Detection, regulation, and functions of RNA N6-methyladenosine modification in plants

Plant Communications, Год журнала: 2023, Номер 4(3), С. 100546 - 100546

Опубликована: Янв. 10, 2023

N6-Methyladenosine (m6A) is the most abundant internal chemical modification in eukaryotic mRNA and plays important roles gene expression regulation, including transcriptional post-transcriptional regulation. m6A a reversible that installed, removed, recognized by methyltransferases (writers), demethylases (erasers), m6A-binding proteins (readers), respectively. Recently, breadth of research on plants has expanded, vital plant development, biotic abiotic stress responses, crop trait improvement have been investigated. In this review, we discuss recent developments highlight detection methods, distribution, regulatory proteins, molecular biological functions plants. We also offer some perspectives future investigations, providing direction for subsequent

Язык: Английский

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The m6A reader ECT8 is an abiotic stress sensor that accelerates mRNA decay in Arabidopsis

The Plant Cell, Год журнала: 2024, Номер 36(8), С. 2908 - 2926

Опубликована: Июнь 5, 2024

Abstract N 6-methyladenosine (m6A) is the most abundant mRNA modification and plays diverse roles in eukaryotes, including plants. It regulates various processes, plant growth, development, responses to external or internal stress responses. However, mechanisms underlying how m6A related environmental stresses both mammals plants remain elusive. Here, we identified EVOLUTIONARILY CONSERVED C-TERMINAL REGION 8 (ECT8) as an reader protein showed that its m6A-binding capability required for salt Arabidopsis (Arabidopsis thaliana). ECT8 accelerates degradation of target transcripts through direct interaction with decapping DECAPPING 5 within processing bodies. We observed a significant increase expression level under stresses. Using representative stressor, found transcript levels rise response stress. The increased abundance results enhanced binding m6A-modified mRNAs, thereby accelerating their degradation, especially those negative regulators Our demonstrated acts abiotic sensor, facilitating decay, which vital maintaining transcriptome homeostasis enhancing tolerance findings not only advance understanding epitranscriptomic gene regulation but also offer potential applications breeding more resilient crops face rapidly changing conditions.

Язык: Английский

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m6A modification of U6 snRNA modulates usage of two major classes of pre-mRNA 5’ splice site

eLife, Год журнала: 2022, Номер 11

Опубликована: Ноя. 21, 2022

Alternative splicing of messenger RNAs is associated with the evolution developmentally complex eukaryotes. Splicing mediated by spliceosome, and docking pre-mRNA 5’ splice site into spliceosome active depends upon pairing conserved ACAGA sequence U6 snRNA. In some species, including humans, central adenosine AC A GA box modified N 6 methylation, but role this m modification poorly understood. Here, we show that snRNA determines accuracy efficiency splicing. We reveal methyltransferase, FIONA1, required for Arabidopsis modification. fio1 mutants disrupted patterns can be explained composition sites cooperative roles U5 in selection. influences 3’ usage. generalise these findings to two major classes diverse eukaryotes, which display anti-correlated interaction potential loop 1 box. conclude contributes selection degenerate crucial alternative

Язык: Английский

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FLK is an mRNA m6A reader that regulates floral transition by modulating the stability and splicing of FLC in Arabidopsis

Molecular Plant, Год журнала: 2023, Номер 16(5), С. 919 - 929

Опубликована: Апрель 12, 2023

Язык: Английский

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Light-induced LLPS of the CRY2/SPA1/FIO1 complex regulating mRNA methylation and chlorophyll homeostasis in Arabidopsis

Nature Plants, Год журнала: 2023, Номер 9(12), С. 2042 - 2058

Опубликована: Дек. 8, 2023

Abstract Light regulates chlorophyll homeostasis and photosynthesis via various molecular mechanisms in plants. The light regulation of transcription protein stability nuclear-encoded chloroplast proteins have been extensively studied, but how mRNA metabolism affects abundance remains poorly understood. Here we show that the blue receptor cryptochrome 2 (CRY2) METTL16-type m 6 A writer FIONA1 (FIO1) regulate response to light. In contrast CRY2-mediated photo-condensation adenosine methylase (MTA), photoexcited CRY2 co-condenses FIO1 only presence CRY2-signalling SUPPRESSOR PHYTOCHROME (SPA1). SPA1 synergistically or additively activate RNA methyltransferase activity vitro, whereas FIO1, not MTA, are required for light-induced methylation translation mRNAs encoding multiple regulators vivo. Our study demonstrates liquid–liquid phase separation photoreceptor/writer complexes is commonly involved photoresponsive changes methylation, different CRY/FIO1 CRY/MTA explain, at least partially, writer-specific functions plant photomorphogenesis.

Язык: Английский

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FIONA1‐mediated mRNA m⁶A methylation regulates the response of Arabidopsis to salt stress

Plant Cell & Environment, Год журнала: 2024, Номер 47(3), С. 900 - 912

Опубликована: Янв. 9, 2024

Abstract N 6 ‐methyladenosine (m A) is an mRNA modification widely found in eukaryotes and plays a crucial role plant development stress responses. FIONA1 (FIO1) recently identified m A methyltransferase that regulates Arabidopsis ( thaliana ) floral transition; however, its response remains unknown. In this study, we demonstrate FIO1‐mediated methylation vital salt Arabidopsis. The loss‐of‐function fio1 mutant was sensitive to stress. Importantly, the complementation lines expressing wild‐type FIO1 exhibited phenotype, whereas , which two critical amino acid residues essential for activity were mutated, did not recover phenotype under stress, indicating sensitivity associated with activity. Furthermore, regulated ROS production affected transcript level of several stress‐responsive genes via modulating their stability A‐dependent manner by specifically targeting differentially compared other writer. Collectively, our findings highlight molecular mechanism adaptation.

Язык: Английский

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Quantitative profiling of m6A at single base resolution across the life cycle of rice and Arabidopsis

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Июнь 7, 2024

Abstract N 6 -methyladenosine (m A) plays critical roles in regulating mRNA metabolism. However, comprehensive m A methylomes different plant tissues with single-base precision have yet to be reported. Here, we present transcriptome-wide maps at resolution of rice and Arabidopsis using A-SAC-seq. Our analysis uncovers a total 205,691 sites distributed across 22,574 genes rice, 188,282 19,984 . The evolutionarily conserved ortholog gene pairs are involved controlling tissue development, photosynthesis stress response. We observe an overall stabilization effect by 3’ UTR certain tissues. Like mammals, positive correlation between the level length internal exons is also observed mRNA, except for last exon. data suggest active deposition process occurring near stop codon mRNA. In addition, MTA-installed correlate both translation promotion suppression, depicting more complicated regulatory picture. results therefore provide in-depth resources relating functions plants uncover suppression-activation model biogenesis species.

Язык: Английский

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COOLAIR and PRC2 function in parallel to silence FLC during vernalization

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(4)

Опубликована: Янв. 18, 2024

Noncoding transcription induces chromatin changes that can mediate environmental responsiveness, but the causes and consequences of these mechanisms are still unclear. Here, we investigate how antisense (termed COOLAIR ) interfaces with Polycomb Repressive Complex 2 (PRC2) silencing during winter-induced epigenetic regulation Arabidopsis FLOWERING LOCUS C ( FLC ). We use genetic analyses on lines ineffective or hyperactive for pathway in combination computational modeling to define underlying repression. Our results show is silenced through pathways function different dynamics: a transcription-mediated capable fast response parallel slow PRC2 switching mechanism maintains each allele an epigenetically state. Components both regulated by common transcriptional regulator (NTL8), which accumulates reduced dilution due growth at low temperature. The activities regulatory steps, their control temperature-dependent dynamics, create flexible system registering widely fluctuating natural temperature conditions change year year, yet ensure robust FLC.

Язык: Английский

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ArabidopsisN6-methyladenosine methyltransferase FIONA1 regulates floral transition by affecting the splicing ofFLCand the stability of floral activatorsSPL3andSEP3

Journal of Experimental Botany, Год журнала: 2022, Номер 74(3), С. 864 - 877

Опубликована: Ноя. 23, 2022

Abstract N 6-methyladenosine (m6A) RNA methylation has been shown to play a crucial role in plant development and floral transition. Two recent studies have identified FIONA1 as an m6A methyltransferase that regulates the transition Arabidopsis through influencing stability of CONSTANS (CO), SUPPRESSOR OF OVEREXPRESSION 1 (SOC1), FLOWERING LOCUS C (FLC). In this study, we confirmed is installs marks small group mRNAs. Furthermore, show that, addition its CO, SOC1, FLC, FIONA1-mediated influences splicing key repressor, SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE 3 (SPL3) SEPALLATA3 (SEP3), activators, which together vital Arabidopsis. Our study confirms function suggests close molecular link between FLC destabilization SPL3 SEP3 flowering time control.

Язык: Английский

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Principles, functions, and biological implications of m⁶A in plants

RNA, Год журнала: 2024, Номер 30(5), С. 491 - 499

Опубликована: Март 26, 2024

Over the past decade, N 6 -methyladenosine (m A) has emerged as a prevalent and dynamically regulated modification across transcriptome; it been reversibly installed, removed, interpreted by specific binding proteins, played crucial roles in molecular biological processes. Within this scope, we consolidate recent advancements of m A research plants regarding gene expression regulation, diverse physiologic pathogenic processes, well crop trial implications, to guide discussions on challenges associated with leveraging epitranscriptome editing for improvement.

Язык: Английский

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