Research status and challenges of plant-derived exosome-like nanoparticles

Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 174, С. 116543 - 116543

Опубликована: Апрель 11, 2024

In recent years, there has been an increasing number of related studies on exosomes. Most have focused exosomes derived from mammals, confirming the important role that play in cell communication. Plants, as a natural ingredient, plant-derived confirmed to similar structures and functions mammalian-derived Plant-derived exosome-like nanoparticles (PELNs) are lipid bilayer membrane nanovesicles containing bioactive constituents such miRNA, mRNA, protein, lipids obtained plant cells, can participate intercellular communication mediate transboundary communication, high bioavailability low immunogenicity, relatively safe, shown maintaining homeostasis preventing, treating variety diseases. this review, we describe biogenesis, isolation purification methods, structural composition, stability, safety, function PELNs challenges. The anti-inflammatory, antioxidant, antitumor drug delivery mainly described, status research exosome Chinese herbal medicines is outlined. Overall, summarized importance latest results field provided theoretical basis for future clinical application PELNs.

Язык: Английский

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Multifaceted Therapeutic Potential of Plant-Derived Exosomes: Immunomodulation, Anticancer, Anti-Aging, Anti-Melanogenesis, Detoxification, and Drug Delivery

Biomolecules, Год журнала: 2025, Номер 15(3), С. 394 - 394

Опубликована: Март 10, 2025

Most eukaryotic and prokaryotic cells have the potential to secrete a group of structures/membrane-bound organelles, collectively referred as extracellular vesicles (EVs), which offer several advantages producer/receiver cells. This review provides an overview EVs from plant sources with emphasis on their health-promoting possible use therapeutic agents. highlights essential biological effects plant-derived vesicles, including immune modulation, anticancer activities, protection against chemical toxicity pathogens, well anti-aging, anti-melanogenesis, anti-arthritic effects, along ongoing clinical studies. Evidence revealed that EVs’ contents exert beneficial properties through regulating important signaling pathways by transferring miRNAs other components. Taken all together, data proposed can be utilized nutritional compounds agents, such drug carriers. However, this emerging research area requires further in vitro/in vivo studies trials determine exact underlying mechanisms positive health treating various diseases.

Язык: Английский

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Recent Advances in the Isolation Strategies of Plant-Derived Exosomes and Their Therapeutic Applications

Current Issues in Molecular Biology, Год журнала: 2025, Номер 47(3), С. 144 - 144

Опубликована: Фев. 22, 2025

Exosome-like nanovesicles (ELNs) derived from natural products are gaining attention as innovative therapeutic agents due to their biocompatibility, low immunogenicity, and capability transport bioactive molecules such proteins, lipids, nucleic acids. These plant-derived ELNs exhibit structural similarities with mammalian exosomes, making them suitable for drug delivery, microbiome-targeted therapies, regenerative medicine. Recent studies highlight potential in treating cancer, inflammation, metabolic disorders. Additionally, have applications cosmetics, agriculture, the food industry. This review combines latest advancements research on ELNs, focusing isolation techniques, pharmacological effects, applications. Although offer promising opportunities, several challenges must be addressed, including standardization, large-scale production, vivo efficacy. By summarizing cutting-edge suggesting future directions, we aim inspire further development of next-generation platforms.

Язык: Английский

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Colon health benefits of plant-derived exosome-like nanoparticles via modulating gut microbiota and immunity

Critical Reviews in Food Science and Nutrition, Год журнала: 2025, Номер unknown, С. 1 - 21

Опубликована: Март 19, 2025

Exosomes are nanoscale particles with a lipid bilayer membrane that were first identified in mammalian cells. Plant-derived exosome-like nanoparticles (PELNs) share structural and molecular similarities exosomes, including lipids, proteins, microRNA (miRNA), plant-derived metabolites. Owing to their unique characteristics, such as outstanding stability, low immunogenicity, high biocompatibility, sustainability, PELNs have emerged promising natural bioactive agents the capacity for cross-kingdom cellular regulation. Dietary supplementation PELNs, particularly from fruits vegetables, has demonstrated health benefits. An increasing number of studies indicated beneficial effects on colon health. This review summarizes isolation characterization uptake, distribution after oral ingestion. Furthermore, this emphasizes interactions between gut microbiota, immune system, uptake by modulation bacteria metabolism, responses PELNs. Additionally, applications components drug carriers targeting reviewed. In summary, represent versatile approach improve health, potential both therapeutic preventive healthcare strategies.

Язык: Английский

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Tomato-derived extracellular vesicles increase intestinal zinc transportation by potentially down-regulating the expression of the metallothionein family

Food Research International, Год журнала: 2025, Номер 203, С. 115804 - 115804

Опубликована: Янв. 21, 2025

Язык: Английский

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Plant-derived nanovesicles and therapeutic application

Pharmacology & Therapeutics, Год журнала: 2025, Номер unknown, С. 108832 - 108832

Опубликована: Фев. 1, 2025

Язык: Английский

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Exosomes: A Promising Drug Delivery Tool in Hepatic Drug Delivery

Journal of Drug Delivery Science and Technology, Год журнала: 2025, Номер unknown, С. 106761 - 106761

Опубликована: Март 1, 2025

Язык: Английский

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Cutting-edge insights into the mechanistic understanding of plant-derived exosome-like nanoparticles: Implications for intestinal homeostasis

Food Research International, Год журнала: 2025, Номер unknown, С. 116186 - 116186

Опубликована: Март 1, 2025

Язык: Английский

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Extracellular Vesicle-Like Nanoparticles, Artificially Created by Heat Treatment of Euglena gracilis, Exhibit Autofluorescence and Suppress IL-6 Expression in RAW264.7 Cells

BioNanoScience, Год журнала: 2025, Номер 15(2)

Опубликована: Март 17, 2025

Язык: Английский

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Cost-effective isolation of Viburnum opulus-derived nanovesicles and evaluation of their cytotoxic, anticancer, and antioxidant properties on human glioblastoma cell line U87MG

Medical Oncology, Год журнала: 2025, Номер 42(4)

Опубликована: Март 17, 2025

Abstract Glioblastoma is the most common and highly invasive glial tumor, significantly reducing patient survival. Current therapeutic approaches have limited success rates. Plant-derived nanovesicles are a rapidly developing area, recognized for their exceptional biofunctional properties, emerging as promising approach in cancer treatment. The present study focuses on isolation of from Viburnum opulus fruits using cost-effective method that includes polymer-based exosome precipitation buffer size exclusion chromatography, followed by characterization. Morphological analysis via Field Emission Scanning Electron Microscopy Transmission revealed ranging oval to elliptical shapes, with average diameters 54.23 nm 41.21 nm, respectively. Dynamic light scattering determined 45.36 indicating presence nanovesicles, zeta potential was − 2.87 mV. Biochemical characterization showed total protein phenolic concentrations 1534 ± 97.78 µg/ml 4.270 0.66 mg gallic acid equivalents/L, respectively, antioxidant status values 3.83 0.37 mmol Trolox equivalents/L. Based IC50 values, these were 7.5 times more toxic U87MG human glioblastoma cells compared healthy dermal fibroblasts. Analyses including clonogenic cell survival, wound healing, apoptosis, status, oxidant continued only cells, fibroblasts low response nanovesicle Qualitative quantitative assessments demonstrated -derived effectively inhibited proliferation migration. Due non-toxic, anticancer, hold significant management. Graphical abstract

Язык: Английский

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