Cardamonin alleviates chondrocytes inflammation and cartilage degradation of osteoarthritis by inhibiting ferroptosis via p53 pathway

Food and Chemical Toxicology, Год журнала: 2023, Номер 174, С. 113644 - 113644

Опубликована: Янв. 30, 2023

Язык: Английский

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Boosting cartilage repair with silk fibroin-DNA hydrogel-based cartilage organoid precursor

Bioactive Materials, Год журнала: 2024, Номер 35, С. 429 - 444

Опубликована: Фев. 16, 2024

Osteoarthritis (OA), a common degenerative disease, is characterized by high disability and imposes substantial economic impacts on individuals society. Current clinical treatments remain inadequate for effectively managing OA. Organoids, miniature 3D tissue structures from directed differentiation of stem or progenitor cells, mimic native organ functions. They are useful drug testing serve as active grafts repair. However, organoid construction requires extracellular matrix-like scaffolds cellular growth. Hydrogel microspheres, with tunable physical chemical properties, show promise in cartilage engineering replicating the natural microenvironment. Building prior work SF-DNA dual-network hydrogels regeneration, we developed novel RGD-SF-DNA hydrogel microsphere (RSD-MS) via microfluidic system integrating photopolymerization self-assembly techniques then modified Pep-RGDfKA. The RSD-MSs exhibited uniform size, porous surface, optimal swelling degradation properties.

Язык: Английский

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Energy metabolism as therapeutic target for aged wound repair by engineered extracellular vesicle

Science Advances, Год журнала: 2024, Номер 10(15)

Опубликована: Апрель 12, 2024

Aging skin, vulnerable to age-related defects, is poor in wound repair. Metabolic regulation accumulated senescent cells (SnCs) with aging essential for tissue homeostasis, and adequate ATP important cell activation aged Strategies metabolism intervention hold prospects therapeutic advances. Here, we found energy metabolic changes skin from patients mice. Our data show that metformin engineered EV (Met-EV) can enhance mouse repair, as well ameliorate cellular senescence restore dysfunctions. Notably, was remodeled reduced glycolysis enhanced OXPHOS after Met-EV treatment. We rescue senescence-induced mitochondria dysfunctions mitophagy suppressions, indicating the role of remodeling mitochondrial functions via production results reveal mechanism SnCs rejuvenation by suggest disturbed metabolism, be a potential target facilitating

Язык: Английский

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Ultrasound-triggered in situ gelation with ROS-controlled drug release for cartilage repair

Materials Horizons, Год журнала: 2023, Номер 10(9), С. 3507 - 3522

Опубликована: Янв. 1, 2023

Cartilage defects are usually caused by acute trauma and chronic degeneration. However, it is still a great challenge to improve the repair of articular cartilage due limited self-regeneration capacity such defects. Herein, novel ROS-responsive in situ nanocomposite hydrogel loaded with kartogenin (KGN) bone marrow-derived stem cells (BMSCs) was designed constructed via enzymatic reaction fibrinogen thrombin. Meanwhile, thioketal (TK)-based liposome synthesized load chondrogenesis-inducing factor KGN, bioenzyme thrombin an ultrasound-sensitive agent PpIX. Under ultrasound stimulation, TK-based destroyed, followed gelation Moreover, sustained release KGN realized regulating conditions. Importantly, ROS generation within microenvironment fibrin significantly promoted chondrogenic differentiation BMSCs Smad5/mTOR signalling pathway effectively improved regeneration rat defect model. Overall, ROS-controlled drug has potential for efficient repair.

Язык: Английский

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Nanomaterials‐Based Targeting of Long Non‐Coding RNAs in Cancer: A Cutting‐Edge Review of Current Trends

ChemMedChem, Год журнала: 2024, Номер 19(8)

Опубликована: Янв. 24, 2024

This review article spotlights the burgeoning potential of using nanotherapeutic strategies to target long non-coding RNAs (lncRNAs) in cancer cells. updated discourse underlines prominent role lncRNAs instigating cancer, facilitating its progression, and metastasis, validating lncRNAs' for being effective diagnostic biomarkers therapeutic targets. The manuscript offers an in-depth examination different presently employed modulate lncRNA expression function purposes. Among these strategies, Antisense Oligonucleotides (ASOs), RNA interference (RNAi) technologies, innovative clustered regularly interspaced short palindromic repeats (CRISPR)-based gene editing tools garner noteworthy mention. A significant section is dedicated nanocarriers their crucial drug delivery. These nanocarriers' efficiency targeting varied types cancers elaborated upon, importance targeted therapy. culminates by reaffirming promising prospects enhance accuracy diagnosis improve treatment efficacy. Consequently, new paths are opened more research innovation employing approaches against Thus, this comprehensive serves as a valuable resource that underscores vital various nano-strategies them treatment. Future should also focus on unraveling complex regulatory networks involving identifying fundamental functional interactions refine cancer.

Язык: Английский

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Stem cell recruitment polypeptide hydrogel microcarriers with exosome delivery for osteoarthritis treatment

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Авг. 27, 2024

With the accelerated aging tendency, osteoarthritis (OA) has become an intractable global public health challenge. Stem cells and their derivative exosome (Exo) have shown great potential in OA treatment. Research this area tends to develop functional microcarriers for stem cell Exo delivery improve therapeutic effect. Herein, we a novel system of Exo-encapsulated cell-recruitment hydrogel from liquid nitrogen-assisted microfluidic electrospray Benefited advanced droplet generation capability microfluidics mild cryogelation procedure, resultant particles show uniform size dispersion excellent biocompatibility. Moreover, acryloylated recruitment peptides SKPPGTSS are directly crosslinked within by ultraviolet irradiation, thus simplifying peptide coupling process preventing its premature release. The SKPPGTSS-modified can recruit endogenous promote cartilage repair released further enhances performance through synergistic effects. These features suggest that proposed microcarrier is promising candidate

Язык: Английский

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Engineering exosomes derived from TNF-α preconditioned IPFP-MSCs enhance both yield and therapeutic efficacy for osteoarthritis

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Сен. 11, 2024

Язык: Английский

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Logic‐Based Strategy for Spatiotemporal Release of Dual Extracellular Vesicles in Osteoarthritis Treatment

Advanced Science, Год журнала: 2024, Номер 11(26)

Опубликована: Май 5, 2024

Abstract To effectively treat osteoarthritis (OA), the existing inflammation must be reduced before cartilage damage can repaired; this cannot achieved with a single type of extracellular vesicles (EVs). Here, hydrogel complex logic‐gates function is proposed that spatiotemporally controlled release two types EVs: interleukin 10 (IL‐10) + EVs to promote M2 polarization macrophage, and SRY‐box transcription factor 9 (SOX9) increase matrix synthesis. Following dose‐of‐action screening, dual are loaded into metalloporoteinase 13 (MMP13)‐sensitive self‐assembled peptide (KM13E) polyethylene glycol diacrylate/gelatin methacryloyl‐hydrogel microspheres (PGE), respectively. These materials mixed form “microspheres‐in‐gel” KM13E@PGE system. In vitro, abruptly released IL‐10 after 3 days slowly SOX9 for more than 30 days. vivo, increased CD206 macrophage proportion in synovial tissue decreased tumor necrosis factor‐α IL‐1β levels. The aggrecan expressions tissues significantly elevated following subsidence. This performance not using anti‐inflammatory or repair therapy alone. present study provides an injectable, integrated delivery system spatiotemporal control EVs, may inspire strategies OA treatment.

Язык: Английский

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Potential and challenges of utilizing exosomes in osteoarthritis therapy (Review)

International Journal of Molecular Medicine, Год журнала: 2025, Номер 55(3)

Опубликована: Янв. 8, 2025

Exosomes are integral to the pathophysiology of osteoarthritis (OA) due their roles in mediating intercellular communication and regulating inflammatory processes. transport bioactive molecules, such as proteins, lipids nucleic acids, which can influence chondrocyte behavior joint homeostasis. Given properties regeneration ability target damaged tissues, exosomes represent a promising therapeutic avenue for OA treatment. have potential promoting cartilage repair, reducing inflammation improving overall function. However, several challenges remain, including need standardized isolation characterization methods, variability exosomal content, regulatory hurdles. The present review aims provide comprehensive overview current understanding exosome mechanisms potential, while also addressing ongoing faced translating these findings into clinical practice. By consolidating existing research, pave way future studies aimed at optimizing exosome‑based therapies effective management.

Язык: Английский

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Knee adipose tissue: from its implication in osteoarthritis to its supposed role in tissue engineering

npj Aging, Год журнала: 2025, Номер 11(1)

Опубликована: Фев. 3, 2025

Knee osteoarthritis (OA) has long been considered as a cartilage disease. However, it is now well established that defects are the ultimate OA feature only. Several studies investigating mechanisms of onset/progression have recognized infrapatellar fat pad an active OA-player but also possible source stem cells with regenerative potential for cartilage. Is really like that? cautious approach should be adopted.

Язык: Английский

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