Chondroitin Sulfate-Tyramine-Based Hydrogels for Cartilage Tissue Repair

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(4), С. 3451 - 3451

Опубликована: Фев. 9, 2023

The degradation of cartilage, due to trauma, mechanical load or diseases, results in abundant loss extracellular matrix (ECM) integrity and development osteoarthritis (OA). Chondroitin sulfate (CS) is a member the highly sulfated glycosaminoglycans (GAGs) primary component cartilage tissue ECM. In this study, we aimed investigate effect on chondrogenic differentiation bone marrow mesenchymal stem cells (BM-MCSs) encapsulated into CS-tyramine-gelatin (CS-Tyr/Gel) hydrogel order evaluate suitability composite for OA regeneration studies vitro. CS-Tyr/Gel/BM-MSCs showed excellent biointegration explants. applied mild stimulated BM-MSCs CS-Tyr/Gel (immunohistochemical collagen II staining). However, stronger had negative human explants evaluated by higher release ECM components, such as oligomeric protein (COMP) GAGs, compared not-compressed Finally, application top decreased COMP GAGs from Data suggest that can protect damaging effects external stimuli. Therefore, it be used investigation regenerative potential mechanisms under vitro with further perspectives therapeutic vivo.

Язык: Английский

---

Dental Pulp Stem Cell-Derived Exosomes Alleviate Mice Knee Osteoarthritis by Inhibiting TRPV4-Mediated Osteoclast Activation

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(5), С. 4926 - 4926

Опубликована: Март 3, 2023

Osteoarthritis (OA) is a degenerative disease that causes chronic pain and joint swelling even disables millions of patients. However, current non-surgical treatment for OA can only relieve without obvious cartilage subchondral bone repair. Mesenchymal stem cell (MSC)-secreted exosomes have promising therapeutic effects on knee OA, but the efficacy MSC-exosome therapy not well determined, mechanisms involved are still unclear. In this study, we isolated dental pulp (DPSC)-derived by ultracentrifugation determined single intra-articular injection DPSC-derived in mice model. The results showed effectively improved abnormal remodeling, inhibited occurrence sclerosis osteophytes, alleviated degradation synovial inflammation vivo. Moreover, transient receptor potential vanilloid 4 (TRPV4) was activated during progression OA. Enhanced TRPV4 activation facilitated osteoclast differentiation, inhibition blocked process vitro. repressed vivo inhibiting activation. Our findings demonstrated topical, strategy treatment, regulated inhibition, which may act as target clinical treatment.

Язык: Английский

---

The Current State of Osteoarthritis Treatment Options Using Stem Cells for Regenerative Therapy: A Review

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(10), С. 8925 - 8925

Опубликована: Май 18, 2023

Articular cartilage has very low metabolic activity. While minor injuries may be spontaneously repaired within the joint by chondrocytes, there is little chance of a severely impaired regenerating itself when damaged. Therefore, any significant injury healing without some type therapy. This article review that will examine causes osteoarthritis, both acute and chronic, how it treated using traditional methods as well with latest stem cell technology. The regenerative therapy discussed, including use potential risks mesenchymal cells for tissue regeneration implantation. Applications are then discussed treatment OA in humans after canine animal models. Since most successful research models were dogs, first applications veterinary. However, options have now advanced to point where patients suffering from osteoarthritis this A survey literature was performed order determine current state technology being used osteoarthritis. Then, compared options.

Язык: Английский

---

3D-printed biomimetic scaffolds with precisely controlled and tunable structures guide cell migration and promote regeneration of osteochondral defect

Biofabrication, Год журнала: 2023, Номер 16(1), С. 015003 - 015003

Опубликована: Окт. 5, 2023

Abstract Untreated osteochondral defects will develop into osteoarthritis, affecting patients’ quality of life. Since articular cartilage and subchondral bone exhibit distinct biological characteristics, repairing remains a major challenge. Previous studies have tried to fabricate multilayer scaffolds with traditional methods or 3D printing technology. However, the efficacy is unsatisfactory because poor control over internal structures lack integrity between adjacent layers, severely compromising repair outcomes. Therefore, there need for biomimetic scaffold that can simultaneously boost defect regeneration in both structure function. Herein, an integrated bilayer precisely controlled successfully 3D-printed one step via digital light processing (DLP) The upper layer has ‘lotus- radial-’ distribution pores, bottom ‘lotus-’ pores guide facilitate migration chondrocytes marrow mesenchymal stem cells, respectively, area. Tuning pore sizes could modulate mechanical properties easily. Results show porous allow significantly more cells infiltrate area during cell assay, subcutaneous implantation, situ transplantation, which are essential repair. Transplantation this exhibits promising effect rabbits. Incorporation Kartogenin further induces better formation. Combining small molecules/drugs size-controlled layer-specific DLP technology, expected be potential strategy regeneration.

Язык: Английский

---

Hematopoietic Stem Cells and Their Niche in Bone Marrow

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(13), С. 6837 - 6837

Опубликована: Июнь 21, 2024

Extensive research has explored the functional correlation between stem cells and progenitor cells, particularly in blood. Hematopoietic (HSCs) can self-renew regenerate tissues within bone marrow, while stromal regulate tissue function. Recent studies have validated role of mammalian specific environments, providing initial empirical proof this phenomenon. The interaction blood always been vital to function human body. It was initially proposed that during evolution, formed a complex relationship with surrounding microenvironment, known as niche. Researchers are currently debating significance molecular-level data identify individual cell types due incomplete mapping. Obtaining these help determine activities HSCs marrow. This review summarizes key topics from previous on their environment, discussing current developing concepts related niche

Язык: Английский

---

Atualizações no tratamento da osteoartrite de joelho

Revista Brasileira de Ortopedia, Год журнала: 2024, Номер 59(03), С. e337 - e348

Опубликована: Июнь 1, 2024

Resumo A osteoartrite (OA) do joelho é uma doença inflamatória e degenerativa que ocasiona a destruição da cartilagem articular leva à perda de funções. Sua prevalência vem crescendo consideravelmente devido ao aumento expectativa vida obesidade, o diagnóstico pode ser feito por meio avaliação exames médicos, confirmado em imagens radiográficas complementares. Uma condição multifatorial, OA influenciada diversos aspectos locais, sistêmicos externos; além disso, sua evolução as respostas aos tratamentos dependem muito das características cada indivíduo. Inicialmente, recomenda-se proceder um tratamento medicamentoso terapias alternativas melhorem qualidade paciente; mas, partir momento se verifica tais não estão proporcionando resultados satisfatórios, cirúrgico deve considerado. Entre os cirúrgicos, artroscopias, osteotomias artroplastias parciais totais são destacadas; métodos cirúrgicos incluem uso medicamentos alternativas, como infiltrações, acupuntura prática exercícios físicos. Vale ressaltar ainda utilização biomarcadores importante estratégia para detectar precocemente doença, avaliar atividade, prever prognóstico monitorar melhor resposta terapia; porém, esse tema foco mais estudos seus sejam comprovados.

---

Mesenchymal Stem Cells and Their Exocytotic Vesicles

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(3), С. 2085 - 2085

Опубликована: Янв. 20, 2023

Mesenchymal stem cells (MSCs), as a kind of pluripotent cells, have attracted much attention in orthopedic diseases, geriatric metabolic and sports functions due to their osteogenic potential, chondrogenic differentiation ability, adipocyte differentiation. Anti-inflammation, anti-fibrosis, angiogenesis promotion, neurogenesis, immune regulation, secreted growth factors, proteases, hormones, cytokines, chemokines MSCs been widely studied liver kidney cardiovascular cerebrovascular diseases. In recent years, many studies shown that the extracellular vesicles similar transplantation all above aspects. Here we review research progress exocrine years.

Язык: Английский

---

Mesenchymal stromal/stem cells and their extracellular vesicles in liver diseases: insights on their immunomodulatory roles and clinical applications

Cell & Bioscience, Год журнала: 2023, Номер 13(1)

Опубликована: Сен. 5, 2023

Abstract Liver disease is a leading cause of mortality and morbidity that rising globally. dysfunctions are classified into acute chronic diseases. Various insults, including viral infections, alcohol or drug abuse, metabolic overload, may inflammation fibrosis, to irreversible liver dysfunction. Up now, transplantation could be the last resort for patients with end-stage disease. However, still faces unavoidable difficulties. Mesenchymal stromal/stem cells (MSCs) their broad ranging anti-inflammatory immunomodulatory properties can effectively used treating diseases but without limitation associated transplantation. In this review, we summarize discuss recent advances in characteristics MSCs potential action mechanisms MSCs-based cell therapies We also draw attention strategies potentiate therapeutic through pre-treatments gene modifications. Finally, progress toward clinical application extracellular vesicles

Язык: Английский

---

A novel, microfluidic high-throughput single-cell encapsulation of human bone marrow mesenchymal stromal cells

Journal of Materials Science Materials in Medicine, Год журнала: 2024, Номер 35(1)

Опубликована: Март 25, 2024

Abstract The efficacy of stem-cell therapy depends on the ability transplanted cells to escape early immunological reactions and be retained at site transplantation. use tissue engineering scaffolds or injectable biomaterials as carriers has been proposed, but they still present limitations linked a reliable manufacturing process, surgical practice clinical outcomes. Alginate microbeads are potential candidates for encapsulation mesenchymal stromal with aim providing delivery carrier suitable minimally-invasive scaffold-free transplantation, tissue-adhesive properties protection from immune response. However, formation stable relies cross-linking alginate divalent calcium ions concentrations that toxic cells, making control over beads’ size single-cell unreliable. work demonstrates efficiency an innovative, high throughput, reproducible microfluidic system produce single-cell, calcium-free coatings human cells. Among various conditions tested, visible light confocal microscopy following staining cell nuclei by DAPI showed yielded optimal 2000 cells/min in 2% w/v microcapsules morphology average 28.2 ± 3.7 µm. adhesive microcapsules, viability encapsulated their microcapsule were demonstrated relatively rapid adherence beads onto culture plastic cells’ gradually disrupt shell after 24 h proliferate. To mimic inflammatory response upon exposed proliferating macrophages different seeding densities up 2 days effect assessed time-lapse showing shielding 48 h. This underscores systems precisely encapsulate good standards while favouring retention substrates, proliferation Graphical

Язык: Английский

---

Induced pluripotent stem cells in cartilage tissue engineering: a literature review

Bioscience Reports, Год журнала: 2024, Номер 44(5)

Опубликована: Апрель 2, 2024

Abstract Osteoarthritis (OA) is a long-term, persistent joint disorder characterized by bone and cartilage degradation, resulting in tightness, pain, restricted movement. Current attempts regeneration are cell-based therapies using stem cells. Multipotent cells, such as mesenchymal cells (MSCs), pluripotent embryonic (ESCs), have been used to regenerate cartilage. However, since the discovery of human-induced (hiPSCs) 2007, it was seen potential source for regenerative chondrogenic therapy overcomes ethical issues surrounding use ESCs immunological differentiation limitations MSCs. This literature review focuses on 3D bioprinting technologies hiPSCS, suggesting them viable successful tissue engineering. Methods: A search conducted scientific engines, PubMed, MEDLINE, Google Scholar databases with terms ‘Cartilage engineering’ ‘stem cells’ retrieve published engineering MSCs, ESCs, hiPSCs. Results: hiPSCs may provide an effective autologous treatment focal chondral lesions, though further research needed explore technologies. Conclusions: has provided comprehensive overview these applications medicine.

Язык: Английский

---