Extracellular Vesicles in the Repair of Bone and Cartilage Injury: from Macro‐Delivery to Micro‐Modification

Advanced Therapeutics, Год журнала: 2024, Номер 7(6)

Опубликована: Апрель 30, 2024

Abstract Extracellular vesicles (EVs) are intermediaries in intercellular signal transmission and material exchange have attracted significant attention from researchers bone cartilage repair. These nanoscale hold immense potential facilitating repair regeneration by regulating the microenvironment at an injury site. However, their vivo utilization is limited self‐clearance random distribution. Therefore, various delivery platforms been developed to improve EV targeting retention rates target organs while achieving a controlled release of EVs. Additionally, engineering modification EVs has proposed effectively enhance EVs' intrinsic drug‐loading abilities further therapeutic effects on injuries. This review aims introduce biogenesis regulatory mechanisms injuries comprehensively discuss application EV‐delivery different materials methods treating The review's findings can help advance research develop new strategies for improving therapy

Язык: Английский

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Mechanisms of extracellular vesicle uptake and implications for the design of cancer therapeutics

Journal of Extracellular Biology, Год журнала: 2024, Номер 3(11)

Опубликована: Окт. 30, 2024

The translation of pre-clinical anti-cancer therapies to regulatory approval has been promising, but slower than hoped. While innovative and effective treatments continue achieve or seek approval, setbacks are often attributed a lack efficacy, failure clinical endpoints, dose-limiting toxicities. Successful efforts have characterized by the development therapeutics designed specifically deliver optimal dosing tumour cells while minimizing off-target toxicity. Much effort devoted rational design application synthetic nanoparticles serve as targeted therapeutic delivery vehicles. Several challenges successful this modality vehicles include induction protracted immune response that results in their rapid systemic clearance, manufacturing cost, stability, biocompatibility. Extracellular vesicles (EVs) heterogeneous class endogenous biologically produced lipid bilayer mediate intercellular communication carrying bioactive macromolecules capable modifying cellular phenotypes local distant cells. By genetic, chemical, metabolic methods, extracellular can be engineered display targeting moieties on surface transporting specific cargo modulate pathological processes following uptake target cell populations. This review will survey types EVs, composition cargoes, strategies employed increase targeting, uptake, release, potential

Язык: Английский

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Hybrid Nanoparticle Engineered with Transforming Growth Factor -β1-Overexpressed Extracellular Vesicle and Cartilage-Targeted Anti-Inflammatory Liposome for Osteoarthritis

ACS Nano, Год журнала: 2024, Номер unknown

Опубликована: Дек. 8, 2024

Extracellular vesicles (EVs) possess the characteristics of their parent cells, based on which various studies have actively investigated treatments for diseases using mesenchymal stem cell-derived EVs due to regenerative activity. Furthermore, in recent years, there been significant efforts engineer improve native activities and integrate additional functions. Although both endogenous exogenous methods are used engineering EVs, may pose problem administering substances cells undergoing metabolic changes, can cause potential side effects. In addition, limitation losing beneficial factors inside membrane disruption during processes. Surface modification also impair efficiency presence proteins EV surface. Therefore, this study, a stable efficient method was achieved through ethanol-mediated hybridization functionalized lipid nanoparticles (LNPs) with fusogenic component. During hybridization, internal bioactive targeting moiety were maintained LNPs EVs. The Ab-Hybrid, successfully synthesized nicotinamide-encapsulated Col2A1 antibody-modified liposome Transforming growth factor-β1 (TGF-β1)-overexpressed administered osteoarthritis (OA)-induced rats destabilization medial meniscus surgery. Ultimately, Ab-Hybrid demonstrated excellent chondroprotective anti-inflammatory effects long-lasting properties OA lesions. We anticipate that approach manufacturing hybrid particles will serve as valuable versatile platform technology applicable diseases.

Язык: Английский

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Optimizing Conditions of Polyethylene Glycol Precipitation for Exosomes Isolation From MSCs Culture Media for Regenerative Treatment

Biotechnology Journal, Год журнала: 2024, Номер 19(9)

Опубликована: Сен. 1, 2024

ABSTRACT Mesenchymal stem cell (MSC)‐derived exosomes, as a cell‐free alternative to MSCs, offer enhanced safety and significant potential in regenerative medicine. However, isolating these exosomes poses challenge, complicating their broader application. Commonly used methods like ultracentrifugation (UC) tangential flow filtration are often impractical due the requirement for costly instruments ultrafiltration membranes. Additionally, high cost of commercial kits limits use processing large sample volumes. Polyethylene glycol (PEG) precipitation offers more convenient cost‐effective alternative, but there is critical need optimized standardized isolation protocols using PEG across different types fluids ensure consistent quality yield. In this work, we method compared its effectiveness two commonly methods: UC exosome (ExoQuick). The recovery rate (about 61.74%) was comparable that ExoQuick kit 62.19%), which significantly higher than 45.80%). Exosome cargo analysis validated no differences miRNA protein profiles associated with proliferation migration isolated by precipitation, confirmed gap closure CCK8 assays. These findings suggest PEG‐based could be highly efficient high‐quality may facilitate development exosome‐based therapies

Язык: Английский

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Combinatory Nanovesicle with siRNA-Loaded Extracellular Vesicle and IGF-1 for Osteoarthritis Treatments

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(10), С. 5242 - 5242

Опубликована: Май 11, 2024

Extracellular vesicles (EVs) have been found to the characteristics of their parent cells. Based on these EVs, various studies disease treatment using mesenchymal stem cell (MSC)-derived EVs with regenerative activity actively conducted. The therapeutic nature MSC-derived has shown in several studies, but recent years, there many efforts functionalize give them more potent effects. Strategies for functionalizing include endogenous and exogenous methods. In this study, human umbilical cord MSC (UCMSC)-derived were selected optimum OA treatments expectation via bioinformatics analysis based antibody array. And we created a novel nanovesicle system called IGF-si-EV, which properties both cartilage regeneration long-term retention lesion site, attaching positively charged insulin-like growth factor-1 (IGF-1) surface UCMSC-derived Evs carrying siRNA, inhibits MMP13. downregulation inflammation-related cytokine (MMP13, NF-kB, IL-6) upregulation cartilage-regeneration-related factors (Col2, Acan) achieved IGF-si-EV. Moreover, ability IGF-si-EV remain site long time proven through an ex vivo system. Collectively, final constructed can be proposed as effective its successful MMP13 inhibition, chondroprotective effect, adhesion ability. We also believe that EV-based nanoparticle-manufacturing technology applied platform diseases.

Язык: Английский

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Extracellular vesicles: Function, resilience, biomarker, bioengineering, and clinical implications

Tzu Chi Medical Journal, Год журнала: 2024, Номер unknown

Опубликована: Май 24, 2024

Extracellular vesicles (EVs) have emerged as key players in intercellular communication, disease pathology, and therapeutic innovation. Initially overlooked cellular debris, EVs are now recognized vital mediators of cell-to-cell ferrying a cargo proteins, nucleic acids, lipids, providing resilience response to stresses. This review provides comprehensive overview EVs, focusing on their role biomarkers diagnosis, functional significance physiological pathological processes, the potential bioengineering for applications. offer promising avenue noninvasive diagnosis monitoring, reflecting state originating cells. Their diagnostic spans spectrum diseases, including cancer, cardiovascular disorders, neurodegenerative infectious diseases. Moreover, presence bodily fluids such blood, urine, cerebrospinal fluid enhances utility, presenting advantages over traditional methods. Beyond diagnostics, mediate crucial roles facilitating transfer bioactive molecules between communication modulates various processes tissue regeneration, immune modulation, neuronal communication. Dysregulation EV-mediated is implicated diseases highlighting potential. Bioengineering techniques avenues manipulating applications, from isolation purification engineering targeted delivery systems. These approaches hold promise developing novel therapeutics tailored specific revolutionizing personalized medicine. However, challenges standardization, scalability, regulatory approval need addressing successful clinical translation. Overall, represent dynamic frontier biomedical research with vast therapeutics,

Язык: Английский

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Plant-derived exosome-like nanoparticles – from Laboratory to factory, a landscape of application, challenges and prospects

Critical Reviews in Food Science and Nutrition, Год журнала: 2024, Номер unknown, С. 1 - 19

Опубликована: Авг. 11, 2024

Recent decades have witnessed substantial interest in extracellular vesicles (EVs) due to their crucial role intercellular communication across various biological processes. Among these, plant-derived exosome-like Nanoparticles (ELNs) rapidly gained recognition as highly promising candidates. ELNs, characterized by diverse sources, cost-effective production, and straightforward isolation, present a viable option for preventing treating numerous diseases. Furthermore, ELNs hold significant potential carriers natural or engineered drugs, enhancing attractiveness drawing considerable attention science medicine. However, translating into clinical applications poses several challenges. This study explores these challenges offers critical insights research directions. Additionally, it provides forward-looking analysis of the industrial prospects ELNs. With broad remarkable potential, stand at forefront biomedical innovation, poised revolutionize disease management drug delivery paradigms coming years.

Язык: Английский

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Nano-fuels-driven self-sacrificed ZIF-8@Apt integrated chip coupled with a DNAzyme system for the isolation and detection of glioblastoma-derived extracellular vesicles

Chemical Engineering Journal, Год журнала: 2024, Номер 502, С. 157445 - 157445

Опубликована: Ноя. 17, 2024

Язык: Английский

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Nano-Fuels-Driven Self-Sacrificed Zif-8@Apt Integrated Chip Coupled with a Dnazyme System for the Isolation and Detection of Glioblastoma-Derived Extracellular Vesicles

Опубликована: Янв. 1, 2024

Язык: Английский

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Enhanced oral bioavailability of megestrol acetate via polymer-hybridized solid lipid nanoparticles using ultrasonic nebulization method

Journal of Pharmaceutical Investigation, Год журнала: 2024, Номер unknown

Опубликована: Авг. 8, 2024

Язык: Английский

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