Insect Molecular Biology,
Год журнала:
2020,
Номер
29(6), С. 545 - 554
Опубликована: Июль 27, 2020
Abstract
Krüppel
homolog1
(Kr‐h1)
is
a
juvenile
hormone
(JH)
response
transcriptional
factor
that
transduces
JH
signalling
to
repress
insect
metamorphosis
in
both
hemimetabolous
and
holometabolous
insects.
While
few
studies
about
microRNAs
(miRNAs)
downregulating
Kr‐h1
expression
mediate
have
been
demonstrated
insects,
the
miRNAs
target
of
insects
not
reported.
Here,
we
identified
two
miR‐927
binding
sites
within
3′UTR
region
Drosophila
melanogaster
,
was
found
downregulate
.
The
profiles
displayed
relatively
opposite
pattern
during
most
larval
development
stages.
Overexpression
fat
body
significantly
decreased
resulted
reduced
oviposition,
increased
mortality,
delayed
pupation,
pupal
size.
Notably,
co‐overexpression
rescued
developmental
growth
defects
associated
with
overexpression,
indicating
biologically
relevant
miR‐927.
Moreover,
be
repressed
by
its
receptor
Met/gce,
forming
positive
regulatory
loop
signalling.
Overall,
our
support
conserved
role
for
JH/miRNA/
axis
control
development.
Abstract
Background
The
zinc-finger
transcription
factor
Krüppel-homolog
1
(Kr-h1)
exerts
a
dual
regulatory
role
during
insect
development
by
preventing
precocious
larval/nymphal
metamorphosis
and
in
stimulating
aspects
of
adult
reproduction
such
as
vitellogenesis.
However,
how
Kr-h1
functions
both
transcriptional
repressor
juvenile
an
activator
remains
elusive.
Here,
we
use
the
Locusta
migratoria
to
dissect
molecular
mechanism
which
at
these
distinct
developmental
stages.
Results
We
report
that
kinase
PKCα
triggers
phosphorylation
amino
acid
residue
Ser
154
,
step
essential
for
its
functions.
During
stage,
phosphorylated
recruits
corepressor,
C-terminal
binding
protein
(CtBP).
complex
CtBP
represses
Ecdysone
induced
93F
(
E93
)
consequently
prevents
juvenile-to-adult
transition.
In
insects,
coactivator,
CREB-binding
(CBP),
promotes
vitellogenesis
inducing
expression
Ribosomal
L36
.
Furthermore,
with
concomitant
inhibition
is
evolutionarily
conserved
across
orders.
Conclusion
Our
results
suggest
indispensable
recruitment
cofactors,
anti-metamorphic
vitellogenic
actions
insects.
data
shed
new
light
on
understanding
regulation
function
JH-regulated
reproduction.
PLoS Genetics,
Год журнала:
2022,
Номер
18(9), С. e1010411 - e1010411
Опубликована: Сен. 16, 2022
Fecundity
is
arguably
one
of
the
most
important
life
history
traits,
as
it
closely
tied
to
fitness.
Most
arthropods
are
recognized
for
their
extreme
reproductive
capacity.
For
example,
a
single
female
oriental
fruit
fly
Bactrocera
dorsalis
,
highly
invasive
species
that
destructive
agricultural
pests
worldwide,
can
lay
more
than
3000
eggs
during
its
span.
The
ovary
crucial
insect
reproduction
and
development
requires
further
investigation
at
molecular
level.
We
report
here
miR-309a
regulator
ovarian
in
B
.
Our
bioinformatics
studies
have
revealed
binds
transcription
factor
pannier
(
GATA-binding
A
/
pnr
),
this
activates
yolk
vitellogenin
2
Vg
)
receptor
VgR
advancing
development.
show
under
control
juvenile
hormone
(JH)
independent
from
20-hydroxyecdysone.
Thus,
we
identified
JH-controlled
miR-309a/
axis
regulates
Vg2
This
study
has
enhanced
our
understanding
mechanisms
governing
reproduction.
It
provides
background
identifying
targets
controlling
Dipteran
pests.
Insect Science,
Год журнала:
2022,
Номер
30(2), С. 279 - 292
Опубликована: Июнь 22, 2022
The
forkhead
box
O
(FoxO),
as
a
conserved
transcription
factor,
plays
an
indispensable
role
in
regulating
insect
diapause.
However,
how
FoxO
is
regulated
to
control
diapause
insects
remains
unknown.
In
this
study,
we
discovered
functional
binding
sites
for
miR-2765-3p
the
3'
untranslated
region
of
Galeruca
daurica.
luciferase
reporter
assay
showed
that
targeted
and
suppressed
its
expression.
expression
profiles
displayed
opposite
patterns
during
female
developmental
process.
Overexpression
by
injection
agomir
into
adult
females
reduced
expression,
leading
suppression
lipid
accumulation,
promotion
ovarian
development,
inhibition
reproductive
This
similar
phenotype
results
from
depletion
injecting
dsFoxO
females.
addition,
repression
antagomir
increased
transcript
level,
stimulation
depression
induction
A
hormone
juvenile
(JH)
agonist
(methoprene)
upregulated
downregulated
FoxO.
Notably,
methoprene
rescued
development
defects
associated
with
inhibition.
These
findings
indicate
JH/miR-2765-3p/FoxO
axis
vital
regulation
G.
Frontiers in Genetics,
Год журнала:
2022,
Номер
12
Опубликована: Янв. 21, 2022
Spodoptera
frugiperda
is
the
world’s
major
agricultural
pest
and
has
distinctive
features
of
high
fecundity,
strong
migratory
capacity,
resistance
to
most
insecticides.
At
present,
control
S.
in
China
relies
mainly
on
spraying
chemical
MicroRNAs
(miRNAs)
are
a
class
small,
single-stranded,
non-coding
RNAs
play
crucial
regulatory
roles
various
physiological
processes,
including
insecticide
insects.
However,
little
known
about
miRNAs
In
present
research,
that
were
differentially
expressed
after
cyantraniliprole,
spinetoram,
emamectin
benzoate
treatment
analyzed
by
RNA-Seq.
A
total
504
systematically
identified
from
,
24,
22,
31
treatments
benzoate.
GO
KEGG
enrichment
analyses
used
predict
function
target
genes
miRNAs.
Importantly,
ten
significantly
among
three
miR-278-5p,
miR-13b-3p,
miR-10485-5p,
miR-10483-5p
downregulated
insecticides
RT-qPCR.
Furthermore,
overexpression
increased
mortality
cyantraniliprole
The
was
with
spinetoram
miR-10483-5p.
These
results
suggest
miRNAs,
which
response
insecticides,
may
key
role
tolerance
.
Molecular Biology and Evolution,
Год журнала:
2022,
Номер
39(6)
Опубликована: Июнь 1, 2022
Abstract
Organisms
residing
in
regions
with
alternating
seasons
often
develop
different
phenotypes,
or
forms,
each
season.
These
forms
are
adaptations
to
season
and
result
from
an
altered
developmental
response
specific
environmental
cues
such
as
temperature.
Although
multiple
studies
have
examined
form-specific
gene
expression
profiles
a
diversity
of
species,
little
is
known
about
how
environments
transitions,
cued
by
hormone
pulses,
alter
post-transcriptional
patterns.
In
this
study,
we
examine
expression,
alternative
splicing,
miRNA-mediated
silencing
Bicyclus
anynana
butterfly
hindwing
tissue,
varies
across
two
rearing
temperatures
at
four
timepoints.
timepoints
flank
temperature-sensitive
periods
that
coincide
pulses
the
insect
20E.
Our
results
suggest
coincident
20E
elicit
greater
impact
on
all
these
transcriptomic
patterns
than
per
se.
More
similar
observed
pre-20E
those
post-20E
pulses.
We
also
found
functionally
distinct
sets
differentially
expressed
genes
(DEGs)
spliced
(DSGs)
seasonal
forms.
Furthermore,
around
10%
DEGs
predicted
be
direct
targets
of,
regulated
by,
miRNAs
(DEmiRs)
between
Many
DEGs,
DEmiRs,
DSGs
potentially
regulate
eyespot
size
plasticity,
validated
differential
splicing
pattern
one
gene,
daughterless.
present
comprehensive
interactive
atlas
tissue
both
B.
throughout
development,
model
organism
plasticity.
