Yeast,
Год журнала:
2024,
Номер
41(4), С. 148 - 157
Опубликована: Янв. 18, 2024
Abstract
N
6
‐methyladenosine
(m6A)
is
a
highly
abundant
and
evolutionarily
conserved
messenger
RNA
(mRNA)
modification.
This
modification
installed
on
RRACH
motifs
mRNAs
by
hetero‐multimeric
holoenzyme
known
as
m6A
methyltransferase
complex
(MTC).
The
mark
then
recognised
group
of
proteins
the
YTH
domain
family
which
guide
mRNA
for
subsequent
downstream
processes
that
determine
its
fate.
In
yeast,
thousands
during
early
meiosis
MTC
m6A‐modified
are
read
domain‐containing
protein
Mrb1/Pho92.
this
review,
we
aim
to
delve
into
recent
advances
in
our
understanding
regulation
roles
yeast
meiosis.
We
will
discuss
potential
functions
translation
decay,
unravelling
their
significance
regulating
gene
expression.
propose
serves
an
exceptional
model
organism
study
fundamental
molecular
mechanisms
related
function
mRNAs.
insights
gained
from
research
not
only
expand
knowledge
modifications
but
also
offer
valuable
broader
landscape
eukaryotic
posttranscriptional
Science China Life Sciences,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 8, 2025
Abstract
Epigenetic
mechanisms
are
integral
to
plant
growth,
development,
and
adaptation
environmental
stimuli.
Over
the
past
two
decades,
our
comprehension
of
these
complex
regulatory
processes
has
expanded
remarkably,
producing
a
substantial
body
knowledge
on
both
locus-specific
genome-wide
patterns.
Studies
initially
grounded
in
model
Arabidopsis
have
been
broadened
encompass
diverse
array
crop
species,
revealing
multifaceted
roles
epigenetics
physiological
agronomic
traits.
With
recent
technological
advancements,
epigenetic
regulations
at
single-cell
level
large-scale
population
emerging
as
new
focuses.
This
review
offers
an
in-depth
synthesis
regulations,
detailing
catalytic
machinery
functions.
It
delves
into
intricate
interplay
among
various
elements
their
collective
influence
modulation
Furthermore,
it
examines
breakthroughs
technologies
for
modifications
integration
strategies
improvement.
The
underscores
transformative
potential
bolstering
performance,
advocating
development
efficient
tools
fully
exploit
agricultural
benefits
insights.
Plant Cell & Environment,
Год журнала:
2024,
Номер
47(3), С. 900 - 912
Опубликована: Янв. 9, 2024
Abstract
N
6
‐methyladenosine
(m
A)
is
an
mRNA
modification
widely
found
in
eukaryotes
and
plays
a
crucial
role
plant
development
stress
responses.
FIONA1
(FIO1)
recently
identified
m
A
methyltransferase
that
regulates
Arabidopsis
(
thaliana
)
floral
transition;
however,
its
response
remains
unknown.
In
this
study,
we
demonstrate
FIO1‐mediated
methylation
vital
salt
Arabidopsis.
The
loss‐of‐function
fio1
mutant
was
sensitive
to
stress.
Importantly,
the
complementation
lines
expressing
wild‐type
FIO1
exhibited
phenotype,
whereas
,
which
two
critical
amino
acid
residues
essential
for
activity
were
mutated,
did
not
recover
phenotype
under
stress,
indicating
sensitivity
associated
with
activity.
Furthermore,
regulated
ROS
production
affected
transcript
level
of
several
stress‐responsive
genes
via
modulating
their
stability
A‐dependent
manner
by
specifically
targeting
differentially
compared
other
writer.
Collectively,
our
findings
highlight
molecular
mechanism
adaptation.
Plant Communications,
Год журнала:
2024,
Номер
5(10), С. 101037 - 101037
Опубликована: Июль 6, 2024
N6-methyladenosine
(m6A)
is
the
most
abundant
modification
observed
in
eukaryotic
mRNAs.
Advances
transcriptome-wide
m6A
mapping
and
sequencing
technologies
have
enabled
identification
of
several
conserved
motifs
plants,
including
RRACH
(R
=
A/G
H
A/C/U)
UGUAW
(W
U
or
A)
motifs.
However,
mechanisms
underlying
deposition
marks
at
specific
positions
individual
transcripts
remain
to
be
clarified.
Evidence
from
plant
animal
studies
suggests
that
writer
eraser
components
are
recruited
genomic
loci
through
interactions
with
particular
transcription
factors,
5-methylcytosine
DNA
methylation
marks,
histone
marks.
In
addition,
recent
cells
shown
microRNAs
play
a
role
depositing
sites
base-pairing
mechanism.
also
affects
biogenesis
function
chromatin-associated
regulatory
RNAs
long
noncoding
RNAs.
Although
we
less
an
understanding
link
between
epigenetic
factors
plants
than
animals,
progress
identifying
proteins
interact
has
provided
insights
into
crosstalk
which
plays
crucial
transcript-specific
regulation
plants.
Proceedings of the National Academy of Sciences,
Год журнала:
2024,
Номер
121(4)
Опубликована: Янв. 18, 2024
Noncoding
transcription
induces
chromatin
changes
that
can
mediate
environmental
responsiveness,
but
the
causes
and
consequences
of
these
mechanisms
are
still
unclear.
Here,
we
investigate
how
antisense
(termed
COOLAIR
)
interfaces
with
Polycomb
Repressive
Complex
2
(PRC2)
silencing
during
winter-induced
epigenetic
regulation
Arabidopsis
FLOWERING
LOCUS
C
(
FLC
).
We
use
genetic
analyses
on
lines
ineffective
or
hyperactive
for
pathway
in
combination
computational
modeling
to
define
underlying
repression.
Our
results
show
is
silenced
through
pathways
function
different
dynamics:
a
transcription-mediated
capable
fast
response
parallel
slow
PRC2
switching
mechanism
maintains
each
allele
an
epigenetically
state.
Components
both
regulated
by
common
transcriptional
regulator
(NTL8),
which
accumulates
reduced
dilution
due
growth
at
low
temperature.
The
activities
regulatory
steps,
their
control
temperature-dependent
dynamics,
create
flexible
system
registering
widely
fluctuating
natural
temperature
conditions
change
year
year,
yet
ensure
robust
FLC.
Over
the
past
decade,
N
6
-methyladenosine
(m
A)
has
emerged
as
a
prevalent
and
dynamically
regulated
modification
across
transcriptome;
it
been
reversibly
installed,
removed,
interpreted
by
specific
binding
proteins,
played
crucial
roles
in
molecular
biological
processes.
Within
this
scope,
we
consolidate
recent
advancements
of
m
A
research
plants
regarding
gene
expression
regulation,
diverse
physiologic
pathogenic
processes,
well
crop
trial
implications,
to
guide
discussions
on
challenges
associated
with
leveraging
epitranscriptome
editing
for
improvement.
Journal of Integrative Plant Biology,
Год журнала:
2024,
Номер
66(12), С. 2586 - 2599
Опубликована: Окт. 4, 2024
ABSTRACT
Modifications
to
RNA
have
recently
been
recognized
as
a
pivotal
regulator
of
gene
expression
in
living
organisms.
More
than
170
chemical
modifications
identified
RNAs,
with
N
6
‐methyladenosine
(m
A)
being
the
most
abundant
modification
eukaryotic
mRNAs.
The
addition
and
removal
m
A
marks
are
catalyzed
by
methyltransferases
(referred
“writers”)
demethylases
“erasers”),
respectively.
In
addition,
mRNAs
interpreted
A‐binding
proteins
“readers”),
which
regulate
fate
mRNAs,
including
stability,
splicing,
transport,
translation.
Therefore,
exploring
mechanism
underlying
reader‐mediated
modulation
metabolism
is
essential
for
much
deeper
understanding
epigenetic
role
plants.
Recent
discoveries
improved
our
functions
readers
plant
growth
development,
stress
response,
disease
resistance.
This
review
highlights
latest
developments
reader
research,
emphasizing
diverse
RNA‐binding
domains
crucial
function
biological
cellular
roles
response
developmental
environmental
signals.
Moreover,
we
propose
discuss
potential
future
research
directions
challenges
identifying
novel
elucidating
mechanistic
Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Июль 16, 2024
The
epitranscriptomic
mark
N
6-methyladenosine
(m6A)
is
the
most
common
type
of
messenger
RNA
(mRNA)
post-transcriptional
modification
in
eukaryotes.
With
discovery
demethylase
FTO
(FAT
MASS
AND
OBESITY-ASSOCIATED
PROTEIN)
Homo
Sapiens,
this
has
been
proven
to
be
dynamically
reversible.
technological
advances,
research
on
m6A
plants
also
rapidly
developed.
widely
distributed
plants,
which
usually
enriched
near
stop
codons
and
3'-UTRs,
conserved
sequences.
related
proteins
mainly
consist
three
components:
methyltransferases
(writers),
demethylases
(erasers),
reading
(readers).
regulates
growth
development
by
modulating
metabolic
processes
playing
an
important
role
their
responses
environmental
signals.
In
review,
we
briefly
outline
detection
techniques;
comparatively
analyze
distribution
characteristics
plants;
summarize
methyltransferases,
demethylases,
binding
m6A;
elaborate
how
functions
plant
growth,
development,
response
signals;
provide
a
summary
outlook
plants.
