non-coding RNAs (lncRNAs) as epigenetic regulators gene expression. LncRNAs can regulate cellular biological processes through various distinct mechanisms. The abundance lncRNAs in system indicates their significance physiology pathology. LncRNA H19, particular, a highly evolutionarily conserved lncRNA enriched cardiac vascular tissue, underlining its importance maintaining homeostasis system. In this review, we discuss versatile function H19 types We highlight current on demonstrate how dysregulation induces pathophysiology.

Язык: Английский

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Long non-coding RNA H19: Physiological functions and involvements in central nervous system disorders

Neurochemistry International, Год журнала: 2021, Номер 148, С. 105072 - 105072

Опубликована: Май 28, 2021

Central nervous system (CNS) disorders are some of the most complex and challenging diseases because intricate structure functions CNS. Long non-coding RNA (LncRNA) H19, which had been mistaken for "transcription noise" previously, has now found to be closely related development homeostasis Several recent studies indicate that it plays an important role in pathogenesis, treatment, even prognosis CNS disorders. LncRNA H19 is correlated with susceptibility various such as intracranial aneurysms, ischemic stroke, glioma, neuroblastoma. Moreover, participates pathogenesis by regulating transcription, translation, signaling pathways, suggesting a promising biomarker therapeutic target these This article reviews mechanisms lncRNA disorders, including cerebral ischemia, hemorrhage, pituitary adenoma, neuroblastoma, Parkinson's disease, Alzheimer's traumatic spinal cord injury, neuropathic pain, temporal lobe epilepsy, provide theoretical basis further research on

Язык: Английский

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Epigenetic mechanisms and potential therapeutic targets in stroke

Journal of Cerebral Blood Flow & Metabolism, Год журнала: 2022, Номер 42(11), С. 2000 - 2016

Опубликована: Июль 19, 2022

Accumulating evidence indicates a central role for epigenetic modifications in the progression of stroke pathology. These mechanisms are involved complex and dynamic processes that modulate post-stroke gene expression, cellular injury response, motor function, cognitive ability. Despite decades research, continues to be classified as leading cause death disability worldwide with limited clinical interventions. Thus, technological advances field epigenetics may provide innovative targets develop new therapies. This review presents on impact epigenomic readers, writers, erasers both ischemic hemorrhagic pathophysiology. We specifically explore DNA methylation, hydroxymethylation, histone modifications, regulation by long non-coding RNAs modulating expression functional outcome after stroke. Furthermore, we highlight promising pharmacological approaches biomarkers relation translational therapeutic applications.

Язык: Английский

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Long Non-Coding RNA-Mediated Competing Endogenous RNA Networks in Ischemic Stroke: Molecular Mechanisms, Therapeutic Implications, and Challenges

Frontiers in Pharmacology, Год журнала: 2021, Номер 12

Опубликована: Ноя. 17, 2021

Ischemic stroke (IS) is a disease that characterized by high mortality and disability. Recent studies have shown LncRNA-mediated competing endogenous RNA (ceRNA) networks play roles in the occurrence development of cerebral I/R injury regulating different signaling pathways. However, no systematic analysis ceRNA mechanisms IS has been reported. In this review, we discuss molecular under injury. The expression levels LncRNAs, microRNAs (miRNAs), messenger RNAs (mRNAs) their effects four major cell types neurovascular unit (NVU) are also involved. We further summarize LncRNAs as biomarkers therapeutic targets. Finally, analyze advantages limitations using therapeutics for IS.

Язык: Английский

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Effects and early diagnostic value of lncRNA H19 on sepsis‑induced acute lung injury

Experimental and Therapeutic Medicine, Год журнала: 2022, Номер 23(4)

Опубликована: Фев. 14, 2022

Sepsis is an immune disease induced by microbial invasion. The molecular mechanism and value of long non-coding H19 (lncRNA H19) in sepsis remain largely unknown. present study aimed to investigate the effects early diagnostic lncRNA on sepsis-induced acute lung injury (ALI). Serum samples from 85 septic patients 76 healthy individuals were collected, expression was assessed quantitative polymerase chain reaction (qPCR). Sprague-Dawley (SD) rats subjected cecal ligation puncture (CLP) order construct models ALI. A total 18 successfully modeled randomly allocated into H19-ad group a model group, another 9 SD same batch selected as control group. serum tissue collected. quantified qPCR, levels tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-17, caspase-3, caspase-9, B-cell lymphoma-2 (Bcl-2), BCL2-associated X (Bax) measured western blotting. receiver operating characteristic (ROC) curve employed assess for patients. downregulated sepsis. Upregulation inhibited TNF-α, IL-6, caspase-9 Bax increased Bcl-2. AUC diagnosis 0.8197 (95% CI, 0.77 0.91). alleviated ALI inhibiting pulmonary apoptosis inflammation, serving biochemical marker therapeutic target

Язык: Английский

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Expression, Functional Polymorphism, and Diagnostic Values of MIAT rs2331291 and H19 rs217727 Long Non-Coding RNAs in Cerebral Ischemic Stroke Egyptian Patients

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(2), С. 842 - 842

Опубликована: Янв. 10, 2024

Cerebral ischemic stroke (CIS) is a severe cerebral vascular event. This research aimed to evaluate the role of single-nucleotide polymorphisms (SNPs) lncRNAs MIAT rs2331291 and H19 rs217727 epigenetic methylation in expression patterns serum lncRNA CIS Egyptian patients. It included 80 cases 40 healthy subjects. Serum levels decreased, whereas increased among compared controls. For rs2331291, there were significant differences genotypic allelic frequencies between subjects at p = 0.02 0.0001, respectively. Our findings illustrated significantly T/T genotype frequency hypertensive non-hypertensive 0.004. However, gene C/C was not higher than CIS. The promoter patients level positively correlated with Receiver operating characteristics (ROC) analysis revealed that have high diagnostic potential for distinguishing from ones. In conclusion, MIAT-rs2331291 polymorphism might serve as novel indicator

Язык: Английский

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Long noncoding RNA H19 knockdown promotes angiogenesis via IMP2 after ischemic stroke

CNS Neuroscience & Therapeutics, Год журнала: 2024, Номер 30(8)

Опубликована: Авг. 1, 2024

This study aimed to explore the effects of long noncoding RNA (lncRNA) H19 knockdown on angiogenesis and blood-brain barrier (BBB) integrity following cerebral ischemia/reperfusion (I/R) elucidate their underlying regulatory mechanisms.

Язык: Английский

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Expression and diagnostic values of MIAT, H19, and NRON long non-coding RNAs in multiple sclerosis patients

Egyptian Journal of Medical Human Genetics, Год журнала: 2022, Номер 23(1)

Опубликована: Март 7, 2022

Abstract Background Multiple sclerosis (MS) is a chronic inflammatory disease. Various long non-coding RNAs (lncRNAs) appear to have an important role in the pathophysiology of MS. This study aimed at evaluating expression levels lncRNAs, MIAT , H19, and NRON peripheral blood MS cases healthy control group. We collected samples 95 (76 relapsing–remitting (RR) 19 secondary progressive (SP) MS) controls. used quantitative real-time PCR for evaluation gene expression. The correlation between with clinical parameters was analyzed by multiple linear regression model. Receiver operating characteristic (ROC) curve analysis carried out detect diagnostic potential lncRNAs according area under (AUC). Results MIAT, H19 were significantly increased RRMS SPMS subgroups compared found that higher RRMS. Patients had greater level average patients. females relative male Based on (AUC) values, best performance differentiation patients from controls (AUC = 0.95, P < 0.0001). A combination could be useful differentiating 93.6% sensitivity, 98.9% specificity, power 0.96 ( Conclusions biomarkers diagnosis.

Язык: Английский

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The Common LncRNAs of Neuroinflammation-Related Diseases

Molecular Pharmacology, Год журнала: 2022, Номер 103(3), С. 113 - 131

Опубликована: Дек. 1, 2022

Spatio-temporal specific long noncoding RNAs (lncRNAs) play important regulatory roles not only in the growth and development of brain but also occurrence neurologic diseases. Generally, diseases is accompanied by neuroinflammation. Elucidation mechanisms lncRNAs on neuroinflammation helpful for clinical treatment This paper focuses recent findings effect neuroinflammatory selects 10 that have been intensively studied to analyze their mechanism action. The status as drug targets reviewed. SIGNIFICANCE STATEMENT: Gene therapies such clustered regularly interspaced short palindrome repeats technology, antisense RNA RNAi technology are gradually applied treatment, based a large number basic research investigations. regulation neuroinflammation, elucidates beneficial or harmful effects neurosystemic diseases, provides theoretical bases targets.

Язык: Английский

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