Microbiomes in agroecosystem: Diversity, function and assembly mechanisms

Environmental Microbiology Reports, Год журнала: 2022, Номер 14(6), С. 833 - 849

Опубликована: Окт. 2, 2022

Abstract Soils are a main repository of biodiversity harbouring immense diversity microbial species that plays central role in fundamental ecological processes and acts as the seed bank for emergence plant microbiome cropland ecosystems. Crop‐associated microbiomes play an important shaping performance, which includes but not limited to nutrient uptake, disease resistance, abiotic stress tolerance. Although our understanding structure function soil has been rapidly advancing, most knowledge comes from ecosystems natural environment. In this review, we present overview current communities along soil–plant continuum agroecosystems. To characterize mechanisms community assembly crop microbiomes, explore how host environmental factors such developmental stage, pathogen invasion, land management shape structure, co‐occurrence patterns, crop‐microbiome interactions. Particularly, relative importance deterministic stochastic is illustrated under different conditions, potential sources keystone taxa described. Finally, highlight few questions perspectives future research.

Язык: Английский

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Metagenomic and machine learning-aided identification of biomarkers driving distinctive Cd accumulation features in the root-associated microbiome of two rice cultivars

ISME Communications, Год журнала: 2023, Номер 3(1)

Опубликована: Фев. 22, 2023

Abstract Developing low-cadmium (Cd) rice cultivars has emerged as a promising avenue for food safety in Cd-contaminated farmlands. The root-associated microbiomes of have been shown to enhance growth and alleviate Cd stress. However, the microbial taxon-specific resistance mechanisms underlying different accumulation characteristics between remain largely unknown. This study compared low-Cd cultivar XS14 hybrid YY17 with five soil amendments. results showed that was characterized by more variable community structures stable co-occurrence networks soil-root continuum YY17. stronger stochastic processes assembly (~25%) rhizosphere than (~12%) suggested may higher changes properties. Microbial machine learning models jointly identified keystone indicator microbiota, such Desulfobacteria Nitrospiraceae Meanwhile, genes involved sulfur cycling nitrogen were observed among microbiome these two cultivars, respectively. Microbiomes root diversity functioning, significant enrichment functional related amino acid carbohydrate transport metabolism, cycling. Our findings revealed differences similarities communities associated well bacterial biomarkers predictive Cd-accumulation capacity. Thus, we provide new insights into recruitment strategies under stress highlight utility offering clues enhancing crop resilience stresses future.

Язык: Английский

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Endophytic Beauveria bassiana promotes plant biomass growth and suppresses pathogen damage by directional recruitment

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Авг. 16, 2023

Introduction Entomopathogenic fungi (EPF) can colonize and establish symbiotic relationships with plants as endophytes. Recently, EPF have been reported to suppress plant pathogens induce resistance diseases. However, the potential mechanisms via which endophytes control major diseases in situ remain largely unknown. Methods Pot field experiments were conducted investigate an EPF, Beauveria bassiana , colonizes tomato, under Botrytis cinerea infection stress. B. blastospores inoculated into tomato by root irrigation. Tomato gray mold caused was evaluated artificial inoculation, colonization rhizosphere soil stress colony counting quantitative PCR. Furthermore, expression levels of three disease resistance-related genes ( OXO CHI atpA ) leaves determined explore effect on performance pot experiments. Results could improve . The incidence rate, lesion diameter, index decreased both following colonization. more likely accumulate pathogen infected leaves, while decreasing soil, induced genes, up-regulated leaves. Discussion results indicated that “recruit” from diseased directional effects, then enhanced growth against pathogens, consequently inhibiting multiplication plants. Our findings provide novel insights enhance our understanding roles during challenge.

Язык: Английский

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Plant-microbiome crosstalk and disease development

Current Opinion in Plant Biology, Год журнала: 2023, Номер 72, С. 102351 - 102351

Опубликована: Фев. 26, 2023

Язык: Английский

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Microbiota and functional analyses of nitrogen-fixing bacteria in root-knot nematode parasitism of plants

Microbiome, Год журнала: 2023, Номер 11(1)

Опубликована: Март 10, 2023

Abstract Background Root-knot nematodes (RKN) are among the most important root-damaging plant-parasitic nematodes, causing severe crop losses worldwide. The plant rhizosphere and root endosphere contain rich diverse bacterial communities. However, little is known about how RKN bacteria interact to impact parasitism health. Determining keystone microbial taxa their functional contributions health development for understanding developing efficient biological control strategies in agriculture. Results analyses of microbiota plants with without showed that host species, developmental stage, ecological niche, nematode parasitism, as well interactions, contributed significantly variations root-associated microbiota. Compared healthy tomato at different stages, significant enrichments belonging Rhizobiales, Betaproteobacteriales, Rhodobacterales were observed endophytic nematode-parasitized samples. Functional pathways related pathogenesis nitrogen fixation enriched plants. In addition, we nifH gene NifH protein, key gene/enzyme involved fixation, within roots, consistent a potential contribution nitrogen-fixing parasitism. Data from further assay soil amendment could reduce both prevalence galling Conclusions demonstrated (1) community variation assembly affected by parasitism; (2) taxonomic association was found (3) change communities through addition fertilizers affect occurrence RKN. Our results provide new insights into interactions microbiota, RKN, plants, contributing novel management against

Язык: Английский

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Plant Disease Resistance-Related Pathways Recruit Beneficial Bacteria by Remodeling Root Exudates upon Bacillus cereus AR156 Treatment

Microbiology Spectrum, Год журнала: 2023, Номер 11(2)

Опубликована: Фев. 14, 2023

Our data suggest that B. cereus AR156 can promote the enrichment of beneficial microorganisms in plant rhizosphere by regulating salicylic acid (SA) and jasmonic (JA)/ethylene (ET) signaling pathways plants, thereby playing a role controlling bacterial wilt disease. Meanwhile, Spearman correlation analysis showed relative abundances these bacteria were correlated with secretion root exudates.

Язык: Английский

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Potential effects of micro- and nanoplastics on phyllosphere microorganisms and their evolutionary and ecological responses

The Science of The Total Environment, Год журнала: 2023, Номер 884, С. 163760 - 163760

Опубликована: Апрель 28, 2023

Язык: Английский

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Phyllosphere Microbiome in Plant Health and Disease

Plants, Год журнала: 2023, Номер 12(19), С. 3481 - 3481

Опубликована: Окт. 5, 2023

The phyllosphere refers to the aboveground surface of plants colonized by diverse microorganisms. Microbes inhabiting this environment play an important role in enhancing host's genomic and metabolic capabilities, including defense against pathogens. Compared large volume studies on rhizosphere microbiome for plant health defense, our understanding remains its infancy. In review, we aim explore mechanisms that govern assembly their function host defence, as well highlight knowledge gaps. These efforts will help develop strategies harness toward sustainable crop production.

Язык: Английский

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Rhizosphere Bacteria Help to Compensate for Pesticide-Induced Stress in Plants

Environmental Science & Technology, Год журнала: 2024, Номер 58(28), С. 12542 - 12553

Опубликована: Июль 5, 2024

Although exogenous chemicals frequently exhibit a biphasic response in regulating plant growth, characterized by low-dose stimulation and high-dose inhibition, the underlying mechanisms remain elusive. This study demonstrates, for first time, compensatory function of rhizosphere microbiota assisting plants to withstand pesticide stress. It was observed that pak choi plants, foliar-spraying imidacloprid at both low high doses, could increase total number bacteria enrich numerous beneficial bacteria. These have capabilities promoting growth degrading pesticide, such as Nocardioides, Brevundimonas, Sphingomonas. The bacterial communities were recruited stressed through increasing release primary metabolites root exudates, amino acids, fatty lysophosphatidylcholines. At doses application, microbial effect overcame stress, leading promotion. However, with insufficient counteract resulting inhibition. findings pave way designing improved application strategies contribute better understanding how can be used an eco-friendly approach mitigate chemical-induced stress crops.

Язык: Английский

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From phyllosphere to insect cuticles: silkworms gather antifungal bacteria from mulberry leaves to battle fungal parasite attacks

Microbiome, Год журнала: 2024, Номер 12(1)

Опубликована: Фев. 26, 2024

Abstract Background Bacterial transfers from plants to insect herbivore guts have been well investigated. However, bacterial exchanges between plant phyllospheres and cuticles remain unclear, as does their related biological function. Results Here, we report that the cuticular loads of silkworm larvae quickly increased after molting feeding on white mulberry ( Morus alba ) leaves. The isolation examination bacteria identified one bacterium Mammaliicoccus sciuri could completely inhibit spore germination fungal entomopathogens Metarhizium robertsii Beauveria bassiana . Interestingly, Ma. was evident originally leaves, which produce a secreted chitinolytic lysozyme (termed Msp1) damage cell walls. In consistency, deletion Msp1 substantially impaired antifungal activity. Pretreating with cells followed by topical infections revealed this help defend silkworms against infections. Unsurprisingly, protective efficacy Δ considerably reduced when compared wild-type bacterium. Administration bacterium-treated diets had no negative effect development; instead, supplementation protect artificial diet Aspergillus contamination. Conclusions results study evidence cross-kingdom transfer can insects parasite attacks.

Язык: Английский

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