Exploring breast tissue microbial composition and the association with breast cancer risk factors

Breast Cancer Research, Год журнала: 2023, Номер 25(1)

Опубликована: Июль 10, 2023

Abstract Background Microbial dysbiosis has emerged as an important element in the development and progression of various cancers, including breast cancer. However, microbial composition from healthy individuals, even relative to risk developing cancer, remains unclear. Here, we performed a comprehensive analysis microbiota normal tissue, which was analyzed relation tumor adjacent tissue. Methods The study cohorts included 403 cancer-free women (who donated tissue cores) 76 cancer patients and/or samples). Microbiome profiling obtained by sequencing nine hypervariable regions 16S rRNA gene (V1V2, V2V3, V3V4, V4V5, V5V7, V7V9). Transcriptome also on 190 samples. Breast score assessed using Tyrer-Cuzick model. Results V1V2 amplicon resulted more suitable for microbiome identified Lactobacillaceae ( Firmicutes phylum), Acetobacterraceae , Xanthomonadaceae (both Proteobacteria phylum) most abundant families breast. Ralstonia both tumors histologically tissues malignant tumors. We conducted correlation between known factors. Abundances bacterial taxa Acetotobacter aceti Lactobacillus vini, paracase i, Xanthonomas sp. were associated with age p < 0.0001), racial background parity 0.0001). Finally, transcriptome showed enrichment metabolism- immune-related genes vini paracasei whereas presence linked dysregulation involved carbohydrate metabolic pathway. Conclusions This defines features thus providing basis understand cancer-related dysbiosis. Moreover, findings reveal that lifestyle factors can significantly affect composition.

Язык: Английский

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Modulating Microbiota as a New Strategy for Breast Cancer Prevention and Treatment

Microorganisms, Год журнала: 2022, Номер 10(9), С. 1727 - 1727

Опубликована: Авг. 27, 2022

Breast cancer (BC) is the most common in women United States. There has been an increasing incidence and decreasing mortality rate of BC cases over past several decades. Many risk factors are associated with BC, such as diet, aging, personal family history, obesity, some environmental factors. Recent studies have shown that healthy individuals patients different microbiota composition, indicating microbiome a new factor for BC. Gut breast alterations prognosis. This review will evaluate altered populations gut, tissue, milk patients, well mechanisms interactions between modulation Probiotics prebiotics commercially available dietary supplements to alleviate side-effects therapies. They also shape population human gut microbiome. evaluates novel means modulating by nutritional treatment probiotics emerging promising strategies prevention The mechanistic role probiotic partially depend on estrogen metabolism, systematic immune regulation, epigenetics regulation.

Язык: Английский

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Fusobacterium nucleatum: a novel immune modulator in breast cancer?

Expert Reviews in Molecular Medicine, Год журнала: 2023, Номер 25

Опубликована: Янв. 1, 2023

Abstract Breast cancer was the most commonly diagnosed worldwide in 2020. Greater understanding of factors which promote tumour progression, metastatic development and therapeutic resistance is needed. In recent years, a distinct microbiome has been detected breast, site previously thought to be sterile. Here, we review clinical molecular relevance oral anaerobic bacterium Fusobacterium nucleatum breast cancer. F. enriched tissue compared with matched healthy shown mammary growth progression mouse models. Current literature suggests that modulates immune escape inflammation within microenvironment, two well-defined hallmarks Furthermore, microbiome, specifically, affect patient response therapy including checkpoint inhibitors. These findings highlight areas future research needed better understand influence treatment

Язык: Английский

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Mendelian randomization analysis revealed a gut microbiota–mammary axis in breast cancer

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Авг. 23, 2023

Observational epidemiological studies suggested an association between the gut microbiota and breast cancer, but it remains unclear whether causally influences risk of cancer. We employed two-sample Mendelian randomization (MR) analysis to investigate this association.We used summary statistics microbiome from a genome-wide study (GWAS) 18,340 individuals in MiBioGen study. GWAS for overall cancer hormone receptor subtype-specific analyses were obtained UK Biobank FinnGen databases, totaling 400,000 individuals. The inverse variance-weighted (IVW) MR method was examine causal relationship its subtypes. Sensitivity conducted using maximum likelihood, MR-Egger, pleiotropic residual sums outliers methods.The IVW estimates indicated that increased abundance Genus_Sellimonas is associated with ER+ [odds ratio (OR) = 1.09, p 1.72E-04, false discovery rate (FDR) 0.02], whereas Genus_Adlercreutzia protective against (OR 0.88, 6.62E-04, FDR 0.04). For Her2+ Genus_Ruminococcus2 decreased 0.77, 4.91E-04, 0.04), Genus_Erysipelatoclostridium 1.25, 6.58E-04, No evidence heterogeneity or horizontal pleiotropy found.Our revealed microbiota-mammary axis, providing important data supporting potential use as candidate target prevention, diagnosis, treatment.

Язык: Английский

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The microbiome: a link between obesity and breast cancer risk

Frontiers in Microbiomes, Год журнала: 2024, Номер 3

Опубликована: Май 3, 2024

Globally, breast cancer is the leading cause of incidence and mortality among all female cancers. Hereditary factors only account for 5-10% cancers, highlighting importance non-hereditary factors, such as obesity. The increasing prevalence obesity underscores need to understand its contribution risk. Multiple mechanisms may mediate pro-carcinogenic effects obesity, including altered adipokine levels, local systemic inflammation, disruption insulin insulin-like growth factor signaling, increased estrogen alterations microbiome. In this review, we focus on link between gut microbiome risk in context First, discuss how influences Next, describe effect carcinogenesis, underlying molecular mechanisms. Finally, review preclinical data interactions host bacteria, current challenges study obesity-microbiome connection, future perspectives field.

Язык: Английский

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Population-specific differences in the human microbiome: Factors defining the diversity

Gene, Год журнала: 2024, Номер 933, С. 148923 - 148923

Опубликована: Сен. 6, 2024

Язык: Английский

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A novel combinatorial approach using sulforaphane- and withaferin A-rich extracts for prevention of estrogen receptor-negative breast cancer through epigenetic and gut microbial mechanisms

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Май 27, 2024

Abstract Estrogen receptor-negative [ER(−)] mammary cancer is the most aggressive type of breast (BC) with higher rate metastasis and recurrence. In recent years, dietary prevention BC epigenetically active phytochemicals has received increased attention due to its feasibility, effectiveness, ease implementation. this regard, combinatorial phytochemical intervention enables more efficacious inhibition by simultaneously targeting multiple tumorigenic pathways. We, therefore, focused on investigation effect sulforaphane (SFN)-rich broccoli sprouts (BSp) withaferin A (WA)-rich Ashwagandha (Ash) combination in estrogen using transgenic mice. Our results indicated that BSp + Ash treatment significantly reduced tumor incidence growth (~ 75%) as well delayed 21%) latency when compared control was statistically effective suppressing single or intervention. At molecular level, upregulated suppressors (p53, p57) along apoptosis associated proteins (BAX, PUMA) BAX:BCL-2 ratio. Furthermore, our result an expressional decline epigenetic machinery HDAC1 DNMT3A tissue because treatment. Interestingly, we have reported synergistic interactions between impacted both phenotype expression RNA-seq analysis also demonstrated a transcriptome-wide reshuffling genes cell-signaling pathways, transcription factor activity regulations combined administration. addition, discovered alteration gut microbial composition change Overall, supplementation can prevent ER(−) through enhanced suppression, induction gene possibly influencing cell signaling regulation reshaping microbiota.

Язык: Английский

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Guideline for designing microbiome studies in neoplastic diseases

GeroScience, Год журнала: 2024, Номер 46(5), С. 4037 - 4057

Опубликована: Июнь 26, 2024

Oncobiosis has emerged as a key contributor to the development, and modulator of treatment efficacy cancer. Hereby, we review modalities through which oncobiome can support progression tumors, emerging therapeutic opportunities they present. The highlights inherent challenges limitations faced in sampling accurately characterizing oncobiome. Additionally, underscores critical need for standardization microbial analysis techniques consistent reporting microbiome data. We provide suggested metadata set that should accompany datasets from oncological settings so studies remain comparable decipherable.

Язык: Английский

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Specific microbiome patterns and their association with breast cancer: the intestinal microbiota as a potential biomarker and therapeutic strategy

Clinical & Translational Oncology, Год журнала: 2024, Номер unknown

Опубликована: Июнь 18, 2024

Abstract Breast cancer (BC) is one of the most diagnosed cancers in women. Based on histological characteristics, they are classified as non-invasive, or situ (tumors located within milk ducts lobules) and invasive. BC may develop from carcinomas over time. Determining prognosis predicting response to treatment essential tools manage this disease reduce its incidence mortality, well promote personalized therapy for patients. However, half cases not associated with known risk factors. In addition, some patients resistance relapse. Therefore, it necessary identify new biomarkers strategies that improve existing therapies. regard, role microbiome being researched could play a carcinogenesis efficacy This review aims describe specific patterns BC. For this, literature search was carried out PubMed database using MeSH terms “Breast Neoplasms” “Gastrointestinal Microbiome”, including 29 publications. Most studies have focused characterizing gut breast tissue Likewise, animal models vitro investigated impact microbiota (GM) treatments effects tumor cells were included. results included articles, be an imbalance GM. varied depending molecular type, stage grade cancer, menopause, menarche, body mass index, physical activity. microbial profile identified biomarker. On other hand, suggest GM influence microorganisms bacterial metabolites therapies development.

Язык: Английский

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The Link Between the Microbiota and HER2+ Breast Cancer: The New Challenge of Precision Medicine

Frontiers in Oncology, Год журнала: 2022, Номер 12

Опубликована: Июль 13, 2022

The microbiota is emerging as a key player in cancer due to its involvement several host physiological functions, including digestion, development of the immune system, and modulation endocrine function. Moreover, participation efficacy anticancer treatments has been well described. For instance, breast (BC) progression gained ground past years. In this review, we report discuss new findings on impact gut BC, focusing HER2+ BC subtype, possibility defining microbial signatures that are associated with disease aggressiveness, treatment response, therapy toxicity. We also novel insights into mechanisms through which microorganism-host interactions occur editing prevention optimization BC.

Язык: Английский

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