Cells,
Год журнала:
2024,
Номер
13(24), С. 2068 - 2068
Опубликована: Дек. 15, 2024
This
comprehensive
overview
of
the
historical
milestones
in
cell
culture
underscores
key
breakthroughs
that
have
shaped
field
over
time.
It
begins
with
Wilhelm
Roux’s
seminal
experiments
1880s,
followed
by
pioneering
efforts
Ross
Granville
Harrison,
who
initiated
groundbreaking
fundamentally
landscape
early
20th
century.
Carrel’s
influential
contributions,
notably
immortalization
chicken
heart
cells,
marked
a
significant
advancement
techniques.
Subsequently,
Johannes
Holtfreter,
Aron
Moscona,
and
Joseph
Leighton
introduced
methodological
innovations
three-dimensional
(3D)
culture,
Alexis
Carrel,
laying
groundwork
for
future
consolidation
expansion
use
3D
different
areas
biomedical
sciences.
The
advent
induced
pluripotent
stem
cells
Takahashi
Yamanaka
2006
was
revolutionary,
enabling
reprogramming
differentiated
into
state.
Since
then,
recent
included
spheroids,
organoids,
organ-on-a-chip
technologies,
aiming
to
mimic
structure
function
tissues
organs
vitro,
pushing
boundaries
biological
modeling
disease
understanding.
In
this
review,
we
history
shedding
light
on
main
discoveries,
pitfalls
hurdles
were
overcome
during
transition
from
2D
Finally,
discussed
directions
research
may
accelerate
development
more
effective
personalized
treatments.
Journal of Hematology & Oncology,
Год журнала:
2023,
Номер
16(1)
Опубликована: Авг. 28, 2023
Triple-negative
breast
cancer
(TNBC),
a
highly
aggressive
subtype
of
cancer,
negatively
expresses
estrogen
receptor,
progesterone
and
the
human
epidermal
growth
factor
receptor
2
(HER2).
Although
chemotherapy
is
main
form
treatment
for
patients
with
TNBC,
effectiveness
TNBC
still
limited.
The
search
more
effective
therapies
urgent.
Multiple
targeted
therapeutic
strategies
have
emerged
according
to
specific
molecules
signaling
pathways
expressed
in
TNBC.
These
include
PI3K/AKT/mTOR
inhibitors,
Notch
poly
ADP-ribose
polymerase
antibody-drug
conjugates.
Moreover,
immune
checkpoint
example,
pembrolizumab,
atezolizumab,
durvalumab,
are
widely
explored
clinic.
We
summarize
recent
advances
therapy
immunotherapy
aim
serving
as
reference
development
individualized
future.
Experimental Hematology and Oncology,
Год журнала:
2024,
Номер
13(1)
Опубликована: Янв. 22, 2024
Abstract
Cancer
immunotherapy
has
emerged
as
a
promising
strategy
in
the
treatment
of
colorectal
cancer,
and
relapse
after
tumor
attracted
increasing
attention.
stem
cells
(CSCs),
small
subset
with
self-renewal
differentiation
capacities,
are
resistant
to
traditional
therapies
such
radiotherapy
chemotherapy.
Recently,
CSCs
have
been
proven
be
driving
immunotherapy.
However,
mutual
interactions
between
cancer
niche
immune
largely
uncharacterized.
In
this
review,
we
focus
on
CSCs,
CSC-immune
cell
CSC-based
Colorectal
characterized
by
robust
expression
surface
markers
CD44,
CD133
Lgr5;
hyperactivation
stemness-related
signaling
pathways,
Wnt/β-catenin,
Hippo/Yap1,
Jak/Stat
Notch
pathways;
disordered
epigenetic
modifications,
including
DNA
methylation,
histone
modification,
chromatin
remodeling,
noncoding
RNA
action.
Moreover,
express
abnormal
levels
immune-related
genes
MHC
checkpoint
molecules
mutually
interact
multiple
tumorigenesis-related
processes,
initiation,
maintenance,
metastasis
drug
resistance.
To
date,
many
targeting
evaluated,
monoclonal
antibodies,
antibody‒drug
conjugates,
bispecific
vaccines
adoptive
therapy,
molecule
inhibitors.
With
development
CSC-/niche-targeting
technology,
well
integration
multidisciplinary
studies,
novel
that
eliminate
reverse
their
immunosuppressive
microenvironment
expected
developed
for
solid
tumors,
cancer.
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
13(9)
Опубликована: Янв. 24, 2024
3D
cell
culture
is
becoming
increasingly
important
for
mimicking
physiological
tissue
structures
in
areas
such
as
drug
discovery
and
personalized
medicine.
To
enable
reproducibility
on
a
large
scale,
automation
technologies
standardized
handling
are
still
challenge.
Here,
novel
method
fully
automated
size
classification
of
aggregates
like
spheroids
organoids
presented.
Using
microfluidic
flow
generated
by
piezoelectric
droplet
generator,
aspirated
from
reservoir
one
side
thin
capillary
deposited
the
other
side,
encapsulated
free-flying
nanoliter
droplets
to
target.
The
platform
has
aggregate
aspiration
plating
efficiencies
98.1%
98.4%,
respectively,
at
processing
throughput
up
21
per
minute.
Cytocompatibility
thoroughly
assessed
with
MCF7,
LNCaP,
A549
colon
organoids,
revealing
no
adverse
effects
shear
stress
reduced
compared
manual
pipetting.
Further,
generic
size-selective
heterogeneous
organoid
samples,
single-aggregate-dispensing
100%
successful
embedding
or
hydrogel
subsequent
proliferation
demonstrated.
This
powerful
tool
vitro
research.
Current Research in Biotechnology,
Год журнала:
2024,
Номер
7, С. 100210 - 100210
Опубликована: Янв. 1, 2024
Cancer
incidence
and
mortality
are
increasing
globally.
immunotherapies,
such
as
immune
checkpoint
inhibitors
adoptive
cell
therapy,
have
been
recognized
a
revolutionary
treatment
approach
to
combat
cancer.
However,
immunotherapeutic
resistance
cancer
recurrence
after
immunotherapy
alarm
us
further
explore
the
underlying
mechanisms
develop
new
immunotherapies.
Experimental
models
hold
great
value
in
research
studies
deciphering
mechanism
of
tumor
initiation
growth,
drug
discovery,
evaluation
efficacy.
The
ideal
model
is
expected
recapitulate
mimic
human
microenvironment,
including
biological,
physiological,
immunologic
functionality.
each
has
its
pros
cons,
selection
depends
on
many
factors,
features,
study
aims,
availability
related
resources.
In
this
review,
we
discussed
commonly
used
currently
immunotherapy,
2D
3D
vitro
culture
spheroid,
organoid,
hydrogel
model,
microfluidic
chip,
vivo
mouse
genetically
engineered
models,
chemically
induced
cell-derived
xenograft
(CDX)
patient-derived
(PDX)
humanized
models.
Both
preclinical
powerful
tools
for
studying
but
all
these
their
limitations.
To
promote
success
clinical
advanced
systems
that
can
better
environment
host
response
preferable
options
study.
Pharmaceutical Development and Technology,
Год журнала:
2025,
Номер
unknown, С. 1 - 19
Опубликована: Янв. 7, 2025
In
this
paper,
the
pH-sensitive
targeting
functional
material
NGR-poly(2-ethyl-2-oxazoline)-cholesteryl
methyl
carbonate
(NGR-PEtOz-CHMC,
NPC)
modified
quercetin
(QUE)
liposomes
(NPC-QUE-L)
was
constructed.
The
structure
of
NPC
confirmed
by
infrared
spectroscopy
(IR)
and
nuclear
magnetic
resonance
hydrogen
spectrum
(1H-NMR).
Pharmacokinetic
results
showed
that
accumulation
QUE
in
plasma
NPC-QUE-L
group
1.28
times
2.43
Solution
QUE-L
groups,
respectively.
release
amount
an
acidic
environment
significantly
higher
than
physiological
pH
value.
order
tumor
cell
inhibition
rate
different
environments
>
PC-QUE-L
QUE-L.
addition,
cellular
uptake
NPC-modified
PC-modified
unmodified
liposomes,
indicating
had
good
pH-sensitivity
targeting.
triple-negative
breast
cancer
(TNBC)
model,
relative
proliferation
is
about
73%,
which
better
solution
group.
Western
blot
show
can
effectively
reduce
expression
α-smooth
actin
transforming
growth
factor-β1
tissues,
improve
degree
fibrosis.
study,
could
endow
with
stability,
pH-sensitivity,
targeting,
provides
a
reference
for
improving
solubility
poorly
soluble
natural
drug
components.
