Targeted Therapy for Inflammatory Diseases with Mesenchymal Stem Cells and Their Derived Exosomes: From Basic to Clinics

International Journal of Nanomedicine, Год журнала: 2022, Номер Volume 17, С. 1757 - 1781

Опубликована: Апрель 1, 2022

Abstract: Inflammation is a beneficial and physiological process, but there are number of inflammatory diseases which have detrimental effects on the body. In addition, drugs used to treat inflammation toxic side when over long period time. Mesenchymal stem cells (MSCs) pluripotent that can be isolated from variety tissues differentiate into diverse cell types under appropriate conditions. They also exhibit noteworthy anti-inflammatory properties, providing new options for treatment diseases. The therapeutic potential MSCs currently being investigated various diseases, such as kidney injury, lung osteoarthritis (OA), rheumatoid arthritis (RA), bowel disease (IBD). perform multiple functions, including immunomodulation, homing, differentiation, enable damaged form balanced regenerative microenvironment severe accumulated evidence indicates exosomes extracellular vesicles (MSC-Exos) play an extraordinary role, mainly by transferring their components recipient cells. this review, we summarize mechanism clinical trials MSC-Exos in detail, with view contributing Keywords: mesenchymal cells, exosome, clinic data, drug delivery, targeted therapy

Язык: Английский

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Clinical Translation of Extracellular Vesicles

Advanced Healthcare Materials, Год журнала: 2023, Номер 12(28)

Опубликована: Июль 8, 2023

Extracellular vesicles (EVs) occur in a variety of bodily fluids and have gained recent attraction as natural materials due to their bioactive surfaces, internal cargo, role intercellular communication. EVs contain various biomolecules, including surface cytoplasmic proteins; nucleic acids that are often representative the originating cells. can transfer content other cells, process is thought be important for several biological processes, immune responses, oncogenesis, angiogenesis. An increased understanding underlying mechanisms EV biogenesis, composition, function has led an exponential increase preclinical clinical assessment biomedical applications, such diagnostics drug delivery. Bacterium-derived vaccines been use decades few EV-based diagnostic assays regulated under Clinical Laboratory Improvement Amendments approved single laboratories. Though, products yet receive widespread approval from national regulatory agencies United States Food Drug Administration (USFDA) European Medicine Agency (EMA), many late-stage trials. This perspective sheds light on unique characteristics EVs, highlighting current trends, emerging challenges future perspectives use.

Язык: Английский

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Curcumin-Based Nanomedicines in the Treatment of Inflammatory and Immunomodulated Diseases: An Evidence-Based Comprehensive Review

Pharmaceutics, Год журнала: 2023, Номер 15(1), С. 229 - 229

Опубликована: Янв. 10, 2023

Curcumin (CUR) is a polyphenol extracted from the rhizome of Curcuma longa that possesses potent anti-inflammatory and antioxidant potential. Despite CUR’s numerous beneficial effects on human health, it has limitations, such as poor absorption. Nano-based drug delivery systems have recently been applied to improve solubility bioavailability potentialize its health effects. This review investigated different CUR-based nanomedicines inflammatory immunomodulated diseases. PUBMED, EMBASE, COCHRANE, GOOGLE SCHOLAR databases were searched, Scale for Assessment Narrative Review Articles (SANRA) was used quality assessment PRISMA guidelines. Overall, 66 studies included comprising atherosclerosis, rheumatoid arthritis (RA), Alzheimer’s disease (AD), Parkinson’s (PD), multiple sclerosis (MS), Huntington’s (HD), bowel diseases (IBD), psoriasis, liver fibrosis, epilepsy, COVID-19. The available scientific show there are many known nanoformulations with curcumin. They can be found in nanosuspensions, nanoparticles, nanoemulsions, solid lipid particles, nanocapsules, nanospheres, liposomes. These formulations CUR effectively adjuvants several immune-mediated atheroma plaque formation, RA, dementia, AD, PD, MS, IBD, COVID-19, anti-fibrotic fibrotic disease.

Язык: Английский

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Understanding exosomes: Part 2—Emerging leaders in regenerative medicine

Periodontology 2000, Год журнала: 2024, Номер 94(1), С. 257 - 414

Опубликована: Фев. 1, 2024

Abstract Exosomes are the smallest subset of extracellular signaling vesicles secreted by most cells with ability to communicate other tissues and cell types over long distances. Their use in regenerative medicine has gained tremendous momentum recently due their be utilized as therapeutic options for a wide array diseases/conditions. Over 5000 publications currently being published yearly on this topic, number is only expected dramatically increase novel strategies continue developed. Today exosomes have been applied numerous contexts including neurodegenerative disorders (Alzheimer's disease, central nervous system, depression, multiple sclerosis, Parkinson's post‐traumatic stress disorders, traumatic brain injury, peripheral nerve injury), damaged organs (heart, kidney, liver, stroke, myocardial infarctions, ovaries), degenerative processes (atherosclerosis, diabetes, hematology musculoskeletal degeneration, osteoradionecrosis, respiratory disease), infectious diseases (COVID‐19, hepatitis), procedures (antiaging, bone regeneration, cartilage/joint osteoarthritis, cutaneous wounds, dental dermatology/skin erectile dysfunction, hair regrowth, intervertebral disc repair, spinal cord vascular regeneration), cancer therapy (breast, colorectal, gastric osteosarcomas), immune function (allergy, autoimmune regulation, inflammatory diseases, lupus, rheumatoid arthritis). This scoping review first its kind aimed at summarizing extensive potential broad range disorders.

Язык: Английский

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Harnessing Tissue-derived Extracellular Vesicles for Osteoarthritis Theranostics

Theranostics, Год журнала: 2021, Номер 12(1), С. 207 - 231

Опубликована: Ноя. 10, 2021

Osteoarthritis (OA) is a prevalent chronic whole-joint disease characterized by low-grade systemic inflammation, degeneration of joint-related tissues such as articular cartilage, and alteration bone structures that can eventually lead to disability. Emerging evidence has indicated synovium or cartilage-secreted extracellular vesicles (EVs) contribute OA pathogenesis physiology, including transporting enhancing the production inflammatory mediators cartilage degrading proteinases. Bioactive components EVs are known play role in include microRNA, long non-coding RNA, proteins. Thus, tissues-derived be used combination with advanced nanomaterial-based biosensors for diagnostic assessment progression. Alternatively, mesenchymal stem cell- platelet-rich plasma-derived (MSC-EVs PRP-EVs) have high therapeutic value treating OA, suppressing immune microenvironment, which often enriched pro-inflammatory cells cytokines reduce chondrocytes apoptosis. Moreover, those modified incorporated into biomaterials enhanced targeting prolonged retention treat effectively. In this review, we explore recently reported OA-related pathological biomarkers from joint tissue-derived discuss possibility current detecting EV-related biomarkers. We summarize applications MSC-EVs PRP-EVs their limitations regeneration alleviating symptoms. Additionally, identify strategies, engineered applying increase efficacy EV-based therapies. Finally, provide our perspective on future diagnosis potential treatment.

Язык: Английский

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Breakthrough of extracellular vesicles in pathogenesis, diagnosis and treatment of osteoarthritis

Bioactive Materials, Год журнала: 2022, Номер 22, С. 423 - 452

Опубликована: Окт. 20, 2022

Osteoarthritis (OA) is a highly prevalent whole-joint disease that causes disability and pain affects patient's quality of life. However, currently, there lack effective early diagnosis treatment. Although stem cells can promote cartilage repair treat OA, problems such as immune rejection tumorigenicity persist. Extracellular vesicles (EVs) transmit genetic information from donor mediate intercellular communication, which considered functional paracrine factor cells. Increasing evidences suggest EVs may play an essential complex role in the pathogenesis, diagnosis, treatment OA. Here, we introduced OA progression by influencing inflammation, metabolism, aging. Next, discussed blood, synovial fluid, joint-related for diagnosis. Moreover, outlined potential modified unmodified their combination with biomaterials therapy. Finally, discuss deficiencies put forward prospects challenges related to application field

Язык: Английский

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Advances in Extracellular Vesicle Nanotechnology for Precision Theranostics

Advanced Science, Год журнала: 2022, Номер 10(3)

Опубликована: Ноя. 14, 2022

Abstract Extracellular vesicles (EVs) have increasingly been recognized as important cell surrogates influencing many pathophysiological processes, including cellular homeostasis, cancer progression, neurologic disease, and infectious disease. These behaviors enable EVs broad application prospects for clinical in disease diagnosis treatment. Many studies suggest that are superior to conventional synthetic carriers terms of drug delivery circulating biomarkers early diagnosis, opening up new frontiers modern theranostics. Despite these potential, containing diverse components, such nucleic acids, proteins, metabolites highly heterogeneous small size. The limitation preparatory, engineering analytical technologies poses technical barriers translation. This article aims at present a critical overview emerging field biomedical applications challenges involved their clinic translations. current methods isolation identification discussed. Additionally, strategies developed enhance scalable production improved cargo loading well tumor targeting presented. potential EVs, particularly different origins the next generation diagnostic treatment platforms, clarified.

Язык: Английский

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Knee Osteoarthritis Therapy: Recent Advances in Intra-Articular Drug Delivery Systems

Drug Design Development and Therapy, Год журнала: 2022, Номер Volume 16, С. 1311 - 1347

Опубликована: Май 1, 2022

Abstract: Drug delivery for osteoarthritis (OA) treatment is a continuous challenge because of their poor bioavailability and rapid clearance in joints. Intra-articular (IA) drug common strategy its therapeutic effects depend mainly on the efficacy drug-delivery system used OA therapy. Different types IA systems, such as microspheres, nanoparticles, hydrogels, have been rapidly developed over past decade to improve effects. With advancement mechanism research, new drugs targeting specific cell/signaling pathways are evolving effective critical treating OA. In this review, recent advances various systems treatment, targeted strategies, related signaling summarized analyzed based current publications. Keywords: osteoarthritis, knee, delivery, intra-articular

Язык: Английский

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Engineering of MSC-Derived Exosomes: A Promising Cell-Free Therapy for Osteoarthritis

Membranes, Год журнала: 2022, Номер 12(8), С. 739 - 739

Опубликована: Июль 28, 2022

Osteoarthritis (OA) is characterized by progressive cartilage degeneration with increasing prevalence and unsatisfactory treatment efficacy. Exosomes derived from mesenchymal stem cells play an important role in alleviating OA promoting regeneration, inhibiting synovial inflammation mediating subchondral bone remodeling without the risk of immune rejection tumorigenesis. However, low yield, weak activity, inefficient targeting ability unpredictable side effects natural exosomes have limited their clinical application. At present, various approaches been applied exosome engineering to regulate production function, such as pretreatment parental cells, drug loading, genetic surface modification. Biomaterials also proved facilitate efficient delivery enhance effectiveness. Here, we summarize current understanding biogenesis, isolation characterization exosomes, focus on large-scale preparation engineered well therapeutic potential OA, thus providing novel insights into exploring advanced MSC-derived exosome-based cell-free therapy for OA.

Язык: Английский

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Curcumin primed ADMSCs derived small extracellular vesicle exert enhanced protective effects on osteoarthritis by inhibiting oxidative stress and chondrocyte apoptosis

Journal of Nanobiotechnology, Год журнала: 2022, Номер 20(1)

Опубликована: Март 9, 2022

Osteoarthritis (OA) is a common joint disease caused by progressive articular cartilage degeneration and destruction. Currently, there are no disease-modifying agents officially approved for OA patients. In this study, curcumin was loaded into adipose tissue-derived mesenchymal stem cells (ADMSCs)-derived small extracellular vesicle (ADMSCs-sEV) to synergistically exert chondro-protective effects in vitro vivo. We found primed ADMSCs derived sEV (sEV-CUR) exhibited an enhanced protective effect compared with free ADMSCs-sEV TBHP-induced chondrocytes. Moreover, our study demonstrated sEV-CUR more effectively down-regulated oxidative stress chondrocyte apoptosis vitro. mice model, results indicated that showed improved protection, as biweekly intra-articular injection of efficaciously alleviated cartilage. Overall, findings holds great potential on the recovery loss destruction

Язык: Английский

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