A Method to Study Migration and Invasion of Mouse Intestinal Organoids DOI Creative Commons
Valérie M. Wouters, Ciro Longobardi, Jan Paul Medema

et al.

Organoids, Journal Year: 2024, Volume and Issue: 3(3), P. 194 - 202

Published: Aug. 21, 2024

Colorectal cancer (CRC) is the third most common worldwide and it second leading cause of death. In CRC, as in cancers, formation metastasis through migration invasion cells to distant organs associated with a dismal prognosis. The study mechanisms cancer, and, particular, changed last decade due introduction organoids. These represent step forward terms complexity from cell lines allowed use mouse models research be limited. Although organoids faithfully model cellular current protocols do not allow for some crucial processes metastasis, such invasion. this study, method using intestinal vitro presented. This protocol provides researchers opportunity investigate migratory behavior organoid impact distinct mutations on invasive capacity cells.

Language: Английский

Recent advances in targeted strategies for triple-negative breast cancer DOI Creative Commons
Shuangli Zhu, Yuze Wu, Bin Song

et al.

Journal of Hematology & Oncology, Journal Year: 2023, Volume and Issue: 16(1)

Published: Aug. 28, 2023

Triple-negative breast cancer (TNBC), a highly aggressive subtype of cancer, negatively expresses estrogen receptor, progesterone and the human epidermal growth factor receptor 2 (HER2). Although chemotherapy is main form treatment for patients with TNBC, effectiveness TNBC still limited. The search more effective therapies urgent. Multiple targeted therapeutic strategies have emerged according to specific molecules signaling pathways expressed in TNBC. These include PI3K/AKT/mTOR inhibitors, Notch poly ADP-ribose polymerase antibody-drug conjugates. Moreover, immune checkpoint example, pembrolizumab, atezolizumab, durvalumab, are widely explored clinic. We summarize recent advances therapy immunotherapy aim serving as reference development individualized future.

Language: Английский

Citations

119
Crosstalk between colorectal CSCs and immune cells in tumorigenesis, and strategies for targeting colorectal CSCs DOI Creative Commons
Qi Zhao, Hong Zong, Pingping Zhu

et al.

Experimental Hematology and Oncology, Journal Year: 2024, Volume and Issue: 13(1)

Published: Jan. 22, 2024

Abstract Cancer immunotherapy has emerged as a promising strategy in the treatment of colorectal cancer, and relapse after tumor attracted increasing attention. stem cells (CSCs), small subset with self-renewal differentiation capacities, are resistant to traditional therapies such radiotherapy chemotherapy. Recently, CSCs have been proven be driving immunotherapy. However, mutual interactions between cancer niche immune largely uncharacterized. In this review, we focus on CSCs, CSC-immune cell CSC-based Colorectal characterized by robust expression surface markers CD44, CD133 Lgr5; hyperactivation stemness-related signaling pathways, Wnt/β-catenin, Hippo/Yap1, Jak/Stat Notch pathways; disordered epigenetic modifications, including DNA methylation, histone modification, chromatin remodeling, noncoding RNA action. Moreover, express abnormal levels immune-related genes MHC checkpoint molecules mutually interact multiple tumorigenesis-related processes, initiation, maintenance, metastasis drug resistance. To date, many targeting evaluated, monoclonal antibodies, antibody‒drug conjugates, bispecific vaccines adoptive therapy, molecule inhibitors. With development CSC-/niche-targeting technology, well integration multidisciplinary studies, novel that eliminate reverse their immunosuppressive microenvironment expected developed for solid tumors, cancer.

Language: Английский

Citations

12
Simultaneous inhibition of FAK and ROS1 synergistically repressed triple-negative breast cancer by upregulating p53 signalling DOI Creative Commons

Ximin Tan,

Deguang Kong,

Zhuoli Tao

et al.

Biomarker Research, Journal Year: 2024, Volume and Issue: 12(1)

Published: Jan. 25, 2024

Abstract Background Triple-negative breast cancer (TNBC) is an aggressive subtype lacking effective targeted therapies, necessitating innovative treatment approaches. While targeting ROS proto-oncogene 1 (ROS1) with crizotinib has shown promise, resistance remains a limitation. Recent evidence links focal adhesion kinase (FAK) to drug resistance, prompting our study assess the combined impact of FAK inhibitor IN10018 and in TNBC elucidate underlying mechanisms. Methods We employed Timer database analyze ROS1 mRNA levels adjacent normal tissues. Furthermore, we investigated correlation between FAK, ROS1, clinical prognosis using GSE database. conducted various vitro assays, including cell viability, colony formation, flow cytometry, EdU western blotting. Additionally, xenograft human organoid models were established therapy’s efficacy. To comprehensively understand synergistic anti-tumor mechanisms, utilized multiple techniques, such as RNA sequencing, immunofluorescence, C11-BODIPY staining, MDA assay, GSH assay. Results The revealed higher tissues compared Analysis GEO databases indicated that patients high expression had poorest prognosis. Western blotting confirmed increased p-FAK crizotinib-resistant cells. In experiments showed combination therapy down-regulated cyclin B1, p-Cdc2, Bcl2 while up-regulating BAX, cleaved-Caspase-3, cleaved-Caspase-9, cleaved PARP. models, tumor volume group was 73% smaller control ( p < 0.0001). resulted 70% reduction viability sequencing analysis cells highlighted enrichment oxidative stress, glutathione metabolism, p53 pathways. displayed fivefold rise reactive oxygen species level, 69% decrease GSH/GSSG ratio, sixfold increase lipid peroxidation comparison group. demonstrated upregulation SCL7A11 GPX4 downregulation addition reversed these effects. Conclusion Our demonstrates shows antitumor effects TNBC. Mechanistically, this inhibits proliferation, enhances apoptosis, induces ferroptosis, which associated levels.

Language: Английский

Citations

9
Recent progress on the organoids: Techniques, advantages and applications DOI
Xiaofeng Liu, Zhiyuan Zhou, Yao Zhang

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2025, Volume and Issue: 185, P. 117942 - 117942

Published: March 4, 2025

Language: Английский

Citations

1
Towards Automation in 3D Cell Culture: Selective and Gentle High‐Throughput Handling of Spheroids and Organoids via Novel Pick‐Flow‐Drop Principle DOI Creative Commons
Viktoria Zieger,

Daniel Frejek,

Stefan Zimmermann

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(9)

Published: Jan. 24, 2024

3D cell culture is becoming increasingly important for mimicking physiological tissue structures in areas such as drug discovery and personalized medicine. To enable reproducibility on a large scale, automation technologies standardized handling are still challenge. Here, novel method fully automated size classification of aggregates like spheroids organoids presented. Using microfluidic flow generated by piezoelectric droplet generator, aspirated from reservoir one side thin capillary deposited the other side, encapsulated free-flying nanoliter droplets to target. The platform has aggregate aspiration plating efficiencies 98.1% 98.4%, respectively, at processing throughput up 21 per minute. Cytocompatibility thoroughly assessed with MCF7, LNCaP, A549 colon organoids, revealing no adverse effects shear stress reduced compared manual pipetting. Further, generic size-selective heterogeneous organoid samples, single-aggregate-dispensing 100% successful embedding or hydrogel subsequent proliferation demonstrated. This powerful tool vitro research.

Language: Английский

Citations

8
Initiation of Cancer: The Journey From Mutations in Somatic Cells to Epigenetic Changes in Tissue-resident VSELs DOI
Deepa Bhartiya, Sherif Raouf, Kshama Pansare

et al.

Stem Cell Reviews and Reports, Journal Year: 2024, Volume and Issue: 20(4), P. 857 - 880

Published: March 8, 2024

Language: Английский

Citations

7
In vitro and in vivo experimental models for cancer immunotherapy study DOI Creative Commons
Chunye Zhang, Yuxiang Sui, Shuai Liu

et al.

Current Research in Biotechnology, Journal Year: 2024, Volume and Issue: 7, P. 100210 - 100210

Published: Jan. 1, 2024

Cancer incidence and mortality are increasing globally. immunotherapies, such as immune checkpoint inhibitors adoptive cell therapy, have been recognized a revolutionary treatment approach to combat cancer. However, immunotherapeutic resistance cancer recurrence after immunotherapy alarm us further explore the underlying mechanisms develop new immunotherapies. Experimental models hold great value in research studies deciphering mechanism of tumor initiation growth, drug discovery, evaluation efficacy. The ideal model is expected recapitulate mimic human microenvironment, including biological, physiological, immunologic functionality. each has its pros cons, selection depends on many factors, features, study aims, availability related resources. In this review, we discussed commonly used currently immunotherapy, 2D 3D vitro culture spheroid, organoid, hydrogel model, microfluidic chip, vivo mouse genetically engineered models, chemically induced cell-derived xenograft (CDX) patient-derived (PDX) humanized models. Both preclinical powerful tools for studying but all these their limitations. To promote success clinical advanced systems that can better environment host response preferable options study.

Language: Английский

Citations

5
NGR-poly(2-ethyl-2-oxazoline)-cholesteryl methyl carbonate enhances the antitumor effect of quercetin liposomes in triple-negative breast cancer DOI
Chengcheng Zhao, Jian Qin,

Dingyu Zhang

et al.

Pharmaceutical Development and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 19

Published: Jan. 7, 2025

In this paper, the pH-sensitive targeting functional material NGR-poly(2-ethyl-2-oxazoline)-cholesteryl methyl carbonate (NGR-PEtOz-CHMC, NPC) modified quercetin (QUE) liposomes (NPC-QUE-L) was constructed. The structure of NPC confirmed by infrared spectroscopy (IR) and nuclear magnetic resonance hydrogen spectrum (1H-NMR). Pharmacokinetic results showed that accumulation QUE in plasma NPC-QUE-L group 1.28 times 2.43 Solution QUE-L groups, respectively. release amount an acidic environment significantly higher than physiological pH value. order tumor cell inhibition rate different environments > PC-QUE-L QUE-L. addition, cellular uptake NPC-modified PC-modified unmodified liposomes, indicating had good pH-sensitivity targeting. triple-negative breast cancer (TNBC) model, relative proliferation is about 73%, which better solution group. Western blot show can effectively reduce expression α-smooth actin transforming growth factor-β1 tissues, improve degree fibrosis. study, could endow with stability, pH-sensitivity, targeting, provides a reference for improving solubility poorly soluble natural drug components.

Language: Английский

Citations

0
Basic Science, New Target Investigation, and Research in Childhood Cancer DOI

Hafeez Abdelhafeez,

Victor M. Santana

Published: Jan. 1, 2025

Language: Английский

Citations

0
Cell culture techniques for cancer research DOI
Preeti Jain, N. V. Joshi, Sadhna Aggarwal

et al.

Methods in cell biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Citations

0