Exosome derived from tumor-associated macrophages: biogenesis, functions, and therapeutic implications in human cancers

Biomarker Research, Год журнала: 2023, Номер 11(1)

Опубликована: Ноя. 19, 2023

Abstract Tumor-associated macrophages (TAMs), one of the most abundant immune cell types in tumor microenvironment (TME), account for approximately 50% local hematopoietic cells. TAMs play an important role tumorigenesis and development through crosstalk between various cells cytokines TME. Exosomes are small extracellular vesicles with a diameter 50–150 nm, that can transfer biological information (e.g., proteins, nucleic acids, lipids) from secretory to recipient circulatory system, thereby influencing progression human diseases, including cancer. Recent studies have suggested TAMs-derived exosomes crucial roles malignant proliferation, invasion, metastasis, angiogenesis, responses, drug resistance, metabolic reprogramming. potential be targeted therapy. In addition, abnormal expression non-coding RNAs proteins is closely related clinicopathological features patients cancer, these expected become new liquid biopsy markers early diagnosis, prognosis, monitoring tumors. this review, we explored provide diagnostic biomarkers therapeutic targets cancer prevention.

Язык: Английский

---

Exosomes-regulated lipid metabolism in tumorigenesis and cancer progression

Cytokine & Growth Factor Reviews, Год журнала: 2023, Номер 73, С. 27 - 39

Опубликована: Май 24, 2023

Язык: Английский

---

Circulating exosomal immuno-oncological checkpoints and cytokines are potential biomarkers to monitor tumor response to anti-PD-1/PD-L1 therapy in non-small cell lung cancer patients

Frontiers in Immunology, Год журнала: 2023, Номер 13

Опубликована: Янв. 18, 2023

Immune checkpoint inhibitors (ICIs) including anti-PD-1 and anti-PD-L1 antibodies, have significantly changed the treatment outcomes of NSCLC patients with better overall survival. However, 15-40% still fail to respond ICIs therapy. Identification biomarkers associated responses are mandated in order increase efficacy such In this study we evaluated 27 serum-derived exosomal immuno-oncological proteins 44 cytokines/chemokines before after therapy 17 identify surrogate for treatment/monitoring patient stratification maximum therapeutic benefit. We first confirmed identity isolated exosomes their specific markers (CD63, CD81, HSP70 CD91). demonstrated that baseline concentration exosomal-PD-L1 (p<0.0001), exosomal-PD-L2 (p=0.0413) exosomal-PD-1 (p=0.0131) from were higher than soluble-free forms. Furthermore, was present all (100%), while only 71% expressed tissue PD-L1. This indicates is a more reliable diagnostic biomarker. Interestingly, expression (p=0.0193) PD-L1-negative compared PD-L1-positive patients. also shown pre-ICIs treated counterparts (CD152, p=0.0008; CD80, p=0.0182; IDO, p=0.0443; Arginase, p<0.0001; Nectin-2, NT5E, Siglec-7, Siglec-9, p=0.0335; CD28, p=0.0092; GITR, MICA, p<0.0001). Finally, changes levels proteins/cytokines correlation tumor response assessed. There significant downregulation PD-L1 (p=0.0156), E-Cadherin (p=0.0312), ULBP1 ULBP3 (p=0.0391), MICA MICB (p=0.0469), Siglec7 (p=0.0078) upregulation PD-1 (p=0.0156) IFN- γ responding Non-responding showed (p=0.0078). responding-patients without liver-metastasis significant-upregulation (p=0.0070), (p=0.0137) Siglec-7 (p=0.0037). had significant-downregulation (p=0.0317) brain-metastasis significant-upregulation/downregulation (p=0.0262/0.0286) pulmonary-metastasis. time potential monitor can predict clinical

Язык: Английский

---

The Tricky Connection between Extracellular Vesicles and Mitochondria in Inflammatory-Related Diseases

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(9), С. 8181 - 8181

Опубликована: Май 3, 2023

Mitochondria are organelles present in almost all eukaryotic cells, where they represent the main site of energy production. involved several important cell processes, such as calcium homeostasis, OXPHOS, autophagy, and apoptosis. Moreover, play a pivotal role also inflammation through inter-organelle inter-cellular communications, mediated by release mitochondrial damage-associated molecular patterns (mtDAMPs). It is currently well-documented that addition to traditional endocrine paracrine communication, cells converse via extracellular vesicles (EVs). These small membrane-bound particles released from milieu under physio-pathological conditions. Importantly, EVs have gained much attention for their crucial translating inflammatory signals into recipient cells. cargo includes plasma membrane endosomal proteins, but contain material other cellular compartments, including mitochondria. Studies shown may transport portions, and/or mtDAMPs modulate metabolic responses Overall, relationship between mitochondria an active area research, although further studies needed fully understand mechanisms how be targeted therapeutic purposes. Here, we reported discussed latest focused on this fascinating recent discussing tricky connection inflammatory-related diseases.

Язык: Английский

---

Extracellular vesicles as mediators of cell-cell communication in ovarian cancer and beyond – A lipids focus

Cytokine & Growth Factor Reviews, Год журнала: 2023, Номер 73, С. 52 - 68

Опубликована: Июль 1, 2023

Extracellular vesicles (EVs) are messengers that carry information in the form of proteins, lipids, and nucleic acids not only essential for intercellular communication but also play a critical role progression various pathologies, including ovarian cancer. There has been recent substantial research characterising EV cargo, specifically, lipid profile EVs. Lipids involved formation cargo sorting EVs, their release cellular uptake. Numerous lipidomic studies demonstrated enrichment specific classes lipids EVs derived from cancer cells suggesting associated can potentially be employed as minimally invasive biomarkers early diagnosis malignancies, In this review, we aim to provide general overview heterogeneity EV, biogenesis, content, function focussing on

Язык: Английский

---

Atherosclerosis and Its Related Laboratory Biomarkers

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(21), С. 15546 - 15546

Опубликована: Окт. 24, 2023

Atherosclerosis constitutes a persistent inflammatory ailment, serving as the predominant underlying condition for coronary artery disease (CAD), peripheral (PAD), and cerebrovascular disease. The progressive buildup of plaques within walls medium- large-caliber arteries characterizes atherosclerotic process. This accumulation results in significant narrowing that impedes blood flow, leading to critical tissue oxygen deficiency. Spontaneous blockage thrombotic vessels can precipitate stroke myocardial infarction, which are complications representing primary global causes mortality. Present-day models predicting cardiovascular risk incorporate conventional factors gauge likelihood events over ten-year span. In recent times, researchers have identified serum biomarkers associated with an elevated events. Many these biomarkers, whether used individually or combination, been integrated into prediction assess their inclusion enhances predictive accuracy. this review, we conducted comprehensive analysis most recently published literature concerning atherosclerosis. We explored potential utility incorporating markers guiding clinical decisions.

Язык: Английский

---

Environmental pollutants and exosomes: A new paradigm in environmental health and disease

The Science of The Total Environment, Год журнала: 2024, Номер 925, С. 171774 - 171774

Опубликована: Март 18, 2024

Язык: Английский

---

Role and therapeutic perspectives of extracellular vesicles derived from liver and adipose tissue in metabolic dysfunction-associated steatotic liver disease

Artificial Cells Nanomedicine and Biotechnology, Год журнала: 2024, Номер 52(1), С. 355 - 369

Опубликована: Июнь 4, 2024

The global epidemic of metabolic diseases has led to the emergence dysfunction-associated steatotic liver disease (MASLD) and steatohepatitis (MASH), which pose a significant threat human health. Despite recent advances in research on pathogenesis treatment MASLD/MASH, there is still lack more effective targeted therapies. Extracellular vesicles (EVs) discovered wide range tissues body fluids encapsulate different activated biomolecules mediate intercellular communication. Recent studies have shown that EVs derived from adipose tissue (AT) play vital roles MASLD/MASH therapeutics, depending their sources intervention types. Besides, adipose-derived stem cell (ADSC)-derived appear be mitigating MASLD/MASH. This review presents an overview definition, extraction strategies, characterisation EVs, with particular focus biogenesis release exosomes. It also reviews effects potential molecular mechanisms liver- AT-derived emphasises contribution clinical therapeutic ADSC-derived EVs. Furthermore, future perspective EV therapy setting discussed.

Язык: Английский

---

Engineered and Mimicked Extracellular Nanovesicles for Therapeutic Delivery

Nanomaterials, Год журнала: 2024, Номер 14(7), С. 639 - 639

Опубликована: Апрель 6, 2024

Exosomes are spherical extracellular nanovesicles with an endosomal origin and unilamellar lipid-bilayer structure sizes ranging from 30 to 100 nm. They contain a large range of proteins, lipids, nucleic acid species, depending on the state vesicle (EV)-secreting cell. EVs’ function is encapsulate part EV-producing cell content, transport it through biological fluids targeted recipient, deliver their cargos specifically within aimed recipient cells. Therefore, exosomes considered be potential drug-delivery systems that can stably cargo into Various cell-derived produced for medical issues, but use therapeutic purposes still faces several problems. Some these difficulties avoided by resorting hemisynthetic approaches. We highlight here uses alternative exosome-mimes involving cell-membrane coatings artificial nanocarriers or hybridization between liposomes. also detail drug-loading strategies deployed make them drug-carrier summarize ongoing clinical trials exosome-like structures. Finally, we open questions before considering disposals confident delivery.

Язык: Английский

---

Engineering Exosomes for Therapeutic Applications: Decoding Biogenesis, Content Modification, and Cargo Loading Strategies

International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 7137 - 7164

Опубликована: Июль 1, 2024

Abstract: Exosomes emerge from endosomal invagination and range in size 30 to 200 nm. contain diverse proteins, lipids, nucleic acids, which can indicate the state of various physiological pathological processes. Studies have revealed remarkable clinical potential exosomes diagnosing prognosing multiple diseases, including cancer, cardiovascular disorders, neurodegenerative conditions. also be engineered deliver their cargo a specific target. However, further advancements are imperative optimize exosomes' diagnostic therapeutic capabilities for practical implementation settings. This review highlights applications, emphasizing engineering through simple incubation, biological, click chemistry techniques. Additionally, loading agents onto exosomes, utilizing passive active strategies, exploring hybrid artificial discussed. Keywords: exosome, drug delivery, diagnostics, therapeutics

Язык: Английский

---