Neuron–glia interaction in the Drosophila nervous system

Developmental Neurobiology, Год журнала: 2020, Номер 81(5), С. 438 - 452

Опубликована: Фев. 25, 2020

Abstract Animals are able to move and react in manifold ways external stimuli. Thus, environmental stimuli need be detected, information must processed, and, finally, an output decision transmitted the musculature get animal moving. All these processes depend on nervous system which comprises intricate neuronal network many glial cells. Glial cells have equally important contribution function as their counterpart. Manifold roles attributed glia ranging from controlling cell number axonal pathfinding regulation of synapse formation, function, plasticity. metabolically support neurons contribute blood–brain barrier. aforementioned aspects require extensive cell–cell interactions between Not surprisingly, found all phyla executed by evolutionarily conserved molecules. Here, we review recent advance understanding neuron–glia interaction Drosophila melanogaster suggest that work simple model organisms will shed light mammalian cells, too.

Язык: Английский

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Dilp-2–mediated PI3-kinase activation coordinates reactivation of quiescent neuroblasts with growth of their glial stem cell niche

PLoS Biology, Год журнала: 2020, Номер 18(5), С. e3000721 - e3000721

Опубликована: Май 28, 2020

Dietary nutrients provide macromolecules necessary for organism growth and development. In response to animal feeding, evolutionarily conserved signaling pathways are activated, leading increased rates of cell proliferation tissue growth. It remains unclear how different types within developing tissues coordinate in dietary whether coordinated is proper function. Using the early Drosophila larval brain, we asked nutrient-dependent neural stem cells (neuroblasts), glia, trachea among these major brain required known that PI3-kinase activation, ventral nerve cord neuroblasts reactivate from quiescence glia expand their membranes. Here, assay a cell-type specific manner at short time intervals determine mediated part through activation signaling. Of 7 insulin-like peptides (Dilps), find Dilp-2 coordination between brain. induces cortex initiate membrane make first contact with quiescent neuroblasts. Once reactivated, promote ultimately form selective barrier. Our results highlight importance bidirectional surrounding nutrition demonstrate drives morphogenesis

Язык: Английский

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Drosophila glia take shape to sculpt the nervous system

Current Opinion in Neurobiology, Год журнала: 2023, Номер 79, С. 102689 - 102689

Опубликована: Фев. 22, 2023

Язык: Английский

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Glial Hedgehog signalling and lipid metabolism regulate neural stem cell proliferation in Drosophila

EMBO Reports, Год журнала: 2021, Номер 22(5)

Опубликована: Март 10, 2021

Язык: Английский

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An interplay between cellular growth and atypical fusion defines morphogenesis of a modular glial niche in Drosophila

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Авг. 25, 2022

Abstract Neural stem cells (NSCs) live in an intricate cellular microenvironment supporting their activity, the niche. Whilst shape and function are inseparable, morphogenetic aspects of niche development poorly understood. Here, we use formation a glial to investigate acquisition architectural complexity. Cortex glia (CG) Drosophila regulate neurogenesis build reticular structure around NSCs. We first show that individual CG grow tremendously ensheath several NSC lineages, employing elaborate proliferative mechanisms which convert these into syncytia rich cytoplasmic bridges. further undergo homotypic cell–cell fusion, using defined cell surface receptors actin regulators. Cellular exchange is however dynamic space time. This atypical fusion remodels borders, restructuring syncytia. Ultimately, combined growth builds multi-level architecture niche, creates modular, spatial partition population. Our findings provide insights how forms organises while developing intimate contacts with

Язык: Английский

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Glial ferritin maintains neural stem cells via transporting iron required for self-renewal in Drosophila

eLife, Год журнала: 2024, Номер 13

Опубликована: Март 4, 2024

Stem cell niche is critical for regulating the behavior of stem cells. Drosophila neural cells (Neuroblasts, NBs) are encased by glial closely, but it still remains unclear whether can regulate self-renewal and differentiation NBs. Here, we show that ferritin produced glia, cooperates with Zip13 to transport iron into NBs energy production, which essential proliferation The knockdown encoding genes causes shortage in via downregulating aconitase activity NAD + level, leads low premature mediated Prospero entering nuclei. More importantly, a potential target tumor suppression. In addition, level production affected status NBs, establishing bicellular homeostasis. this study, demonstrate indispensable maintain unveiling novel role NB during brain development.

Язык: Английский

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Expansion and collapse of VEGF diversity in major clades of the animal kingdom

Angiogenesis, Год журнала: 2023, Номер 26(3), С. 437 - 461

Опубликована: Апрель 5, 2023

Together with the platelet-derived growth factors (PDGFs), vascular endothelial (VEGFs) form PDGF/VEGF subgroup among cystine knot factors. The evolutionary relationships within this have not been examined thoroughly to date. Here, we comprehensively analyze throughout all animal phyla and propose a phylogenetic tree. Vertebrate whole-genome duplications play role in expanding diversity, but several limited are necessary account for temporal pattern of emergence. phylogenetically oldest PDGF/VEGF-like factor likely featured C-terminus BR3P signature, hallmark modern-day lymphangiogenic VEGF-C VEGF-D. Some younger VEGF genes, such as VEGFB PGF, appeared completely absent important vertebrate clades birds amphibia, respectively. In contrast, individual gene frequently occurred fish on top known fish-specific duplications. lack precise counterparts human genes poses limitations also offers opportunities research using organisms that diverge considerably from humans. Sources graphical abstract: 326 MYA older [1]; 72-240 [2]; 235-65 [3].

Язык: Английский

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Drosophila ClC‐a is required in glia of the stem cell niche for proper neurogenesis and wiring of neural circuits

Glia, Год журнала: 2019, Номер 67(12), С. 2374 - 2398

Опубликована: Сен. 3, 2019

Abstract Glial cells form part of the neural stem cell niche and express a wide variety ion channels; however, contribution these channels to nervous system development is poorly understood. We explored function Drosophila ClC‐a chloride channel, since its mammalian ortholog CLCN2 expressed in glial cells, defective channel results leukodystrophies, which humans are accompanied by cognitive impairment. found that was cortex glia, closely associated with neurogenic tissues. Characterization loss‐of‐function mutants revealed animals had smaller brains widespread wiring defects. showed required glia for neurogenesis neuroepithelia neuroblasts, identified defects neuroblast lineage generates guidepost essential photoreceptor axon guidance. propose glia‐mediated ionic homeostasis could nonautonomously affect neurogenesis, consequently, correct assembly circuits.

Язык: Английский

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Drosophila melanogaster as a Model System for Human Glioblastomas

Advances in experimental medicine and biology, Год журнала: 2019, Номер unknown, С. 207 - 224

Опубликована: Янв. 1, 2019

Язык: Английский

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Differential adhesion during development establishes individual neural stem cell niches and shapes adult behaviour in Drosophila

PLoS Biology, Год журнала: 2023, Номер 21(11), С. e3002352 - e3002352

Опубликована: Ноя. 9, 2023

Neural stem cells (NSCs) reside in a defined cellular microenvironment, the niche, which supports generation and integration of newborn neurons. The mechanisms building sophisticated niche structure around NSCs their functional relevance for neurogenesis are yet to be understood. In Drosophila larval brain, cortex glia (CG) encase individual NSC lineages membranous chambers, organising cell population neurons into stereotypic structure. We first found that CG wrap lineage-related regardless identity, showing lineage information builds architecture. then discovered mechanism temporally controlled differential adhesion using conserved complexes encasing lineages. An intralineage through homophilic Neuroglian interactions provides strong binding between same lineage, while weaker interaction Neurexin-IV Wrapper exists CG. Loss results clumped together an altered network, loss Neurexin-IV/Wrapper generates larger chamber grouping several together. Axonal projections also these conditions. Further, we link 2 specifically during development locomotor hyperactivity resulting adults. Altogether, our findings identify belt adhesions neurogenic at scale provide proof concept properties shape adult behaviour.

Язык: Английский

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