Tumour-derived gliogenesis sustains dedifferentiation-dependent tumour growth in theDrosophilaCNS DOI Creative Commons
Edel Alvarez‐Ochoa, Qian Dong, Hoa Truong

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

Abstract Fate-restricted cells can acquire stem cell-like properties through dedifferentiation, enabling them to gain the plasticity required for differentiation into multiple lineages. Tumour is prominently observed in brain cancers, where transient cell state changes are linked resistance conventional therapies. In this study, we demonstrate that a sub-population of dedifferentiated tumour neural (NSCs) Drosophila , induced by knockdown prospero ( pros ), generate its own glial niche. Temporal patterning, known influence oncogenic competence and malignancy, plays key role process. Specifically, show de novo gliogenesis occurs more differentiated Syncrip+ (Syp + ) NSC population. Modulating Syp levels alters size niche, subsequently affecting size. Furthermore, tumour-associated niche expands division fails cease proliferation on time due dysregulated ecdysone signalling, contributing expansion. Our findings reveal tumours arising via dedifferentiation establish their supportive microenvironment, which sustains growth.

Language: Английский

Neuron–glia interaction in the Drosophila nervous system DOI Creative Commons

Jonas Bittern,

Nicole Pogodalla,

Henrike Ohm

et al.

Developmental Neurobiology, Journal Year: 2020, Volume and Issue: 81(5), P. 438 - 452

Published: Feb. 25, 2020

Abstract Animals are able to move and react in manifold ways external stimuli. Thus, environmental stimuli need be detected, information must processed, and, finally, an output decision transmitted the musculature get animal moving. All these processes depend on nervous system which comprises intricate neuronal network many glial cells. Glial cells have equally important contribution function as their counterpart. Manifold roles attributed glia ranging from controlling cell number axonal pathfinding regulation of synapse formation, function, plasticity. metabolically support neurons contribute blood–brain barrier. aforementioned aspects require extensive cell–cell interactions between Not surprisingly, found all phyla executed by evolutionarily conserved molecules. Here, we review recent advance understanding neuron–glia interaction Drosophila melanogaster suggest that work simple model organisms will shed light mammalian cells, too.

Language: Английский

Citations

87
Dilp-2–mediated PI3-kinase activation coordinates reactivation of quiescent neuroblasts with growth of their glial stem cell niche DOI Creative Commons
Xin Yuan, Conor W. Sipe, Miyuki Suzawa

et al.

PLoS Biology, Journal Year: 2020, Volume and Issue: 18(5), P. e3000721 - e3000721

Published: May 28, 2020

Dietary nutrients provide macromolecules necessary for organism growth and development. In response to animal feeding, evolutionarily conserved signaling pathways are activated, leading increased rates of cell proliferation tissue growth. It remains unclear how different types within developing tissues coordinate in dietary whether coordinated is proper function. Using the early Drosophila larval brain, we asked nutrient-dependent neural stem cells (neuroblasts), glia, trachea among these major brain required known that PI3-kinase activation, ventral nerve cord neuroblasts reactivate from quiescence glia expand their membranes. Here, assay a cell-type specific manner at short time intervals determine mediated part through activation signaling. Of 7 insulin-like peptides (Dilps), find Dilp-2 coordination between brain. induces cortex initiate membrane make first contact with quiescent neuroblasts. Once reactivated, promote ultimately form selective barrier. Our results highlight importance bidirectional surrounding nutrition demonstrate drives morphogenesis

Language: Английский

Citations

36
Drosophila glia take shape to sculpt the nervous system DOI Creative Commons
Megan M. Corty, Jaeda Coutinho‐Budd

Current Opinion in Neurobiology, Journal Year: 2023, Volume and Issue: 79, P. 102689 - 102689

Published: Feb. 22, 2023

Language: Английский

Citations

12
Glial Hedgehog signalling and lipid metabolism regulate neural stem cell proliferation in Drosophila DOI Creative Commons
Qian Dong, Michael Zavortink,

Francesca Froldi

et al.

EMBO Reports, Journal Year: 2021, Volume and Issue: 22(5)

Published: March 10, 2021

Language: Английский

Citations

26
An interplay between cellular growth and atypical fusion defines morphogenesis of a modular glial niche in Drosophila DOI Creative Commons
Maria A. Rujano, David Briand,

Bojana Ðelić

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Aug. 25, 2022

Abstract Neural stem cells (NSCs) live in an intricate cellular microenvironment supporting their activity, the niche. Whilst shape and function are inseparable, morphogenetic aspects of niche development poorly understood. Here, we use formation a glial to investigate acquisition architectural complexity. Cortex glia (CG) Drosophila regulate neurogenesis build reticular structure around NSCs. We first show that individual CG grow tremendously ensheath several NSC lineages, employing elaborate proliferative mechanisms which convert these into syncytia rich cytoplasmic bridges. further undergo homotypic cell–cell fusion, using defined cell surface receptors actin regulators. Cellular exchange is however dynamic space time. This atypical fusion remodels borders, restructuring syncytia. Ultimately, combined growth builds multi-level architecture niche, creates modular, spatial partition population. Our findings provide insights how forms organises while developing intimate contacts with

Language: Английский

Citations

18
Glial ferritin maintains neural stem cells via transporting iron required for self-renewal in Drosophila DOI Creative Commons
Zhixin Ma, Wenshu Wang, Xiaojing Yang

et al.

eLife, Journal Year: 2024, Volume and Issue: 13

Published: March 4, 2024

Stem cell niche is critical for regulating the behavior of stem cells. Drosophila neural cells (Neuroblasts, NBs) are encased by glial closely, but it still remains unclear whether can regulate self-renewal and differentiation NBs. Here, we show that ferritin produced glia, cooperates with Zip13 to transport iron into NBs energy production, which essential proliferation The knockdown encoding genes causes shortage in via downregulating aconitase activity NAD + level, leads low premature mediated Prospero entering nuclei. More importantly, a potential target tumor suppression. In addition, level production affected status NBs, establishing bicellular homeostasis. this study, demonstrate indispensable maintain unveiling novel role NB during brain development.

Language: Английский

Citations

3
Expansion and collapse of VEGF diversity in major clades of the animal kingdom DOI Creative Commons
Khushbu Rauniyar, Honey Bokharaie, Michael Jeltsch

et al.

Angiogenesis, Journal Year: 2023, Volume and Issue: 26(3), P. 437 - 461

Published: April 5, 2023

Together with the platelet-derived growth factors (PDGFs), vascular endothelial (VEGFs) form PDGF/VEGF subgroup among cystine knot factors. The evolutionary relationships within this have not been examined thoroughly to date. Here, we comprehensively analyze throughout all animal phyla and propose a phylogenetic tree. Vertebrate whole-genome duplications play role in expanding diversity, but several limited are necessary account for temporal pattern of emergence. phylogenetically oldest PDGF/VEGF-like factor likely featured C-terminus BR3P signature, hallmark modern-day lymphangiogenic VEGF-C VEGF-D. Some younger VEGF genes, such as VEGFB PGF, appeared completely absent important vertebrate clades birds amphibia, respectively. In contrast, individual gene frequently occurred fish on top known fish-specific duplications. lack precise counterparts human genes poses limitations also offers opportunities research using organisms that diverge considerably from humans. Sources graphical abstract: 326 MYA older [1]; 72-240 [2]; 235-65 [3].

Language: Английский

Citations

8
Drosophila ClC‐a is required in glia of the stem cell niche for proper neurogenesis and wiring of neural circuits DOI Creative Commons
Haritz Plazaola‐Sasieta, Qi Zhu, Héctor Gaitán‐Peñas

et al.

Glia, Journal Year: 2019, Volume and Issue: 67(12), P. 2374 - 2398

Published: Sept. 3, 2019

Abstract Glial cells form part of the neural stem cell niche and express a wide variety ion channels; however, contribution these channels to nervous system development is poorly understood. We explored function Drosophila ClC‐a chloride channel, since its mammalian ortholog CLCN2 expressed in glial cells, defective channel results leukodystrophies, which humans are accompanied by cognitive impairment. found that was cortex glia, closely associated with neurogenic tissues. Characterization loss‐of‐function mutants revealed animals had smaller brains widespread wiring defects. showed required glia for neurogenesis neuroepithelia neuroblasts, identified defects neuroblast lineage generates guidepost essential photoreceptor axon guidance. propose glia‐mediated ionic homeostasis could nonautonomously affect neurogenesis, consequently, correct assembly circuits.

Language: Английский

Citations

22
Drosophila melanogaster as a Model System for Human Glioblastomas DOI
Alexander S. Chen, Renee Read

Advances in experimental medicine and biology, Journal Year: 2019, Volume and Issue: unknown, P. 207 - 224

Published: Jan. 1, 2019

Language: Английский

Citations

13
Differential adhesion during development establishes individual neural stem cell niches and shapes adult behaviour in Drosophila DOI Creative Commons

Agata Banach-Latapy,

Vincent Rincheval,

David Briand

et al.

PLoS Biology, Journal Year: 2023, Volume and Issue: 21(11), P. e3002352 - e3002352

Published: Nov. 9, 2023

Neural stem cells (NSCs) reside in a defined cellular microenvironment, the niche, which supports generation and integration of newborn neurons. The mechanisms building sophisticated niche structure around NSCs their functional relevance for neurogenesis are yet to be understood. In Drosophila larval brain, cortex glia (CG) encase individual NSC lineages membranous chambers, organising cell population neurons into stereotypic structure. We first found that CG wrap lineage-related regardless identity, showing lineage information builds architecture. then discovered mechanism temporally controlled differential adhesion using conserved complexes encasing lineages. An intralineage through homophilic Neuroglian interactions provides strong binding between same lineage, while weaker interaction Neurexin-IV Wrapper exists CG. Loss results clumped together an altered network, loss Neurexin-IV/Wrapper generates larger chamber grouping several together. Axonal projections also these conditions. Further, we link 2 specifically during development locomotor hyperactivity resulting adults. Altogether, our findings identify belt adhesions neurogenic at scale provide proof concept properties shape adult behaviour.

Language: Английский

Citations

4